scholarly journals Bioactive molecules for regenerative pulp capping

2021 ◽  
Vol 42 ◽  
pp. 415-437
Author(s):  
LLE Whitehouse ◽  
◽  
NH Thomson ◽  
T Do ◽  
GA Feichtinger
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Dental Pulp ◽  
Specific Area ◽  
Bioactive Molecules ◽  
Current Evidence ◽  
Pulp Capping ◽  
Biomimetic Scaffolds ◽  
Dental Pulp Capping ◽  
Biomimetic Scaffold

Since the discovery of bioactive molecules sequestered in dentine, researchers have been exploring ways to harness their activities for dental regeneration. One specific area, discussed in this review, is that of dental-pulp capping. Dental-pulp caps are placed when the dental pulp is exposed due to decay or trauma in an attempt to enhance tertiary dentine deposition. Several materials are used for dental-pulp capping; however, natural biomimetic scaffolds may offer advantages over manufactured materials such as improved aesthetic, biocompatibility and success rate. The present review discusses and appraises the current evidence surrounding biomimetic dental-pulp capping, with a focus on bioactive molecules sequestered in dentine. Molecules covered most extensively in the literature include transforming growth factors (TGF-βs, specifically TGF-β1) and bone morphogenetic proteins (BMPs, specifically BMP-2 and BMP-7). Further studies would need to explore the synergistic use of multiple peptides together with the development of a tailored scaffold carrier. The roles of some of the molecules identified in dentine need to be explored before they can be considered as potential bioactive molecules in a biomimetic scaffold for dental-pulp capping. Future in vivo work needs to consider the inflammatory environment of the dental pulp in pulpal exposures and compare pulp-capping materials.

scholarly journals Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: a comparative analysis on the Umbilical Cord and Dental Pulp derived Stem/ Stromal Cells secretome

2019 ◽  
Author(s):  
AR Caseiro ◽  
SS Pedrosa ◽  
G Ivanova ◽  
MV Branquinho ◽  
A Almeida ◽  
...  
Keyword(s):  
Growth Factor ◽  
Umbilical Cord ◽  
Stromal Cells ◽  
Dental Pulp ◽  
Tube Formation ◽  
Bioactive Molecules ◽  
Resonance Spectroscopy ◽  
Conditioning Period ◽  
Chemotactic Protein

AbstractMesenchymal Stem/ Stromal Cells assume a supporting role to the intrinsic mechanisms of tissue regeneration, a feature mostly assigned to the contents of their secretome. A comparative study on the metabolomic and bioactive molecules/factors content of the secretome of Mesenchymal Stem/ Stromal Cells derived from two expanding sources: the umbilical cord stroma and the dental pulp is presented and discussed. The metabolic profile (Nuclear Magnetic Resonance Spectroscopy) evidenced some differences in the metabolite dynamics through the conditioning period, particularly on the glucose metabolism. Despite, overall similar profiles are suggested. More prominent differences are highlighted for the bioactive factors (Multiplexing Laser Bear Analysis), in which Follistatin, Growth Regulates Protein, Hepatocyte Growth Factor, Interleukin-8 and Monocyte Chemotactic Protein-1 dominate in Umbilical Cord Mesenchymal Stem/ Stromal Cells secretion, while in Dental Pulp Stem/ Stromal Cells the Vascular Endothelial Growth Factor-A and Follistatin are more evident. The distinct secretory cocktail did not result in significantly different effects on endothelial cell populations dynamics including proliferation, migration, tube formation capacity and in vivo angiogenesis, or in chemotaxis for both Mesenchymal Stem/ Stromal Cells populations.

scholarly journals Materiais Utilizados para Capeamento Pulpar Direto: uma Revisão da Literatura

2018 ◽  
Vol 19 (5) ◽  
pp. 209
Author(s):  
C M A Silva ◽  
L S Paiva ◽  
D V Oliveira ◽  
B O Nobre ◽  
T A D Mendes ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Biocompatible Materials ◽  
Pulp Capping ◽  
Dental Pulp Capping

O objetivo deste estudo é avaliar as propriedades físico-químicas e biológicas dos principais materiais indicados para capeamento pulpar direto. Revisou-se a literatura, nos bancos de dados Pubmed, Scopus e Scielo, entre 2007 e 2017, buscando artigos em português e em inglês, utilizando os descritores combinados: “Dental Pulp Capping”, “Biocompatible Materials” e “Calcium Hydroxide” como estratégia de busca. Foram obtidos 55 artigos em que 21 estavam em repetição e foram excluídos, dos demais 6 eram revisão de literatura, 3 eram ensaios clínicos e 25 estudos laboratoriais. Através de uma leitura crítica dos títulos e dos resumos, foram selecionados 10 artigos segundo sua relevância para o estudo. Os materiais mais relatados foram o hidróxido de cálcio, o agregado trióxido mineral (MTA) e o silicato tricálcio (biodentine®). O primeiro contém propriedades antibacterianas e biocompatibilidade, entretanto possui a sua alta solubilidade como desvantagem. O segundo apresenta resultados mais previsíveis que o primeiro na formação da barreira dentinária, melhor biocompatibilidade e boa capacidade seladora. O terceiro mostra maior formação de dentina terciária, promovendo um bom selamento marginal e mostra a menor solubilidade entre eles. Assim, os materiais MTA e biodentine mostram-se materiais viáveis ao tradicional hidróxido de cálcio.Palavras-chave: Dental Pulp Capping. Biocompatible Materials. Calcium Hydroxide. 

