scholarly journals Orchid cryopreservation

2014 ◽  
Vol 38 (3) ◽  
pp. 213-229 ◽  
Author(s):  
Wagner Vendrame ◽  
Ricardo Tadeu de Faria ◽  
Mauren Sorace ◽  
Sandra Aparecida Sahyun
Keyword(s):  
Plant Material ◽  
Storage Time ◽  
Natural Habitat ◽  
Orchid Species ◽  
Commercial Use ◽  
The Family ◽  
Relevant Field ◽  
Long Term Preservation ◽  
Intermediate Seeds

Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low maintenance costs that provide greater storage time and that enable good phytosanitary conditions for the plant material for commercial use. Cryopreservation involves the conservation of biological materials at ultra-low temperatures, generally in liquid nitrogen at -196 ºC or in its vapor phase at -150 ºC. This is the only technique currently available for the long-term preservation of the germplasm of plant species that are vegetatively propagated or that have unviable, recalcitrant or intermediate seeds. The objective of this bibliographic review is to report on the importance, methods and application of cryopreservation for orchids. According to the studies reviewed, this is an incipient, developing and relevant field that generates a lot of discussion and requires further research relative to the type of treatment to use for cryopreservation and the methodology to be applied according to the species. The types of methods that are used for cryopreservation and the large variation in the responses of orchids to the cryopreservation methods observed in this study emphasize the need for the development of more appropriate protocols for the preservation of orchids.

Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3-O-Gallate

2009 ◽  
Vol 18 (5-6) ◽  
pp. 513-520 ◽  
Author(s):  
Hakhee Kim ◽  
Takeshi Kawazoe ◽  
Kazuaki Matsumura ◽  
Shigehiko Suzuki ◽  
Suong-Hyu Hyon
Keyword(s):  
Storage Time ◽  
Radical Scavenging ◽  
Glucose Consumption ◽  
Skin Grafts ◽  
Dermal Matrix ◽  
Skin Sample ◽  
Biopsy Specimens ◽  
Previously Treated ◽  
Long Term Preservation

Skin grafts can be preserved by cryopreservation and refrigerated storage at 4°C. Epigallocatechin-3- O-gallate (EGCG) enhances the viability of stored skin grafts and also extends the storage time up to 7 weeks at 4°C. EGCG, the major polyphenolic constituent present in green tea, has potent antioxidant, antimicrobial, antiproliferative, and free radical scavenging effects. This study examined the effects of EGCG on skin cryopreservation. Skin sample biopsy specimens from GFP rats were previously treated with/without EGCG then moved to −196°C. Skin samples were transplanted to nude mice after 2, 8, and 24 weeks of preservation. Glucose consumption was measured after thawing to assess the metabolic activity. Two weeks later the transplanted skin grafts were excised and histologically analyzed. Histological examinations revealed the degeneration of the epidermal and dermal layers in all groups. In the EGCG groups, the grafts showed higher integrity in the epidermal layer and dermal matrix. The present findings suggest the future clinical usefulness of EGCG for skin preservation; however, the mechanism by which EGCG promotes skin preservation still remains unclear.

scholarly journals Lyophilization as a method for pathogens long term preservation

2007 ◽  
pp. 203-210 ◽  
Author(s):  
Mirjana Milosevic ◽  
Sladjana Medic-Pap ◽  
Maja Ignjatov ◽  
Dragana Petrovic
Keyword(s):  
Pseudomonas Syringae ◽  
Plant Material ◽  
Freeze Drying ◽  
Plant Pathogens ◽  
Development Stage ◽  
Vegetable Crops ◽  
Nutritive Medium ◽  
Long Time ◽  
Long Term Preservation

