Abstract
Granulated bait formulations of Amdro, Award, and (S)-Methoprene were applied to individual RIFA mounds on 28 Aug 1996 in Collin Co., TX. Applications were applied to each mound individually with a hand-held shaker to distribute the bait evenly over an area ca. 3 ft in diam with the mound in the center. Plot size ranged from 300-500 ft2, each with 3 to 5 (mean = 3.7) active colonies. Plots were delineated with white turf marking paint; each colony within a plot was identified by numbers painted on the turf ca. 1.5 ft from the mound. Pre-treatment foraging activity was assayed by placing 3, 8-dram shell vial traps, baited with hot dog chips, near the center of each plot and no closer than 3 ft from an active colony. Vials were collected with RIFA foragers counted after 30 min exposure. Plots were divided into 4 replicates based on pre-treatment foraging numbers. RIFA foraging activity was assessed at 1, 2, 3, 5, and 7 wk after treatment (WAT). Individual mound mortality was determined at 1, 3, 5, and 7 WAT by stomping hard (4-5 times) on the soil or turf in a 1-ft circle around each mound. Upon disturbance, an active mound would yield many active workers. For rating at 4 and 6 wk, a hot dog chip was dropped upon the mound and observed 20-30 min later. If the colony was active, workers would forage the hot dog piece within this time period.