scholarly journals Application of ITAP-PCR Techniques to Assess the Genetic Variability of Selected Cultivars of Winter Triticale (× Triticosecale Wittmack)

2019 ◽  
Vol 47 (3) ◽  
pp. 947-953
Author(s):  
Izabela SZUĆKO ◽  
Anna MĄDRACH
Keyword(s):  
Genetic Variability ◽  
Genetic Similarity ◽  
Group Method ◽  
Fast Method ◽  
Winter Triticale ◽  
Pair Group ◽  
Cereal Plant ◽  
Seed Market ◽  
Polymorphism Rate ◽  
Triticosecale Wittmack

The increasing use of triticale (× Triticosecale Wittmack) indicates that its position on the seed market is constantly strengthening; therefore, the research on its genetic variability is necessary to improve breeding process of new cultivars. The aim of the study was to assess the possibility of using the ITAP-PCR technique to analyse the genetic similarity of nine cultivars of winter triticale cultivated in Poland. Primers designed on the basis of 6 DNA transposon sequences commonly found in cereal plant genomes were used for the study. The average polymorphism rate in the genotypes used in the study was determined as 95.24%; in total, 75 bands were obtained, of which 73 were polymorphic. The PIC value ranged between 0.27 and 0.44, and was highest for the Hamlet primer. The lowest PIC value was observed for the Mutator primer. The average DI value was 0.34, MI - 4.08, AEI - 12.17 and IPI - 4.40. SI ranged from 36.7% to 1.7%. A dendrogram was created according to the unweighted pair group method with arithmetic mean (UPGMA), which in terms of genetic similarity divided the analysed winter triticale cultivars into two main similarity groups.We confirmed that ITAP technique of transposon-based marker is efficient and fast method to detect genetic variability between different winter triticale cultivars. In addition, the presence of analyzed transposon families in hexaploid triticale has not been studied earlier.

scholarly journals GENETIC VARIABILITY OF Rottboellia cochinchinensis POPULATIONS IN SUGARCANE FIELDS

2016 ◽  
Vol 34 (3) ◽  
pp. 475-484 ◽  
Author(s):  
A.R. SCHIAVETTO ◽  
D. PERECIN ◽  
L.R. PINTO ◽  
C.A.M. AZANIA ◽  
F.S. ZERA ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Genetic Similarity ◽  
Sao Paulo ◽  
The State ◽  
Group Method ◽  
Jaccard Coefficient ◽  
São Paulo ◽  
Pair Group ◽  
Analysis System ◽  
Rottboellia Cochinchinensis

ABSTRACT The hypothesis assumed was the existence of biotypes within populations, which has been the cause of difficulties in itchgrass control by farmers. For that, the genetic variability of three populations of Rottboellia cochinchinensis in sugarcane fields in the state of São Paulo was investigated by using the Amplified Fragment Length Polymorphism (AFLP) technique. Six primers were used to obtain molecular characterization data. AFLP gels were analyzed based on marker presence (1) and absence (0). Using NTSYs (Numerical Taxonomy and Multivariate Analysis System) software, the genetic similarity was calculated by the Jaccard coefficient and, from that, a dendrogram was built through the UPGMA (Unweighted Pair Group Method Arithmetic averages) method, besides determining the isopolymorphic marks. The average genetic similarities seen in the region was 0.742 for Igarapava, 0.793 for Mococa and 0.808 for Piracicaba. Between regions it was 0.730 (Igarapava vs Mococa), 0.735 (Mococa vs Piracicaba) and 0.694 (Igarapava vs Piracicaba). In line with the dendrogram, it is possible to detect the formation of two groups, one with 8 plants from Igarapava and Mococa and the other with 21 plants from Igarapava, Mococa and Piracicaba, as well as the presence of 1 discriminant individual from Piracicaba. It can be concluded that the genetic similarity among itchgrass populations from the state of São Paulo was high (72%), which denotes that the difficulties in chemical management are not only due to different biotypes but also due to other characteristics linked to tolerance of the species to herbicides. However, biotype existence cannot be discarded because of the polymorphic marks generating 22% average genetic variability.

