Kemiripan genetik klon karet (Hevea brasiliensis Muell. Arg.) berdasarkan metode Amplified Fragment Length  Polymorphisms (AFLP) Genetic similarity of rubber clones (Hevea brasiliensis Muell. Arg) based on Amplified Fragment Length Polymorphisms (AFLP) method

Summary Genetic similarity among ten rubber clones originating from the Wickham collection was studied by Amplified Fragment Length Polymorphism (AFLP) markers. These clones have different levels of resistance to Corynespora cassiicola, one of the major pathogens in rubber plantations. The information resulted from this study will be used to determine resistant and susceptible clones which will be used in expression study of the genes encoding plant resistance to C. cassiicola. Genetic similarity values of clones were calculated from all AFLP markers and used to produce a dendrogram using Unweight Pair-Group Method Arithmetic (UPGMA) based on Numerical Taxonomy and Multivariate System (NTSYS) version 1.8 pc. A total of 481 fragments were detected by using ten pairs of selective AFLP primers, and 233 fragments (48,4 %) of them were polymorphic. The results clearly demonstrated that genetic background of these ten clones were 85.5% similar. At 88.0% similarity level, the clones could be divided into three clusters. Genetic similarity of IRR 100 (resistant clone) with RRIC 103, PPN 2444 and IAN 873 (susceptible clones) was 90.5, 89.5 and 89.0% respectively, while genetic similarity of other three resistant clones (AVROS 2037, PR 255 and BPM 1) to those susceptible clones was 88.0%. The lowest genetic similarity (85.5%) was found between RRIC 100 (resistant clone) and those three susceptible clones. By considering the distribution and the source of clones, AVROS 2037 (resistant) and PPN 2444 (susceptible) clones which have 88.0% genetic similarity will finally be selected for the expression study of the genes. Kemiripan genetik sepuluh klon karet yang berasal dari koleksi Wickham dipelajari dengan menggunakan marka Amplified Fragment Length Polymorphism (AFLP). Kesepuluh klon tersebut memiliki tingkat resistensi berbeda terhadap Corynespora cassiicola, salah satu cendawan patogen penting pada daun tanaman karet. Informasi yang diperoleh dalam penelitian ini akan digunakan untuk menetapkan klon resisten dan klon rentan untuk digunakan dalam mempelajari ekspresi gen yang menyandikan ketahanan tanaman karet terhadap C. cassiicola. Nilai kemiripan genetik kesepuluh klon karet dihitung berdasarkan semua marka AFLP yang diperoleh dan selanjutnya digunakan untuk. membuat dendrogram dengan menggunakan Unweight Pair-Group Method Arithmetic (UPGMA) berdasarkan Numerical Taxonomy and Multivariate System (NTSYS) version 1.8 pc. Dengan menggunakan 10 pasang primer AFLP selektif diperoleh sebanyak 481 fragmen DNA, S 2037, PR 255 and BPM 1) to those susceptible clones was 88.0%. The lowest genetic similarity (85.5%) was found between RRIC 100 (resistant clone) and those three susceptible clones. By considering the distribution and the source of clones, AVROS 2037 (resistant) and PPN 2444 (susceptible) clones which have 88.0% genetic similarity will finally be selected for the expression study of the genes. 233 fragmen (48,4 %) di antaranya polimorfik Dendrogram dengan nyata menunjukkan bahwa 85,5% latar belakang genetik kesepuluh klon karet tersebut adalah sama, dan pada tingkat 88,0% kesepuluh klon terpisah dalam tiga kelompok. Kemiripan genetik klon IRR 100 (resisten) dengan klon rentan RRIC 103, PPN 2444 dan IAN 873 masing-masing adalah 90,5, 89,5 dan 89,0%, sedangkan kemiripan genetik tiga klon resisten lainnya (AVROS 2037, PR 255 dan BPM 1) dengan ketiga klon rentan yang sama adalah 88,0%. Kemiripan genetik terendah (85,5%) terdapat antara klon RRIC 100 (resisten) dengan ketiga klon rentan tersebut. Dengan mempertimbangkan distribusi penyebaran klon dan asal klon maka klon resisten AVROS 2037 dan klon rentan PPN 2444 yang memiliki kemiripan genetik 88,0% akan dipilih untuk digunakan dalam studi ekspresi gen tanaman karet. acerun:yes'> Kesepuluh klon tersebut memiliki tingkat resistensi berbeda terhadap Corynespora cassiicola, salah satu cendawan patogen penting pada daun tanaman karet. Informasi yang diperoleh dalam penelitian ini akan digunakan untuk menetapkan klon resisten dan klon rentan untuk digunakan dalam mempelajari ekspresi gen yang menyandikan ketahanan tanaman karet terhadap C. cassiicola. Nilai kemiripan genetik kesepuluh klon karet dihitung berdasarkan semua marka AFLP yang diperoleh dan selanjutnya digunakan untuk. membuat dendrogram dengan menggunakan Unweight Pair-Group Method Arithmetic (UPGMA) berdasarkan Numerical Taxonomy and Multivariate System(NTSYS) version 1.8 pc. Dengan menggunakan 10 pasang primer AFLP selektif diperoleh sebanyak 481 fragmen DNA, S 2037, PR 255 and BPM 1) to those susceptible clones was 88.0%. The lowest genetic similarity (85.5%) was found between RRIC 100 (resistant clone) and those three susceptible clones. By considering the distribution and the source of clones, AVROS 2037 (resistant) and PPN 2444 (susceptible) clones which have 88.0% genetic similarity will finally be selected for the expression study of the genes.

