Antibody response of beef calves to experimental monovalent and multivalent inactivated bovine viral diarrhoea virus vaccines as measured by indirect elisa method

The objective of this study was to determine the immunogenic properties of two experimental inactivated (mono - and multivalent) vaccines containing BVDV type 1 reference strains (NADL, W1. - 162903, W2. - 172984, W3. - 173481, W4. - 179725) and one local field isolate derived from a calf suffering from mucosal disease (MD). Normal healthy beef calves (Simmental race) of mixed sex, 6 to 7 months of age, were divided into three experimental groups: ten calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated multivalent vaccine per animal (Group I); ten calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated monovalent vaccine per animal (Group II) and 9 unvaccinated calves (Control group C). Blood sera were obtained from immunized animals (standard procedure: on days 0, 14, 28, 42 and 56 post-immunization). The immune response to BVDV vaccine strains was assessed by the indirect ELISA method (Bovine Viral Diarrhoea Virus Antibody Test Kit BVDV HerdChek

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Establishing presence and status of infection with bovine diarrhoea virus in cattle herds in Central Serbia: Possibilities for control

Veterinarski glasnik ◽

10.2298/vetgl1002003k ◽

2010 ◽

Vol 64 (1-2) ◽

pp. 3-19

Author(s):

Vladimir Kurcubic ◽

Zoran Ilic ◽

Radojica Djokovic ◽

Slobodan Jevtic ◽

Tamas Petrovic

Keyword(s):

Blood Serum ◽

Bovine Viral Diarrhoea Virus ◽

Elisa Method ◽

Serum Samples ◽

Experimental Animals ◽

Test Kit ◽

Diarrhoea Virus ◽

Dairy Animals ◽

Cattle Herds ◽

Experimental Group

The objective of our investigations was to determine the presence and status of infection with the bovine viral diarrhoea virus (BVDV) and to identify persistently infected (PI) cattle in 4 herds (experimental groups): 2 with fattening heifers (10 and 13 animals) and 2 with dairy animals with offspring older than 4 months (23 and 16 animals). Two series of blood samples were taken in an interval of 4 weeks (even samples) from all the animals, from each experimental group. Separate blood serums from both groups of samples were examined using the indirect ELISA method (HerdChek*BVDV Ab test kit). The results obtained in our investigations indicate the presence of infection with the BVD virus in the experimental animals. Blood serum samples taken on the first day of investigation were seropositive in 7 experimental animals of the first group, in all (n=13) experimental animals of the second group, seropositive in one and suspect in 2 animals of the third experimental group, and seropositive in one and suspect in 3 experimental animals of the fourth group. Blood serum samples taken on the 28th day of the examinations were seropositive in one, thirteen, two, and three experimental animals of the first, second, third, and fourth experimental groups. Serum samples of animals (n=29) that were established as seronegative in both investigations (on the first and the 28th day) were examined for the possible presence of antigen to the BVD virus using the ELISA method (HerdChek*BVDV Ag/Serum Plus) with the objective of investigating the presence of PI animals. The presence of antigen to the BVD virus was not established in the examined cattle. On the grounds of the results of the investigations and data in literature, measures for controlling BVDV infections were recommended to animal owners. .

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Pathological lesions of lambs infected in utero with bovine viral diarrhoea virus type 1c (BVDV-1c)

Veterinary Record ◽

10.1002/vetr.6 ◽

2021 ◽

Vol 188 (3) ◽

pp. 187-196

Author(s):

Caitlin A. Evans ◽

Lucy Woolford ◽

Farhid Hemmatzadeh ◽

Michael P. Reichel ◽

Peter D. Cockcroft

Keyword(s):

Virus Type ◽

Bovine Viral Diarrhoea Virus ◽

In Utero ◽

Bovine Viral Diarrhoea ◽

Pathological Lesions ◽

Diarrhoea Virus

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Bovine viral diarrhoea virus 1b infection associated with congenital tremor and hypomyelination in Holstein calves

Veterinary Microbiology ◽

10.1016/j.vetmic.2021.109047 ◽

2021 ◽

pp. 109047

Author(s):

Laura Gallina ◽

Michel C. Koch ◽

Arcangelo Gentile ◽

Ida Treglia ◽

Cristiano Bombardi ◽

...

