scholarly journals COMPARISON OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME AND OSBECKIA RETICULATA BEDD. USING ANIMAL MODELS

2019 ◽  
Vol 12 (1) ◽  
pp. 106
Author(s):  
Bosco Lawarence ◽  
Murugan K
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Callus Formation ◽  
Naphthalene Acetic Acid ◽  
Immersion Method ◽  
Hot Plate ◽  
Writhing Test ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Objective: The objective of the present work is to isolate, purify, and fractionate anthocyanin from selected Osbeckia species and also to compare the analgesic and anti-inflammatory potentiality using animal models.Methods: Methodologies include extraction of anthocyanin from the in vitro callus culture of Osbeckia aspera and Osbeckia reticulata, purification using amberlite column chromatography, and fractionation by liquid chromatography-tandem mass spectrometry. The analgesic activity was determined by tail immersion method, analgesy meter, hot plate, and acetic acid-induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced paw inflammation in mice.Results: Anthocyanin-producing callus cultures were established in MS medium fortified with various combinations of phytohormones and sucrose. Optimal callus formation in O. aspera was initiated on cultures containing 0.5 mg/L of 2, 4-D, and 0.5 mg/L 6-benzylaminopurine (BA). In O. reticulata callus was initiated in the presence of 1.2 mg/L BA and 1.4 mg/L naphthalene acetic acid. The same hormonal combination on extended treatments turned the white friable callus into red compact callus. Anthocyanins obtained from Osbeckia species were purified and fractionated containing malvidin-3-diglucoside, delphinidin, cyanidin aglycone, and peonidin. Purified anthocyanin of O. aspera at the concentrations 50, 100, 150, and 200 mg/kg after 120 min exhibited significant analgesic activity by tail immersion method, in comparison to O. reticulata. However, with hot plate method, anthocyanin of O. reticulata produced a significant analgesic activity even at lower doses (50 and 100 mg/kg) after 120 min. However, in writhing test, theepurified anthocyanin of O. aspera significantly stopped the number of writhes at a dose of 200 mg/kg and also by the anthocyanin of O. reticulata. In the evaluation of anti-inflammatory effect using plethysmometer, anthocyanin at doses of 100,150 and 200 mg/kg started producing anti-inflammatory effect after 30 min, which lasted until 120 min.Conclusion: It is concluded from the present study that the purified anthocyanin of Osbeckia possesses potent analgesic and anti-inflammatory activities.

scholarly journals COMPARISON OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME AND OSBECKIA RETICULATA BEDD. USING ANIMAL MODELS

2019 ◽  
Vol 12 (1) ◽  
pp. 106 ◽  
Author(s):  
Bosco Lawarence ◽  
Murugan K
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Callus Formation ◽  
Naphthalene Acetic Acid ◽  
Immersion Method ◽  
Hot Plate ◽  
Writhing Test ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Objective: The objective of the present work is to isolate, purify, and fractionate anthocyanin from selected Osbeckia species and also to compare the analgesic and anti-inflammatory potentiality using animal models.Methods: Methodologies include extraction of anthocyanin from the in vitro callus culture of Osbeckia aspera and Osbeckia reticulata, purification using amberlite column chromatography, and fractionation by liquid chromatography-tandem mass spectrometry. The analgesic activity was determined by tail immersion method, analgesy meter, hot plate, and acetic acid-induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced paw inflammation in mice.Results: Anthocyanin-producing callus cultures were established in MS medium fortified with various combinations of phytohormones and sucrose. Optimal callus formation in O. aspera was initiated on cultures containing 0.5 mg/L of 2, 4-D, and 0.5 mg/L 6-benzylaminopurine (BA). In O. reticulata callus was initiated in the presence of 1.2 mg/L BA and 1.4 mg/L naphthalene acetic acid. The same hormonal combination on extended treatments turned the white friable callus into red compact callus. Anthocyanins obtained from Osbeckia species were purified and fractionated containing malvidin-3-diglucoside, delphinidin, cyanidin aglycone, and peonidin. Purified anthocyanin of O. aspera at the concentrations 50, 100, 150, and 200 mg/kg after 120 min exhibited significant analgesic activity by tail immersion method, in comparison to O. reticulata. However, with hot plate method, anthocyanin of O. reticulata produced a significant analgesic activity even at lower doses (50 and 100 mg/kg) after 120 min. However, in writhing test, theepurified anthocyanin of O. aspera significantly stopped the number of writhes at a dose of 200 mg/kg and also by the anthocyanin of O. reticulata. In the evaluation of anti-inflammatory effect using plethysmometer, anthocyanin at doses of 100,150 and 200 mg/kg started producing anti-inflammatory effect after 30 min, which lasted until 120 min.Conclusion: It is concluded from the present study that the purified anthocyanin of Osbeckia possesses potent analgesic and anti-inflammatory activities.

