From Golden Horn to Golden Gate: The Flight of the Russian Siberian Flotilla

1985 ◽  
Vol 64 (4) ◽  
pp. 290-294
Author(s):  
Michael Shimkin ◽  
Mary Shimkin
Keyword(s):  
2018 ◽  
Author(s):  
V  Leunov ◽  
Y  Mikheev

Расширение ассортимента потребляемых овощных культур – одна из основных задач в селекционно-семеноводческой работе с редечными культурами на Дальнем Востоке. В статье приводятся данные о селекции редечных культур (лобы, дайкона, редьки и др.) на Приморской овощной опытной станции. В результате проведения многолетних исследований и реализации ряда научных программ был создан новый исходный материал для селекции. В настоящем материале также подведены итоги актуальных направлений в селекции редечных культур в условиях муссонного климата. Селекционно-семеноводческие исследования в условиях климата Дальнего Востока с использованием индивидуального, а также индивидуально-семейственного методов отбора, разработка и внедрение технологических приемов первичного и сортового семеноводства обеспечили создание перспективного селекционного материала. Выделены ценные генисточники для создания новых сортообразцов китайского, японского и европейского подвидов редьки с высокими иммунологическими, продуктивными и товарными качествами, повышенными биохимическими показателями, высокой устойчивостью к переувлажнению почвы и возбудителями болезней. Созданы и включены в Госреестр РФ высокопродуктивные сорта китайского подвида редьки (лоба) Малиновый шар и Золотой рог, европейского подвида зимней редьки Ночная красавица, устойчивые к переувлажнению почвы и цветушности, бактериальным заболеваниям. В результате более чем двадцатилетних исследований изучены морфологические и биологические особенности формирования семенных растений редьки, использование эффективных элементов отбора в сочетании с агротехническими приемами возделывания. Усовершенствованы технологические методы первичного и товарного семеноводства редьки, выделены благоприятные агроклиматические зоны выращивания семенных растений, способствующие получению высококачественных семян при сохранении ими высоких биологических и хозяйственных признаков. Для условий муссонного климата Дальнего Востока внедрена в производство система первичного и сортового семеноводства редьки с использованием пересадочной и беспересадочной технологии выращивания. Результаты работы открывают дорогу к созданию новых высокоурожайных российских сортов и гибридов редечных культур.Expansion of the range of consumption of vegetable crops is one of the main tasks in the breeding and seed work with regions cultures in the Far East. The article presents data on the breeding radishes cultures (loba, daikon, radish, etc.) on the Maritime vegetable experiment station. As a result of years of research and implementation of a number of research programs has created a new source material for breeding. In this paper also summarize the current trends in the selection of radishes cultures in conditions of monsoonal climate. Breeding and seed research in the climate conditions of the Far East with the use of individual and individual and family methods of selection, development and implementation of technological methods of primary and varietal seed production ensured the development of advanced breeding material. Allocated valuable genitocracy to create new varieties of Chinese, Japanese and European subspecies of radishes cultures with high immunological, productive and commercial qualities, elevated biochemical parameters, high resistance to waterlogging and soil pathogens. Created and included in the state register of the Russian Federation high-yielding varieties of Chinese subspecies of radish (loba) a Crimson ball and the Golden horn, the European subspecies winter radish Night beauty, resistant to waterlogging of the soil and bolting and bacterial diseases. As a result of more than twenty years studied the morphological and biological peculiarities of formation of seed plants of radishes, the use of effective elements of selection in combination with agrotechnical methods of cultivation. Improved technological methods of primary and commercial seed radishes allocated to favorable agro-climatic zones of cultivation of seed plants, which would produce high-quality seeds while preserving their high biological and economic characteristics. For the conditions of monsoon climate of the Far East implemented in the production system of primary and varietal seed radishes using transit and non-stop growing technologies. The results of this work open the way to the creation of new high-yielding Russian varieties and hybrids of radishes cultures.


2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Marcos Valenzuela-Ortega ◽  
Christopher French

Abstract Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly of complex multipart constructs from reusable basic DNA parts in a rapid, reliable and automation-friendly way. Many such toolkits are available, with varying degrees of compatibility, most of which are aimed at specific host organisms. Here, we present a vector design which allows simple vector modification by using modular cloning to assemble and add new functions in secondary sites flanking the main insertion site (used for conventional modular cloning). Assembly in all sites is compatible with the PhytoBricks standard, and vectors are compatible with the Standard European Vector Architecture (SEVA) as well as BioBricks. We demonstrate that this facilitates the construction of vectors with tailored functions and simplifies the workflow for generating libraries of constructs with common elements. We have made available a collection of vectors with 10 different microbial replication origins, varying in copy number and host range, and allowing chromosomal integration, as well as a selection of commonly used basic parts. This design expands the range of hosts which can be easily modified by modular cloning and acts as a toolkit which can be used to facilitate the generation of new toolkits with specific functions required for targeting further hosts.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Simon J. Moore ◽  
Yonek B. Hleba ◽  
Sarah Bischoff ◽  
David Bell ◽  
Karen M. Polizzi ◽  
...  

Abstract Background  A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to study genes/pathways flexibly using Escherichia coli heterologous expression. In this current work, we sought to use EcoFlex to optimise a synthetic raspberry ketone biosynthetic pathway. Raspberry ketone is a high-value (~ £20,000 kg−1) fine chemical farmed from raspberry (Rubeus rubrum) fruit. Results  By applying a synthetic biology led design-build-test-learn cycle approach, we refactor the raspberry ketone pathway from a low level of productivity (0.2 mg/L), to achieve a 65-fold (12.9 mg/L) improvement in production. We perform this optimisation at the prototype level (using microtiter plate cultures) with E. coli DH10β, as a routine cloning host. The use of E. coli DH10β facilitates the Golden Gate cloning process for the screening of combinatorial libraries. In addition, we also newly establish a novel colour-based phenotypic screen to identify productive clones quickly from solid/liquid culture. Conclusions  Our findings provide a stable raspberry ketone pathway that relies upon a natural feedstock (L-tyrosine) and uses only constitutive promoters to control gene expression. In conclusion we demonstrate the capability of EcoFlex for fine-tuning a model fine chemical pathway and provide a range of newly characterised promoter tools gene expression in E. coli.


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