scholarly journals Muscle Architecture In Patients With Sjogren's Syndrome

2020 ◽  
Author(s):  
Duygu Tecer ◽  
Dilek Büyükşireci ◽  
ZAFER GÜNENDİ ◽  
Jale Meray ◽  
Feride Gogus
Keyword(s):  
Muscle Strength ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Pennation Angle ◽  
Muscle Architecture ◽  
Anxiety And Depression ◽  
Healthy Controls ◽  
Isokinetic Muscle Strength ◽  
Ankle Muscle ◽  
Sjögren‘S Syndrome

Aim: To investigate the effect of primary (pSS) on skeletal muscle architecture and strength. Method: 19 pSS patients and 19 age, body mass index and sex matched healthy controls were included. Symptoms of subject’s were assessed with ESSPRI (The EULAR Sjogren’s Syndrome Patient Reported Index), anxiety and depression with the Hospital Anxiety and Depression Scale (HADS), fatigue with Multidimensional Assessment of Fatigue scale (MAF), functionality with HAQ. Ultrasonographic evaluations were performed with a multifrequency linear probe from quadriceps femoralis, gastrocnemius and soleus of the bilateral lower extremity. Muscle thickness, pennation angle and fascicle length were measured. Isokinetic knee muscle strength tests were performed at 60 and 180°/s. Isokinetic ankle muscle strenght tests were performed at 30 and 120°/s. Result: In pSS group, mean ESSPRI was 7.70±1.17. Scores of depression (10.05 ± 3.09 vs 4.47 ± 2.29 p<0.0001), anxiety (8.26 ± 4.28 vs 3.79 ± 2.42 p<0.0001), functionality (0.94 ± 0.78 vs 0.22 ± 0.26, p<0.0001), fatigue (37.69 ± 5.47 vs 17.69 ± 5.26, p<0.0001) were significantly higher in patients with pSS than healthy controls. In dominant leg, pennation angle of vastus medialis was significantly greater in healthy controls than in patients with pSS (p=0.049). The other ultrasonographic measurements were similar between the two groups. Peak torque/body weight of knee and ankle muscle were found to be similar. Conclusion: Although patients with pSS have some minor structural changes on ultrasonographic evaluation, isokinetic muscle strength were similar between groups. In patients with pSS, disease activity and fatigue level were negatively correlated with isokinetic muscle strength measurement.

scholarly journals POS0776 CORRELATION OF PERIPHERAL CD4+GRANZB+CTLS WITH DISEASE SEVERITY IN PATIENTS WITH PRIMARY SJÖGREN’S SYNDROME

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 641.3-642
Author(s):  
Q. Wang ◽  
L. Gu ◽  
M. Zhang
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Sjögren’S Syndrome ◽  
Mhc Class Ii ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Healthy Controls ◽  
Extraglandular Manifestations ◽  
Sjögren‘S Syndrome

