scholarly journals Cultivation conditions preferable for yeast cells to be immobilized into poly(vinyl alcohol) and used in bottled sparkling wine production

2006 ◽  
Vol 12 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Elena Efremenko ◽  
Nikolay Stepanov ◽  
Nikolay Martinenko ◽  
Irina Gracheva
Keyword(s):  
Vinyl Alcohol ◽  
Immobilized Cells ◽  
Yeast Cells ◽  
Poly Vinyl Alcohol ◽  
Sparkling Wine ◽  
Wine Production ◽  
Biochemical Status ◽  
Viability Level ◽  
Yeast Immobilization ◽  
High Level

An effective approach to the improvement of the biochemical status of yeast cells before their immobilization was applied to develop a biocatalyst with a high level of cell metabolic activity and viability that could be used for sparkling wine production. According to the approach, the wine-containing medium (pH 3.0) routinely used for the accumulation of champagne yeast biomass under aerobic conditions was replaced by a semi-synthetic one (pH 5.6), usually applied for aerobic yeast growth. The variation of temperature and pH conditions of cell growth showed a modification of the fatty acid pool of yeasts and its importance for the further immobilization of cells into a poly(vinyl alcohol) cryogel. Measurement of the specific intracellular ATP concentration by the bioluminescent method revealed the growth phase favorable for yeast immobilization. The main characteristics of the sparkling wine obtained after four-week fermentation with application of both the free and immobilized cells were similar but, according to the detected energetic status, the viability level of the immobilized cells was considerably higher compared to the free yeast. The CO2 pressure accumulated in the bottles with immobilized cells (up to 500 kPa) after fermentation also appeared notably higher than in the bottles with free yeast.

Application of a New Organic Yeast Immobilization Method for Sparkling Wine Production

2013 ◽  
Vol 64 (3) ◽  
pp. 386-394 ◽  
Author(s):  
Anna Puig-Pujol ◽  
Eva Bertran ◽  
Teresa García-Martínez ◽  
Fina Capdevila ◽  
Santiago Mínguez ◽  
...  
Keyword(s):  
Sparkling Wine ◽  
Wine Production ◽  
Yeast Immobilization ◽  
Immobilization Method

Graphene-reinforced poly(vinyl alcohol) electrospun fibers as building blocks for high performance nanocomposites

RSC Advances ◽  
2015 ◽  
Vol 5 (103) ◽  
pp. 85009-85018 ◽  
Author(s):  
Sajjad Ghobadi ◽  
Sina Sadighikia ◽  
Melih Papila ◽  
Fevzi Çakmak Cebeci ◽  
Selmiye Alkan Gürsel
Keyword(s):  
Polymer Matrix ◽  
Vinyl Alcohol ◽  
High Performance ◽  
Building Blocks ◽  
Poly Vinyl Alcohol ◽  
Electrospun Fibers ◽  
Matrix Solution ◽  
High Level

Graphene-containing fibrous structures with a high level of affinity towards a polymer matrix solution have been proved to be promising for high performance macroscopic nanocomposite reinforcement purposes.

scholarly journals Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

Catalysts ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 326 ◽  
Author(s):  
Olga Senko ◽  
Marina Gladchenko ◽  
Olga Maslova ◽  
Elena Efremenko
Keyword(s):  
Clostridium Acetobutylicum ◽  
Immobilized Cells ◽  
Poly Vinyl Alcohol ◽  
Anaerobic Sludge ◽  
Lignocellulosic Waste ◽  
Long Term Storage ◽  
Anaerobic Consortium ◽  
High Level ◽  
Term Storage

The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional” introduction of bacterial Clostridium acetobutylicum, Pseudomonas sp., Enterococcus faecalis cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge.

scholarly journals “Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

Catalysts ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 33 ◽  
Author(s):  
◽  
Keyword(s):  
Bacterial Cellulose ◽  
Vinyl Alcohol ◽  
Immobilized Cells ◽  
Jerusalem Artichoke ◽  
Poly Vinyl Alcohol ◽  
Cellulose Synthesis ◽  
Cellulose Production ◽  
Bacterial Cellulose Production ◽  
Renewable Biomass ◽  
Repeated Use

A new biocatalyst in the form of Komagataeibacter xylinum B-12429 cells immobilized in poly(vinyl alcohol) cryogel for production of bacterial cellulose was demonstrated. Normally, the increased bacteria concentration causes an enlarged bacterial cellulose synthesis while cells push the polysaccharide out to pack themselves into this polymer and go into a stasis. Immobilization of cells into the poly(vinyl alcohol) cryogel allowed “deceiving” them: bacteria producing cellulose pushed it out, which further passed through the pores of cryogel matrix and was accumulated in the medium while not covering the cells; hence, the latter were deprived of a possible transition to inactivity and worked on the synthesis of bacterial cellulose even more actively. The repeated use of immobilized cells retaining 100% of their metabolic activity for at least 10 working cycles (60 days) was performed. The immobilized cells produce bacterial cellulose with crystallinity and porosity similar to polysaccharide of free cells, but having improved stiffness and tensile strength. Various media containing sugars and glycerol, based on hydrolysates of renewable biomass sources (aspen, Jerusalem artichoke, rice straw, microalgae) were successfully applied for bacterial cellulose production by immobilized cells, and the level of polysaccharide accumulation was 1.3–1.8-times greater than suspended cells could produce.