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Kemiripan genetik klon karet (Hevea brasiliensis Muell. Arg.) berdasarkan metode Amplified Fragment Length Polymorphisms (AFLP) Genetic similarity of rubber clones (Hevea brasiliensis Muell. Arg) based on Amplified Fragment Length Polymorphisms (AFLP) method

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v71i1.180 ◽

2016 ◽

Vol 71 (1) ◽

Author(s):

. NURHAIMI-HARIS ◽

Hajrial ASWIDINNOOR ASWIDINNOOR ◽

Nurita TORUAN-MATHIUS ◽

Agus PURWANTARA

Keyword(s):

Fragment Length ◽

Genetic Similarity ◽

Numerical Taxonomy ◽

Group Method ◽

Corynespora Cassiicola ◽

Resistant Clone ◽

Expression Study ◽

Multivariate System ◽

Pair Group ◽

Length Polymorphisms

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Genetic Diversity Analysis of Aluminum-toxicity Tolerant and Sensitive Soybean Genotypes Assessed with Microsattelite Markers

Jurnal AgroBiogen ◽

10.21082/jbio.v5n1.2009.p1-6 ◽

2009 ◽

Vol 5 (1) ◽

pp. 1

Author(s):

I Made Tasma ◽

Ahmad Warsun

Keyword(s):