Background:Sjögren’s syndrome (SS) is a chronic autoimmune disorder. The major histopathologic lesion of it is a focal lymphocytic infiltrate around ductal and acinar epithelial cells, which include a majority of CD4+T. Several studies have shown that the epithelial cells in SS present diverse phenomena, such as MHC class II overexpression. CD4+T cells with cytotoxic activity (CD4 CTL) have been detected in various immune responses. They are characterized by their ability to secrete perforin and granzyme B to kill the target cells in an MHC class II-restricted fashion.Objectives:So this study was to investigate the correlation of peripheral CD4+GranzB+CTLs with disease severity and organ involvement in patients with primary Sjögren’s syndrome.Methods:We recruited 116 pSS patients and 46 healthy controls using flow cytometry to examine proportion of CD4+GranzB+CTLs in their peripheral blood, and immunofluorescence to test the expression of CD4+GranzB+CTLs in labial gland. The correlations of CD4+GranzB+CTLs and the relevant clinical data were analyzed.Results:We analyzed the percentage of CD4+GranzB+cytotoxic T cells in peripheral blood mononuclear cells (PBMCs) by flow cytometry. Frequency of peripheral CD4+GranzB+CTLs were measured in 116 patients with pSS and 46 healthy controls matched for age and sex. The percentage of CD4+GranzB+CTLs were significantly up-regulated in pSS patients than healthy controls (7.1%±4.9% vs 3.1%±1.9%, p <0.0001) and positive correlation with ESSDAI in pSS patients(r = 0.6332, p<0.001). The percentage of CD4+GranzB+CTLs were markedly higher in pSS patients with extraglandular manifestations. Moreover, CD4+GranzB+CTLs were observed in the lymphocytic foci and periductal areas of the LSGs and were elevated with increased foci index (FI). After excluding the other risk factors associated with pSS, CD4+GranzB+CTLs were still related to ESSDIA and extraglandular manifestations independently(p<0.05). ROC curve analysis indicated that the area under the curve (AUC) of CD4+GranzB+CTLs was 0.796 to predict the activity of pSS, and 0.851 to presume extraglandular manifestations. The best diagnostic cut-off point was 4.865 for pSS patients.Conclusion:In this study, We provide new evidence indicating involvement of CD4+GranzB+CTLs over activation in the disease pathophysiology of pSS, which may serve as a new biomarker to evaluate the activity and severity of pSS.References:[1]Takeuchi A, Saito T. Front Immunol. (2017) 23:194.[2]Brown DM, et al. Front Immunol. (2016) 9:93.[3]Polihronis M, et al. Clin Exp Immunol. (1998) 114:485-90.[4]Xanthou G, et al. Clin Exp Immunol. (1999) 118:154-63.[5]Maehara T, et al. Ann Rheum Dis. (2017) 76:377-385.[6]Goules AV, et al. Clin Immunol. (2017) 182:30-40.[7]Hashimoto K, et al. Proc Natl Acad Sci U S A. (2019) 116:24242-24251.[8]Croia C, et al. Arthritis Rheumatol. (2014) 66:2545-57.[9]Schmidt D,et al. J Clin Invest. (1996) 97:2027–37.[10]Pandya JM, et al. Arthritis Rheum. (2010) 62:3457–66.[11]Moosig F, et al. Clin Exp Immunol. (1998) 114:113–8.[12]Peeters LM, et al. Front Immunol. (2017) 20:1160.Table 1.Multivariate analysis of CD4+GranzB+CTLs influenced by pSS-related factorsregression coefficientstandard errort-statisticsp value95%CICD8+GranzB+CTLs(%)0.1440.0334.3346.9E-50.077, 0.211ESSDAI0.2560.1222.0950.0410.011, 0.502extraglandular manifestations2.6121.2682.0590.0450.065, 5.158Figure 1.Receiver operating characteristic (ROC) curve of the frequency of CD4+GranzB+CTLs to predict ESSDAI and extraglandular manifestations responseDisclosure of Interests:None declared

Levels of plasmacytoid dendritic cells and type-2 myeloid dendritic cells are reduced in peripheral blood of patients with primary Sjögren's syndrome

2009 ◽  
Vol 69 (6) ◽  
pp. 1235-1238 ◽  
Author(s):  
Petra Vogelsang ◽  
Johan G Brun ◽  
Gunnvor Øijordsbakken ◽  
Kathrine Skarstein ◽  
Roland Jonsson ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Sjögren’S Syndrome ◽  
Peripheral Blood ◽  
Sjögren's Syndrome ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Healthy Controls ◽  
Myeloid Dendritic Cells ◽  
Antigen Presenting ◽  
Sjögren‘S Syndrome