scholarly journals Evaluation of Resin-Based Material Containing Copaiba Oleoresin (Copaifera Reticulata Ducke): Biological Effects on the Human Dental Pulp Stem Cells

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 972
Author(s):  
Roberta Souza D’Almeida Couto ◽  
Maria Fernanda Setubal Destro Rodrigues ◽  
Leila Soares Ferreira ◽  
Ivana Márcia Alves Diniz ◽  
Fernando de Sá Silva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Biological Effects ◽  
Dental Pulp Stem Cells ◽  
Nodule Formation ◽  
Alizarin Red ◽  
Pulp Capping ◽  
Hsp 27 ◽  
Dental Pulp Capping ◽  
Human Dental Pulp

The purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay) and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 + COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility, differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of Ca(OH)2.

scholarly journals A Review of Current Regenerative Medicine Strategies that Utilize Nanotechnology to Treat Cartilage Damage

2016 ◽  
Vol 10 (1) ◽  
pp. 862-876 ◽  
Author(s):  
R. Kumar ◽  
M. Griffin ◽  
P.E. Butler
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cartilage Damage ◽  
Bioactive Molecules ◽  
Cartilage Defects ◽  
Biomimetic Scaffolds ◽  
Cartilage Reconstruction ◽  
Biomaterial Science

Background: Cartilage is an important tissue found in a variety of anatomical locations. Damage to cartilage is particularly detrimental, owing to its intrinsically poor healing capacity. Current reconstructive options for cartilage repair are limited, and alternative approaches are required. Biomaterial science and Tissue engineering are multidisciplinary areas of research that integrate biological and engineering principles for the purpose of restoring premorbid tissue function. Biomaterial science traditionally focuses on the replacement of diseased or damaged tissue with implants. Conversely, tissue engineering utilizes porous biomimetic scaffolds, containing cells and bioactive molecules, to regenerate functional tissue. However, both paradigms feature several disadvantages. Faced with the increasing clinical burden of cartilage defects, attention has shifted towards the incorporation of Nanotechnology into these areas of regenerative medicine. Methods: Searches were conducted on Pubmed using the terms “cartilage”, “reconstruction”, “nanotechnology”, “nanomaterials”, “tissue engineering” and “biomaterials”. Abstracts were examined to identify articles of relevance, and further papers were obtained from the citations within. Results: The content of 96 articles was ultimately reviewed. The literature yielded no studies that have progressed beyond in vitro and in vivo experimentation. Several limitations to the use of nanomaterials to reconstruct damaged cartilage were identified in both the tissue engineering and biomaterial fields. Conclusion: Nanomaterials have unique physicochemical properties that interact with biological systems in novel ways, potentially opening new avenues for the advancement of constructs used to repair cartilage. However, research into these technologies is in its infancy, and clinical translation remains elusive.

scholarly journals Evaluation of pH changes produced by dental pulp capping materials: An in vitro study

2017 ◽  
Vol 54 (2) ◽  
pp. 161-165
Author(s):  
Elzbieta Luczaj-Cepowicz ◽  
Grazyna Marczuk-Kolada ◽  
Malgorzata Pawinska ◽  
Marta Obidzinska
Keyword(s):  
Dental Pulp ◽  
In Vitro Study ◽  
Vitro Study ◽  
Pulp Capping ◽  
Ph Changes ◽  
Dental Pulp Capping

Otoliths/Bacterial Cellulose Nanocomposite as a Potential Dental Pulp Capping Biomaterial in Canine Model

2015 ◽  
Vol 120 (2) ◽  
pp. e105 ◽  
Author(s):  
JULIANA BATISTA MELO DA FONTE ◽  
DAISY PEREIRA VALIDO ◽  
LAURO XAVIER FILHO ◽  
LIGIA MARIA MANZINE COSTA ◽  
GABRIEL MOLINA DE OLYVEIRA ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Dental Pulp ◽  
Canine Model ◽  
Pulp Capping ◽  
Dental Pulp Capping

NOS Evaluations in Human Dental Pulp-Capping with MTA and Calcium-Hydroxide

2007 ◽  
Vol 20 (1_suppl) ◽  
pp. 27-32 ◽  
Author(s):  
C. D'arcangelo ◽  
F. Di Nardo-Di Maio ◽  
C. Patrono ◽  
S. Caputi
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Human Dental Pulp
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