Yeast cells in double layer calcium alginate–chitosan microcapsules for sparkling wine production

2019 ◽  
Vol 300 ◽  
pp. 125174 ◽  
Author(s):  
Ilaria Benucci ◽  
Martina Cerreti ◽  
Diamante Maresca ◽  
Gianluigi Mauriello ◽  
Marco Esti
Keyword(s):  
Double Layer ◽  
Calcium Alginate ◽  
Yeast Cells ◽  
Sparkling Wine ◽  
Wine Production

scholarly journals Sparkling wine production by immobilised yeast fermentation

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 171-179 ◽  
Author(s):  
Miličević Borislav ◽  
Babić Jurislav ◽  
Ačkar Đurđica ◽  
Miličević Radoslav ◽  
Jozinović Antun ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Alginate Beads ◽  
Aroma Compounds ◽  
Yeast Cells ◽  
Yeast Fermentation ◽  
Sparkling Wine ◽  
Alcohol Content ◽  
Wine Production ◽  
Grape Varieties ◽  
Immobilised Yeast

The prospects of sparkling wine production by the ‘Champenoise’ method using alginate-immobilised yeast cells were examined. Grape varieties dominant in quantity were selected within the group of recommended and permitted varieties of Kutjevo vineyards, located in the eastern part of continental Croatia. Research revealed that there are no influential variations in the principal physicochemical and sensory characteristics between sparkling wines obtained through immobilised yeast and traditional sparkling method. The analysis of aroma compounds showed minor differences between samples. Observed oenological parameters assessed in the final products did not show any relevant oenological differences, with the exception of alcohol content, which was slightly higher in sparkling wines made with yeast cells immobilised with calcium alginate beads. According to this research, the sensory properties of the produced sparkling wines, compared to sparkling wine produced with free yeast, did not show any significant differences. On the full-scale obtained results indicate that some of the selected varieties can be sorted as suitable for the production of sparkling wine using immobilised yeast cells.

scholarly journals “Nature-like” Cryoimmobilization of Phototrophic Microorganisms: New Opportunities for Their Long-Term Storage and Sustainable Use

2022 ◽  
Vol 14 (2) ◽  
pp. 661
Author(s):  
Olga Senko ◽  
Nikolay Stepanov ◽  
Olga Maslova ◽  
Elena Efremenko
Keyword(s):  
Immobilized Cells ◽  
Sustainable Use ◽  
Biomass Accumulation ◽  
Poly Vinyl Alcohol ◽  
High Concentration ◽  
Long Term Storage ◽  
Phototrophic Microorganisms ◽  
High Level ◽  
Term Storage

It was found that immobilization of cells in poly(vinyl alcohol) (PVA) cryogel can be successfully applied for concurrent cryoimmobilization, cryoconservation and long-term storage of the cells of various phototrophic microorganisms (green and red microalgae, diatoms and cyanobacteria). For the first time, it was shown for 12 different immobilized microalgal cells that they can be stored frozen for at least 18 months while retaining a high level of viability (90%), and can further be used as an inoculum upon defrosting for cell-free biomass accumulation. Application of cryoimmobilized Chlorella vulgaris cells as inocula allowed the loading of a high concentration of the microalgal cells into the media for free biomass accumulation, thus increasing the rate of the process. It was shown that as minimum of 5 cycles of reuse of the same immobilized cells as inocula for cell accumulation could be realized when various real wastewater samples were applied as media for simultaneous microalgae cultivation and water purification.

scholarly journals Raman Microspectroscopy of the Yeast Vacuoles

2012 ◽  
Vol 27 ◽  
pp. 503-507 ◽  
Author(s):  
Lucie Bednárová ◽  
Jan Palacký ◽  
Václava Bauerová ◽  
Olga Hrušková-Heidingsfeldová ◽  
Iva Pichová ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Immobilized Cells ◽  
Raman Microspectroscopy ◽  
Stress Factors ◽  
Yeast Cells ◽  
Multivariate Methods ◽  
Label Free ◽  
Spatially Resolved ◽  
Confocal Raman Microspectroscopy ◽  
Yeast Immobilization

In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was investigated and critically assessed. Simple, economical, and practical protocols of the yeast immobilization suitable for less laborious, high-throughput, and spatially resolved Raman measurements were tested for their possible impacts on physiological states and viability of the cells. We have demonstrated that, acquiring Raman spectra from statistically sound sets of immobilized cells and employing advanced multivariate methods for spectral analysis, the chemical composition of the yeast vacuoles can be reliably studied. The most easily and accurately quantifiable seems to be the concentration of polyphosphates which can be unambiguously identified due to unmistakable Raman features. Our approach can be useful for routine, label-free, and noninvasive monitoring of the chemical composition of the vacuoles of living yeasts exposed to various stress factors, the information important in biomedical research of pathogens.

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