Infectivity and Attenuation of Leishmania donovani Promastigotes: Association of Galactosyl Transferase with Loss of Parasite Virulence

1999 ◽  
Vol 85 (1) ◽  
pp. 54 ◽  
Author(s):  
Tripti De ◽  
Syamal Roy
Keyword(s):  
Leishmania Donovani ◽  
Galactosyl Transferase

Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells

1990 ◽  
Vol 48 (3) ◽  
pp. 944-945
Author(s):  
Ichiro Yamamoto ◽  
Toshiaki Tachibana ◽  
Hiroko Maruyama ◽  
Noriyuki Komatsu ◽  
Hiroyuki Kuramoto ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Cell Lines ◽  
Endometrial Adenocarcinoma ◽  
Protein A ◽  
Rabbit Antiserum ◽  
Carcinoma Cells ◽  
Affinity Column ◽  
Antibody Technique ◽  
Galactosyl Transferase ◽  
C Terminus

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation.An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO2. The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.

Screening North American Plants In Vitro Against Leishmania Donovani, The Causative Agent For Visceral Leishmaniasis

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
SK Jain ◽  
MR Jacob ◽  
B Tekwani
Keyword(s):  
Visceral Leishmaniasis ◽  
Causative Agent ◽  
North American ◽  
Leishmania Donovani

Natural and synthetic indirubins as potent and selective inhibitors of the protozoan parasite Leishmania donovani

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
E Xingi ◽  
D Smirlis ◽  
S Bisti ◽  
V Myrianthopoulos ◽  
P Magiatis ◽  
...  
Keyword(s):  
Leishmania Donovani ◽  
Protozoan Parasite ◽  
Selective Inhibitors ◽  
Parasite Leishmania ◽  
Natural And Synthetic

IN VITRO DESTRUCTION OF LEISHMANIA DONOVANI INFECTED CELLS BY IMMUNE SERUM

1976 ◽  
Vol 4 (3-4) ◽  
pp. 207-211
Author(s):  
SHUN SHINBO ◽  
TAKATOSHI KOBAYAKAWA ◽  
HIROSHI ISHIYAMA ◽  
KAZUSHIGE MASUDA
Keyword(s):  
Leishmania Donovani ◽  
Immune Serum ◽  
Infected Cells

Ornithine decarboxylase of Leishmania donovani: Biochemical Properties and Possible Role of N-Terminal Extension

2015 ◽  
Vol 22 (2) ◽  
pp. 130-136 ◽  
Author(s):  
Mousumi Das ◽  
Ritesh Kumar ◽  
Vikash Dubey
Keyword(s):  
Ornithine Decarboxylase ◽  
Leishmania Donovani ◽  
Biochemical Properties
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