Lyophilization (freeze-drying) is one of the most suitable methods used for a long term preservation of pathogens. The aim of this paper was the application of lyophilization for storage of three significant plant pathogens: Fusarium graminearum, Helminthosporium gramineum, and Pseudomonas syringae pv. gylicinea, respectively. The plant material was collected continuously (during a four year period 2002-2006), depending on a plant development stage, from different localities in Vojvodina. Pathogens were isolated from diseased parts with characteristic symptoms, and placed on nutritive media specific for a certain pathogen, using standard phytopathological methods. Lyophilization was carried out in marked and coded ampoules by freezing and drying of pathogen suspension and nutritive medium. Revitalization of lyophilized isolates was done after four days. High percentage of revitalization was characteristic for all studied isolates, and it ranged from 85-92%, confirming that lyophilized pathogens would be capable of keeping viability for a long time in the collection. Besides above mentioned pathogens, there were 200 isolates in the collection, originating mostly from field and vegetable crops. Each isolate that was put into the Collection, was followed by all the necessary data such as: name of the pathogen, number of isolates, locality, host plant year of isolation, name of the researcher and other relevant data.

scholarly journals Cryoprotectant treatment tests on three morphologically diverse marine dinoflagellates and the cryopreservation of Breviolum sp. (Symbiodiniaceae)

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Joseph Kanyi Kihika ◽  
Susanna A. Wood ◽  
Lesley Rhodes ◽  
Kirsty F. Smith ◽  
Lucy Thompson ◽  
...  
Keyword(s):  
Genetic Drift ◽  
Rapid Freezing ◽  
Diverse Group ◽  
Limited Success ◽  
The Family ◽  
Agricultural Applications ◽  
Marine Dinoflagellates ◽  
Long Term Preservation ◽  
Cryopreservation Protocol

AbstractDinoflagellates are among the most diverse group of microalgae. Many dinoflagellate species have been isolated and cultured, and these are used for scientific, industrial, pharmaceutical, and agricultural applications. Maintaining cultures is time-consuming, expensive, and there is a risk of contamination or genetic drift. Cryopreservation offers an efficient means for their long-term preservation. Cryopreservation of larger dinoflagellate species is challenging and to date there has been only limited success. In this study, we explored the effect of cryoprotectant agents (CPAs) and freezing methods on three species: Vulcanodinium rugosum, Alexandrium pacificum and Breviolum sp. A total of 12 CPAs were assessed at concentrations between 5 and 15%, as well as in combination with dimethyl sulfoxide (DMSO) and other non-penetrating CPAs. Two freezing techniques were employed: rapid freezing and controlled-rate freezing. Breviolum sp. was successfully cryopreserved using 15% DMSO. Despite exploring different CPAs and optimizing the freezing techniques, we were unable to successfully cryopreserve V. rugosum and A. pacificum. For Breviolum sp. there was higher cell viability (45.4 ± 2.2%) when using the controlled-rate freezing compared to the rapid freezing technique (10.0 ± 2.8%). This optimized cryopreservation protocol will be of benefit for the cryopreservation of other species from the family Symbiodiniaceae.

scholarly journals Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

2020 ◽  
Vol 6 ◽  
pp. 1-7
Author(s):  
Wagner Vendrame ◽  
Roberto Takane ◽  
Lamartine Cardoso ◽  
Leonardo Alvarez ◽  
Ricardo Tadeu
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Liquid Nitrogen ◽  
Plant Material ◽  
Germination Percentage ◽  
Endangered Plant ◽  
Human Influence ◽  
Seed Moisture ◽  
Long Term Preservation

The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to human influence. Two of them, Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k., are endemic of the Brazilian Caatinga. Cryopreservation can provide a means for long-term preservation of endangered plant material. The aim of this study was to evaluate the efficiency of cryoprotectants for the cryopreservation of seeds from these two ­species. The treatments consisted of immersing seeds in different cryoprotectant solutions and vitrification solutions before storage in liquid nitrogen (NL) at -196 °C, as follows: T1- Control: no cryoprotectants; T2- PVS2 (10 min), T3-  PVS2 + phloroglucinol 1% (10 min), T4- PVS2 (10 min) + Supercool 1%; T5- PVS2 + phloroglucinol 1% (10 min) + Supercool 1%. Seed germination was evaluated after cryopreservation. The experimental design was completely randomized with five treatments and five replicates per treatment. Seed moisture at the beginning of the experiment was 6.2% for M. zehntneri and 7.8% for C. gounellei. There were no statistically significant differences for the seed germination percentage among the different treatments. Therefore, we concluded that seed of these two cacti species can be cryopreserved directly without the need for cryoprotectant solutions.

