scholarly journals In vitro evaluation of coronal microleakage associated with two root obturation techniques

2007 ◽  
Vol 54 (3) ◽  
pp. 184-190 ◽  
Author(s):  
Slavoljub Zivkovic ◽  
Borislava David-Burlica ◽  
Jelena Stanisic ◽  
Sonja Stojcic ◽  
Milica Medojevic-Jovanovic ◽  
...  

Aim: The purpose of this study was to determine the influence of the smear layer on the coronal microleakage in root canals obturated with laterally condensed cold gutta-percha or Thermafil and two root canal sealers. Materials and methods: Sixty extracted single-rooted human teeth with straight root canals were allocated to two experimental groups. The root canals were instrumented using the STEP-BACK technique, K reamers and K files and irrigated with 2.5 % NaOCl. The teeth in the first group were treated with 17% EDTA and 2.5 % NaOCl to remove the smear layer. The teeth in both groups were obturated either with laterally condensed cold guttapercha (28 teeth) or Thermafil gutta-percha with plastic cores (28 teeth) and RSA or AH-PLUS sealers. The specimens were evaluated for coronal microleakage using silver nitrate solution. The linear penetration of the dye was assessed using a binocular magnifying glass (x25), with an inserted micrometer scale. Results: The results showed low coronal microleakage in the group where the smear layer was removed in both the lateral compaction technique (RSA - 1.71mm and AHPLUS - 1.61mm) and the Thermafil groups - RSA (1.65mm) and AH-PLUS (1.66mm). In the group with the intact smear layer, greater microleakage was noticed in both groups, lateral compaction technique -RSA (3.06 mm) and AH-PLUS (2.83mm) and Thermafil technique -RSA (2.99mm) and AH-PLUS ( 2.83mm). The difference was statistically significant between the groups with and without the smear layer for both RSA (p< 0.001) and AH-PLUS sealers ( p<0.001). Conclusion: The present results suggest that the removal of the smear layer significantly improves the tightness of the coronal seal and reduces the coronal microleakage.

2008 ◽  
Vol 55 (2) ◽  
pp. 99-106
Author(s):  
Aleksandra Djeri ◽  
S. Sukara ◽  
R. Arbutina ◽  
Z. Trnic ◽  
N. Gajic

Introduction: Coronal microleakage is one of the most important factors for the success and longevity of endodontically treated teeth. Aim: The aim of this study was to evaluate in vitro the coronal microleakage in carious and intact teeth obturated with lateral compaction of gutta-percha using the dye penetration method. Materials and Methods: Forty extracted human teeth were allocated to two groups: Group I consisted of 20 intact teeth (10 single-rooted and 10 multi-rooted) and Group II comprised 20 carious teeth (10 single-rooted and 10 multi-rooted). Root canal preparation was performed using the Step-back technique and copious irrigation with 2.5% NaOCl. All root canals were obturated using lateral compaction and AH Plus endodontic sealer. The teeth were coronally filled with either CAVIT only or the combination of GIC and CAVIT, coated with 2 layers of nail varnish and immersed in 50% AgNO3. Afterwards, the teeth were sectioned mesio-distally using a diamond disc and linear dye penetration was evaluated using a stereo loupe with a micrometer scale at x6 magnification. Results: The results showed dye penetration in 70% of teeth filled with CAVIT and in 10% of teeth filled with GIC+CAVIT. The difference was statistically significant (p<0.05). Conclusion: Based on the obtained results, it may be concluded that the least coronal microleakage was observed in intact teeth temporarily filled with the GIC+CAVIT combination.


2007 ◽  
Vol 54 (4) ◽  
pp. 224-230
Author(s):  
Dejan Markovic ◽  
Vesna Zivojinovic ◽  
Slavoljub Zivkovic

Objective: An in vitro comparison of coronal microleakage of three root canal sealers was performed using the dye leakage method. Methodology: Ninety-eight single-rooted human teeth were instrumented and randomly allocated to two groups. In the first group, root canals were treated with EDTA to remove the smear layer. The roots in both groups were obturated using laterally condensed gutta-percha and Sealapex, AH-26 or Ketac-Endo as the sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then rinsed, sectioned perpendicular to the long axis and evaluated for coronal dye penetration. Results: In general, Ketac-Endo root canal sealer showed greater dye penetration than Sealapex and AH-26. A statistically significant difference in the coronal penetration of the dye was found in the group with intact smear layer. Conclusion: Highly significant differences between AH-26 and Ketac-Endo (p < 0.001) and Sealapex and Ketac Endo (p < 0.001) were found.


