scholarly journals Biochemical Evaluation of Some Liverworts Pigments and Phenolics

2021 ◽  
Vol 11 (4-S) ◽  
pp. 78-80
Author(s):  
K.M. Khalkar ◽  
V.B. Kadam
Keyword(s):  
Similar Type ◽  
Polyphenol Content ◽  
Biochemical Evaluation

Three genera viz. Riccia, Plagiochasma and Targionia were studied biochemically. It was found that amount of total chlorophylls and polyphenol content is more in Targionia as compared to Riccia and Plagiochasma Flavonoids are also studied adopting chromatography technique and when the spots viewed under UV were found to be of similar type in Riccia, and Plagiochasma, whereas Targionia showed the present of only one spot ,identical to the second spot isolated in Riccia, and Plagiochasma Keywords: Biochemical, Riccia, Plagiochasma, Targionia, pigments, phenolics.

En face dual fluorescence staining of fatty streaks allows observation of initiation and progression of atherosclerotic lesions

1995 ◽  
Vol 53 ◽  
pp. 864-865
Author(s):  
Anne M. Klinkner ◽  
Crystal R. Waites ◽  
Peter J. Bugelski ◽  
William D. Kerns
Keyword(s):  
Fluorescent Dyes ◽  
Nile Red ◽  
Dimethyl Formamide ◽  
High Cholesterol Diet ◽  
Methods Development ◽  
Atherosclerotic Lesions ◽  
En Face ◽  
Biochemical Evaluation ◽  
Stock Solutions ◽  
Dual Staining

A primary effort in the understanding of the progression of atherosclerotic disease has been methods development for visualization of the atherosclerotic plaque. We introduce a new method for the qualitative analysis of lipids in atherosclerotic fatty streaks which also retains those lipids for biochemical evaluation. An original aspect of the process is the ability to view an entire fatty streak en face, selectively stained for specific lipid classes within the lesion.New Zealand white rabbits were fed a high cholesterol diet(0.15%-0.3% for 14 wks). The aorta was removed and fixed in Carson's phosphate buffered formaldehyde followed by dual staining in the fluorescent dyes Nile red and filipin. Stock solutions of nile red(0.5mg/ml acetone) and filipin(2.5mg/ml dimethyl formamide) were prepared and kept at -20°C; all subsequent steps were at RT. 0.5cm × 1.0cm pieces of aorta were trimmed and adventitia removed. The pieces were then washed 3×15 min in PBS w/o CaMg, soaked in Nile red(NR)/filipin(Fl) stain(100(il NR stock + 200μl Fl stock in 10 ml PBS for 30 min, washed in PBS 3×30 min, rinsed with distilled water, mounted(Crystal Mount, Biomedia) and coverslipped and viewed by fluorescence microscopy.

Biochemical evaluation of Mitragyna ciliata Aubrev & Pellegr (Rubiaceae)

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
HA Ogbunugafor ◽  
VI Okochi ◽  
J Okpuzor ◽  
OS Odesanmi
Keyword(s):  
Biochemical Evaluation

Biochemical Evaluation to Mesenchymal Stem Cells Therapy of Renal Tubulointerstitial Injury

2011 ◽  
Vol 3 (10) ◽  
pp. 1-5
Author(s):  
Zahran F Zahran F ◽  
◽  
El-Ghareb M El-Ghareb M ◽  
Nashwa Barakat ◽  
El-Naggar I El-Naggar I
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tubulointerstitial Injury ◽  
Stem Cells Therapy ◽  
Biochemical Evaluation

Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Fraction ◽  
Rosa Multiflora ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Water Fraction ◽  
Total Polyphenol Content ◽  
Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

Sign in / Sign up

Export Citation Format

Share Document