ObjectiveSjögren's syndrome (SS) is a lymphoproliferative autoimmune disease, characterised by dryness of the mouth and eyes. Dendritic cells (DC) are potent antigen-presenting cells crucial for initiating and maintaining primary immune responses. This study quantified interferon-producing plasmacytoid DC (pDC) and two myeloid DC subsets (mDC1 and mDC2) in peripheral blood (PB) from primary SS (pSS) patients and healthy controls.MethodsBlood samples from 31 pSS patients and 28 gender and age-matched healthy controls were analysed by flow cytometry using the Miltenyi Blood DC enumeration kit. The presence of pDC in salivary glands (SG) from pSS patients was analysed by immunohistochemistry.ResultsPatients with pSS had significantly less pDC and mDC2 in PB compared with healthy controls. Moreover, pDC are present in SG from patients with pSS.ConclusionPatients with pSS have alterations among DC populations in PB, and pDC are present in the SG, suggesting a potential role of these cells in SS.

scholarly journals Chemosensory Dysfunction, Oral Disorders and Oral Health-Related Quality Of Life in Patients with Primary Sjögren's Syndrome: Comparative Cross-Sectional Study

2020 ◽  
Author(s):  
Mirjana Šijan Gobeljić ◽  
Vera Milić ◽  
Nada Pejnovic ◽  
Nemanja Damjanov
Keyword(s):  
Oral Health ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Primary Sjögren’S Syndrome ◽  
Healthy Controls ◽  
Primary Sjogren’S Syndrome ◽  
Age And Gender ◽  
Primary Sjögren's Syndrome ◽  
And Gender ◽  
Sjögren‘S Syndrome

Abstract Background: The aim of this study was to evaluate chemosensory function and oral disorders in patients with primary Sjögren's syndrome (pSS) and to compare these findings with those of age- and gender-matched healthy controls. Methods: Olfactory and gustatory function, dysgeusia, burning sensations in the tongue (BST), halitosis, and oral health-related quality of life (OHRQoL) using the short-form Oral Health Impact Profile (OHIP-14) were evaluated in 58 patients with primary Sjögren’s syndrome (pSS) and 55 age- and gender-matched healthy controls in this comparative cross-sectional study. Results: Patients with pSS had significantly lower self-reported visual analogue scale (VAS) smell score (8.6±2.2 vs. 9.6±0.7, p=0.016) and VAS taste score (9.5±0.7 vs. 8.5±2.1, p=0.014) than healthy controls. A greater proportion of patients with pSS had anosmia (3.8% vs. 0.0%) or hyposmia (36.5% vs. 13.2%) and ageusia for basic tastes: sweetness (34.0% vs. 7.5%), sourness (10.6 % vs. 0.0), saltiness (10.0% vs. 5.7%) or bitterness (19.1% vs. 1.9%) as evaluated using Sniffin Sticks test and taste stripts, respectively. A higher proportion of pSS patients complained of dysgeusia (52.6% vs. 9.4%, p<0.0001) and BST (45.6% vs. 0.0%, p<0.0001) while similar number of pSS patients and controls reported halitosis (31.6% vs. 28.3%, p=0.434). The mean OHIP-14 score was significantly higher in patients with pSS (6.8±7.0 vs. 2.3±8.5, p<0.001) indicating patients’ poorer OHRQoL compared to controls. Conclusions: The majority of patients with pSS had impaired chemosensory function and indicators of oral health in comparison to the age- and gender-matched healthy controls. Further studies of oral hygiene habits and dietary intake of these patients are needed to ensure better management of oral health problems in patients with pSS.

scholarly journals Chemosensory Dysfunction and Oral Disorders Affect Oral Health-Related Quality Of Life in Patients with Primary Sjögren's Syndrome

2020 ◽  
Author(s):  
Mirjana Šijan Gobeljić ◽  
Vera Milić ◽  
Nada Pejnovic ◽  
Nemanja Damjanov
Keyword(s):  
Oral Health ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Primary Sjögren’S Syndrome ◽  
Healthy Controls ◽  
Primary Sjogren’S Syndrome ◽  
Age And Gender ◽  
Primary Sjögren's Syndrome ◽  
And Gender ◽  
Sjögren‘S Syndrome