Genetic Diversity ◽

Numerical Taxonomy ◽

Aluminum Toxicity ◽

Group Method ◽

Diversity Analysis ◽

Multivariate System ◽

Genetic Diversity Analysis ◽

Pair Group ◽

Soybean Genotypes

Analisis Diversitas Genetik Genotipe Kedelai Toleran dan Peka Keracunan Aluminium Menggunakan Marka Mikrosatelit. I Made Tasma dan Ahmad Warsun. Persilangan dua genotipe kedelai dengan jarak genetik jauh menghasilkan progeni dengan polimorfisme tinggi pada banyak lokus yang memfasilitasi keberhasilan program pemuliaan dan pemetaan karakter agronomi penting kedelai. Tujuan penelitian ini untuk mengetahui diversitas genetik genotipe kedelai toleran dan peka keracunan aluminium (Al), informasi diversitas alel dan tingkat polimorfisme marka SSR dari genotipe kedelai yang diuji, menentukan genotipe dengan jarak genetik jauh sebagai tetua dalam pembentukan populasi pemetaan karakter toleran Al, dan informasi diversitas genetik dalam pemilihan tetua untuk program pemuliaan kedelai toleran keracunan Al. Dua puluh empat genotipe kedelai toleran dan peka keracunan Al dianalisis menggunakan 15 marka SSR. Marka SSR lokasinya menyebar pada 14 kromosom kedelai. Dendrogram dikonstruksi menggunakan Unweighted Pair-Group Method Arithmatic (UPGMA) melalui program Numerical Taxonomy and Multivariate System (NTSYS) versi 2.1-pc. Diversitas genetik antara dua genotipe kedelai berkisar antara 2-33,2%. Pada diversitas 33,2% uji klaster UPGMA membagi genotipe menjadi 2 kelompok masing-masing terdiri dari 19 dan 5 genotipe untuk kelompok 1 dan 2. Jumlah alel SSR total 81dengan rata-rata jumlah alel per lokus SSR 4,4 dan rata-rata tingkat polimorfisme 0,55. Menggunakan diversitas tertinggi 33,2% dua genotipe paling peka Al (B3293 dan B3442) dari kelompok 1 dan dua genotipe paling toleran Al (B3462 dan B3851) dari kelompok 2 dipilih untuk membentuk populasi pemetaan karakter toleran Al. Berdasarkan nilai diversitas genetik tertinggi 33,2% banyak kemungkinan kombinasi persilangan dapat dilakukan antara genotipe toleran Al untuk pemuliaan kedelai toleran Al.

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Phenetic Characterization of Plum Cultivars by High Multiplex Ratio Markers: Amplified Fragment Length Polymorphisms and Inter-simple Sequence Repeats

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.126.1.72 ◽

2001 ◽

Vol 126 (1) ◽

pp. 72-77 ◽

Cited By ~ 19

Author(s):

Luís Goulão ◽

Luisa Monte-Corvo ◽

Cristina M. Oliveira

Keyword(s):

Fragment Length ◽

Simple Sequence Repeats ◽

Group Method ◽

Similarity Coefficients ◽

Pair Group ◽

Multiple Loci ◽

Inter Simple Sequence Repeats ◽

Length Polymorphisms ◽

Amplified Fragment Length Polymorphisms ◽

Simple Sequence

Variability of commercial plum (Prunus L. sp.) cultivars is unknown since breeding often involves intercrossing hybrids with several species but has been based on a low number of parents. Molecular markers like amplified fragment length polymorphisms (AFLP) and inter-simple sequence repeats (ISSR), which sample multiple loci simultaneously, have become increasingly popular, and were used to characterize 24 diploid and four hexaploid cultivars of plum. Seven AFLP and six ISSR primers were used, and resulted in amplification of 379 and 270 products, respectively. Unweighted pair-group method with arithmetic averages (UPGMA) dendrograms, based on similarity coefficients, reflected a clear separation between diploid and hexaploid plums. Among diploid plums, two pairs of cultivars were relatively distinct from the rest, namely `Golden Japan' and `Methley' and `Ozark Premier' and `Songold'. Furthermore, several cultivars were grouped together both with AFLP and ISSR analysis: 1) `Ambra', `Red Beaut', and `Black Beaut', 2) `Black Diamond' and `Royal Diamond', 3) `June Rose', `Santa Rosa', and `Royal Red', and iv) `Freedom', `Larry Ann', and `Queen Rosa'. Although the phenetic classification obtained by the two methods were similar (r = 0.73, for the diploid group), ISSR had a higher reproducibility and percentage of polymorphisms (87.4% vs. 62.8%) than AFLP. Methodological aspects of both markers systems are discussed. Results obtained suggest that the AFLP and ISSR approaches are valuable tools for identification of specific genotypes and analysis of phenetic relationships in plum.

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Genetic structure and molecular variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) collected in maize and cotton fields in Brazil

Bulletin of Entomological Research ◽

10.1017/s0007485307004944 ◽

2007 ◽

Vol 97 (3) ◽

pp. 225-231 ◽

Cited By ~ 36

Author(s):

S. Martinelli ◽

P.L. Clark ◽

M.I. Zucchi ◽

M.C. Silva-Filho ◽

J.E. Foster ◽

...