scholarly journals Detection of DNA and Ploidy Variation within Vegetatively Propagated Zoysiagrass Cultivars

2014 ◽  
Vol 139 (5) ◽  
pp. 547-552 ◽  
Author(s):  
Karen R. Harris-Shultz ◽  
Susana Milla-Lewis ◽  
Aaron J. Patton ◽  
Kevin Kenworthy ◽  
Ambika Chandra ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Reference Sample ◽  
Warm Season ◽  
The United States ◽  
Group Method ◽  
Group Iii ◽  
Climatic Regions ◽  
Pair Group ◽  
Group I ◽  
Two Samples

Zoysiagrass (Zoysia sp.) is used as a warm-season turfgrass for lawns, parks, and golf courses in the warm, humid and transitional climatic regions of the United States. Zoysiagrass is an allotetraploid species (2n = 4x = 40) and some cultivars are known to easily self- and cross-pollinate. Previous studies showed that genetic variability in the clonal cultivars Emerald and Diamond was likely the result of contamination (seed production or mechanical transfer) or mislabeling. To determine the extent of genetic variability of vegetatively propagated zoysiagrass cultivars, samples were collected from six commercially available zoysiagrass cultivars (Diamond, Emerald, Empire, JaMur, Meyer, Zeon) from five states (Arkansas, Florida, Georgia, North Carolina, Texas). Two of the newest cultivar releases (Geo and Atlantic) were to serve as outgroups. Where available, one sample from university research plots and two samples from sod farms were collected for each cultivar per state. Forty zoysiagrass simple sequence repeat (SSR) markers and flow cytometry were used to compare genetic and ploidy variation of each collected sample to a reference sample. Seventy-five samples were genotyped and an unweighted pair group method with arithmetic mean clustering revealed four groups. Group I (Z. japonica) included samples of ‘Meyer’ and Empire11 (‘Empire’ sample at location #11), Group II (Z. japonica × Z. pacifica) included samples of ‘Emerald’ and ‘Geo’, Group III (Z. matrella) included samples of ‘Diamond’ and ‘Zeon’, and Group IV (Z. japonica) consisted of samples from ‘Empire’, ‘JaMur’, ‘Atlantic’, and Meyer3 (‘Meyer’ at sample location #3). Samples of ‘Empire’, ‘Atlantic’, and ‘JaMur’ were indistinguishable with the markers used. Four samples were found to have alleles different from the respective reference cultivar, including two samples of ‘Meyer’, one sample of ‘Empire’, and one sample of ‘Emerald’. Three of these samples were from Texas and one of these samples was from Florida. Three of the four samples that were different from the reference cultivar were university samples. In addition, one sample, Empire11, was found to be an octoploid (2n = 8x = 80). For those samples that had a fingerprint different from the reference cultivar, contamination, selfing, and/or hybridization with other zoysiagrasses may have occurred.

scholarly journals Assessment of Genetic Relationships among Philodendron Cultivars Using AFLP Markers

2004 ◽  
Vol 129 (5) ◽  
pp. 690-697 ◽  
Author(s):  
Pachanoor S. Devanand ◽  
Jianjun Chen ◽  
Richard J. Henny ◽  
Chih-Cheng T. Chao
Keyword(s):  
Genetic Similarity ◽  
Near Infrared ◽  
Genetic Relationships ◽  
Aflp Markers ◽  
Group Method ◽  
Limited Information ◽  
Similarity Coefficients ◽  
Arithmetic Average ◽  
Ornamental Foliage Plants ◽  
Pair Group