Abstract Background: The aim of this study was to evaluate chemosensory function and oral disorders in patients with primary Sjögren's syndrome (pSS) and to compare these findings with those of age- and gender-matched healthy controls.Methods: Olfactory and gustatory function, dysgeusia, burning sensations in the tongue (BST), halitosis, and oral health-related quality of life (OHRQoL) using the short-form Oral Health Impact Profile (OHIP-14) were evaluated in 58 patients with primary Sjögren’s syndrome (pSS) and 55 age- and gender-matched healthy controls.Results: Patients with pSS had significantly lower self-reported visual analogue scale (VAS) smell score (8.6±2.2 vs. 9.6±0.7, p=0.016) and VAS taste score (9.5±0.7 vs. 8.5±2.1, p=0.014) than healthy controls. A greater proportion of patients with pSS had anosmia (3.8% vs. 0.0%) or hyposmia (36.5% vs. 13.2%) and ageusia for basic tastes: sweetness (34.0% vs. 7.5%), sourness (10.6 % vs. 0.0), saltiness (10.0% vs. 5.7%) or bitterness (19.1% vs. 1.9%). A higher proportion of pSS patients complained of dysgeusia (52.6% vs. 9.4%, p<0.0001) and BST (45.6% vs. 0.0%, p<0.0001) while similar number of pSS patients and controls reported halitosis (31.6% vs. 28.3%, p=0.434). The mean OHIP-14 score was significantly higher in patients with pSS (6.8±7.0 vs. 2.3±8.5, p<0.001) indicating patients’ poorer OHRQoL compared to controls. Conclusions: The majority of patients with pSS had impaired chemosensory function and indicators of oral health in comparison to the age- and gender-matched healthy controls. Further studies of oral hygiene habits and dietary intake of these patients are needed to ensure better management of oral health problems in patients with pSS.

scholarly journals The Imbalance of Circulating Follicular T Helper Cell Subsets in Primary Sjögren’s Syndrome Associates With Serological Alterations and Abnormal B-Cell Distribution

2021 ◽  
Vol 12 ◽  
Author(s):  
Krisztina Szabó ◽  
Ilona Jámbor ◽  
Antónia Szántó ◽  
Ildikó Fanny Horváth ◽  
Tünde Tarr ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Primary Sjögren’S Syndrome ◽  
Healthy Controls ◽  
T Helper ◽  
Primary Sjogren’S Syndrome ◽  
Primary Sjögren's Syndrome ◽  
Sjögren‘S Syndrome

Since B-cell hyperactivity and pathologic antibody response are key features in the immunopathogenesis of primary Sjögren’s syndrome (pSS), the role of follicular T helper (TFH) cells as efficient helpers in the survival and differentiation of B cells has emerged. Our aim was to investigate whether a change in the balance of circulating (c)TFH subsets and follicular regulatory T (TFR) cells could affect the distribution of B cells in pSS. Peripheral blood of 38 pSS patients and 27 healthy controls was assessed for the frequencies of cTFH cell subsets, TFR cells, and certain B cell subpopulations by multicolor flow cytometry. Serological parameters, including anti-SSA, anti-SSB autoantibodies, immunoglobulin, and immune complex titers were determined as part of the routine diagnostic evaluation. Patients with pSS showed a significant increase in activated cTFH cell proportions, which was associated with serological results. Frequencies of cTFH subsets were unchanged in pSS patients compared to healthy controls. The percentages and number of cTFR cells exhibited a significant increase in autoantibody positive patients compared to patients with seronegative pSS. The proportions of transitional and naïve B cells were significantly increased, whereas subsets of memory B cells were significantly decreased and correlated with autoantibody production. Functional analysis revealed that the simultaneous blockade of cTFH and B cell interaction with anti-IL-21 and anti-CD40 antibodies decreased the production of IgM and IgG. Imbalance in TFH subsets and TFR cells indicates an ongoing over-activated humoral immune response, which contributes to the characteristic serological manifestations and the pathogenesis of pSS.

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