scholarly journals Effect of Enhancement of Two Beef Muscles with Phosphate or Alternative Functional Ingredients on the Eating Quality of US Beef

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
B. I. Mills ◽  
N. C. Hardcastle ◽  
A. J. Garmyn ◽  
M. F. Miller

ObjectivesConsumers are increasingly searching for more natural and healthier foods that avoid ingredients like phosphates (“clean label”). The objective of this study was to determine the effects of enhancement ingredients and quality grade on the eating quality of longissimus lumborum and semitendinosus.Materials and MethodsStrip loins (n = 36) and eye of rounds (n = 31) were collected from beef carcasses to equally represent USDA Prime, Average Choice, and Select quality grades at a commercial packing facility in Omaha, NE. Subprimals were shipped under refrigeration (0 to 2°C) to the Texas Tech University for processing. Subprimals were trimmed of all accessory muscles, external fat, and connective tissue, leaving longissimus lumborum (LL) and semitendinosus (ST). Each subprimal was equally portioned into 6 sections. One section served as a non-enhanced control (CON), while the remaining 5 sections were injected with 112% of green weight with water, salt, and either sodium tripolyphosphate (STP), native potato starch (NPS), sodium carbonate (SC), sodium bicarbonate (SB), or beef flavoring (BF). Sections were cut into steak pieces (5 × 5 × 2.5-cm thick) and frozen at 40 d postmortem. Steak pieces were cooked to a targeted medium degree of doneness on a clamshell grill using a fixed time cooking schedule. Each sample was portioned and served warm to 2 consumer panelists. Panelists (n = 1380) rated each sample for tenderness, juiciness, flavor, and overall liking on an anchored 100-mm line scale. During a session, panelists evaluated 6 samples representing each treatment combination, arranged in a predetermined, balanced order. Data were analyzed using PROC GLIMMIX (SAS) with fixed effects of muscle, enhancement, quality grade, and their interactions (α = 0.05).ResultsNo two-way or three-way interactions were detected for any palatability trait (P > 0.05). Enhancement ingredients influenced tenderness, juiciness, flavor, and overall liking (P < 0.01), regardless of muscle or quality grade. Samples from SC and SB had greater (P < 0.05) tenderness scores than samples enhanced with any other ingredient, except PS. Meanwhile, CON samples were the least tender, and STP was scored lowest for tenderness of the enhanced treatments (P < 0.05). Samples enhanced with BF, SC, and SB were rated juicier than STP and all enhanced samples were rated juicier than CON (P < 0.05). Samples enhanced with BF, SC, SB, and NPS were all similarly rated with greater flavor and overall liking than STP (P < 0.05), which was intermediate, and CON had the lowest flavor and overall liking compared to all other treatments (P < 0.05). Quality grade also affected tenderness, juiciness, flavor and overall liking (P < 0.05). Prime samples received the greatest ratings for all traits, over Average Choice, which was intermediate, and Select samples were scored lowest for all palatability traits. Lastly, muscle influenced all palatability traits (P < 0.01). Longissimus lumborum samples were more tender, juicier, more flavorful, and liked more than semitendinosus samples (P < 0.01).ConclusionResults showed consumers liked alternative functional ingredients over enhancement with phosphate and non-enhanced beef. This shows clean label ingredients are not only effective in increasing palatability but had superior eating quality over enhancement with phosphate.

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
N. C. Hardcastle ◽  
A. J. Garmyn ◽  
M. F. Miller