Philodendrons (Philodendron Schott) are among the most popular tropical ornamental foliage plants used for interior decoration. However, limited information is available on the genetic relationships among popular Philodendron species and cultivars. This study analyzed genetic similarity of 43 cultivars across 15 species using amplified fragment length polymorphism (AFLP) markers with near infrared fluorescence labeled primers. Forty-eight EcoR I + 2/Mse I + 3 primer set combinations were screened, from which six primer sets were selected and used in this investigation. Each selected primer set generated 96 to 130 scorable fragments. A total of 664 AFLP fragments were detected, of which 424 (64%) were polymorphic. All cultivars were clearly differentiated by their AFLP fingerprints, and the relationships were analyzed using the unweighted pair-group method of arithmetic average cluster analysis (UPGMA) and principal coordinated analysis (PCA). The 43 cultivars were divided into five clusters. Cluster I comprises eight cultivars with arborescent growth style. Cluster II has only one cultivar, `Goeldii'. There are 16 cultivars in cluster III, and most of them are self-heading interspecific hybrids originated from R.H. McColley's breeding program in Apopka, Fla. Cluster IV contains 13 cultivars that exhibit semi-vining growth style. Cluster V has five cultivars that are true vining in morphology, and they have lowest genetic similarity with philodendrons in other clusters. Cultivated philodendrons are generally genetically diverse except the self-heading hybrids in cluster III that were mainly developed using self-heading and semi-vining species as parents. Seven hybrid cultivars have Jaccard's similarity coefficients of 0.88 or higher, suggesting that future hybrid development needs to select parents with diverse genetic backgrounds.

scholarly journals Genetic variability in natural populations of Zeyheria montana mart. from the Brazilian Cerrado

2007 ◽  
Vol 64 (4) ◽  
pp. 409-415 ◽  
Author(s):  
Bianca Waléria Bertoni ◽  
Spartaco Astolfi Filho ◽  
Ernani Ronie Martins ◽  
Carlos Ferreira Damião Filho ◽  
Suzelei de Castro França ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Natural Populations ◽  
Mantel Test ◽  
Scientific Data ◽  
Group Method ◽  
Polymorphism Analysis ◽  
Popular Medicine ◽  
Pair Group ◽  
Optimized Protocol ◽  
Geographical Distances

Zeyheria montana, an endemic species of the Bignoniaceae family from the Brazilian Cerrado's known for its anti-cancer properties, is widely used as imuno stimulant in the popular medicine and its therapeutic activity must be validated by scientific data. The objective of this work was to evaluate the genetic variability of eight plant populations collected within the state of São Paulo, Brazil, via Random Amplification of Polymorphic DNA (RAPD) used as molecular markers. After an optimized protocol for the amplification reaction, nine selected primers generated 105 reproducible bands, indicating up to 60% polymorphism. Analysis of molecular variance (AMOVA) revealed higher genetic variation within populations (84.03%) than among populations (15.97%). The variation values estimated by phiST (0.160) indicated moderate to high inter population structuration. Levels of similarity inter plants with genetic and geographical distances, estimated by the unweighted pair-group method analysis (UPGMA) clustering and non-metric multidimensional scaling (NMDS) ordination methods and by the Mantel test (-0.2345 p = 0.118) denoted that the structure found follows the island model, which assumes that a single population of infinite size may have initiated the existing populations of Zeyheria montana, with no spatial position correlation. Based on the obtained data, a germplasm bank from individuals representing the species variability was established. Furthermore the information here reported can be of importance to develop strategies for the conservation of Z. montana.

Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

2008 ◽  
Vol 88 (2) ◽  
pp. 313-322 ◽  
Author(s):  
S. C. Debnath ◽  
S. Khanizadeh ◽  
A. R. Jamieson ◽  
C. Kempler
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Genetic Similarity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Breeding Lines ◽  
Pair Group ◽  
Simple Sequence

The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria H ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs. Key words: Fragaria × ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity

scholarly journals Keragaman genetik klon-klon karet (Hevea brasiliensis Muell. Arg) yang resisten dan rentan terhadap Corynespora casiicola berdasarkan penanda RAPD dan AFLP Genetic variation of rubber ( Hevea brasiliensis Muell. Arg) clones resistance and susceptible to Corynespora cassiicola using RAPD and AFLP markers

2016 ◽  
Vol 70 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
Z LALU ◽  
. SOEDARSONO ◽  
Hajrial ASWIDINNOOR
Keyword(s):  
Hevea Brasiliensis ◽  
Genetic Similarity ◽  
Pcr Amplification ◽  
Aflp Markers ◽  
Group Method ◽  
Aflp Analysis ◽  
Pair Group ◽  
Young Leaves ◽  
Cluster A ◽  
Susceptible Group

