scholarly journals Метод количественного описания фиксированных гистологических образцов с помощью цифровой голографической микроскопии

2021 ◽  
Vol 47 (9) ◽  
pp. 18
Author(s):  
А.В. Белашов ◽  
А.А. Жихорева
Keyword(s):  
Quantitative Data ◽  
Digital Holographic Microscopy ◽  
Quantitative Characterization ◽  
Elastic Cartilage ◽  
Different Types ◽  
Phase Images ◽  
Automated Processing ◽  
Novel Method ◽  
Holographic Microscopy

A novel method for the quantitative characterization of fixed histological samples based on the statistical analysis of their phase images obtained using digital holographic microscopy is developed and presented. The proposed approach allows for fully automated processing of reconstructed phase images and obtaining quantitative data of morphological and optical characteristics of histological tissues structures. The method was validated on three histological samples of different types of tissues: ciliated columnar epithelium, elastic cartilage, and liver.

scholarly journals Measuring the Cell-Induced Deformation of Collagen Matrix Detected With Digital Holographic Microscopy

2007 ◽  
Author(s):  
Kaveh Azartash ◽  
Enrico Gratton
Keyword(s):  
Mammalian Cells ◽  
Collagen Matrix ◽  
Spatial Arrangement ◽  
Digital Holographic Microscopy ◽  
Physical Aspect ◽  
Reconstruction Algorithms ◽  
Phase Images ◽  
Holographic Microscopy ◽  
Set Up ◽  
Charged Couple Device

A modified Mach-Zender set-up in reflection is applied to record and reconstruct holographic amplitude and phase images. A charged couple device (CCD) is used to record a hologram and numerical reconstruction algorithms are then applied to rebuild the hologram for obtaining both phase and amplitude information. One could also focus on multiple focal planes from a single hologram, similar to the focusing control of a conventional microscope. The morphology and behavior of mammalian cells is determined by an interaction between signals from the intracellular matrix and the cellular responses. It is important to note that the physical aspect of the extracellular matrix is as significant as the chemical nature of it. Specifically the stresses, mechanical forces, and the profile of the external environment have major effects on cell behavior. The mechanical and physical characteristics of a tissue are greatly dependent on a hierarchical spatial arrangement of its extra-cellular matrix components. A key player in the ECM is collagen which exhibits significant tensile strength on the cellular scale. Digital holographic microscopy (DHM) is applied to study the deformation of collage matrix in response to cell migration.

Digital holographic microscopy (DHM) for metrology and dynamic characterization of MEMS and MOEMS

2006 ◽  
Author(s):  
Yves Emery ◽  
Etienne Cuche ◽  
François Marquet ◽  
Nicolas Aspert ◽  
Pierre Marquet ◽  
...  
Keyword(s):  
Digital Holographic Microscopy ◽  
Dynamic Characterization ◽  
Holographic Microscopy

scholarly journals Quantitative characterization of iridescent colours in biological studies: a novel method using optical theory

2018 ◽  
Vol 9 (1) ◽  
pp. 20180049 ◽  
Author(s):  
Hugo Gruson ◽  
Christine Andraud ◽  
Willy Daney de Marcillac ◽  
Serge Berthier ◽  
Marianne Elias ◽  
...  
Keyword(s):  
Test Cases ◽  
Biological Functions ◽  
Quantitative Characterization ◽  
Biological Studies ◽  
Measurement Protocol ◽  
Evolutionary Studies ◽  
Angular Dependency ◽  
Novel Method ◽  
Living Organisms

Iridescent colours are colours that change with viewing or illumination geometry. While they are widespread in many living organisms, most evolutionary studies on iridescence do not take into account their full complexity. Few studies try to precisely characterize what makes iridescent colours special: their angular dependency. Yet, it is likely that this angular dependency has biological functions and is therefore submitted to evolutionary pressures. For this reason, evolutionary biologists need a repeatable method to measure iridescent colours as well as variables to precisely quantify the angular dependency. In this study, we use a theoretical approach to propose five variables that allow one to fully describe iridescent colours at every angle combination. Based on the results, we propose a new measurement protocol and statistical method to reliably characterize iridescence while minimizing the required number of time-consuming measurements. We use hummingbird iridescent feathers and butterfly iridescent wings as test cases to demonstrate the strengths of this new method. We show that our method is precise enough to be potentially used at intraspecific level while being also time-efficient enough to encompass large taxonomic scales.

Dynamics and morphometric characterization of hippocampus neurons using digital holographic microscopy

2014 ◽  
Author(s):  
Saeid Elkatlawy ◽  
María Gomariz ◽  
Cristina Soto-Sánchez ◽  
Gema Martínez Navarrete ◽  
Eduardo Fernández ◽  
...  
Keyword(s):  
Digital Holographic Microscopy ◽  
Holographic Microscopy ◽  
Morphometric Characterization

scholarly journals In-depth investigation on quantitative characterization of pyrolysis oil by 31P NMR

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17567-17573 ◽  
Author(s):  
Haoxi Ben ◽  
Jack R. Ferrell III
Keyword(s):  
Quantitative Data ◽  
31P Nmr ◽  
Time Dependent ◽  
Pyrolysis Oil ◽  
Quantitative Characterization

The investigation on time-dependent changes when using 31P NMR to analyze pyrolysis bio-oils has been accomplished and the proposed application of this method is essential to achieve reliable quantitative data.

Novel Experimental-Computational Method for Quantitative Applications in Electronic Packaging

2001 ◽  
Author(s):  
Cosme Furlong ◽  
Ryszard J. Pryputniewicz
Keyword(s):  
Electronic Packaging ◽  
Quantitative Data ◽  
Experimental Methods ◽  
Computational Method ◽  
Electronic Packages ◽  
Quantitative Characterization ◽  
Design And Optimization ◽  
Combined Use ◽  
Microelectronic Components

Abstract Rapid advances in microelectronics require design and optimization of components and packages, for new and ever more demanding applications, in relatively short periods of time while satisfying electrical, thermal, and mechanical specifications, as well as cost and manufacturability expectations. In addition, reliability and durability have to be taken into consideration. As a consequence, some of the most sophisticated analytical, computational, and experimental methods are being used for development, optimization, and quantitative characterization of electronic packages. In this paper, a novel experimental-computational method, based on combined use of recent advances in laser-based optics and computational modeling, is described and its application is demonstrated by case studies of microelectronic components subjected to electro-thermo-mechanical loads. Results of these studies show that this methodology provides an effective engineering tool for nondestructive testing (NDT) applications in electronic packaging and provides indispensable quantitative data for development, optimization, and applications in electronic packaging.

Digital holographic microscopy for phase images of cervical cells 3D structure

2015 ◽  
Author(s):  
M. Mihailescu ◽  
I. A. Paun ◽  
E. I. Scarlat ◽  
I. Grigorescu ◽  
O. T. Nedelcu ◽  
...  
Keyword(s):  
3D Structure ◽  
Digital Holographic Microscopy ◽  
Phase Images ◽  
Holographic Microscopy ◽  
Cervical Cells
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