ObjectivesImported meat products are commonly used in the value-addition sector of the US meat industry. Non-meat ingredients, such as sodium tripolyphosphate (STP), are often introduced into imported subprimals by the processor to mitigate potential palatability issues. Although STP can positively affect palatability attributes, its use in meat products can be concerning to some consumers. Our objectives were to determine the effects of enhancement with phosphate or alternative functional ingredients on the palatability of three imported Australian beef subprimals.Materials and MethodsRibeye rolls, strip loins, and eye of rounds were collected from carcasses (N = 69) at two commercial abattoirs in Australia. Subprimals were shipped under vacuum in a commercial refrigerated vessel at 0 to 2°C to the USA, where they were transported to Texas Tech University for processing. External fat, connective tissue, and accessory muscles were removed from subprimals, leaving the longissimus thoracics (LT), longissimus lumborum (LL), and the semitendinosus (ST). Muscles were then portioned into six equal sections. One section served as a non-enhanced control (CON), while the remaining five were injected to 112% of green weight with water, salt, and 1 of 5 ingredients: STP, sodium bicarbonate (SBC), sodium carbonate (SC), native potato starch (PS), or beef flavoring (BF). Sections were cut into steak pieces (5 × 5 × 2.5-cm thick) and frozen at 90 d postmortem. Thawed samples were cooked on a clamshell grill using a fixed time cooking schedule targeting a medium degree of doneness, cut into 2 equal portions, and served warm to 2 consumers. Panelists (n = 1380) evaluated each sample for tenderness, juiciness, flavor and overall liking on anchored 100-mm lines scales. Each consumer evaluated 6 test samples from the treatment combinations arranged in a predetermined, balanced order. Sensory data were analyzed using the GLIMMIX procedure of SAS using muscle, ingredient, and their interaction as fixed effects and abattoir as a random effect (α = 0.05).ResultsNo interactions were detected between muscle and ingredient (P ≥ 0.44); however, both muscle and ingredient affected (P < 0.01) consumer sensory ratings. The LL and LT similarly (P > 0.05) scored more tender, with greater flavor and overall liking (P < 0.05) than the ST. The LT was juicier than LL, which was intermediate, and ST was the least juicy (P < 0.05). Samples that were not enhanced were scored lower (P < 0.05) for all measured palatability attributes compared to all other treatments, except STP and CON had similar tenderness scores (P > 0.05). Of the injected samples, STP resulted in lower (P < 0.05) consumer sensory scores than all other treatments. Consumers rated SBC, SC, and PS as the most tender samples (P < 0.05). Samples enhanced with SBC and SC were juicier (P < 0.05) than all other treatments except PS. The flavor of SC was liked more (P < 0.05) than all other treatments except SBC and PS. Samples from SBC, SC, and PS had greater (P < 0.05) overall liking than STP and CON, but PS and BF were similar (P > 0.05).ConclusionUltimately, these results revealed that several alternative functional ingredients can be used to improve palatability scores of imported Australian beef while generating eating quality outcomes that are similar or superior to injection with STP.


2005 ◽  
Vol 45 (5) ◽  
pp. 499 ◽  
Author(s):  
D. L. Hopkins ◽  
P. J. Walker ◽  
J. M. Thompson ◽  
D. W. Pethick

The effect of type of lambs and hoggets on eating quality was evaluated using the M. longissimus lumborum (LL) and the M. biceps femoris (BF) from 210 animals. The animals comprised 7 types as follows: Poll Dorset or White Suffolk × (Border Leicester × Merino) (second cross unweaned lambs, mixed sex, 3–5 months of age); Poll Dorset × (Border Leicester × Merino) (second cross weaned lambs, mixed sex, 9–12 months of age); Border Leicester × Merino (BLM; first cross weaned lambs, mixed sex, 9 months of age); Poll Dorset or White Suffolk × Merino (PDM; first cross weaned lambs, wethers, 12 months of age); Merino × Merino (weaned wether lambs, 9–12 months of age); Border Leicester × Merino (BLM; first cross hoggets, mixed sex, 20 months of age); and Poll Dorset or White Suffolk × Merino (PDM; first cross hoggets, wethers, 16 months of age). The animals were sourced from a number of different properties in New South Wales (NSW) (n = 120) and Victoria (n = 90). Each carcass was subjected to high voltage stimulation (700 V) within 1 h of slaughter. All meat was aged for 5 days before freezing and was subsequently tested by consumers on a 0–100 scale for a number of attributes after cooking using a grilling procedure. The LL from sucker lambs had the lightest colour (highest L* values) with the hoggets having the darkest colour in the NSW group with differences less apparent in the Victorian group. Muscle from first cross and Merino weaned lambs had consistently higher pH levels across the 3 muscles tested (LL; M. semimembranosus; M. semitendinosus) for the NSW group with no differences detected in the Victorian group. LL meat from second cross sucker and first cross (BLM and PDM) weaned lambs had the highest overall liking score, with that from second cross weaned lambs, BLM hoggets and Merino lambs scored as intermediate, while meat from the PDM hogget group had the lowest overall liking score. A similar outcome was found for the other sensory attributes with differences between types being lower for juiciness compared with overall liking scores but they were of a similar ranking. For the BF there was some re-ranking of the overall liking scores compared with the LL, with meat from suckers and first cross PDM lambs and hoggets being intermediate in score while second cross weaned lambs and first cross BLM lambs had the highest scores. The BF from hoggets (BLM) and Merino lambs were given the lowest scores. The lower juiciness score for sucker BF contributed to this change in ranking compared with the LL.


2005 ◽  
Vol 45 (5) ◽  
pp. 483 ◽  
Author(s):  
A. B. Pleasants ◽  
J.M. Thompson ◽  
D.W. Pethick

A probabilistic model of consumer perception of sheep meat eating quality is constructed on the basis of a sensory score derived from consumer evaluation of tenderness, juiciness, flavour and overall liking. The model includes consideration of the variability of the sensory score within a mob, the probability that meat with a given sensory score will be perceived by the consumer as correctly classified, and the distribution of the average sensory score within an eating quality grade. Based on this information the model can calculate the frequency with which sheep meat given a sensory score that will fail to meet the consumer expectation of the allocated eating quality grade. The model provides a basis for integrating the information gathered on sheep meat eating quality to formulate a method of classification and to quantify the reliability of this classification. This information can be applied in a variety of ways to evaluate the efficacy, financial or otherwise, of a retailer adopting a given sheep meat eating quality classification based on the nominated eating quality score.