SummaryCorynespora leaf fall disease (CFLD) caused by the fungus Corynespora casiicola is one of the most important diseases of Hevea brasiliensis.CFLD was reported to cause serious damage on rubber productivity, and the disease has became more apparent in the recent years. The objectives of this study were (i) to analyze genetic similarities among several rubber clones resistance and susceptible to CFLD based on RAPD and AFLP markers, (ii) to compare the effectiveness of RAPD and AFLP markers. DNA genomic was extracted from young leaves of RRIM600, GT1, PB260, RRIC100, BPM1 (belongs to resistance group), PPN2058, PPN2444, and PPN2447 (belongs to susceptible group). Data were analyzed with NTSYS-pc program version 2.10, and a dendogram was created by cluster analysis using the unweighted pair group method on the basis of arithmetic averages (UPGMA). The results show that marker index AFLP (3.57) is higher than RAPD (1.02), it means that AFLP is more effective compared to RAPD. The average of genetic similarity AFLP (0.63) lower than RAPD (0.67) it means that AFLP is more discriminative than RAPD. Dendogram based on AFLP and RAPD were the best with at 0.65 level of genetic similarity cluster divided into two cluster A and B. Cluster A with a sub group A1 consisted of RRIC100, PPN2058 and PPN244 are belongs to resistance group), and sub group A2 consisted of (RRIM600, GT1, BPM1 and PB 260 are belongs to susceptible group), while cluster B only PPN2447 is belong to susceptible group. AFLP analysis show that one AFLP band of 110 bp resulting from PCR amplification using E-ACA/M-CAG (E-ACA/M-CAG110) primer pairs present in resistance clones, but absent in the susceptible clones. Meanwhile, application of 50 random primers decamer in RAPD analysis did not showed the specific band for either one of the group. It is concluded that AFLP marker analysis using EACA/M-CAG primer pair have a potential to differentiate resistance and the susceptible rubber clones to Corynespora. For the confirmation of the results more resistance and susceptible clones are needed for further test. RingkasanPenyakit gugur daun Corynespora (PDGC) yang disebabkan oleh patogen Corynespora asiicola, merupakan salah satu penyakit penting pada tanaman karet (Hevea brasiliensis). PGDC menyebabkan penurunan yang cukup serius terhadap produktivitas tanaman karet. Tujuan penelitian ini adalah untuk (i) mengidentifikasi kesamaan genetik antar beberapa klon yang tergolong tahan dan rentan dengan marka RAPD dan AFLP, dan (ii) mempelajari efektivitas kedua marka tersebut. DNA genomik diekstraksi dari daun muda klon RRIM600, GT1, PB260, BPM1, RRIC100 (tergolong resisten), PPN2058, PPN2444, dan PPN2447 (tergolong rentan ). Data dianalisis dengan NTSYS-pc program versi 2.10. Dendogram dibuat dengan analisis pengelompokan menurut metode Unweighted Pair Group berbasis Arithmetic Avarages (UPGMA). Hasil yang diperoleh menunjukkan bahwa marka indeks AFLP (3,57) lebih tinggi daripada RAPD (1,02), sehingga AFLP lebih efektif dibandingkan dengan RAPD. Rata-rata perkiraan kesamaan genetik AFLP (0,63) sedikit lebih rendah dari RAPD (0,67) sehingga AFLP relatif lebih diskriminatif daripada RAPD. Dendogram berdasarkan integrasi AFLP dan RAPD adalah yang paling baik, dimana pada rata-rata perkiraan kesamaan genetik (0,65) terbentuk dua kelompok yaitu A dan B. Kelompok A terdiri atas sub sub kelompok A1 yang beranggotakan (RRIC100, PPN2058 dan PPN244 yang tergolong resisten), dan sub group A2 yang beranggotakan (RRIM600, GT1, BPM1 dan PB 260 yang tergolong rentan) Sedang kelompok B beranggotakan hanya PN2447 yang tergolong rentan. Analisis AFLP menghasilkan satu pita AFLP dengan menggunakan pasangan primer EACA/M-CG (E-ACA/M-CAG110 ) secara konsisten diperoleh dari klon karet yang resisten, namun tidak ditemukan pada klon yang rentan. Sementara itu, aplikasi 50 primer acak dekamer dalam analisis RAPD tidak menghasilkan pita spesifik untuk kedua kelompok yang diuji. Disimpulkan bahwa analisis AFLP menggunakan pasangan primer EACA/M-CAG berpotensi untuk membedakan klon karet yang resisten dan rentan terhadap Corynespora. Untuk mengkorfirmasi hasil yang diperoleh, perlu dilakukan pengujian terhadap klon-klon yang resisten dalam jumlah yang lebih banyak

