scholarly journals EVALUATION OF IN-VITRO CYTOTOXIC ACTIVITY OF CHLOROFORM EXTRACT OF SIDA ACUTA BURM.F.

2017 ◽  
Vol 10 (2) ◽  
pp. 143 ◽  
Author(s):  
Lakshmi Kanta Kanthal
Keyword(s):  
Cervical Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Chloroform Extract ◽  
Epidermoid Carcinoma ◽  
Sida Acuta ◽  
Human Cervical Cancer Cell ◽  
Human Epidermoid Carcinoma ◽  
Human Cervical Cancer

ABSTRACTObjective: The aim of the present work was to investigate the in-vitro cytotoxic activity of chloroform extract of Sida acuta Burm.f.Methods: The in-vitro cytotoxic activity of chloroform extract was performed by MTT assay method against A-431 (Human epidermoid carcinoma) and HeLa (Human cervical cancer) cell lines.Results: The various concentration of crude chloroform extract (1000, 500, 250, 125, 62.5 µg/ml concentration) of Sida acuta whole plant was performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against A-431 cell lines (human epidermoid carcinoma) with an of 375±0.00 and HeLa cell lines (human cervix carcinoma with an IC50 (inhibit cell growth by 50%) 610.00±2.5. The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the extract indicates that Sida acuta has anticancer activity against A-431-Human epidermoid carcinoma, HeLa-Human cervical cancer cell lines.Conclusion: The present study concluded that the chloroform extract of Sida acuta Burm.f. posses potent cytotoxic activity.Keywords: Sida acuta Burm.f., Chloroform extract, Cytotoxic activity, IC50.      

scholarly journals Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 256 ◽  
Author(s):  
Yiqiao Liu ◽  
Jiang Qian ◽  
Zhihua Sun ◽  
Dongting Zhangsun ◽  
Sulan Luo
Keyword(s):  
Cervical Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Cervical Cancer Cell ◽  
Nicotinic Acetylcholine ◽  
Nachr Subtype ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Nicotinic acetylcholine receptors (nAChRs) are associated with various cancers, but the relation between nAChRs and cervical cancer remains unclear. Therefore, this study investigated the differential expression of nAChR subunits in human cervical cancer cell lines (SiHa, HeLa, and CaSki) and in normal ectocervical cell lines (Ect1/E6E7) at mRNA and protein levels. Two specific nAChR subtype blockers, αO-conotoxin GeXIVA and α-conotoxin TxID, were then selected to treat different human cervical cancer cell lines with specific nAChR subtype overexpression. The results showed that α3, α9, α10, and β4 nAChR subunits were overexpressed in SiHa cells compared with that in normal cells. α9 and α10 nAChR subunits were overexpressed in CaSki cells. α*-conotoxins that targeted either α9α10 or α3β4 nAChR were able to significantly inhibit cervical cancer cell proliferation. These findings may provide a basis for new targets for cervical cancer targeted therapy.

Su.71. MHC Class-I Related Chain B and Not Chain A is Preferentially Expressed on Human Cervical Cancer Cell Lines

2008 ◽  
Vol 127 ◽  
pp. S147
Author(s):  
Luis Jave-Suarez ◽  
Naela Arreygue-Garcia ◽  
Adriana Aguilar-Lemarrroy ◽  
Miriam Jimenez-Perez ◽  
Angel Arregui ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Mhc Class I ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Class I ◽  
Cervical Cancer Cell ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

scholarly journals An in vitro Evaluation of Apigenin and Apigenin-7-O-glucoside Against HeLa Human Cervical Cancer Cell Line

2020 ◽  
Vol 71 (2) ◽  
pp. 140-144
Author(s):  
Daliana Minda ◽  
Stefana Avram ◽  
Ioana Zinuca Pavel ◽  
Brigitta Kis ◽  
Alexandra Ghitu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Experimental Conditions ◽  
Human Cervical Cancer Cell ◽  
Cervical Cancer Cells ◽  
Apoptotic Effects ◽  
Human Cervical Cancer

pigenin (API) is a phytocompound belonging to the subclass of flavones that can be found in both functional foods as well as medicinal plants. Recent studies have assigned API antioxidant, anti-inflammatory, anti-spasmodic, anti-viral, anti-thrombotic, anti-angiogenic and chemopreventive potential in vitro on various cell lines and/or in experimental animal models. Apigenin-7-O-glucoside (API-7) is one of its main glycosides and can be commonly found in chamomile flowers, parsley, celery. The aim of this study was to evaluate the in vitro anti-proliferative and pro-apoptotic effects of API and its glycoside (apigenin-7-O-glucoside) against HeLa human cervical cancer cells. Results have shown that in the set experimental conditions both the aglycone as well as the heteroside elicit antiproliferative and pro-apoptotic potential against the screened cell line, the aglycone being more active than the heteroside.

scholarly journals Effect of sodium dichloroacetate as single agent or in combination with cisplatin in normal and human cervical cancer cell lines

2020 ◽  
Vol 19 (3) ◽  
pp. 467-474
Author(s):  
Alejandro Zugasti ◽  
Ana L. Rivera ◽  
Sonia Y. Silva ◽  
Miguel A. Alfaro ◽  
Crystel A. Sierra
Keyword(s):  
Cervical Cancer ◽  
Cell Viability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Hela Cells ◽  
Cancer Cell Lines ◽  
Peripheral Blood Mononuclear ◽  
Hek 293 ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Purpose: To evaluate the synergistic cytotoxicity of sodium dichloroacetate (DCA) in combination with cisplatin (CIS) against human cervical cancer cell lines. Methods: Cervical cancer SiHa and HeLa cells and normal cells (Hek-293, Vero, peripheral blood mononuclear and human erythrocytes) were treated in vitro with DCA and CIS individually or their combination. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method while hemolytic activity was evaluated from the released hemoglobin. Halfmaximal inhibitory concentration (IC50) of DCA or CIS was obtained. Results: The combination of DCA + CIS decreased the cell viability of SiHa, Hek-293, Vero, and PBMC cells, but not of Hela cells (p < 0.05). Furthermore, the individual treatments alone or in combination did not cause significant hemolysis (p < 0.05). Conclusion: The combination of DCA + CIS increases the damage caused by CIS alone on SiHa cells. It also decreases the cell viability of Hek-293 and Vero without affecting peripheral blood mononuclear and human erythrocyte integrity. The results suggest that the combination of DCA and CIS can induce synergistic antitumor effect in different types of cancer cell lines. However, further studies are required to determine the biological effects of the combination of DCA and CIS in vivo. Keywords: Cervical cancer, Sodium dichloroacetate, Cisplatin, Viability, Hemolysis

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