Immunohistochemical comparison of cyclin D1 expression in amelobelastoma, odontogenic keratocyst and radicular cyst

The odontogenic keratocyst (OKC) is a benign intraosseous cyst of the odontogenic origin, characterized by an aggressive behavior with a relatively high recurrence rate.OKC is mostly presented at the 2nd and 3rd decades and predominantly affect males. It affects mandible more than maxilla and is frequently found at the angle of mandible. OKC can clinically and radiographically mimic other benign and less aggressive lesions. It is therefore important to differentiate them from other cysts and tumors, for a proper treatment and reduction of recurrences.We report the case of a 19-year-old woman who presented with a swelling in left mandible. The clinicaland radiographicfeatures were in favor of a radicular cyst.Histological examination, however, contradicted this diagnosis, indicating a case of OKC.

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Immunohistochemical expression of Cyclooxygenase 2 reflects the proliferative activity in the epithelium of odontogenic lesions

Dentistry 3000 ◽

10.5195/d3000.2022.205 ◽

2022 ◽

Vol 10 (1) ◽

Author(s):

Vani Verma ◽

Chetana Chandrashekar ◽

Raghu Radhakrishnan ◽

Monica Charlotte Solomon

Keyword(s):

Epithelial Cells ◽

Dentigerous Cyst ◽

Cyclooxygenase 2 ◽

Odontogenic Keratocyst ◽

Odontogenic Cysts ◽

Radicular Cyst ◽

Chi Square ◽

Ki 67 ◽

Odontogenic Epithelium ◽

Cox 2

Purpose: Odontogenic cysts and tumors comprise a major component of lesions of the oral and maxillofacial region. The pathogenesis of these lesions involves the interaction between the odontogenic epithelium and the ectomesenchyme. However, the clinical behavior of these biological entities is unpredictable. The aim of this study was to evaluate the role of Cyclooxygenase 2 (COX-2) in the pathogenesis and prognostication of odontogenic lesions.Material and method: : In this study formalin-fixed paraffin-embedded tissue section of Odontogenic Keratocyst (n=10) Dentigerous cyst (n=10), Radicular cyst (n=10) and unicystic ameloblastoma (n=10) were immunohistochemically stained with COX-2 (NCL2-COX-2- 4H12) and with Ki 67 (Ki-67 GM001) using standard staining protocols. The cytoplasmic expression of COX-2 in all the lesions was semi-quantitatively assessed. The pattern of expression of COX-2 among the different odontogenic lesions was statistical analyzed using the ANOVA test and the chi-square test.Results: All the 40 odontogenic lesions that were evaluated expressed COX-2 immunohistochemically. A high number of odontogenic epithelial cells expressed COX-2 in most of the odontogenic keratocyst, radicular cyst and unicystic ameloblastomas. The expression of COX-2 was significantly (p=0.036) higher in Unicystic Ameloblastomas and Radicular cyst compared to that of Odontogenic Keratocyst and the dentigerous cyst.Conclusion: The recognition that expression of COX-2 by odontogenic epithelial cells may indeed shed a new light on the biological mechanisms involved in the development of these benign yet aggressive lesions of the jaws. An insight into the molecular interactions occurring in the odontogenic epithelium will aid in better management of these lesions.

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