scholarly journals Genetic Variability Assay of Different Natural and Hatchery Populations of Rohu (Labeo rohita) in Bangladesh

2015 ◽  
Vol 9 (1) ◽  
pp. 30-36
Author(s):  
Shikder Saiful Islam ◽  
Md. Saifuddin Shah ◽  
Foyez Ibn Shams ◽  
Md. Rayhan Ali ◽  
Md. Lifat Rahi
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Labeo Rohita ◽  
Genetic Distances ◽  
Raw Material ◽  
Group Method ◽  
Pair Group ◽  
Fish Samples ◽  
Population Genetic Variation ◽  
High Level

The level of genetic variation determines the genetic status and provides the raw material for selective improvement of a stock. Randomly amplified polymorphic DNA (RAPD) technique was used to assess the genetic variability of 7 different natural (2) and hatchery (5) populations of Indian Major Carp, Labeo rohita (Rohu) in Bangladsh. In total, 140 fish samples were collected (20 from each of the populations). Genomic DNA was extracted from the muscle tissue, and 5 different oligonucleotide primers were used which revealed 80% polymorphic DNA bands. The polymorphic loci proportions were 0.71, 0.75, 0.75, 0.85, 0.84, 0.86 and 0.89 for Ma-Fatema hatchery, Chowdhuri hatchery, Niribili hatchery, Sonali hatchery, Kapotakha hatchery, the Halda river and the Baluhor Baor populations respectively. The pair-wise population differentiation (FST) values indicated a high level of genetic variation between different populations. The Unweighted Pair Group Method of Arithmetic Mean (UPGMA) dendogram based on Nei’s genetic distances also revealed high level of inter-population genetic variation among the populations. The populations were segregated into two groups: the Halda River and Baluhar Baor hatchery in one group and Kapotakha, Ma-Fatema, Chowdhuri, Niribili and Sonali hatcheries in another group. Overall, RAPD results clearly indicate the reduced genetic quality of the hatchery seeds.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11923 International Journal of Life Sciences Vol.9(1) 2015 30-36

scholarly journals Deteksi Kestabilan Genetik Ramet Kelapa Sawit Hasil Kultur In Vitro Menggunakan SSR

2016 ◽  
Vol 44 (1) ◽  
pp. 83
Author(s):  
Yuni Fitri Cahyaningsih ◽  
Ni Made Armini Wiendi ◽  
Dan Nurita Toruan-Mathius
Keyword(s):  
Tissue Culture ◽  
Stability Analysis ◽  
Oil Palm ◽  
Genetic Stability ◽  
Genetic Similarity ◽  
Qualitative Data ◽  
Elaeis Guineensis ◽  
Group Method ◽  
Pair Group

ABSTRACT<br /><br />Commercial production of oil palm ramet requires the guarantee of high genetic stability. The objectives of this research were to determine 1) genetic diversity of ortet as source of explant, and 2) genetic stability of ramet derived from ortet propagated through tissue culture. Genetic stability analysis was done using ramet from five Tenera (D×P) oil palm ortets.As many as 20 ramets were randomly chosen from each ortet. A total of 100 ramets were used for genetic stability analysis. Genetic similarity analysis was analyzed using NTSyspc version 2.1 software with method Similarity for Qualitative Data and Unweighted Pair Group Method Aritmatic (UPGMA). The results indicated 20 SSR primer pairs were polymorphic and could form 44 alleles. As many as 80% of ramets from IS 3 ortet showed genetic similarity ranged from 97-100% to the ortet. All ramets derived from IS 10, IS 20 and IS 40 ortet had 90-100% of genetic similarity to its respective ortet. Futhermore, 95% of ramets from IS 39 ortet had 97-100% of genetic similarity to the ortet.<br /><br />Keywords: Elaeis guineensis Jacq., genetic similarity, tissue culture<br /><br />

scholarly journals Molecular Analysis of Hybrids among the Ornamental Eucalypts Eucalyptus macrocarpa, E. pyriformis, and E. youngiana