2008 ◽  
Vol 55 (4) ◽  
pp. 221-228
Author(s):  
Violeta Pavlovic ◽  
Slavoljub Zivkovic

Aim: To evaluate the effect of irrigation techniques, i.e. different types of irrigation needles, on the quality of cleaning of root canal walls using scanning electron microscopy (SEM). Material and Method: The study was conducted on 16 extracted, single-rooted, human teeth. The samples were allocated to two experimental groups depending on the type of the irrigation needle. Conventional needles were used for the irrigation of Group I and laterally perforated ones for Group II. All root canals were instrumented using K files and the Step-back technique. During instrumentation, all samples were irrigated with 2.5 % sodium hypochlorite solution followed by final irrigation with 17 % EDTA for 1 min. After that, all samples were irrigated with 10 ml of distilled water. The roots were, then, sectioned longitudinally and middle one third of each root canal was analyzed using SEM. Quantitative analysis was based on criteria by H?lsmann et al. Results: The obtained results showed that the more efficient removal of debris and the smear layer was accomplished in the group of samples irrigated using laterally perforated needles than conventional needles. The difference between the investigated irrigation techniques were statistically significant (p<0.01). Conclusion: The use of laterally perforated needles for irrigation allows more efficient cleaning of root canal walls, i.e. the removal of debris and the smear layer.


2012 ◽  
Vol 13 (6) ◽  
pp. 830-833
Author(s):  
Romel Joseph

ABSTRACT Aim The aim of this in vitro study was to evaluate the apical seal obtained with four root canal sealers AH 26, Sealapex, Endoflas FS and AH Plus, with lateral condensation. Materials and methods Sixty root canals were prepared using the step-back technique. The specimens were divided into four experimental groups of 12 teeth and two control groups of 12 teeth. The experimental groups were obturated by laterally condensed gutta-percha with one of the tested sealers and control groups were obturated without any sealer. Methylene blue dye penetration with centrifuging method was used to evaluate the apical sealing ability. The quantitative apical leakage of each specimen was measured after 2 weeks. Results The results showed no significant differences between all groups except between AH Plus and Endoflas FS (<0.05). AH Plus showed significantly less leakage than Endoflas FS. Conclusion AH Plus showed the least leakage compared to AH 26, Sealapex and Endoflas FS. How to cite this article Joseph R, Singh S. Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study. J Contemp Dent Pract 2012;13(6):830-833.


2006 ◽  
Vol 14 (3) ◽  
pp. 219-223 ◽  
Author(s):  
Nicole de Mello Rahde ◽  
José Antonio Poli Figueiredo ◽  
Elias Pandonor Motcy Oliveira

PURPOSE: The aim of this study was to evaluate, in vitro, the quality of calcium hydroxide [Ca(OH)2] paste filling (Ultracal, Ultradent) associated or not with Ca(OH)2-containing gutta-percha points (Calcium Hydroxide Plus PointsTM, Roeko) in curved root canals. MATERIAL AND METHODS: One hundred and twenty roots of extracted human teeth, randomly divided into three curvature ranges (mild - 0 to 14º; moderate - 15º to 29º; severe - >30º) were used. After chemomechanical preparation, the roots were assigned to 4 groups (n=30), according to the technique of intracanal dressing placement: group 1 - Ca(OH)2 paste was applied with a lentulo spiral; group 2 - Ca(OH)2 paste was applied with a lentulo spiral and a Ca(OH)2 point was inserted into the canal; group 3 - Ca(OH)2 paste was applied with a NavitipTM tip (supplied with Ultracal system); group 4 - Ca(OH)2 paste was applied with a NavitipTM tip and a Ca(OH)2 point was inserted into the canal. The roots were cleared and the quality of apical third filling was assessed by a calibrated experienced examiner. The specimens were examined under stereomicroscopy and scored 1 to 4 (i.e., from inadequate to complete root canal filling). The results were analyzed statistically by ANOVA and Duncan's post hoc test at 5% significance level. RESULTS: There were no statistically significant differences (p>0.05) among the curvature degrees in groups 1, 3 and 4. Severely curved roots in group 2 presented bordering significance (p=0.05). The groups that associated the use of Ca(OH)2 paste and points (2 and 4) showed better apical filling than the other groups, but this difference was statistically significant (p<0.001) only for roots with severe curvature. CONCLUSION: According to the results of this study, the curvature degree did not influence the quality of filling. The techniques that used Ca(OH)2-containing gutta-percha points yielded better filling of the apical third in roots with severe curvature.


2006 ◽  
Vol 20 (3) ◽  
pp. 231-234 ◽  
Author(s):  
Eliana Barbosa de Souza ◽  
Crystiane Venditti Gomes de Amorim ◽  
José Luiz Lage Marques

Apical sealing is essential for the success of paraendodontic surgery, so any procedure that may favor an adequate sealing of the apical remainder should be performed. The purpose of this study was to evaluate the influence of diode laser irradiation on the apical sealing of root-end cavities with MTA retrofillings. Root canals in twenty extracted human teeth were shaped with K-files and filled with gutta-percha. The apexes were cut off and root-end preparations were performed. The roots were divided randomly in 2 groups. Group 1 (ten specimens) was retrofilled with MTA. Group 2 was irradiated with diode laser, with 1 W for 20 seconds, on the apical surface and root end cavity before retrofilling with MTA. The specimens had their external surfaces impermeabilized with cyanoacrylate, except for the apical surface, and were then immersed in 1% rhodamine B dye for 72 h and placed in plaster stone. After that, the specimens were submitted to longitudinal abrasion until half of the root remained. The linear dye leakage was observed in these mid-roots between the root canal wall and retrofilling. The linear dye leakage was measured with Image Lab software, and the results were statistically analyzed with Student's t test. There were no statistically significant differences between the two groups (p > 0.05). The diode laser irradiation did not improve the apical sealing of MTA retrofillings under the conditions of this in vitro study.