Keyword(s):

Spodoptera Frugiperda ◽

Genetic Similarity ◽

Crop Protection ◽

Fall Armyworm ◽

Arithmetic Mean ◽

Group Method ◽

Pair Group ◽

Length Polymorphisms ◽

Amplified Fragment Length Polymorphisms ◽

Lepidoptera Noctuidae

AbstractThe purpose of this research was to evaluate the genetic similarity and structure of the fall armyworm, Spodoptera frugiperda (J.E. Smith), populations associated with maize and cotton crops in Brazil using amplified fragment length polymorphisms. Mean genetic similarity among populations was 0.45. The unweighted pair group method with arithmetic mean analysis dendrograms did not separate populations of S. frugiperda into clusters related to the host plant in which the insects were collected. No genetic variation was observed among maize and cotton populations of S. frugiperda, suggesting that the same populations are injuring both crops in Brazil. This research validates the need for stewardship of crop-protection methods for managing S. frugiperda to reduce the incidence of pesticide resistance, due to the spatial and temporal overlapping of maize and cotton crops in some regions in Brazil.

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Genetic analysis of a heritage variety collection

Plant Genetic Resources ◽

10.1017/s1479262118000448 ◽

2018 ◽

Vol 17 (03) ◽

pp. 232-244 ◽

Cited By ~ 2

Author(s):

J. M. Preston ◽

B. V. Ford-Lloyd ◽

L. M. J. Smith ◽

R. Sherman ◽

N. Munro ◽

...

Keyword(s):

Genetic Analysis ◽

Group Method ◽

Niche Markets ◽

Principal Coordinates Analysis ◽

Pair Group ◽

Landrace Diversity ◽

Principal Coordinates ◽

Length Polymorphisms ◽

Seed Saving ◽

History Of

AbstractLandraces (including heritage varieties) are an important agrobiodiversity resource offering considerable value as a buffer against crop failures, as a crop for niche markets, and as a source of diversity for crop genetic improvement activities underpinning future food security. Home gardens are reservoirs of landrace diversity, but some of the accessions held in them are vulnerable or threatened with extinction. Those associated with seed saving networks have added security, for example, ca. 800 varieties are stored in the Heritage Seed Library (HSL) of Garden Organic, UK. In this study, Amplified Fragment Length Polymorphisms-based genetic analysis of accessions held in the HSL was used to (a) demonstrate the range of diversity in the collection, (b) characterize accessions to aid collection management and (c) promote broader use of the collection. In total, 171 accessions were included from six crops: Vicia faba L., Pisum sativum L., Daucus carota L., Cucumis sativus L., Lactuca sativa L. and Brassica oleracea L. var. acephala (DC.) Metzq. Average expected heterozygosity ranged from 0.18 to 0.28 in D. carota; 0.02–0.18 in P. sativum; 0.05–0.18 in L. sativa; 0.15–0.26 in B. oleracea var. acephala; 0.15–0.37 in C. sativus and 0.07–0.36 in V. faba. Genetic diversity and Fst values generally reflected the breeding system and cultivation history of the different crops. Comparisons of the diversity found in heritage varieties with that found in commercial varieties did not show a consistent pattern. Principal coordinates analysis and Unweighted Pair Group Method with Arithmetic Mean cluster analysis were used to identify four potential duplicate accession pairs.