2001 ◽  
Vol 126 (3) ◽  
pp. 336-339 ◽  
Author(s):  
Kirsty Neaylon ◽  
Kate L. Delaporte ◽  
Margaret Sedgley ◽  
Graham G. Collins ◽  
John G. Conran
Keyword(s):  
Genetic Similarity ◽  
Minimum Spanning Tree ◽  
Molecular Data ◽  
Pcr Analysis ◽  
Group Method ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Mature Trees ◽  
Pair Group ◽  
Polymerase Chain

The potential for hybridization among three species of Eucalyptus L'Hér in the Series Macrocarpae, E. macrocarpa Hook (Mottlecah), E. pyriformis Turcz. (pear-fruited mallee), and E. youngiana F. Muell. (large-fruited mallee), was investigated using molecular data generated by randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) analysis. Samples of DNA from seedlings derived from controlled pollinations, and from different individuals from each species, were amplified with six different 10-mer primers. The presence or absence of RAPD fragments was used to generate a dendrogram based on genetic similarity, an ordination derived by multidimensional scaling (MDS), and a minimum spanning tree (MST) to show the relative links and dissimilarities between the individuals tested. Two clusters were identified on the unweighted pair-group method arithmetric average dendrogram. The first included all of the E. macrocarpa genotypes and all but one of the E. macrocarpa hybrids. The second included all of the E. youngiana and E. pyriformis genotypes and their hybrids. The MDS ordinations placed the hybrid seedlings between the parent species. From the 30 progeny investigated, 28 were assessed from the molecular data to be hybrids from controlled pollinations. The remaining two seedlings appeared to be derived from self-pollination. The parentage of two mature trees, thought to be natural hybrids involving the three species, was also investigated. One was confirmed as a cross between E. youngiana and E. pyriformis, but the second was less certain because of its low genetic similarity to all other individuals, and may be a hybrid involving species not included in this study.

scholarly journals Genetic Variability of 21 Tea Genotypes [Camellia sinensis (L.) O. Kuntze] Based on RAPD Markers

2018 ◽  
Vol 5 (2) ◽  
pp. 77
Author(s):  
Budi Martono ◽  
Syafaruddin Syafaruddin
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Similarity ◽  
Binary Data ◽  
Rapd Markers ◽  
Group Method ◽  
Breeding Programs ◽  
Pair Group ◽  
Monomorphic Band ◽  
And Cluster Analysis

<em>Knowing the genetic diversity in the tea germplasms collection is one of important conditions for assembling new superior varieties. Information of genetic diversity can be obtained through analysis using RAPD molecular markers. The study aimed to determine the genetic diversity of 21 tea genotypes based on RAPD markers. The research was conducted in Integrated Laboratory, Seameo Biotrop, Bogor, from July to September 2013. Genomic DNA was isolated from 21 tea genotypes leaf samples, then amplified with primer OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, and OPD 08. Electrophoresis result was converted into binary data. The genetic similarity and cluster analysis calculation was done using NTSYS-pc version 2.10. In this research, 50 polymorphic bands (94,34%) and 3 monomorphic band (5,66%) were obtained. Cluster analysis based on Nei's genetic distance using the unweighted pair-group method with arithmatic (UPGMA) divided 21 tea genotypes into two groups at a genetic similarity value of 0,48. Group 1 consisted of 20 tea genotypes, while the second group comprised only a one genotype (Sin 27). The range of genetic similarity matrix was between 28%–92%, the lowest genetic similarity (28%) was found between GMB 4 and Sin 27 genotypes, while the highest (92%) was found between AS 2 and AS 1 genotypes. The information obtained can be utilized in breeding programs with the support of agronomic characters as well as in the conservation of tea germplasm.</em>

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