2005 ◽  
Vol 13 (2) ◽  
pp. 176-179 ◽  
Author(s):  
Juliana Machado Barroso ◽  
Laise Daniela Carrasco ◽  
Alexandre Capelli ◽  
Danilo Mathias Zanello Guerisoli ◽  
Paulo César Saquy ◽  
...  

The aim of this study was to investigate, in vitro, the percentage of filling of simulated lateral canals in teeth obturated with TP medium and standardized gutta-percha points. Twenty human mandibular canines were prepared with LA Axxess (SybronEndo) and K³ Endo rotary system (SybronEndo) up to a #50 file, according to the Free Tip Preparation Technique. During instrumentation, the root canals were alternately irrigated with 1% sodium hypochlorite and 17% EDTA. Six artificial lateral canals were prepared at the apical third of each tooth. Then, the teeth were assigned to two groups (n=10): Group 1 - filled with TP medium master gutta-percha points (Dentsply, Mailleffer); Group 2 - filled with standardized master gutta-percha points (Dentsply, Mailleffer). Root canal filling was complemented with AH Plus sealer (Dentsply, Mailleffer) and accessory gutta-percha points (Dentsply, Mailleffer), according to the classic technique. The teeth were radiographed and the images obtained were digitized. Linear measurements of the percentage of filling of the artificial lateral canals in each group were accomplished on the Image Tool 2.02 software. Statistical analysis of the data using Mann-Whitney U non-parametric test evidenced significant difference (p<0.01) between the experimental groups. The group obturated with TP medium points yielded higher percentage of filling of the lateral canals. It may be concluded that the use of master gutta-percha points with larger taper resulted in better filling of the simulated lateral canals, as compared to the use of standardized master gutta-percha points.


2006 ◽  
Vol 14 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Márcia Carneiro Valera ◽  
Carlos Henrique Ribeiro Camargo ◽  
Alessandra Sverberi Carvalho ◽  
Eduardo Ramalho Pereira Gama

The objective of this study was to evaluate the apical leakage of retrograde cavities filled with Portland Cement (Concrebrás S/A-MG-Brazil), ProRoot MTA TM (Dentsply International, Johnson City, TN, USA) and Sealapex (Kerr Corporation, Orange, California, USA) with addition of zinc oxide (Odahcam Herpo Produtos Dentários Ltda, Rio de Janeiro, RJ, Brazil). Forty-two extracted single-rooted human teeth were decoronated and used for this study. The root canals were instrumented at 1.0mm short of the apical foramen using the step-back technique to an apical ISO size 60. The roots were obturated with gutta-percha points and sealer Sealapex (Kerr Corporation-USA) and then 3mm of each root apex was sectioned at a 90° angle. Ultrasonic retrograde preparation was performed with a diamond tip to 3mm depth and the roots were randomly divided into 3 groups according to the filling material: G1-Portland, G2-ProRoot MTA, G3- Sealapex zinc oxide-added cement. The root surfaces were covered with nail varnish up to 2mm from the apical foramen, immersed in simulated tissue fluid for 30 days, and then immersed in 0.2% Rhodamine B solution for 24 hours for evaluation of marginal leakage. The results showed mean leakage of 0.75, 0.35 and 0.35 for groups 1, 2 and 3, respectively; however, Kruskal-Wallis test revealed that there was no statistically significant difference among the results (p>0.05).


2004 ◽  
Vol 5 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Oya Bala ◽  
L. Sibel Karadag ◽  
Emin Türköz ◽  
Tansev Mihçioğlu

Abstract In this study, the aim was to assess the in vitro apical microleakage of a resin-based sealer used with two different adhesives. Thirty nine freshly extracted maxillary incisors were used. The teeth were decoronated at the cemento-enamel junction with a water-cooled fissure bur. Chemo-mechanical debridement of the root canals was accomplished with the step-back technique. The smear layer was removed by 19% ethylenediamine tetra acetic acid (EDTA). The roots were then divided into three experimental groups of thirteen teeth in each. Specimens in group 1 were filled with gutta-percha, AH Plus sealer, and water-based adhesive system (Syntac Single Component). Group 2 specimens were filled with gutta-percha, AH Plus sealer, and acetone-based dentin adhesive (Prime & Bond NT). Specimens of group 3 were filled with only gutta-percha and AH Plus sealer (no adhesive was applied). The teeth were immersed into 2% methylene blue solution. Apical sealing qualities were assessed by measuring the linear dye penetration with a stereomicroscope. Dentin tubule penetration was observed under scanning electron microscopy (SEM). Results showed no statistically significant difference between the materials used, however, the leakage in group 2 was less than group 1 and 3. Citation Karadag LS, Bala O, Türköz E, Mihçioğlu T. The Effects of Water and Acetone-based Dentin Adhesives on Apical Microleakage. J Contemp Dent Pract 2004 May;(5)2:093-101.


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