PHYTONUTRIENT SCREENING BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY AND ANTI-AGING PROPERTIES OF GLYCOSMIS PENTAPHYLLA (RETZ.) DC. (SOM-CHUEN) EXTRACTS

2021 ◽  
pp. 55-58
Author(s):  
THISAKORN DUMRONGPHUTTIDECHA ◽  
WARACHATE KHOBJAI ◽  
SURACHAI TECHAEOI ◽  
KHEMJIRA JARMKOM ◽  
SURADWADEE THUNGMUNGMEE
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bovine Serum Albumin ◽  
Gas Chromatography Mass Spectrometry ◽  
Abts Assay ◽  
Chromatography Mass Spectrometry ◽  
Matrix Metalloproteinase 1 ◽  
Aging Properties ◽  
Aging Property ◽  
Deoxyribose Degradation

Objective: The aims of this study were to screen the phytonutrient constituents and investigate their anti-aging property of leaves and bark ofGlycosmis pentaphylla (Retz.) DC. (GP).Methods: GP is the medicinal plant which used as traditional medicine. Glycosmis pentaphylla leaves (GPL) and Glycosmis pentaphylla bark (GPB) ofGP were extracted with ethanol. The gas chromatography–mass spectrometry was used for phytonutrients analysis. The anti-aging properties wereperformed using ABTS assay, deoxyribose degradation assay, bovine serum albumin (BSA)-fructose model, and matrix metalloproteinase 1 (MMP-1)inhibitory activity.Results: The important phytonutrients of GPL were α-tocopherol, linolenic acid, squalene, stigmasterol, and β-amyrin and for GPB were α-tocopherol,phytol, campesterol, stigmasterol, dictamine, and γ-sitosterol. The percentage inhibition of GPL and GPB extracts at various concentrations wasbetween 16.57–76.05 and 20.66-78.81 by ABTS assay, 68.24–90.06 and 73.83–96.64% by deoxyribose degradation assay, 4.24–99.98 and 54.81–99.94 by BSA-fructose model, and 6.31–81.55 and 1.06–74.45 by MMP-1 inhibitory activity, respectively.Conclusion: Leaves and bark extracts of GP (Retz.) DC. are good sources of important phytonutrients and have the potential in anti-aging property.

CHARACTERISATION OF DISSOLVED ORGANIC CARBON (DOC) LEACHED FROM LEAVES IN WATER

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Markus Heryanto Langsa
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Total Organic Carbon ◽  
Gas Chromatography Mass Spectrometry ◽  
Pyrolysis Gas ◽  
Pyrolysis Gas Chromatography ◽  
Chromatography Mass Spectrometry ◽  
Pinus Radiate

<p>Penelitian ini bertujuan untuk menentukan senyawa organik khususnya organic karbon terlarut (DOC) dari dua spesies daun tumbuhan (<em>wandoo eucalyptus </em>and <em>pinus radiate, conifer</em>) yang larut dalam air selama periode 5 bulan leaching eksperimen. Kecepatan melarutnya senyawa organic ditentukan secara kuantitatif dan kualitatif menggunakan kombinasi dari beberapa teknik diantaranya Total Organic Carbon (TOC) analyser, Ultraviolet-Visible (UV-VIS) spektrokopi dan pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS).</p><p>Hasil analisis DOC dan UV menunjukkan peningkatan yang tajam dari kelarutan senyawa organic di awal periode pengamatan yang selanjutnya berkurang seiring dengan waktu secara eksponensial. Jumlah relatif senyawa organic yang terlarut tergantung pada luas permukaan, aktifitas mikrobiologi dan jenis sampel tumbuhan (segar atau kering) yang digunakan. Fluktuasi profil DOC dan UV<sub>254</sub> disebabkan oleh aktifitas mikrobiologi. Diperoleh bahwa daun kering lebih mudah terdegradasi menghasilkan senyawa organic dalam air dibandingkan dengan daun segar. Hasil pyrolysis secara umum menunjukkan bahwa senyawa hidrokarbon aromatic dan fenol (dan turunannya) lebih banyak ditemukan pada residue sampel setelah proses leaching kemungkinan karena adanya senyawa lignin atau aktifitas humifikasi mikrobiologi membuktikan bahwa senyawa-senyawa tersebut merupakan komponen penting dalam proses karakterisasi DOC.</p>

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