Influence of gelling agent on micropropagation cost and in vitro conservation of Turmeric (Curcuma longa) germplasm

2016 ◽  
Vol 3 (4) ◽  
Author(s):  
Anju Jain ◽  
R. P. Yadav
Keyword(s):  
Curcuma Longa ◽  
Shoot Multiplication ◽  
Cost Effective ◽  
In Vitro Conservation ◽  
Gelling Agent ◽  
Gelling Agents ◽  
Shoot Bud ◽  
Basal Salts ◽  
Regenerated Plantlets

To examine the effect of different gelling agents on micropropagation and cost effective in vitro conservation of C. longa cv. Sona cultures, six gelling agents viz. 7 g l−1 Agar (Himedia PT Pure), 2.5g l−1 Clarigel (Himedia), 4.5g l−1 Clarigar (Himedia), 6g l−1 Gelzen (Sigma), Isabgol 3.5g l−1 (Baidyanath) and 2.5g l−1 Phytagel (Sigma) were tested. Shoot bud explants excised from in vitro established cultures were inoculated on basal salts of Murashige and Skoog (MS) medium supplemented with 2.5 mg l−1 BAP + 3% sucrose. Highest rate of shoot multiplication without hyperhydric transformation was recorded with 2.88± 0.03 shoots/ explant in the cultures grown on media solidified with Clarigar, compared to regularly used gelling agent agar (2.31± 0.38). After 12 months of conservation, highest 85% survival of cultures was also recorded in the medium solidified with Clarigar, whereas only 50% of cultures survived on agar supplemented media. Regenerated plantlets were successfully acclimatized and produced healthy rhizomes using soil: sand: Farm Yard manure (2:1:1) with 85% survival.

scholarly journals Effect of gelling agents on in vitro development of Amelanchier canadensis ‘Rainbow Pillar’

2013 ◽  
Vol 19 (3-4) ◽  
Author(s):  
A. Fira ◽  
K. Magyar-Tábori ◽  
I. Hudák ◽  
D. Clapa ◽  
J. Dobránszky
Keyword(s):  
Guar Gum ◽  
Growth Parameters ◽  
Shoot Multiplication ◽  
Wheat Starch ◽  
Gelling Agent ◽  
Shoot Cultures ◽  
Gelling Agents ◽  
Shoot Number ◽  
The Media

In vitro shoot multiplication responses of Amelanchier canadensis ‘Rainbow Pillar’ were studied on media solidifi ed with different gelling agents. The media were gelled either with 6.8 g l-1 fi brous agar-agar, or 50.0 g l-1 wheat starch, or 20.0 g l-1 Guar gum, or 15 g l-1 Isubgol or 50.0 g l-1 wheat starch mixed with 0.5 g l-1 Phytagel. Shoot cultures were grown for two months, thereafter the multiplication rates (number of newly developed shoots per explant) were counted and the length of shoots were measured. We found that the highest shoot multiplication of Amelanchier canadensis ‘Rainbow Pillar’ occurred on media gelled with Guar gum, while the longest shoots developed on media with Starch. About four-fold shoot number were obtained on media with Guar gum compared to the weakest results found on media gelled with Isubgol. Finally, considering all factors (shoot growth parameters, costs) the most economical gelling agent for Amelanchier canadensis ‘Rainbow Pillar’ was proved to be wheat starch among the tested alternatives which allows a 75.6% cost reduction.

Cost-effective in vitro conservation of banana using alternatives of gelling agent (isabgol) and carbon source (market sugar)

2010 ◽  
Vol 32 (4) ◽  
pp. 703-711 ◽  
Author(s):  
Anuradha Agrawal ◽  
Rajkumari Sanayaima ◽  
Rajesh Tandon ◽  
Rishi K. Tyagi
Keyword(s):  
Carbon Source ◽  
Cost Effective ◽  
In Vitro Conservation ◽  
Gelling Agent

scholarly journals Effect of Bavistin and Adenine Sulphate on In vitro Shoot Multiplication of Picrorhiza scrophulariiflora

1970 ◽  
Vol 19 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Pranay Bantawa ◽  
Olivia Saha Roy ◽  
Parthadeb Ghosh ◽  
Tapan Kumar Mondal
Keyword(s):  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Virgin Soil ◽  
Adenine Sulphate ◽  
Multiple Shoot Buds ◽  
Picrorhiza Scrophulariiflora ◽  
Regenerated Plantlets

An alternative protocol for in vitro propagation of Picrorhiza scrophulariiflora is described using bavistin and adenine sulphate. The explants differentiated into multiple shoot buds on MS supplemented with various concentrations of bavistin and adenine sulphate ranging from 0 - 400 mg/l either alone or in combination. Maximum number of multiple shoots were obtained on MS containing the combination of bavistin (100 mg/l) and adenine sulphate (100 mg/l). In this combination as high as 28 shoots per explant was achieved and also vetrification of the cultures were not recorded. This study also demonstrates that the bavistin has stronger cytokinin-like activity than adenine sulphate. For instance, it was observed that bavistin alone in the concentration of 300 mg/l produced as high as 24 shoots per explant, however, adenine sulphate (100 mg/l) could produce a maximum of 18 shoots per explant. Moreover, higher or lower concentration did not improve the shoot multiplication. The microshoots were separated from the multiple shoots and transferred to MS containing various concentrations of auxins. Among them, NAA (1 mg/l) produced as high as 6 roots per explant. The regenerated plantlets were hardened in plastic cups (6 x 8 cm) containing 9 : 1 virgin soil and soil at Kyongnosla nursery and acclimated for four weeks. A 90% survival rate of the plants was recorded after 60 days. D.O.I. 10.3329/ptcb.v19i2.5441 Plant Tissue Cult. & Biotech. 19(2): 237-245, 2009 (December)

scholarly journals Vessel Volume, Gelling Agent, and Basal Salts Affect pH and Gel Strength of Autoclave Tissue Culture Media