scholarly journals Evaluation of Analgesic and Anti-inflammatory Activities of Polyalthia simiarum (Hook. F. &Thomson)

2019 ◽  
Vol 5 (1) ◽  
pp. 18-23
Author(s):  
Selina Kabir ◽  
Ronok Zahan ◽  
Abdullah Mohammad Sarwaruddin Chowdhury ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Abdur Rashid
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Biological Activities ◽  
Dependent Manner ◽  
Paw Edema ◽  
Immersion Method ◽  
Ongoing Research ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Dose Dependent

Background: Polyalthia simiarum (Hook. F. &Thomson) exhibits different effects in human body. Objective: As a part of ongoing research on medicinal plants of Bangladesh, the present study is focused to investigate the analgesic and anti-inflammatory activities of stem bark of Polyalthia simiarum (Annonaceae). Methodology: The ethyl acetate (EA) and petroleum ether (PE) extracts were subjected to qualitative chemical investigation for the identification of different phytoconstituents. The analgesic activity was determined for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test. The anti-inflammatory activity was evaluated by carrageenan induced paw edema in rats. Analgesic and anti-inflammatory data were evaluated statistically analysed by Dunnett’s-T test. Result: Both extracts at the dose of 50- and 100 mg/kg b.w., produced significant increase in pain threshold in tail immersion method whereas significantly reduced the writhing caused by acetic acid in a dose dependent manner. The EA and PE extracts showed anti-inflammatory activities at 50- and 100 mg/kg body weight. Among all the extracts, the EA extract showed a dose dependent and comparable analgesic activity in all the tested methods and also reduced the paw edema considerably (27.5% and 39.1% inhibition after 4h), in dose dependent manner when compared to carrageenan induced control rat. Conclusion: Therefore, the EA and PE extracts of Polyalthia simiarum were capable to exhibit moderate analgesic and anti-inflammatory activities. This is the first report of analgesic and anti-inflammatory potential of Polyalthia simiarum and can be further investigated to isolate the active compounds responsible for the biological activities. Journal of National Institute of Neurosciences Bangladesh, 2019;5(1): 18-23

scholarly journals Analgesic, Anti-Inflammatory, and GC-MS Studies onCastanospermum australeA. Cunn. & C. FraserexHook.

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Thankarajan Sajeesh ◽  
Thangaraj Parimelazhagan
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanol Extract ◽  
Hot Plate ◽  
Plate Method ◽  
Writhing Test ◽  
Paw Oedema ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Ethanol Extracts

The present study was aimed to evaluate the analgesic and anti-inflammatory properties ofCastanospermum australeand to profile phytochemicals by GC-MS. The ethanolic extracts were prepared by successive solvent extraction using Soxhlet apparatus. The analgesic activity was analyzed by hot plate method and acetic acid-induced writhing test whereas anti-inflammatory study was done by carrageenan induced paw oedema model. The acute toxicity study revealed that ethanol extracts of leaf and bark ofC. australewere safe even at a higher dose of 2000 mg/kg whereas ethanol extract of seed was toxic at the same dose. In both hot plate method (5.85 s) and acetic acid-induced writhing test (57%), the leaf ethanol extract exhibited significant analgesic activity (P<0.001) at a dose of 400 mg/kg. The anti-inflammatory activity of leaf extract was exhibited by the reduction in paw linear diameter by 64.76% at 400 mg/kg in carrageenan induced paw oedema. The GC-MS analysis of the ethanol extract of leaf revealed sixteen major compounds of which 1,7-dimethyl-4,10-dioxa-1,7-diazacyclododecane, (+)-N-methylephedrine, and permethylspermine were found to be pharmaceutically and the most important. These findings justify thatC. australecan be a valuable natural analgesic and anti-inflammatory source which seemed to provide potential phytotherapeutics against various ailments.

