scholarly journals ANALYSIS OF ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENT AND TOTAL FLAVONOID CONTENT OF LANTANA CAMARA LEAVES AND FLOWERS

Author(s):  
Jigisha Anand ◽  
Shanu Chaudhary ◽  
Nishant Rai

 Objective: The present study aims at screening phytochemicals present in Lantana camara leaves and flower extracts.Methods: Methanolic extracts of leaves and flower of L. camara were prepared by the solvent extraction method. The preliminary qualitative photochemical analysis of leaves and flower extract was done following standard procedures. For quantitative phytochemical screening, total phenolic content (TPC), total flavonoid content (TFC), and free radical scavenging activity of L. camara was estimated.Results: The preliminary qualitative analysis showed the presence of flavonoids, phenols, terpenoids, anthocyanins, carbohydrates, and proteins in the methanolic extracts of Lantana leaves and flower, while saponins, glycosides, and alkaloids were not detected in both the extracts. Quantitative assays were done for determination of TPC, TFC, and free radical scavenging activity of L. camara. The methanolic extract of Lantana flowers depicted highest TFC (15.76±0.005 mg of hydrated catechin equivalent/ml of sample) and TPC (17±0.005 mg of Gallic acid equivalents equivalent/ml of sample), while both leaves and flower extracts revealed extortionately high 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging activity.Conclusion: The present work depicted that L. camara leaves and flowers are rich in antioxidant activity and have a promising application as nutraceuticals for herbal drug formulations.

2019 ◽  
Vol 8 (6) ◽  
pp. 94
Author(s):  
Y. Riswahyuli ◽  
Abdul Rohman ◽  
Francis M. C. S. Setyabudi ◽  
Sri Raharjo

Several wild honey samples collected from seven different regions in Indonesia were investigated to determine their total phenolic content, flavonoid content, and free radical scavenging activity by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DDPH) radical and phenolic profile. Rutin, (+)-catechin, ferulic acid, and galangin were found to be the major phenolic compounds of Indonesia wild honey. The total phenolic content significantly correlated with the total flavonoid content (p=0.000) and the percentage of DPPH radical scavenging activity (p=0.000). Results indicated that there are different polyphenol profiles among the different regions.


2019 ◽  
Vol 886 ◽  
pp. 61-69
Author(s):  
Suriyan Sukati ◽  
Warachate Khobjai

Turmeric (Curcuma longa L.), a member of Zingiberacaeae, is used for Thai traditional medicine, flavoring, preservative, and coloring agent. In Southern Thailand, turmeric is a famous ingredient for food recipes. Not only is the ripened rhizome used for cuisine, but also the young rhizomes. Previous studies have indicated that a variety of turmeric products from the ripened rhizome are valuable source of antioxidant compounds. However, there are ambiguous data regarding the level of antioxidant activity of the young rhizome. The aim of this study was to investigate the level of total phenolic content (TPC) and antioxidant activity of the young rhizomes by comparing to the ripened rhizome. TPC and antioxidant activity of aqueous and 80% methanolic extracts from the young turmeric grown in Southern Thailand were estimated using the Folin-Ciocalteu method and DPPH free radical scavenging activity (DPPH-RSA) assay, respectively. Results showed that the concentration of TPC determined in methanolic extract (19.80±0.25 mg GAE/ g extract) from the young rhizome was significantly higher than the corresponding aqueous extract (18.38±0.41 mg GAE/ g extract). The aqueous and methanolic extracts exhibited significant inhibition in DPPH-RSA with the half-maximal inhibitory concentration (IC50) value of 5.88±0.70 mg/mL and 3.00±0.31 mg/mL, respectively. However, in comparison with the ripened rhizome, TPC and DPPH-RSA of the young rhizome were lower. These results indicate that the young rhizome grown in Southern Thailand could be a source of antioxidant compounds, but the ripened rhizome provides better antioxidant properties than the young rhizome.


2021 ◽  
Vol 913 (1) ◽  
pp. 012042
Author(s):  
N Ardiana ◽  
A S Abidin ◽  
B T K Ilhami ◽  
A L Sunarwidhi ◽  
S Widyastuti ◽  
...  

Abstract Seaweed is one of the marine-biota that is widely known for its uses, one of them is in the food functional. Functional food is a program that promotes a healthy diet so that it can prevent a disease when consuming food. With functional food, daily eating patterns can be maintained and also the content of the food consumed daily has a good effect on the body. It is known that in seaweed, especially brown algae (S. crassifolium), it contains antioxidants and other compounds that are good for the health. In this study, phytochemical content and proximate composition was evaluated in S. crassifolium. Including total flavonoid content and total phenolic content, antioxidant capacity both of DPPH and ABTS assay used three type of solvents such as Ethanol, n-Hexana, and ethyl acetate for comparable the best solvents. Also, proximate analysis to determine the content of moisture content, crude fat content, crude protein content, and carbohydrates content. The results showed that n-Hexana and ethyl acetate solvents were significantly than ethanol. Which mean both of them had highest values of total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity. The results of chemical composition in proximate compounds showed that S. crassifolium was suitable to be candidate of functional food.


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