scholarly journals Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1

2010 ◽  
Vol 64 (3-4) ◽  
pp. 159-167
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Ruzica Asanin ◽  
Aleksandra Knezevic ◽  
Jelena Asanin ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Lines ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Clinical Symptoms ◽  
Parallel Implementation ◽  
Chain Reaction ◽  
Three Samples ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus

Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.

scholarly journals Development of a Probe Based Real Time PCR Assay for Detection of Bovine Herpes Virus-1 in Semen and Other Clinical Samples

2012 ◽  
Vol 24 (1) ◽  
pp. 16-26
Author(s):  
Basavegowdanadoddi Marinaik Chandranaik ◽  
Doddamane Rathnamma ◽  
S. S. Patil ◽  
Ramesh C. Kovi ◽  
Jyotsana Dhawan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Bovine Herpes Virus 1 ◽  
Herpès Virus

Evaluation of different sampling methods and results of real-time PCR for detection of feline herpes virus-1,Chlamydophila felisandMycoplasma felisin cats

2008 ◽  
Vol 11 (6) ◽  
pp. 375-380 ◽  
Author(s):  
Titti Sjödahl-Essén ◽  
Anna Tidholm ◽  
Peter Thorén ◽  
Annie Persson-Wadman ◽  
Göran Bölske ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Sampling Methods ◽  
Herpès Virus

scholarly journals Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis

2008 ◽  
Vol 92 (7) ◽  
pp. 928-932 ◽  
Author(s):  
S Sugita ◽  
N Shimizu ◽  
K Watanabe ◽  
M Mizukami ◽  
T Morio ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Virus Genome ◽  
Human Herpes ◽  
Human Herpes Virus ◽  
Ocular Fluids ◽  
Herpès Virus

scholarly journals DETERMINASI MOLEKULER KOI HERPES VIRUS (KHV) YANG DIISOLASI DARI IKAN KOI (Cyprinus carpio koi)

2012 ◽  
Vol 8 (2) ◽  
pp. 64
Author(s):  
Jetti Treslah Saselah ◽  
Reiny A Tumbol ◽  
Henky Manoppo
Keyword(s):  
Polymerase Chain Reaction ◽  
Cyprinus Carpio ◽  
Herpes Virus ◽  
Clinical Symptoms ◽  
The Body ◽  
Chain Reaction ◽  
Fish Samples ◽  
Polymerase Chain ◽  
Herpès Virus ◽  
Cyprinus Carpio Koi

Penelitian ini bertujuan untuk mendeterminasi keberadaan Koi Herpes Virus (KHV) pada ikan koi (Cyprinus carpio koi). Sampel ikan diambil dari Kabupaten Kepulauan Sangihe. Penelitian di lakukan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil pemeriksaan PCR terhadap sampel ikan koi mengindikasikan bahwa sampel ikan koi telah terinfeksi oleh virus KHV, yang ditandai dengan munculnya pita DNA pada hasil visualisasi elektroforesis agarosa. Hasil pemeriksaan pada morfologi ternyata sampel ikan yang digunakan menunjukkan gejala-gejala klinis terserang KHV seperti mata pucat, insang berwarna pucat serta produksi lendir yang berlebihan. Beberapa sampel lainnya walaupun secara morfologi belum menunjukan gejala-gejala klinis tetapi melalui pemeriksaan PCR telah terindikasikan terinfeksi KHV. Kata kunci: KHV, PCR, ikan koi   The purpose of this study was to determine the presence of Koi Herpes Virus (KHV) in Koi (Cyprinus carpio koi). Fish samples were taken from the Sangihe Archipelago Regency. Laboratory work conducted at the Laboratory of Biology Faculty of Mathematics and Natural Sciences University of Sam Ratulangi using Polymerase Chain Reaction (PCR) method. The samples indicated that the koi fish samples were infected with KHV. It is shown by the appearance of DNA bands on agarose electrophore­sis visualization. The morphological examination of the body indicated clinical symptoms of KHV infection, such as pale eyes, pale gills and excessive mucus production. Several other samples, although without clinical symptoms, have shown to be infected as indicated by PCR test. Keywords: KHV, PCR, Koi.

Quantitative detection of human herpes virus 1 & 2 using the real time PCR STAT-NAT HSV1 & 2 assays

2019 ◽  
Vol 493 ◽  
pp. S565-S566
Author(s):  
M. Gramegna ◽  
G. Ferri ◽  
L. Bavagnoli ◽  
M.L. Incandela ◽  
D. Rigamonti ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Herpes Virus ◽  
Quantitative Detection ◽  
Human Herpes ◽  
The Real ◽  
Human Herpes Virus ◽  
Herpès Virus
Sign in / Sign up

Export Citation Format

Share Document