scholarly journals Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

2021 ◽  
Author(s):  
Svenja Nellinger ◽  
Ivana Mrsic ◽  
Silke Keller ◽  
Simon Heine ◽  
Alexander Southan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Cultured Cells ◽  
Structural Characteristics ◽  
Human Adipose Tissue ◽  
Minimal Invasive ◽  
Healthcare Applications ◽  
Decellularized Extracellular Matrix

Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively towards their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells (ASCs), towards their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans (sGAGs) compared to cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.

scholarly journals Decellularized Extracellular Matrix Derived from Porcine Adipose Tissue as a Xenogeneic Biomaterial for Tissue Engineering

2012 ◽  
Vol 18 (11) ◽  
pp. 866-876 ◽  
Author(s):  
Young Chan Choi ◽  
Ji Suk Choi ◽  
Beob Soo Kim ◽  
Jae Dong Kim ◽  
Hwa In Yoon ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Decellularized Extracellular Matrix

Cartilaginous Tissue Formation with an Elastic PLCL Scaffold and Human Adipose Tissue-Derived Stromal Cells

2007 ◽  
Vol 342-343 ◽  
pp. 385-388
Author(s):  
So Eun Lee ◽  
Young Mee Jung ◽  
Soo Hyun Kim ◽  
Sang Heon Kim ◽  
Jong Won Rhie ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Cartilage Tissue Engineering ◽  
Human Adipose Tissue ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Cartilaginous Tissue

In cartilage tissue engineering, as a cell source, adult stem cells are very attractive for clinical applications. Recent studies suggest that human adipose tissue-derived stromal cells (ASCs) have multilineage potential similar to bone marrow-derived stromal cells (BMSCs). ASCs are obtained from adipose tissue easily isolated by suction-assisted lipectomy in various body parts. Also, as one of major factors of cartilage tissue engineering, scaffolds have an important role in cartilage formation. Poly(L-lactide-co-ε-carprolactone) scaffolds have physiological activity, biodegradability, high cell affinity, and mechano-activity. The object of this study is cartilaginous tissue formation using highly elastic PLCL scaffolds and ASCs in vitro and in vivo. Poly(L-lactide-co-ε-carprolactone) copolymers were synthesized from lactide and ε-carprolactone in the presence of stannous octoate as catalyst. The scaffolds with 85% porosity and 300-500μm pore size were fabricated by gel-pressing method. ASCs were seeded on scaffolds and cultured for 21days in vitro. Cell/polymer constructs were characterized by reverse transcriptase-polymerase chain reaction for confirming differentiation to chondrocytes onto PLCL scaffolds. Also, for examining cartilaginous tissue formation in vivo, ASCs seeded scaffolds which were induced chondrogenesis for 2 weeks were implanted in nude mice subcutaneously for up to 8weeks. Histological studies showed that implants partially developed cartilaginous tissue within lacunae. And there was an accumulation of sulfated glycoaminoglycans. Immunohistochemical analysis revealed that implants were positively stained for specific extracellular matrix. These results indicate that ASCs and PLCL scaffols could be used to cartilage tissue engineering.

scholarly journals Attachment and Proliferation of Human-Adipose-Tissue-Derived Stem Cells on Bioactive Glass/PVA Hybrid Scaffolds

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Viviane Gomide ◽  
Alessandra Arcoverde Cavalcanti Zonari ◽  
Natalia Martins Breyner ◽  
Alfredo Miranda de Goes ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Bioactive Glass ◽  
Pore Structure ◽  
Bone Repair ◽  
Sol Gel ◽  
Human Adipose Tissue ◽  
Porous Scaffolds