Extraction and evaluation of anti-inflammatory and analgesic activity of Mimosa rubicaulis in Swiss albino rats

2020 ◽  
Vol 18 ◽  
Author(s):  
Roshani Gurung ◽  
Sundar Adhikari ◽  
Niranjan Koirala ◽  
Kalpana Parajuli
Keyword(s):  
Acute Toxicity ◽  
Analgesic Activity ◽  
Antiinflammatory Activity ◽  
Albino Rats ◽  
Paw Edema ◽  
Immersion Method ◽  
Hot Plate ◽  
Acute Toxicity Study ◽  
Tail Immersion ◽  
Anti Inflammatory

Background: Pain and inflammation are major health problems. The adverse effect of synthetic drugs for the management of painful inflammatory conditions gives a strong motivation for researchers to develop newer drugs with a lesser side effect. Mimosa rubicaulis has been used traditionally in rheumatism, fungal infection, wound, fever, etc. Objective: The objective was to evaluate the anti-inflammatory and analgesic activity of ethanolic stem extract of M. rubicaulis in Swiss albino rats. Methods: The stem of M. rubicaulis were collected, subjected to double maceration using ethanol as a solvent. Then, swiss albino rats (n=72) were taken. An acute toxicity study was conducted using different doses of plant extract. The antiinflammatory activity (100 mg/kg, 250 mg/kg) was evaluated using the carrageenan-induced paw edema test. While, analgesic activity (100 mg/kg, 250 mg/kg) was evaluated using a hot plate and tail immersion method. The result was analyzed using a one-way Analysis of variance and post hoc analysis with the Tukey test. Results: The acute toxicity study showed that the plant is safe for use in an animal model. The extract showed antiinflammatory activity by a reduction in paw edema volume i.e. 25%, and 48%. Similarly, analgesic activity was shown by an increase in latency of paw licking and tail withdrawal by 41%, 56% (hot plate method) and 39%, and 54% (Tail immersion method), p<0.05. Conclusion: It can be concluded that ethanolic extract of Mimosa rubicaulis is safe and possesses both anti-inflammatory and analgesic activity.

Assessment of the antioxidant, thrombolytic, analgesic, anti-inflammatory, antidepressant and anxiolytic activities of leaf extracts and fractions of Tetracera sarmentosa (L.) Vahl

2018 ◽  
Vol 29 (1) ◽  
pp. 81-93 ◽  
Author(s):  
Mir Muhammad Nasir Uddin ◽  
Mohammad Shah Hafez Kabir ◽  
Mahmud Hasan ◽  
Zobaer Al Mahmud ◽  
N. M. Mahmudul Alam Bhuiya ◽  
...  
Keyword(s):  
Flowering Plant ◽  
Clot Lysis ◽  
Leaf Extracts ◽  
Immersion Method ◽  
Pure Ethanol ◽  
Thrombolytic Activity ◽  
Writhing Test ◽  
Analgesic Effects ◽  
Tail Immersion ◽  
Anti Inflammatory

AbstractBackground:The plant under investigation (Tetracera sarmentosa) is a dicotyledonous flowering plant and belongs to the family Dilleniaceae. The goal of our investigation was to determine whether the leaf extracts of this plant held any significant medicinal properties.Methods:Leaves ofT. sarmentosawere extracted with pure ethanol (EETS) and methanol (METS), and then methanol extract fractioned withn-hexane (NHFMETS) and chloroform (CHFMETS). The extracts and fractions were tested for antioxidant activity, which was measured by using qualitative and quantitative procedures. Thrombolytic activity was evaluated by the clot lysis test. Analgesic activity was evaluated employing the acidic acid-induced writhing test, the formalin-induced paw licking test and tail immersion on Swiss albino mice. The anti-inflammatory activity test was studied using the paw edema test. Forced swimming, tail suspension, elevated plus maze and hole board model tests were used to evaluate neuropharmacological activity.Results:All the extracts and fractions possessed antioxidant effects. All the extracts, fractions and streptokinase exhibited significant (p<0.0001) clot lysis. The extracts and fractions produced significant analgesic effects as evaluated by the acetic acid writhing test, the formalin-induced paw licking test and the tail immersion method. Similarly, carrageenan-induced inflammation was significantly antagonized by the treatments. The extracts and fractions also significantly showed neuropharmacological (antidepressant and anxiolytic) effects.Conclusions:The overall results suggested that this plant deserves further investigation to isolate the active compounds which are responsible for these activities and to establish the mechanism of action.