1990 ◽  
Vol 50 (2) ◽  
pp. 291-299 ◽  
Author(s):  
E. Dransfield ◽  
G. R. Nute ◽  
B. W. Hogg ◽  
B. R. Walters

ABSTRACTCarcass and meat composition and eating quality of m. longissimus lumborum (LI) and leg joints were compared in entire male (ram), castrated male (wether) and female (ewe) Dorset Down-cross and Suffolk-cross lambs at about 20 weeks old. Rams grew faster than wethers and ewes particularly in the earlier maturing Suffolk crosses. Carcass weights ranged from 13 to 24 kg and at the mean carcass weight of 17·7 kg ram carcasses yielded larger shoulder joints. Ram carcasses were assessed visually leaner than those from the other sexes with similar conformation scores. Dorset Down carcasses tended to be fatter than Suffolk carcasses and the leanest carcasses were from Suffolk rams. Intramuscular fatness in LI was similar in all sexes. Intramuscular collagen contents were higher in ram LI than in other sexes. There were no differences in protein content, pH or colour of raw or roast LI. In assessments of eating quality of LI by triangle tests, male and female assessors differentiated twin rams and wethers equally. Category scaling showed LI from ewes to be slightly tougher than those from rams and wethers. Consumer evaluation of leg joints did not detect any differences in odour due to sex and ram meat was assessed better than that from wethers or ewes.


2020 ◽  
Vol 33 (8) ◽  
pp. 1339-1351
Author(s):  
Seung-Hoon Jwa ◽  
Yong-An Kim ◽  
Van-Ba Hoa ◽  
In-Ho Hwang

Objective: It is well recognized that beef cuts from a low quality grade are usually associated with tougher, drier and less flavorful. Thus, the present study aimed at investigating the combined effects of postmortem ageing and sous vide (SV) cooking followed by oven roasting or blowtorching on the eating quality of low quality grade Hanwoo beef striploins.Methods: Hanwoo beef striploins (quality grade 3) obtained from 36 month-old Hanwoo steers were used, and the samples were chiller aged for 0 and 14 d at 4°C. After ageing, the samples were prepared into 2.5-cm steaks which were then SV cooked at 55°C for 5 h and then raised to 60°C for 1 h, and thereafter the SV-cooked the steaks were further roasted in oven for 20 min (SV+OV) or blowtorched (SV+TC) for 2 min. The cooked samples were analyzed for microbiological quality, browning index, Wanrner-Bratzler shear force (WBSF), aroma flavor compounds and sensory properties.Results: The SV cooking significantly reduced the WBSF values in beef samples (p<0.05). Blowtorching after SV cooking led to a browner surface of the beef steaks (p<0.05). The samples treated with SV+OV or SV+TC exhibited higher levels of Maillard reaction-derived aroma flavor compounds such as; pyrazines and sulfur-containing compounds compared to those just SV cooked. More especially, the SV+OV- or SV+TC- treated samples presented significantly higher flavor and overall acceptability scores compared to those just SV cooked (p<0.05). Ageing beef for 14 d significantly improved the tenderness by reducing the WBSF and increasing the tenderness scores.Conclusion: Thus, the combination of postmortem ageing and SV cooking followed by additional treatments (blowtorching or oven roasting) could be used to improve the eating quality especially tenderness and flavor as well as overall acceptability of low grade Hanwoo beef.


Author(s):  
Z Pietrasik ◽  
Phyllis J Shand

Abstract Several muscles from mature beef carcasses have been identified as failing to provide adequate tenderness required for a satisfactory consumer eating experience. Postmortem processing strategies can help improve the tenderness and subsequent eating quality of mature beef muscles. The current study was undertaken to investigate the impact of processing strategies (blade tenderization [BT], pre-tumbling [PT], and moisture enhancement [ME]), alone and in combination, on processing yield and eating quality-related parameters of selected loin and hip muscles (gluteus medius [GM], longissimus lumborum [LL], semimembranosus [SM], and biceps femoris [BF]) from youthful and mature beef cattle. Results indicate that muscles from mature beef were inherently less tender (P &lt; 0.05), but some tenderization procedures produced meat that was similar in tenderness to that of youthful cattle. Of the different tenderization strategies evaluated, BT followed by ME (injection of a salt/phosphate solution) was the most effective strategy for improving (P &lt; 0.05) tenderness of tougher muscle cuts such as BF and SM. Moisture enhancement alone improved tenderness (P &lt; 0.05) and juiciness (P &lt; 0.01) of SM, GM and LL, but with the exception of samples tenderized prior to injection, was not effective (P &gt; 0.05) in BF muscles. No additional tenderization of GM and LL samples was observed (P &gt; 0.05) by combining PT or BT with ME. Combining PT or BT with ME; however, was effective (P &lt; 0.05) to control the increased purge loss observed following ME treatment in SM and LL muscles. Pre-tumbling as a single treatment was ineffective (P &gt; 0.05) in all of the muscles, and only treatments that included BT were sufficient to affect an increase (P &lt; 0.05) in tenderness of BF.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
B. Hawryluk ◽  
P. Ramos ◽  
D. Johnson ◽  
M. Elzo ◽  
C. Carr ◽  
...  

