Secondary metabolite produced from marine bacterium Streptomyces sp. strain ND7c

2018 ◽  
Vol 54(5) ◽  
pp. 88
Author(s):  
Phuong, T.V. ◽  
Diep, C.N. ◽  
Lam, P.V.H. ◽  
Chu, D.X.
Keyword(s):  
Secondary Metabolite ◽  
Marine Bacterium ◽  
Streptomyces Sp

scholarly journals Draft Genome Sequence of Streptomyces sp. Strain PTY087I2, Isolated from Styela canopus , a Panamanian Tunicate

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Samantha M. Gromek ◽  
Anne A. Sung ◽  
Jonathan L. Klassen ◽  
Marcy J. Balunas
Keyword(s):  
Secondary Metabolite ◽  
Genome Sequence ◽  
Marine Bacterium ◽  
Draft Genome ◽  
Gene Clusters ◽  
Draft Genome Sequence ◽  
Nucleotide Identity ◽  
Average Nucleotide Identity ◽  
Bocas Del Toro ◽  
Streptomyces Sp

Streptomyces sp. PTY087I2 is a marine bacterium isolated from Styela canopus , a tunicate collected in Bocas del Toro, Panama. Here, we report a draft genome sequence for this bacterium, found to have 94.7% average nucleotide identity (ANI) with Streptomyces roseosporus NRRL 11379, and containing a diverse suite of secondary metabolite gene clusters.

Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

2021 ◽  
Vol 85 (3) ◽  
pp. 714-721
Author(s):  
Risa Takao ◽  
Katsuyuki Sakai ◽  
Hiroyuki Koshino ◽  
Hiroyuki Osada ◽  
Shunji Takahashi
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Response Regulator ◽  
Bac Library ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Gene Organization ◽  
Biosynthetic Gene ◽  
Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

Octalactins A and B: cytotoxic eight-membered-ring lactones from a marine bacterium, Streptomyces sp

1991 ◽  
Vol 113 (12) ◽  
pp. 4682-4683 ◽  
Author(s):  
Dianne M. Tapiolas ◽  
Mark Roman ◽  
William Fenical ◽  
Thomas J. Stout ◽  
Jon Clardy
Keyword(s):  
Marine Bacterium ◽  
Membered Ring ◽  
Streptomyces Sp

scholarly journals Complete Genome Sequence of Streptomyces sp. Sge12, Which Produces Antibacterial and Fungicidal Activities

2017 ◽  
Vol 5 (21) ◽  
Author(s):  
Jianguo Xu ◽  
Min Xu ◽  
Kai Liu ◽  
Qinyin Peng ◽  
Meifeng Tao
Keyword(s):  
Forest Soil ◽  
Secondary Metabolite ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gene Clusters ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Streptomyces Sp ◽  
Gram Positive Bacteria

ABSTRACT Streptomyces sp. Sge12 was isolated from forest soil and exhibited remarkable antimicrobial activities against selected fungi and Gram-positive bacteria. Here, we report the complete genome sequence of this strain, which contains 37 putative secondary metabolite gene clusters.

scholarly journals Bioassay Guided Fractionation of Marine Streptomyces sp. GMY01 and Antiplasmodial Assay using Microscopic and Flow Cytometry Method

2020 ◽  
Author(s):  
Ema Damayanti ◽  
Jaka Widada ◽  
Puspa Dewi N. Lotulung ◽  
Achmad Dinoto ◽  
Mustofa Mustofa
Keyword(s):  
Flow Cytometry ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Genome Mining ◽  
Vero Cells ◽  
Flash Chromatography ◽  
Main Constituent ◽  
Methanol Fraction ◽  
Streptomyces Sp ◽  
Bioassay Guided Fractionation

Genome mining study showed that marine-derived Streptomyces sp. GMY01 is a potential actinobacteria with abundant of secondary metabolite and anticancer activity. The study aimed to evaluate bioassay guided fractionation for antiplasmodial screening of GMY01 extract and to predict compound on active fractions. Ethyl acetate fraction was obtained from 11 days fermentation product of GMY01 and then it was fractionated using n-hexane and methanol solvent. Refractionated was applied using flash chromatography and column chromatography. Antiplasmodial assay was performed on Plasmodium falciparum FCR3 by microscopic method using thin blood smear + Giemsa stain, and flow cytometry method using SYBR Green I stain. Toxicity assay was performed on Vero cells line. Main constituent of active fraction was analyzed using LCMS/MS. The result of the study showed that ethyl acetate-methanol fraction has high antiplasmodial activity (IC50=3.96 µg/mL) with very low toxicity on Vero cells (IC50=30,072 µg/mL). Bioassay guided fractionation resulted F4.7 as highest Plasmodium inhibition (94.3% at 5 µg/mL) and was confirmed by microscopic and flow cytometry assay. Main constituent analysis showed C10H13NO (163.09971 Da) as mayor compound and predicted as nonribosomal polyketide synthetase (NRPS) secondary metabolite.

scholarly journals Critical review of fermentation and extraction of anti-Vibrio compounds from Streptomyces

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Loh Teng-Hern Tan ◽  
Learn-Han Lee ◽  
Bey-Hing Goh
Keyword(s):  
Secondary Metabolism ◽  
Secondary Metabolite ◽  
Bioactive Compounds ◽  
Critical Review ◽  
Antibacterial Agent ◽  
Bioactive Compound ◽  
Streptomyces Strain ◽  
Streptomyces Sp ◽  
Microbial Products ◽  
Fermentation Parameters

A single Streptomyces strain often have the potential to produce more than one bioactive compound. Fermentation parameters include media compositions, temperature and pH, have great impact on the secondary metabolism of Streptomyces and subsequently on production of different microbial products. This review aims to consolidate the studies on the cultivation parameters used to enhance the production of secondary metabolite with anti-Vibrio activity from a single Streptomyces strain. In turn, this review sheds light on the possible alterations of the cultivation parameters to obtain desired anti-Vibrio compounds from Streptomyces sp. Furthermore, the bioactive compounds with anti-Vibrio activity identified from Streptomyces sp. were demonstrated to exhibit immense values for future antibacterial agent developments.

ChemInform Abstract: Cyclomarins A-C, New Antiinflammatory Cyclic Peptides Produced by a Marine Bacterium (Streptomyces sp.).

ChemInform ◽  
2010 ◽  
Vol 31 (10) ◽  
pp. no-no
Author(s):  
Matthew K. Renner ◽  
Ya-Ching Shen ◽  
Xing-Chung Cheng ◽  
Paul R. Jensen ◽  
Walter Frankmoelle ◽  
...  
Keyword(s):  
Cyclic Peptides ◽  
Marine Bacterium ◽  
Streptomyces Sp
Sign in / Sign up

Export Citation Format

Share Document