Bioactive glass/polymer hybrids are promising materials for biomedical applications because they combine the bioactivity of bioceramics with the flexibility of polymers. These advantages may be used in porous scaffolds for tissue-engineering applications. In previous works, hybrid foams bioactive glass/polyvinyl alcohol (PVA) were prepared by the sol-gel process. The hybrid foam 50% PVA/50% bioactive glass was chosen in the composition range studied as the scaffold with better compromise in terms of pore structure and mechanical behavior. The aim of the present study was to evaluate the adhesion, viability, and growth behavior of human-adipose-tissue-derived stem cells on bioactive glass/PVA foams in vitro and their potential as scaffold for application in bone-tissue engineering. The pore structure of the hybrid samples used in the study was analyzed by microcomputed tomography, showing a modal pore diameter of 284 μm and modal interconnect diameter of 138 μm. We found that cells are capable of adhesion, proliferation, growth, and ECM production on the scaffolds tested. The results show that the hybrid bioactive glass/PVA is a promising material for bone repair, providing a good environment for the adhesion and proliferation of human-adipose-tissue-derived stem cells in vitro.

scholarly journals The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels

2021 ◽  
Vol 22 (22) ◽  
pp. 12560
Author(s):  
Su Hee Kim ◽  
Donghak Kim ◽  
Misun Cha ◽  
Soo Hyun Kim ◽  
Youngmee Jung
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Tissue Regeneration ◽  
Patient Specific ◽  
Adipose Derived Stem Cells ◽  
Tissue Formation ◽  
Pressing Method ◽  
Decellularized Extracellular Matrix

A dome-shaped elastic poly (l-lactide-co-caprolactone) (PLCL) scaffold with a channel and pore structure was fabricated by a combinative method of 3D printing technology and the gel pressing method (13 mm in diameter and 6.5 mm in thickness) for patient-specific regeneration. The PLCL scaffold was combined with adipose decellularized extracellular matrix (adECM) and heart decellularized extracellular matrix (hdECM) hydrogels and human adipose-derived stem cells (hADSCs) to promote adipogenesis and angiogenesis. These scaffolds had mechanical properties similar to those of native adipose tissue for improved tissue regeneration. The results of the in vitro real-time PCR showed that the dECM hydrogel mixture induces adipogenesis. In addition, the in vivo study at 12 weeks demonstrated that the tissue-engineered PLCL scaffolds containing the hydrogel mixture (hdECM/adECM (80:20)) and hADSCs promoted angiogenesis and adipose tissue formation, and suppressed apoptosis. Therefore, we expect that our constructs will be clinically applicable as material for the regeneration of patient-specific large-sized adipose tissue.

scholarly journals Adipose-Derived Stem Cells for Future Regenerative System Medicine

2012 ◽  
Vol 4 (2) ◽  
pp. 59
Author(s):  
Yani Lina ◽  
Andi Wijaya
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Adult Stem Cells ◽  
Stromal Vascular Fraction ◽  
Human Adipose Tissue ◽  
Lytic Enzymes ◽  
Stromal Stem Cells

BACKGROUND: The potential use of stem cell-based therapies for repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions for a number of disease. Despite the advances, the availability of stem cells remaining a challenge for both scientist and clinicians in pursuing regenerative medicine. CONTENT: Subcutaneous human adipose tissue is an abundant and accessible cell source for applications in tissue engineering and regenerative medicine. Routinely, the adipose issue is digested with collagenase or related lytic enzymes to release a heterogeneous population for stromal vascular fraction (SVF) cells. The SVF cells can be used directly or can be cultured in plastic ware for selection and expansion of an adherent population known as adipose-derived stromal/stem cells (ASCs). Their potential in the ability to differentiate into adipogenic, osteogenic, chondrogenic and other mesenchymal lineages, as well in their other clinically useful properties, includes stimulation of angiogenesis and suppression of inflammation.SUMMARY: Adipose tissue is now recognized as an accessible, abundant and reliable site for the isolation of adult stem cels suitable for the application of tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models.KEYWORDS: adipose tissue, adult stem cells, regenerative medicine, mesenchymal stem cells

IN VITRO STUDIES ON LIPOLYSIS IN HUMAN ADIPOSE TISSUE

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S156
Author(s):  
Suad Efendić ◽  
Peter Amer ◽  
Jan Östman
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
In Vitro Studies
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