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 683-685 ◽  
Author(s):  
Hazel Y. Wetzstein ◽  
Choongsik Kim ◽  
Harry E. Sommer
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Basal Medium ◽  
Gel Strength ◽  
Gelling Agent ◽  
Mean Values ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Vessel Volume ◽  
Basal Salts

Effects of autoclaving volume, gelling agent (Bactoagar, Gel-gro, Phytagar), and basal salts [Murashige and Skoog (MS); Woody Plant Medium (WPM); Gamborg B5 (GB)] on gel strength and pH of tissue culture media were tested. Gel strength was significantly affected by gelling agent and basal medium. MS media were generally softer than comparable WPM or GB media. As the vessel volume during autoclaving decreased, gel strength significantly decreased with Phytagar and Bactoagar gelling agents; Gel-gro had greater gel strength at the intermediate volume of medium autoclave. In all cases, autoclaving resulted in a pH decrease of 0.2 to 0.5 pH units. Lower pH values were associated with softer gels. The type of gelling agent did not greatly affect the postautoclave pH; mean values among gelling agents were within 0.05 pH units. Postautoclave pH of MS medium was lower than that of WPM or GB. This study verifies the need to observe uniform sterilization protocols to maintain consistency in the chemical and physical properties of media.

scholarly journals Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties

2019 ◽  
Vol 11 (4) ◽  
pp. 26
Author(s):  
Sai Krishna Repalli ◽  
Chaitanya Kumar Geda ◽  
N. S. N. Pradhan ◽  
G. J. N. Rao
Keyword(s):  
Culture Media ◽  
Indica Rice ◽  
Casein Hydrolysate ◽  
Gelling Agent ◽  
Regeneration Potential ◽  
Rice Varieties ◽  
Gelling Agents ◽  
Profound Influence ◽  
In Vitro Response

Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.

scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals In vitro Propagation and Plantlet Regeneration from Doritaenopsis Purple Gem ‘Ching Hua’ Flower Explants

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1256-1258 ◽  
Author(s):  
Wagner A. Vendrame ◽  
Ian Maguire ◽  
Virginia S. Carvalho
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Naphthalene Acetic Acid ◽  
Shoot Induction ◽  
Flower Buds ◽  
Flower Stem ◽  
Normal Flower ◽  
Regenerated Plantlets ◽  
Lateral Flower

The effects of four types of explants removed from 10-cm flower stalks of Doritaenopsis Purple Gem ‘Ching Hua’ (immature apical flower buds, immature lateral flower buds, flower stem nodes, and flower pedicel sections) and combinations of two plant growth regulators [naphthalene acetic acid (NAA) and thidiazuron (TDZ)] on direct in-vitro shoot induction and multiplication were studied. Immature apical flower buds were the only explants that showed induction and multiplication of shoots in vitro. NAA at 5.4 and 10.7 μm combined with either 4.5 or 9.1 μm TDZ provided the fastest and greatest percentages of shoot induction (27% to 40%) and the greatest numbers of shoot multiplication (111–160 shoots per explant). In vitro–induced shoots were rooted on medium containing 5.4 μm NAA and developed into plantlets with normal vegetative and reproductive morphology. Regenerated plantlets were acclimatized, showing 100% survival and establishment in greenhouse. Plantlets were grown to maturity and showed normal flower morphology. No floral off-types were observed. The high rates of shoot multiplication obtained offer a means for mass clonal propagation of this and possibly other related Doritaenopsis hybrids.

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals A comparative study between temporary immersion system and semi-solid cultures on shoot multiplication and plantlets production of two Moroccan date palm (Phoenix dactylifera L.) varieties in vitro

2020 ◽  
Vol 12 (2) ◽  
pp. 277-288
Author(s):  
Larbi ABAHMANE
Keyword(s):  
Date Palm ◽  
New Technologies ◽  
Shoot Multiplication ◽  
Handling Time ◽  
Cost Effective ◽  
Phoenix Dactylifera ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
Semi Solid

Date palm micropropagation is commonly performed on gelled media. However, it’s typically a labour-intensive system and consequently plantlets production cost is very high. Therefore, it is necessary to develop cost effective alternatives without compromising the quality of produced plant material. New technologies based on liquid media in bioreactors have been developed to reduce the handling time, while increasing the multiplication rates and plant quality. The present research focuses on the comparison between Temporary Immersion System (TIS) and gelled media (GM) culture systems of two Moroccan date palm varieties ‘Mejhool’ and ‘Boufeggous’. Obtained results indicated that shoot and root lengths as well as shoot fresh and dry weights were significantly (P < 0.05) higher in TIS compared to GM. Moreover, the vigour of obtained shoots was better in TIS compared to GM. Therefore, TIS-derived plantlets have shown an acclimatization rate of 95% while this rate for GM-derived plantlets was 82%. Hence, bioreactors, as a growing system based on TIS, can be a valid alternative to conventional systems for in vitro culture, resulting in a reduction of cost, shelving area requirements, labour and time for the mass propagation of date palm cultivars.

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