scholarly journals Lyophilization as a method for pathogens long term preservation

2007 ◽  
pp. 203-210 ◽  
Author(s):  
Mirjana Milosevic ◽  
Sladjana Medic-Pap ◽  
Maja Ignjatov ◽  
Dragana Petrovic
Keyword(s):  
Pseudomonas Syringae ◽  
Plant Material ◽  
Freeze Drying ◽  
Plant Pathogens ◽  
Development Stage ◽  
Vegetable Crops ◽  
Nutritive Medium ◽  
Long Time ◽  
Long Term Preservation

Lyophilization (freeze-drying) is one of the most suitable methods used for a long term preservation of pathogens. The aim of this paper was the application of lyophilization for storage of three significant plant pathogens: Fusarium graminearum, Helminthosporium gramineum, and Pseudomonas syringae pv. gylicinea, respectively. The plant material was collected continuously (during a four year period 2002-2006), depending on a plant development stage, from different localities in Vojvodina. Pathogens were isolated from diseased parts with characteristic symptoms, and placed on nutritive media specific for a certain pathogen, using standard phytopathological methods. Lyophilization was carried out in marked and coded ampoules by freezing and drying of pathogen suspension and nutritive medium. Revitalization of lyophilized isolates was done after four days. High percentage of revitalization was characteristic for all studied isolates, and it ranged from 85-92%, confirming that lyophilized pathogens would be capable of keeping viability for a long time in the collection. Besides above mentioned pathogens, there were 200 isolates in the collection, originating mostly from field and vegetable crops. Each isolate that was put into the Collection, was followed by all the necessary data such as: name of the pathogen, number of isolates, locality, host plant year of isolation, name of the researcher and other relevant data.

scholarly journals Long-Term Preservation of Mouse Spermatozoa after Freeze-Drying and Freezing Without Cryoprotection1

2003 ◽  
Vol 69 (6) ◽  
pp. 2100-2108 ◽  
Author(s):  
Monika A. Ward ◽  
Takehito Kaneko ◽  
Hirokazu Kusakabe ◽  
John D. Biggers ◽  
David G. Whittingham ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Long Term Preservation

Results of long-term preservation of Mycobacteria by means of freeze-drying

Cryobiology ◽  
1976 ◽  
Vol 13 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Milan Šlosárek ◽  
Jiří Šourek ◽  
Zdeňka Miková
Keyword(s):  
Freeze Drying ◽  
Long Term Preservation

Preservation of living fungi pathogenic for man and animals

1974 ◽  
Vol 20 (12) ◽  
pp. 1665-1673 ◽  
Author(s):  
W. Butterfield ◽  
S. C. Jong ◽  
M. T. Alexander
Keyword(s):  
Liquid Nitrogen ◽  
Freeze Drying ◽  
Culture Collection ◽  
American Type Culture Collection ◽  
Subsequent Storage ◽  
Long Term Preservation

The paper describes the materials and procedures presently used at the American Type Culture Collection (ATCC) for the long-term preservation of living fungi pathogenic for man and animals by freeze-drying and by freezing and subsequent storage in liquid nitrogen. Longevity storage data for the strains available for distribution is presented.

scholarly journals Long-term preservation of Pastuerella piscicida by freezing or freeze-drying methods.

1989 ◽  
Vol 55 (6) ◽  
pp. 979-984
Author(s):  
Shinichi Hashimoto ◽  
Aiichiro Muraoka ◽  
Riichi Kusuda
Keyword(s):  
Freeze Drying ◽  
Drying Methods ◽  
Long Term Preservation

scholarly journals Successful Long-Term Preservation of Rat Sperm by Freeze-Drying

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e35043 ◽  
Author(s):  
Takehito Kaneko ◽  
Tadao Serikawa
Keyword(s):  
Freeze Drying ◽  
Long Term Preservation

scholarly journals Orchid cryopreservation

2014 ◽  
Vol 38 (3) ◽  
pp. 213-229 ◽  
Author(s):  
Wagner Vendrame ◽  
Ricardo Tadeu de Faria ◽  
Mauren Sorace ◽  
Sandra Aparecida Sahyun
Keyword(s):  
Plant Material ◽  
Storage Time ◽  
Natural Habitat ◽  
Orchid Species ◽  
Commercial Use ◽  
The Family ◽  
Relevant Field ◽  
Long Term Preservation ◽  
Intermediate Seeds

Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low maintenance costs that provide greater storage time and that enable good phytosanitary conditions for the plant material for commercial use. Cryopreservation involves the conservation of biological materials at ultra-low temperatures, generally in liquid nitrogen at -196 ºC or in its vapor phase at -150 ºC. This is the only technique currently available for the long-term preservation of the germplasm of plant species that are vegetatively propagated or that have unviable, recalcitrant or intermediate seeds. The objective of this bibliographic review is to report on the importance, methods and application of cryopreservation for orchids. According to the studies reviewed, this is an incipient, developing and relevant field that generates a lot of discussion and requires further research relative to the type of treatment to use for cryopreservation and the methodology to be applied according to the species. The types of methods that are used for cryopreservation and the large variation in the responses of orchids to the cryopreservation methods observed in this study emphasize the need for the development of more appropriate protocols for the preservation of orchids.

A simple MWCNTs@paper biosensor for CA19-9 detection and its long-term preservation by vacuum freeze drying

2020 ◽  
Vol 144 ◽  
pp. 995-1003
Author(s):  
Weiqiu Jin ◽  
Ruotong Zhang ◽  
Changzi Dong ◽  
Tianshu Jiang ◽  
Yilong Tian ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Long Term Preservation

scholarly journals Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

2020 ◽  
Vol 6 ◽  
pp. 1-7
Author(s):  
Wagner Vendrame ◽  
Roberto Takane ◽  
Lamartine Cardoso ◽  
Leonardo Alvarez ◽  
Ricardo Tadeu
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Liquid Nitrogen ◽  
Plant Material ◽  
Germination Percentage ◽  
Endangered Plant ◽  
Human Influence ◽  
Seed Moisture ◽  
Long Term Preservation

The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to human influence. Two of them, Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k., are endemic of the Brazilian Caatinga. Cryopreservation can provide a means for long-term preservation of endangered plant material. The aim of this study was to evaluate the efficiency of cryoprotectants for the cryopreservation of seeds from these two ­species. The treatments consisted of immersing seeds in different cryoprotectant solutions and vitrification solutions before storage in liquid nitrogen (NL) at -196 °C, as follows: T1- Control: no cryoprotectants; T2- PVS2 (10 min), T3-  PVS2 + phloroglucinol 1% (10 min), T4- PVS2 (10 min) + Supercool 1%; T5- PVS2 + phloroglucinol 1% (10 min) + Supercool 1%. Seed germination was evaluated after cryopreservation. The experimental design was completely randomized with five treatments and five replicates per treatment. Seed moisture at the beginning of the experiment was 6.2% for M. zehntneri and 7.8% for C. gounellei. There were no statistically significant differences for the seed germination percentage among the different treatments. Therefore, we concluded that seed of these two cacti species can be cryopreserved directly without the need for cryoprotectant solutions.

Alternative strategies for the long-term preservation of spermatozoa

1997 ◽  
Vol 9 (3) ◽  
pp. 309 ◽  
Author(s):  
W. V. Holt
Keyword(s):  
Freeze Drying ◽  
Reproductive Technologies ◽  
Considerable Improvement ◽  
Future Research ◽  
Germplasm Preservation ◽  
Recent Success ◽  
Testicular Cells ◽  
Freeze Dried ◽  
Long Term Preservation

A number of perceived future requirements for stored germplasm in agriculture, aquaculture, biotechnology and conservation are discussed in the present review. In the light of these broad demands, it is apparent that current approaches to gamete and embryo storage need considerable improvement, and that novel approaches to the technologies of germplasm preservation should be pursued if possible. The present article is presented in response to a request for novel future research ideas in this area. Early literature on desiccation, and later research into natural mechanisms of survival during desiccation, is considered in relation to the development of freeze-drying and vitrification methods. Developments in reproductive technologies themselves may mean that freeze-dried spermatozoa could realistically be used for direct intracytoplasmic microinjection of oocytes. Other radical methods that may achieve germplasm preservation are harvesting testicular cells in culture, cryopreserving immature or seasonally regressed testicular material, or a combination of both. Evidence from non-mammalian testicular culture systems, and recent success with the transmeiotic development of mouse spermatidsin vitro, suggest that these approaches may eventually become feasible.

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