Pharmacological Evaluation of Antipyretic, Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Cassia fistula

2021 ◽  
Author(s):  
D.K. Sharma ◽  
S.K. Sharma ◽  
M.K. Lonare ◽  
Rajdeep Kaur ◽  
V.K. Dumka
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Control Group ◽  
Albino Rats ◽  
Hot Plate ◽  
Antipyretic Activity ◽  
Tail Immersion ◽  
Anti Inflammatory

Background: The antipyretic, analgesic and anti-inflammatory activities of two concentrations (100 and 200 mg/kg) of ethanolic extract of leaf, bark, flower and fruit pulp of C. fistula were determined in male wistar albino rats. Methods: Antipyretic activity was assessed by E. coli endotoxin induced pyrexia. Analgesic activity was assessed by hot plate, tail immersion and acetic acid induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced rat paw edema assay. Result: Significant (p less than 0.05) antipyretic activity was exhibited from 2h onwards by bark extract @ 200 mg/kg and from 3h onwards by bark extract @100 mg/kg and leaves extract @ 200mg/kg as compared to control group. Significant (p less than 0.05) analgesic activity was shown by extract of bark @ 200 mg/kg as it is evident by increase in reflex time in hot plate (90,120,180 min), tail immersion test (120,180 min) and inhibition of writhing (32.12%). Significant (p less than 0.05) anti-inflammatory activity was exhibited from 3h post administration by bark @ 200 and leaves @ 100 and 200 mg/kg.

scholarly journals In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Shahriar Ahmed ◽  
Ahmed Rakib ◽  
Md. Ashiqul Islam ◽  
Bibi Humayra Khanam ◽  
Farhana Binta Faiz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Acetic Acid ◽  
Molecular Docking ◽  
Analgesic Activity ◽  
In Silico ◽  
Antineoplastic Activity ◽  
Paw Edema ◽  
Writhing Test ◽  
Antipyretic Activity ◽  
Anti Inflammatory

Abstract Background The study was conducted to evaluate the analgesic, anti-inflammatory, antipyretic properties of ethanolic extract of Tacca integrifolia rhizome (EETI) in mice and cytotoxic effects in brine shrimp nauplii followed by a PASS prediction study for some isolated compounds of T. integrifolia. Additionally, this experiment included the in silico molecular docking and ADME/T property analyses of some phytochemicals. Methods Formalin- induced paw licking test and acetic acid-induced writhing test for analgesic activity, carrageenan-induced paw edema test for anti-inflammatory potential and Brewer’s yeast-induced pyrexia test for antipyretic activity were applied. Antinociceptive and antineoplastic activity for breast cancer were revealed with PASS program. Schrodinger suite 2015 was used to evaluate the binding interaction and ADME/T properties of selected phytoconstituents with estrogen receptor alpha. Results In formalin-induced paw licking test, EETI at the doses of 200 and 400 mg/kg BW showed highly significant inhibition of writhing in both neurogenic and inflammatory phases. While EETI also exhibited highly significant, compared to control, writhing inhibition for both the doses in acetic acid-induced writhing test. Moderate anti-inflammatory effect at a dose of 400 mg/kg BW was noticed in paw-edema test. It also showed 77.51% of maximum antipyretic effect which was significantly effective compared to standard drug paracetamol (150 mg/kg) in Brewer’s yeast-induced pyrexia test. The EETI showed potential cytotoxic activity with LC50 value of 114.46 μg/mL. The PASS prediction revealed the potential antinociceptive and antineoplastic activity of target compounds. The compounds betulinic acid, quercetin-3-α-arabinoside, catsanogenin were found to be effective in molecular docking study. Conclusion It is evident that the EETI possesses highly significant analgesic activity with remarkable anti-inflammatory and antipyretic activity. The phytoconstituents have potential antinociceptive and antineoplastic (breast cancer) activity. Graphical abstract

scholarly journals Evaluation of analgesic, neuropharmacological and cytotoxic activity of Trigonella foenum-graecum Linn.

1970 ◽  
Vol 1 (1) ◽  
pp. 6-11
Author(s):  
Moli Akter ◽  
Mirola Afroze ◽  
Ambia Khatun
Keyword(s):  
Acetic Acid ◽  
Brine Shrimp ◽  
Dependent Manner ◽  
Immersion Method ◽  
Standard Drug ◽  
Writhing Test ◽  
Trigonella Foenum Graecum ◽  
Vincristine Sulphate ◽  
Tail Immersion ◽  
Dose Dependent

