Microscopic investigation of trichomes of wild-growing Satureja montana L.

Considering the importance of S. montana L. as a medicinal plant, due to the essential oils produced in its glandular trichomes, and the lack of data about the leaf ultrastructure and micromorphology, we have carried out a comprehensive study of trichomes distributed in the vegetative and reproductive organs. Micromorphological and ultrastructural investigations of the glandular trichomes of Satureja montana L. and autofluorescence analyses of their secreted products were carried out using scanning electron microscopy (SEM), transmission electron microscopy (TEM), confocal laser scanning electron microscopy (CLSM) and light fluorescence microscope. The leaves, stem and calyx bear numerous nonglandular, unbranched trichomes and two types of glandular trichomes - peltate and capitate. Ultrastructural analyses showed the presence of mitochondria, plastids and proliferations of the granular endoplasmatic reticulum in secretory phases of the heads of glandular trichomes. Strong red autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM. Green-yellow autofluorescence of secreted material were noticed in peltate and capitate trichomes observed with a fluorescence microscope.

Download Full-text

Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)

Archives of Biological Sciences ◽

10.2298/abs0804667m ◽

2008 ◽

Vol 60 (4) ◽

pp. 667-672 ◽

Cited By ~ 7

Author(s):

Marija Marin ◽

Snezana Budimir ◽

Dusica Janosevic ◽

P.D. Marin ◽

Sonja Duletic-Lausevic ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Laser Scanning ◽

Glandular Trichomes ◽

Confocal Laser Scanning ◽

Confocal Laser ◽

Capitate Trichomes ◽

Histochemical Tests ◽

Scanning Electron

Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.

Download Full-text

The micromorphological, histochemical and confocal analysis of satureja subspicata Bartl. ex vis. glandular trichomes

Archives of Biological Sciences ◽

10.2298/abs1004143m ◽

2010 ◽

Vol 62 (4) ◽

pp. 1143-1149 ◽

Cited By ~ 9

Author(s):

Marija Marin ◽

N. Jasnic ◽

D. Lakusic ◽

Sonja Duletic-Lausevic ◽

Lia Ascensao

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Positive Reaction ◽

Laser Scanning ◽

Glandular Trichomes ◽

Confocal Laser Scanning ◽

Confocal Laser ◽

Histochemical Tests ◽

Scanning Electron

Micromorphology, histochemical and confocal analyses of the trichomes of Satureja subspicata (Bartl. ex Vis.) were carried out using light microscopy, confocal laser scanning electron microscopy (CLSM), and scanning electron microscopy. Non-glandular unbranched and two types of glandular trichomes - peltate and capitate - are described. The results of histochemical tests showed a positive reaction to phenolics, tannins, lipids, acid lipids, pectins and polysaccharides in both types of glandular trichomes. A strong red autofluorescence of the lipophilic and hydrophilic secreted material in glandular trichomes was observed with CLSM.

Download Full-text

Investigation of Spin Coating Cerium-Doped Hydroxyapatite Thin Films with Antifungal Properties

Coatings ◽

10.3390/coatings11040464 ◽

2021 ◽

Vol 11 (4) ◽

pp. 464

Author(s):

Simona Liliana Iconaru ◽

Mihai Valentin Predoi ◽

Patrick Chapon ◽

Sofia Gaiaschi ◽

Krzysztof Rokosz ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Photoelectron Spectroscopy ◽

Spin Coating ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Confocal Laser ◽

X Ray ◽

Antifungal Properties ◽

Scanning Electron

In this study, the cerium-doped hydroxyapatite (Ca10−xCex(PO4)6(OH)2 with xCe = 0.1, 10Ce-HAp) coatings obtained by the spin coating method were presented for the first time. The stability of the 10Ce-HAp suspension particles used in the preparation of coatings was evaluated by ultrasonic studies, transmission electron microscopy (TEM), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The surface morphology of the 10Ce-HAp coating was studied by SEM and atomic force microscopy (AFM) techniques. The obtained 10Ce-HAp coatings were uniform and without cracks or unevenness. Glow discharge optical emission spectroscopy (GDOES) and X-ray photoelectron spectroscopy (XPS) were used for the investigation of fine chemical depth profiling. The antifungal properties of the HAp and 10Ce-HAp suspensions and coatings were assessed using Candida albicans ATCC 10231 (C. albicans) fungal strain. The quantitative antifungal assays demonstrated that both 10Ce-HAp suspensions and coatings exhibited strong antifungal properties and that they successfully inhibited the development and adherence of C. albicans fungal cells for all the tested time intervals. The scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) visualization of the C. albicans fungal cells adherence to the 10Ce-HAp surface also demonstrated their strong inhibitory effects. In addition, the qualitative assays also suggested that the 10Ce-HAp coatings successfully stopped the biofilm formation.

Download Full-text