Detection of Retinoblastoma Gene Deletions in Spontaneous and Radiation-Induced Mouse Lung Adenocarcinomas by Polymerase Chain Reaction

1994 ◽  
Vol 137 (3) ◽  
pp. 310 ◽  
Author(s):  
Mark E. Churchill ◽  
M. Anne Gemmell ◽  
Gayle E. Woloschak
Keyword(s):  
Polymerase Chain Reaction ◽  
Mouse Lung ◽  
Retinoblastoma Gene ◽  
Chain Reaction ◽  
Gene Deletions ◽  
Radiation Induced ◽  
Polymerase Chain ◽  
Lung Adenocarcinomas

scholarly journals PCR detection of retinoblastoma gene deletions in radiation-induced mouse lung adenocarcinomas

1994 ◽  
Author(s):  
M.E. Churchill ◽  
M.A. Gemmell ◽  
G.E. Woloschak
Keyword(s):  
Pcr Detection ◽  
Mouse Lung ◽  
Retinoblastoma Gene ◽  
Gene Deletions ◽  
Radiation Induced ◽  
Lung Adenocarcinomas

Specific detection of mousepox virus by polymerase chain reaction

1997 ◽  
Vol 31 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Heinrich Neubauer ◽  
Martin Pfeffer ◽  
Hermann Meyer
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapid Identification ◽  
Mouse Lung ◽  
Chain Reaction ◽  
Base Pairs ◽  
Viral Origin ◽  
Body Protein ◽  
Polymerase Chain ◽  
Mouse Lung Tissue ◽  
Type Inclusion

Polymerase chain reaction was applied to the rapid identification and detection of mousepox virus. This was accomplished by selection of primers targeting the A-type inclusion body protein gene. By investigating 20 strains belonging to five different species of the genus Orthopoxvirus, amplification was achieved only with the seven mousepox virus strains examined. The size of the resulting DNA fragment accounted for 116 base pairs and contained a recognition site for the restriction enzyme HindII, thus confirming its viral origin. Amplification of mousepox virus specific sequences was also possible from infected mouse lung tissue and serum.

An Improved Polymerase Chain Reaction (PCR) Protocol for Unambigous Detection of Growth Hormone Gene Deletions

1998 ◽  
Vol 11 (4) ◽  
Author(s):  
C.M. Mone ◽  
V. Nigro ◽  
M. Rotondi ◽  
A. Del Buono ◽  
G. Mazziotti ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Polymerase Chain Reaction ◽  
Growth Hormone Gene ◽  
Chain Reaction ◽  
Gene Deletions ◽  
Polymerase Chain

Use of Polymerase Chain Reaction in Detection of Growth Hormone Gene Deletions*

1990 ◽  
Vol 70 (6) ◽  
pp. 1550-1553 ◽  
Author(s):  
CINDY L. VNENCAK-JONES ◽  
JOHN A. PHILLIPS ◽  
WANG DE-FEN
Keyword(s):  
Growth Hormone ◽  
Polymerase Chain Reaction ◽  
Growth Hormone Gene ◽  
Chain Reaction ◽  
Gene Deletions ◽  
Polymerase Chain

The use of deficiencies to determine essential gene content in the let-56–unc-22 region of Caenorhabditis elegans

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1148-1156 ◽  
Author(s):  
Jacquie E. Schein ◽  
Marco A. Marra ◽  
Guy M. Benian ◽  
Chris Fields ◽  
David L. Baillie
Keyword(s):  
Polymerase Chain Reaction ◽  
Caenorhabditis Elegans ◽  
Genomic Sequence ◽  
Essential Gene ◽  
Chain Reaction ◽  
Radiation Induced ◽  
Practical Tool ◽  
Polymerase Chain ◽  
Selection Of

We have investigated the possibility of using the polymerase chain reaction to detect deletions of coding elements in the unc-22–let-56 interval on chromosome IV in the nematode Caenorhabditis elegans. Our analysis of approximately 13 kb of genomic sequence immediately to the left of the unc-22 gene resulted in the identification of four possible genes. Partial cDNAs have been identified for three of them. To determine whether any of these coding elements are essential for development, we required a method for the induction and selection of mutations in these elements. Our approach was to identify a set of formaldehyde and gamma radiation induced unc-22 mutations that mapped to the unc-22–let-56 region, and then employ polymerase chain reaction methodology to identify deficiencies that affected one or more of the four identified coding elements. Two small deficiencies were identified in this manner. Characterization of these deficiencies shows that there are no coding elements between unc-22 and let-56 (the nearest mutationally identified gene to the left of unc-22), which are required in development under laboratory conditions. We conclude that the polymerase chain reaction is a practical tool for the detection of deletions of coding elements identified in this region, and that characterization of such deficiencies provides a method for assessing whether or not these elements are required for development.Key words: Caenorhabditis elegans, deficiencies, coding elements, unc-22.

Sign in / Sign up

Export Citation Format

Share Document