The present study was carried out to investigate the possible analgesic, neuropharmacological and cytotoxic activities of the methanolic extract of Trigonella foenum-graecum Linn. leaves. The analgesic and neuropharmacological activities of Trigonella foenum-graecum Linn. were investigated at the doses of 100mg/kg, 200mg/kg and 400mg/kg of body weight in mice. Analgesic potential of the extract was evaluated for centrally acting analgesic property using tail immersion method and peripheral analgesic actions using acetic acid-induced writhing test. In acetic acid-induced writhing test, extract produced a significant (p < 0.001) inhibition of writhing response in a dose dependent manner but maximum inhibition (93.46%) of writhing was found at 400mg/kg dose. In tail immersion method, extract caused a significant (p < 0.001) increase in latency time and the results were comparable to the standard drug Diclofenac- Sodium. In addition, neuropharmacological property of crude extract was carried out by Hole cross and Open field test. The extract significantly (p < 0.05-0.001) displayed a dose dependent suppression of motor activity, exploratory behaviour. Furthermore, the extract was subjected to Brine Shrimp lethality bioassay for primary evaluation of cytotoxicity, where the extract was found to be highly toxic to Brine Shrimp nauplii, having LC50 values of 10μg/ml while the LC50 of the reference anticancer drug vincristine sulphate was 0.66μg/ml. The results of this present study suggest that the extract possesses analgesic, cytotoxic and CNS depressant activities. Key Words: Trigonella foenum-graecum Linn.; cytotoxicity; neuropharmacological; analgesic activity. DOI: http://dx.doi.org/10.3329/icpj.v1i1.9218 International Current Pharmaceutical Journal 2011, 1(1): 6-11

scholarly journals In vivo and in silico evaluation of analgesic activity of Lippia alba

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Md. Abdullah Aziz ◽  
Manna Mehedi ◽  
Mst. Irin Akter ◽  
Sadiur Rahman Sajon ◽  
Kishor Mazumder ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
In Silico ◽  
Cyclooxygenase 2 ◽  
Latency Time ◽  
Hot Plate ◽  
Standard Drug ◽  
Lippia Alba ◽  
Tail Immersion

Abstract Background This study was conducted to evaluate the analgesic activity of different extracts of Lippia alba (L. alba) along with in silico evaluation of analgesic activity of the isolated compounds from L. alba against cyclooxygenase-2 enzyme and ADME/T analysis of isolated compounds. Method In vivo analgesic activity of different extracts of L. alba was evaluated by acetic acid-induced writhing, tail immersion and hot plate on Swiss albino mice of either sex. In silico activity of the isolated compounds and ADME/T analysis were performed by Schrödinger-Maestro (Version 10.1) and OSIRIS Data warrior (version 4.6.1) softwares. Results Three different extracts (Methanolic extract: ME; Petroleum ether extract: PEE; Dichloromethane extract: DCME) of 250 mg/kg and 500 mg/kg doses were used in the experiments to evaluate analgesic activity. In acetic acid-induced writhing test, significant results were seen for PEE (500 mg/kg) and DCME (500 mg/kg), which were 53.09 ± 2.87 & 50.09 ± 4.24%, respectively. In tail immersion test, the best latency time was found at + 60 min for PEE (500 mg/kg) which is (5.65 ± 0.25) sec. For hot plate test, DCME at a dose 500 mg/kg showed the highest increase in latency time, which was 13.48 ± 0.33 s. In the case of in silico evaluation of analgesic activity, the compounds such as geranial, neral, (E)-caryophyllene, caryophyllene oxide, mussaenide, and 8-epi-loganin meet the condition of Lipinski’s rule of five. Among these safe compounds, 8-epi-loganin showed the best docking score of − 8.17 kcal/mol against cyclooxygenase-2 enzyme (PDB ID: 6COX), which was almost similar to that of the standard drug, Celecoxib (− 11.11 kcal/mol). Conclusion In conclusion, L. alba can be a potent source of analgesic medicine and further modification and simulation studies are required to establish the effectiveness of 8-epi-loganin.

Evaluation of the Anti-Inflammatory and Analgesic Activities of the Total Saponins Extracted from Fermented Polygala japonica Houtt

2013 ◽  
Vol 641-642 ◽  
pp. 931-934
Author(s):  
Ming Xing Liu ◽  
Ting Zhu ◽  
She Ne Guo ◽  
Hong Da Zhu
Keyword(s):  
Experimental Study ◽  
Acetic Acid ◽  
Vascular Permeability ◽  
Analgesic Activity ◽  
Inflammatory Activity ◽  
Hot Plate ◽  
Plate Models ◽  
Anti Inflammatory ◽  
Analgesic Activities

This study was to investigate the anti-inflammatory and analgesic activities of the total saponins extracted from fermented Polygala japonica Houtt (FPH) compared with that of unfermented Polygala japonica Houtt (UFPH). The total saponins extracted from FPH and UFPH were evaluated for anti-inflammatory activity in xylene-induced ear swelling and acetic acid-induced vascular permeability models in mice, analgesic activity in acetic acid-induced writhing and hot plate models in mice. The total saponins extracted from FPH had the significant anti-inflammatory (p<0.001) and analgesic (p<0.01) activities with the doses of 6 g/kg b.w. in mice. The results of this experimental study thus strongly support the potential significant use of the total saponins extracted from FPH for pain and inflammatory.

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