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Assessment of Genetic Relationships among Philodendron Cultivars Using AFLP Markers

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.129.5.0690 ◽

2004 ◽

Vol 129 (5) ◽

pp. 690-697 ◽

Cited By ~ 4

Author(s):

Pachanoor S. Devanand ◽

Jianjun Chen ◽

Richard J. Henny ◽

Chih-Cheng T. Chao

Keyword(s):

Genetic Similarity ◽

Near Infrared ◽

Genetic Relationships ◽

Aflp Markers ◽

Group Method ◽

Limited Information ◽

Similarity Coefficients ◽

Arithmetic Average ◽

Ornamental Foliage Plants ◽

Pair Group

Philodendrons (Philodendron Schott) are among the most popular tropical ornamental foliage plants used for interior decoration. However, limited information is available on the genetic relationships among popular Philodendron species and cultivars. This study analyzed genetic similarity of 43 cultivars across 15 species using amplified fragment length polymorphism (AFLP) markers with near infrared fluorescence labeled primers. Forty-eight EcoR I + 2/Mse I + 3 primer set combinations were screened, from which six primer sets were selected and used in this investigation. Each selected primer set generated 96 to 130 scorable fragments. A total of 664 AFLP fragments were detected, of which 424 (64%) were polymorphic. All cultivars were clearly differentiated by their AFLP fingerprints, and the relationships were analyzed using the unweighted pair-group method of arithmetic average cluster analysis (UPGMA) and principal coordinated analysis (PCA). The 43 cultivars were divided into five clusters. Cluster I comprises eight cultivars with arborescent growth style. Cluster II has only one cultivar, `Goeldii'. There are 16 cultivars in cluster III, and most of them are self-heading interspecific hybrids originated from R.H. McColley's breeding program in Apopka, Fla. Cluster IV contains 13 cultivars that exhibit semi-vining growth style. Cluster V has five cultivars that are true vining in morphology, and they have lowest genetic similarity with philodendrons in other clusters. Cultivated philodendrons are generally genetically diverse except the self-heading hybrids in cluster III that were mainly developed using self-heading and semi-vining species as parents. Seven hybrid cultivars have Jaccard's similarity coefficients of 0.88 or higher, suggesting that future hybrid development needs to select parents with diverse genetic backgrounds.

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Application of ITAP-PCR Techniques to Assess the Genetic Variability of Selected Cultivars of Winter Triticale (× Triticosecale Wittmack)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47311494 ◽

2019 ◽

Vol 47 (3) ◽

pp. 947-953

Author(s):

Izabela SZUĆKO ◽

Anna MĄDRACH

Keyword(s):

Genetic Variability ◽

Genetic Similarity ◽

Group Method ◽

Fast Method ◽

Winter Triticale ◽

Pair Group ◽

Cereal Plant ◽

Seed Market ◽

Polymorphism Rate ◽

Triticosecale Wittmack

The increasing use of triticale (× Triticosecale Wittmack) indicates that its position on the seed market is constantly strengthening; therefore, the research on its genetic variability is necessary to improve breeding process of new cultivars. The aim of the study was to assess the possibility of using the ITAP-PCR technique to analyse the genetic similarity of nine cultivars of winter triticale cultivated in Poland. Primers designed on the basis of 6 DNA transposon sequences commonly found in cereal plant genomes were used for the study. The average polymorphism rate in the genotypes used in the study was determined as 95.24%; in total, 75 bands were obtained, of which 73 were polymorphic. The PIC value ranged between 0.27 and 0.44, and was highest for the Hamlet primer. The lowest PIC value was observed for the Mutator primer. The average DI value was 0.34, MI - 4.08, AEI - 12.17 and IPI - 4.40. SI ranged from 36.7% to 1.7%. A dendrogram was created according to the unweighted pair group method with arithmetic mean (UPGMA), which in terms of genetic similarity divided the analysed winter triticale cultivars into two main similarity groups.We confirmed that ITAP technique of transposon-based marker is efficient and fast method to detect genetic variability between different winter triticale cultivars. In addition, the presence of analyzed transposon families in hexaploid triticale has not been studied earlier.

