scholarly journals Correlation between retention and C-18 silica gel surface coverage in reversed-phase liquid chromatography

2000 ◽  
Vol 65 (11) ◽  
pp. 811-818
Author(s):  
Marijana Acanski ◽  
Slobodan Petrovic ◽  
Vjera Pejanovic ◽  
Julijana Petrovic
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carbon Content ◽  
Silica Gel ◽  
High Performance ◽  
Surface Coverage ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Retention Behaviour ◽  
Phase Liquid

The effect of C-18 silica gel surface coverage on the retention behaviour of some estrone derivatives in reversed phase high performance liquid chromatography has been studied. Two commercial columns with different C-18 coverage, Spherisorb ODS-1(8 % carbon content) and Li Chrosorb RP-18(22% carbon content), using methanol-water as the eluent, were used.

Determination of erythrocytic polyamines by reversed-phase liquid chromatography

1991 ◽  
Vol 37 (12) ◽  
pp. 2117-2120 ◽  
Author(s):  
Lucile Gerbaut
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Water Gradient ◽  
Phase Liquid ◽  
Dansyl Derivatives

Abstract A simple and rapid semiautomated procedure for determining polyamines in erythrocytes by high-performance liquid chromatography is described. Putrescine, spermidine, and spermine are converted to fluorescent dansyl derivatives, extracted with cyclohexane, and separated in <10 min on a reversed-phase C18 ODS column, with an acetonitrile-water gradient as the mobile phase. The method showed a coefficient of variation of 2.73% for spermidine and 3.27% for spermine. The respective reference values, evaluated in 10 healthy patients, were 7.88 (SD 2.09) and 5.42 (SD 1.55) μmol/L of packed erythrocytes. Only negligible amounts of putrescine were found.

Free and total putrescine in cerebrospinal fluid quantified by reversed-phase liquid chromatography.

1984 ◽  
Vol 30 (7) ◽  
pp. 1243-1245 ◽  
Author(s):  
R L Heideman ◽  
K B Fickling ◽  
L J Walker
Keyword(s):  
Cerebrospinal Fluid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Radial Compression ◽  
Fluid Sample ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Sensitive Method

Abstract This is a simple, rapid, sensitive method for routine quantification of the polyamine putrescine (1,4-diaminobutane) in cerebrospinal fluid. Sample preparation involves protein precipitation, acid hydrolysis for 18 h (if total putrescine is to be measured), pre-column derivatization with o-phthalaldehyde, and extraction into acetonitrile. The derivative is separated and quantified by "high-performance" liquid chromatography on a reversed-phase C18 radial-compression column. A single chromatographic run takes less than 18 min. Putrescine concentrations as low as 50 nmol/L in cerebrospinal fluid can be detected.

Streaming current detector for reversed-phase liquid chromatography

1981 ◽  
Vol 59 (10) ◽  
pp. 1531-1537 ◽  
Author(s):  
Shigeru Terabe ◽  
Kiyoshi Yamamoto ◽  
Teiichi Ando
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Streaming Current ◽  
Glass Capillaries ◽  
Phase Liquid

Fundamental characteristics of the electrokinetic detector for high performance liquid chromatography, whose operating principle is based on the measurement of the streaming current between both ends of a bundle of glass capillaries, were studied under reversed-phase chromatographic conditions. The detector is specifically sensitive to ionizable compounds like carboxylic acids and amines, but is also universally responsive to nonionic compounds. The detection limit for the former compounds was about 10−8 g and for the latter about 10−4 g. The lowest amount actually measured was 5.8 × 10−9 g. The universal detectability makes this type of detector unsuitable for gradient elution. The observed streaming current was in the range of 10−7 to 10−8 A and was highly dependent on the flow rate of the mobile phase. Characteristics of the detectors equipped with some packed beds prepared from porous materials instead of glass capillaries were also investigated.

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