Keyword(s):

Bovine Viral Diarrhoea Virus ◽

Bovine Viral Diarrhoea ◽

Diarrhoea Virus ◽

Holstein Calves ◽

Congenital Tremor

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The use of a mass-action model to validate the output from a stochastic simulation model of bovine viral diarrhoea virus spread in a closed dairy herd

Preventive Veterinary Medicine ◽

10.1016/s0167-5877(96)01129-4 ◽

1997 ◽

Vol 31 (3-4) ◽

pp. 199-209 ◽

Cited By ~ 18

Author(s):

G. Innocent ◽

I. Morrison ◽

J. Brownlie ◽

G. Gettinby

Keyword(s):

Simulation Model ◽

Dairy Herd ◽

Bovine Viral Diarrhoea Virus ◽

Mass Action ◽

Virus Spread ◽

Bovine Viral Diarrhoea ◽

Mass Action Model ◽

Diarrhoea Virus ◽

Action Model ◽

Stochastic Simulation Model

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A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals

Acta Veterinaria Hungarica ◽

10.1556/avet.51.2003.2.11 ◽

2003 ◽

Vol 51 (2) ◽

pp. 229-236 ◽

Cited By ~ 8

Author(s):

Š. Vilček ◽

Jana Mojžišová ◽

Viera Bajová ◽

Š. Paulík ◽

L. Strojný ◽

...

Keyword(s):

Bovine Viral Diarrhoea Virus ◽

Computer Assisted ◽

Serological Survey ◽

Bovine Viral Diarrhoea ◽

Rt Pcr ◽

Serum Samples ◽

Genetic Typing ◽

Diarrhoea Virus ◽

Pcr Products ◽

Cattle Farms

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

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Anti-Bovine Viral Diarrhoea Virus Antibodies in Adult Zambian Patients Infected with the Human Immunodeficiency Virus

International Journal of STD & AIDS ◽

10.1177/095646249300400510 ◽

1993 ◽

Vol 4 (5) ◽

pp. 300-302 ◽

Cited By ~ 5

Author(s):

M Giangaspero ◽

G Vacirca ◽

D Morgan ◽

K S Baboo ◽

N P Luo ◽

...

Keyword(s):

Synergistic Effects ◽

Bovine Viral Diarrhoea Virus ◽

Hiv Infections ◽

Chronic Diarrhoea ◽

Bovine Viral Diarrhoea ◽

Diarrhoea Virus ◽

Immunodeficiency Virus ◽

Mechanism Of Interaction ◽

Asymptomatic Individuals ◽

Hiv Seropositive

Bovine viral diarrhoea (BVD) virus is a cosmopolitan pestivirus of animals which is associated with diarrhoea, immunosuppression and synergy with other pathogens. This study was conducted to establish the prevalence of anti-BVD virus antibodies in healthy Zambian adults and those with asymptomatic and symptomatic HIV disease. Sera from 1159 adults were tested for anti-BVD virus antibodies using the indirect immunofluorescence test and the confirmatory Western blot. Of the 1159 sera examined, 180 (15.5%) showed significantly elevated titres of anti-BVD antibodies. These included 70 out of 477 (14.7%) HIV-negative healthy adults; 73 out of 480 (15.2%) of HIV-positive asymptomatic individuals; 23 out of 129 (17.8%) HIV-seropositive patients with associated illnesses excluding diarrhoea; and 14 out of 73 (19.2%) of HIV-seropositive patients with chronic diarrhoea. HIV-seropositive patients with chronic diarrhoea or associated illnesses appear to have significantly increased seroprevalence of anti-BVD virus antibodies ( P = >0.01). The mechanism of interaction between BVD virus and HIV infections and the synergistic effects with other opportunistic pathogens in humans requires definition.

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