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Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

Canadian Journal of Plant Science ◽

10.4141/cjps07088 ◽

2008 ◽

Vol 88 (2) ◽

pp. 313-322 ◽

Cited By ~ 22

Author(s):

S. C. Debnath ◽

S. Khanizadeh ◽

A. R. Jamieson ◽

C. Kempler

Keyword(s):

Genetic Diversity ◽

Simple Sequence Repeat ◽

Genetic Similarity ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Group Method ◽

Sequence Repeat ◽

Breeding Lines ◽

Pair Group ◽

Simple Sequence

The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria H ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs. Key words: Fragaria × ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity

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Amplified fragment length polymorphism and mitochondrial sequence data detect genetic differentiation and relationships in endangered southwestern U.S.A. ambersnails (Oxyloma spp.)

Canadian Journal of Zoology ◽

10.1139/z00-119 ◽

2000 ◽

Vol 78 (10) ◽

pp. 1845-1854 ◽

Cited By ~ 5

Author(s):

Mark P Miller ◽

Larry E Stevens ◽

Joseph D Busch ◽

Jeff A Sorensen ◽

Paul Keim

Keyword(s):

Length Polymorphism ◽

Fragment Length ◽

Population Genetic ◽

Fragment Length Polymorphism ◽

Sequence Data ◽

Amplified Fragment Length Polymorphism ◽

Group Method ◽

Exact Test ◽

Additional Information ◽

Pair Group

The Kanab ambersnail (Oxyloma haydeni kanabensis) is a federally endangered mollusc currently known to reside in two locations in the southwestern U.S.A. To determine the extent of within- and between-population genetic variation of this taxon, the amplified fragment length polymorphism (AFLP) technique was used to generate 110 genetic markers among individuals sampled from the two Kanab ambersnail populations and from the only two known southwestern populations of the Niobrara ambersnail (Oxyloma haydeni haydeni) in Utah and northern Arizona. Additional information was obtained from sequence data of cytochrome b and cytochrome oxidase I gene fragments. Results suggest high levels of differentiation among populations, as evidenced through the application of UPGMA (unweighted pair-group method with arthimetic averaging) clustering, F statistics, and Fisher's exact test. Various levels of within-population genetic diversity were observed among populations. Expected heterozygosities ranged from 0.239 to 0.086 under a model assuming Hardy-Weinberg genotypic proportions and ranged from 0.205 to 0.061 under an obligate-selfing completely homozygous model. Results from cluster analyses showed that one Kanab ambersnail population and one Niobrara ambersnail population were more similar than the two Kanab ambersnail populations studied (supported by >80% of bootstrap replicates). These findings were further supported through the phylogenetic analysis of both mito chondrial gene fragments. The data suggest that taxonomic designations need revision, an act that will likely affect the protected status of some of the populations.

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Keragaman genetik klon-klon karet (Hevea brasiliensis Muell. Arg) yang resisten dan rentan terhadap Corynespora casiicola berdasarkan penanda RAPD dan AFLP Genetic variation of rubber ( Hevea brasiliensis Muell. Arg) clones resistance and susceptible to Corynespora cassiicola using RAPD and AFLP markers

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v70i2.127 ◽

2016 ◽

Vol 70 (2) ◽

Author(s):

Nurita TORUAN-MATHIUS ◽

Z LALU ◽

. SOEDARSONO ◽

Hajrial ASWIDINNOOR

Keyword(s):

