THE EFFECT OF CULTURAL AND ENVIRONMENTAL CONDITIONS ON BIODEGRADATION AND BIOSURFACTANT PRODUCTION BY SERRATIA MARCESCENS UTILIZING WEATHERED DIESEL OIL

Abstract Biosurfactants are surface active substances which reduce interfacial tension and are produced or excreted at the microbial cell surface. We evaluated the biosurfactant production by Aspergillus fumigatus and Phialemonium sp. in solid state processes using fixed-bed column reactors. We evaluated two media, rice husks alone (simple support) and rice husks plus defatted rice bran (complex support), both enriched with either soy oil or diesel oil. The highest water-in-oil emulsifying activity (EAw/o) obtained was 7.36 EU g-1 produced by A. fumigatus growing on complex support enriched with soy oil and supplied with air at a rate of 60 mL g-1 h-1, while Phialemonium sp. had a maximum production of 6.11 EU g-1 using the simple support with diesel oil and an aeration rate of 120 mL g-1 h-1. The highest oilin- water emulsifying activity (EAo/w) was 12.21 EU g-1 produced by Phialemonium sp. on the complex support enriched with diesel oil and at an aeration rate of 60 mL g-1 h-1, while A. fumigatus produced a maximum EAo/w of 10.98 EU g-1 when growing on the complex support with no additional carbon source and an aeration rate of 60 mL g-1 h-1.

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Evaluation of the effect of nutrient ratios on biosurfactant production by Serratia marcescens using a Box-Behnken design

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2011.04.026 ◽

2011 ◽

Vol 86 (2) ◽

pp. 384-389 ◽

Cited By ~ 17

Author(s):

T. Roldán-Carrillo ◽

X. Martínez-García ◽

I. Zapata-Peñasco ◽

G. Castorena-Cortés ◽

J. Reyes-Avila ◽

...

Keyword(s):

Serratia Marcescens ◽

Biosurfactant Production ◽

Nutrient Ratios ◽

Box Behnken Design

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Effect of Biodiesel Concentration on Corrosion of Carbon Steel by Serratia marcescens

MATEC Web of Conferences ◽

10.1051/matecconf/201815601008 ◽

2018 ◽

Vol 156 ◽

pp. 01008 ◽

Cited By ~ 2

Author(s):

Yustina M Pusparizkita ◽

Tjandra Setiadi ◽

Ardiyan Harimawan

Keyword(s):

Carbon Steel ◽

Corrosion Rate ◽

Serratia Marcescens ◽

Corrosion Product ◽

Diesel Oil ◽

Corrosion Process ◽

Alternative Source ◽

Petroleum Reserves ◽

Growth Of Bacteria

Biodiesel come into being used as an alternative source of energy as the diminishing of petroleum reserves. This fuel is typically stored in tanks that are commonly made from carbon steel, which is easily corroded by microorganisms. Recent studies have shown that bacteria aside from SRB may also be involved in corrosion. Therefore, this research was aimed to evaluate the effect of biodiesel concentration (15%, 20% and 30% v/v) mixed in diesel oil on the corrosion of carbon steel by S. marcescens that dominate biocorrosion on hydrocarbon products. In this study, the corrosion process was investigated by evaluation of biofilm morphology and composition, the rate of corrosion and the corrosion product of carbon steel which was exposed in the mixture of hydrocarbons and the presence of S. marcescens. It can be concluded that higher concentration of biodiesel in diesel oil leads to higher growth of bacteria in the biofilm and higher corrosion rate.

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Biosurfactant Production by Rhizospheric Bacteria Isolated from Biochar Amended Soil Using Different Extraction Solvents

Applied Environmental Research ◽

10.35762/aer.2019.41.3.6 ◽

2019 ◽

pp. 71-81

Author(s):

Seun O. Adebajo ◽

Aderonke K. Akintokun ◽

Abidemi E. Ojo ◽

Dami M. Egbagbe ◽

Pius O. Akintokun ◽

...

Keyword(s):

Methylene Blue ◽

Diesel Oil ◽

Alcaligenes Faecalis ◽

Biosurfactant Production ◽

Rhizospheric Bacteria ◽

Recovery Techniques ◽

Low Toxicity ◽

Extraction Solvents ◽

Surface Active Compounds ◽

Amended Soil

Microbial-derived surface-active compounds (biosurfactants) have attracted attention due to their low toxicity, cost-effectiveness, biodegradable nature and environment compatibility. Due to paucity of knowledge in the production of biosurfactant by microorganisms from other sources such as biochar-amended soil, the present study investigates the potential of rhizospheric bacteria isolated from biochar amended soil of okra plant in the production of biosurfactants using different recovery techniques. Rhizospheric bacteria were screened for biosurfactant production using Haemolytic, Oil spreading, Drop collapse, Methylene blue method, Bacterial adhesion to hydrocarbon and Emulsification activity. The biosurfactant was extracted using different extraction solvents (acid precipitation, ethyl acetate, acetone, dichloromethane and chloroform/methanol). Degradation of hydrocarbon (diesel) was determined spectrophotometrically. A total of twenty-three rhizospheric bacteria were isolated from the soil of Abelmoschus esculentus (okra plant). Nine isolates were positive for haemolysis with values between 1.1±0.2 mm by Enterobacter cloaca and 23.0±0.6 mm by Alcaligenes faecalis. Two isolates were positive for the drop collapse test. Only one isolate was positive for the methylene blue method. In the oil spreading test, ten isolates were positive and five isolates had the ability to adhere to hydrocarbons. Six isolates exhibited emulsification potential after 24 h, with the highest and lowest (65.9%) and (40.7%) recorded by Alcaligenes faecalis and Citrobacter sp, respectively. The biosurfactant produced by Alcaligenes faecalis using different recovery solvents showed that chloroform and methanol are the best extraction solvents and Alcaligenes faecalis was also able to degrade diesel oil over a period of 10 d. Conclusively, Alcaligenes faecalis recovered from soil amended sawdust biochar of okra plant is both a potent biosurfactant producer and an agent for remediating hydrocarbon-contaminated soil environments.

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