ObjectivesHeat shock proteins (HSP) are biomarkers of stress and perform chaperoning functions to fold, unfold, and refold proteins after heat stress. Brahman are more heat tolerant than Angus, while Angus beef has been associated with greater palatability than Brahman. The objectives were to determine if HSP content in the longissimus lumborum differs between Angus and Brahman and examine how HSP content relates to the eating quality of beef.Materials and MethodsAngus and Brahman steers (n = 12 per breed) were finished during summer in Florida and harvested at approximately 17 mo of age. Samples of longissimus lumborum were collected at 1 h after exsanguination and were immediately immersed in liquid nitrogen. Samples were then pulverized, diluted in extraction buffer, and homogenized. The protein samples were assayed to assess protein concentration and subsequently diluted to equal concentrations for loading into acrylamide gels. Proteins were separated by gel electrophoresis, and western blotting was used to evaluate content of aβ-crystallin, HSP27, HSP60, HSP70, and HSP90. Target bands were detected and quantified using LI-COR Odyssey and target signal was normalized to total protein stain. Tenderness was evaluated in 14d-aged steaks using Warner-Bratzler shear force (WBSF) and a trained sensory panel. Data were analyzed using one-way ANOVA and Pearson correlations were conducted for content of HSPs and objective and subjective tenderness.ResultsHSP27, HSP60, and HSP70 did not differ between breeds (P > 0.05); however, HSP90 and aβ-crystallin were greater (P = 0.005) in the longissimus lumborum of Angus compared to Brahman. Even though WBSF did not differ (P = 0.29) between breeds, breed affected (P < 0.0001) sensory tenderness. Content of aβ-crystallin was associated with sensory tenderness (r2 = 0.52, P = 0.0098).ConclusionLongissimus dorsi from the Angus were contained more aβ-crystallin and HSP90 than Brahman. Elevated concentrations of both aβ-crystallin and HSP90 could be breed related or may have been influenced by the season they were harvested. While WBSF was not affected by breed, panelists rated Angus steaks as more tender after aging for 14d. Content of aβ-crystallin is associated with tenderness; however further work is necessary to determine if this small HSP affects proteolysis.


2019 ◽  
Vol 59 (2) ◽  
pp. 384 ◽  
Author(s):  
D. T. Packer ◽  
G. H. Geesink ◽  
R. Polkinghorne ◽  
J. M. Thompson ◽  
A. J. Ball

The Meat Standards Australia beef-grading model applies a variable adjustment for different cuts of hormonal growth promotant (HGP)-treated carcasses, but does not differentiate between different HGP types. Using 300 non-implanted Bos indicus–Bos taurus composite steers, an experiment was conducted to compare the effects of an oestradiol only (OES) and a combination trenbolone acetate and oestradiol (TBA+OES) implant with non-implanted animals (CON) fed a concentrate ration for 73 days before slaughter, on eating quality of the mm. longissimus lumborum (LL) and gluteus medius (GM) muscles. Sensory and objective LL and GM samples were aged for either 5 or 35 days before freezing at −20°C. Carcass weights from each group were significantly (P &lt; 0.05) different. Corrected for carcass weight, HGP treatment had a significant effect on hump height, ossification score, marble score, P8 fat depth and eye-muscle area. The TBA+OES treatment resulted in significantly (P &lt; 0.05) tougher meat than the OES and CON treatments as assessed by shear force, although this difference was reduced with aging. Sensory scores (tenderness, juiciness, like flavour, overall liking and a composite MQ4 score) confirmed a negative HGP treatment effect, whereby TBA+OES was significantly lower than the CON and OES treatments after 5 days of aging, and these differences were reduced through aging. TBA+OES had a greater impact on sensory scores in the LL when compared to the GM. Both HGP treatments increased calpastatin activity, and the TBA+OES treatment was significantly (P &lt; 0.05) different from the CON and OES treatments. It was concluded that OES and TBA+OES implants have different impacts on meat eating-quality measurements, which could have important implications for the Australian and international beef industry.


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