Hevea Brasiliensis ◽

Genetic Similarity ◽

Pcr Amplification ◽

Aflp Markers ◽

Group Method ◽

Aflp Analysis ◽

Pair Group ◽

Young Leaves ◽

Cluster A ◽

Susceptible Group

SummaryCorynespora leaf fall disease (CFLD) caused by the fungus Corynespora casiicola is one of the most important diseases of Hevea brasiliensis.CFLD was reported to cause serious damage on rubber productivity, and the disease has became more apparent in the recent years. The objectives of this study were (i) to analyze genetic similarities among several rubber clones resistance and susceptible to CFLD based on RAPD and AFLP markers, (ii) to compare the effectiveness of RAPD and AFLP markers. DNA genomic was extracted from young leaves of RRIM600, GT1, PB260, RRIC100, BPM1 (belongs to resistance group), PPN2058, PPN2444, and PPN2447 (belongs to susceptible group). Data were analyzed with NTSYS-pc program version 2.10, and a dendogram was created by cluster analysis using the unweighted pair group method on the basis of arithmetic averages (UPGMA). The results show that marker index AFLP (3.57) is higher than RAPD (1.02), it means that AFLP is more effective compared to RAPD. The average of genetic similarity AFLP (0.63) lower than RAPD (0.67) it means that AFLP is more discriminative than RAPD. Dendogram based on AFLP and RAPD were the best with at 0.65 level of genetic similarity cluster divided into two cluster A and B. Cluster A with a sub group A1 consisted of RRIC100, PPN2058 and PPN244 are belongs to resistance group), and sub group A2 consisted of (RRIM600, GT1, BPM1 and PB 260 are belongs to susceptible group), while cluster B only PPN2447 is belong to susceptible group. AFLP analysis show that one AFLP band of 110 bp resulting from PCR amplification using E-ACA/M-CAG (E-ACA/M-CAG110) primer pairs present in resistance clones, but absent in the susceptible clones. Meanwhile, application of 50 random primers decamer in RAPD analysis did not showed the specific band for either one of the group. It is concluded that AFLP marker analysis using EACA/M-CAG primer pair have a potential to differentiate resistance and the susceptible rubber clones to Corynespora. For the confirmation of the results more resistance and susceptible clones are needed for further test. RingkasanPenyakit gugur daun Corynespora (PDGC) yang disebabkan oleh patogen Corynespora asiicola, merupakan salah satu penyakit penting pada tanaman karet (Hevea brasiliensis). PGDC menyebabkan penurunan yang cukup serius terhadap produktivitas tanaman karet. Tujuan penelitian ini adalah untuk (i) mengidentifikasi kesamaan genetik antar beberapa klon yang tergolong tahan dan rentan dengan marka RAPD dan AFLP, dan (ii) mempelajari efektivitas kedua marka tersebut. DNA genomik diekstraksi dari daun muda klon RRIM600, GT1, PB260, BPM1, RRIC100 (tergolong resisten), PPN2058, PPN2444, dan PPN2447 (tergolong rentan ). Data dianalisis dengan NTSYS-pc program versi 2.10. Dendogram dibuat dengan analisis pengelompokan menurut metode Unweighted Pair Group berbasis Arithmetic Avarages (UPGMA). Hasil yang diperoleh menunjukkan bahwa marka indeks AFLP (3,57) lebih tinggi daripada RAPD (1,02), sehingga AFLP lebih efektif dibandingkan dengan RAPD. Rata-rata perkiraan kesamaan genetik AFLP (0,63) sedikit lebih rendah dari RAPD (0,67) sehingga AFLP relatif lebih diskriminatif daripada RAPD. Dendogram berdasarkan integrasi AFLP dan RAPD adalah yang paling baik, dimana pada rata-rata perkiraan kesamaan genetik (0,65) terbentuk dua kelompok yaitu A dan B. Kelompok A terdiri atas sub sub kelompok A1 yang beranggotakan (RRIC100, PPN2058 dan PPN244 yang tergolong resisten), dan sub group A2 yang beranggotakan (RRIM600, GT1, BPM1 dan PB 260 yang tergolong rentan) Sedang kelompok B beranggotakan hanya PN2447 yang tergolong rentan. Analisis AFLP menghasilkan satu pita AFLP dengan menggunakan pasangan primer EACA/M-CG (E-ACA/M-CAG110 ) secara konsisten diperoleh dari klon karet yang resisten, namun tidak ditemukan pada klon yang rentan. Sementara itu, aplikasi 50 primer acak dekamer dalam analisis RAPD tidak menghasilkan pita spesifik untuk kedua kelompok yang diuji. Disimpulkan bahwa analisis AFLP menggunakan pasangan primer EACA/M-CAG berpotensi untuk membedakan klon karet yang resisten dan rentan terhadap Corynespora. Untuk mengkorfirmasi hasil yang diperoleh, perlu dilakukan pengujian terhadap klon-klon yang resisten dalam jumlah yang lebih banyak

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