Preservation of guinea pig hearts by hypothermic gas perfusion

1989 ◽  
Vol 67 (7) ◽  
pp. 692-696
Author(s):  
Leslie E. Bailey
Keyword(s):  
Guinea Pig ◽  
Storage Time ◽  
Contractile Activity ◽  
Myocardial Damage ◽  
Perfusate Flow ◽  
Transplant Center ◽  
Tissue Calcium ◽  
Oxygen Requirements ◽  
Long Term Preservation

The lack of a satisfactory method for long-term preservation of hearts during transport limits the source of human hearts for transplant to the geographic vicinity of the transplant center. Experimentally, reduction of myocardial oxygen requirements with hypothermia and cardioplegia prolong storage time to 48 h, but always with some evidence of myocardial damage. In this study, the combination of hypothermia with a procedure known to increase oxygen tension in cardiac muscle, gas perfusion, preserved contractile activity in guinea pig hearts for 24 h and did not cause edema. Cardioplegia or gas perfusion at temperatures below 10 °C or above 20 °C resulted in failure of hearts to contract upon rewarming. Contracture, dehydration, elevation of tissue calcium, reduced perfusate flow, and elevated creatine kinase levels occurred if liquid reperfusion was begun at 15 °C but not 25 °C. The results suggest that under the appropriate conditions, hypothermic gas perfusion is a potentially useful means of extending storage time of hearts for transplant.Key words: myocardial preservation, heart storage, hypothermia, gas perfusion, contractility.

Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽  
2020 ◽  
pp. 65-69
Author(s):  
T.E. Lipatkina ◽  
◽  
Е.V. Karlova ◽  
A.V. Zolotarev ◽  
◽  
...  
Keyword(s):  
Macular Edema ◽  
Primary Open Angle Glaucoma ◽  
Antihypertensive Drugs ◽  
Open Angle Glaucoma ◽  
Vascular Wall ◽  
Open Angle ◽  
Retinal Edema ◽  
Long Term Preservation ◽  
Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

Etude de l'aménagement d'une forêt classée au Mali avec la participation des populations riveraines - Données du problème et éléments de méthodologie | A Study of the Management of a State-Owned Forest in Mali with the Participation of the Population Living nearby - Issue under Investigation and Elements of Methodology

2000 ◽  
Vol 151 (3) ◽  
pp. 80-83
Author(s):  
Pascal Schneider ◽  
Jean-Pierre Sorg
Keyword(s):  
Natural Resources ◽  
Research Methodology ◽  
Large Scale ◽  
Environmental Context ◽  
Forest Resources ◽  
The State ◽  
Commercial Use ◽  
Economic Context ◽  
The People

In and around the state-owned forest of Farako in the region of Sikasso, Mali, a large-scale study focused on finding a compromise allowing the existential and legitimate needs of the population to be met and at the same time conserving the forest resources in the long term. The first step in research was to sketch out the rural socio-economic context and determine the needs for natural resources for autoconsumption and commercial use as well as the demand for non-material forest services. Simultaneously, the environmental context of the forest and the resources available were evaluated by means of inventories with regard to quality and quantity. According to an in-depth comparison between demand and potential, there is a differentiated view of the suitability of the forest to meet the needs of the people living nearby. Propositions for a multipurpose management of the forest were drawn up. This contribution deals with some basic elements of research methodology as well as with results of the study.

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