scholarly journals Three-Dimensional Shapes and Distributions of Long-Period Stacking Ordered Structures in Mg97Zn1Gd2 Cast Alloys Characterized by Electron Tomography

2015 ◽  
Vol 56 (7) ◽  
pp. 928-932 ◽  
Author(s):  
Kazuhisa Sato ◽  
Shuhei Matsunaga ◽  
Shunya Tashiro ◽  
Yohei Yamaguchi ◽  
Takanori Kiguchi ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Ordered Structures ◽  
Long Period ◽  
Cast Alloys

Evolution of long-period stacking order (LPSO) in Mg97Zn1Gd2 cast alloys viewed by HAADF-STEM multi-scale electron tomography

2018 ◽  
Vol 98 (21) ◽  
pp. 1945-1960 ◽  
Author(s):  
Kazuhisa Sato ◽  
Shunya Tashiro ◽  
Shuhei Matsunaga ◽  
Yohei Yamaguchi ◽  
Takanori Kiguchi ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Multi Scale ◽  
Long Period ◽  
Stacking Order ◽  
Cast Alloys

Practical electron tomography

1995 ◽  
Vol 53 ◽  
pp. 742-743
Author(s):  
C.L. Woodcock
Keyword(s):  
Electron Tomography ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3D Shape ◽  
Computational Resources ◽  
Shape Of Particles

Despite the potential of the technique, electron tomography has yet to be widely used by biologists. This is in part related to the rather daunting list of equipment and expertise that are required. Thanks to continuing advances in theory and instrumentation, tomography is now more feasible for the non-specialist. One barrier that has essentially disappeared is the expense of computational resources. In view of this progress, it is time to give more attention to practical issues that need to be considered when embarking on a tomographic project. The following recommendations and comments are derived from experience gained during two long-term collaborative projects.Tomographic reconstruction results in a three dimensional description of an individual EM specimen, most commonly a section, and is therefore applicable to problems in which ultrastructural details within the thickness of the specimen are obscured in single micrographs. Information that can be recovered using tomography includes the 3D shape of particles, and the arrangement and dispostion of overlapping fibrous and membranous structures.

Three-Dimensional reconstruction of isolated centrosomes by automated electron tomography

1994 ◽  
Vol 52 ◽  
pp. 310-311
Author(s):  
M.B. Braunfeld ◽  
M. Moritz ◽  
B.M. Alberts ◽  
J.W. Sedat ◽  
D.A. Agard
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Vital Role ◽  
Complex Nature ◽  
Animal Cells ◽  
Microtubule Organizing Center ◽  
Nucleation Sites ◽  
Dimensional Reconstruction ◽  
Organizing Center ◽  
Resolution Model

In animal cells, the centrosome functions as the primary microtubule organizing center (MTOC). As such the centrosome plays a vital role in determining a cell's shape, migration, and perhaps most importantly, its division. Despite the obvious importance of this organelle little is known about centrosomal regulation, duplication, or how it nucleates microtubules. Furthermore, no high resolution model for centrosomal structure exists.We have used automated electron tomography, and reconstruction techniques in an attempt to better understand the complex nature of the centrosome. Additionally we hope to identify nucleation sites for microtubule growth.Centrosomes were isolated from early Drosophila embryos. Briefly, after large organelles and debris from homogenized embryos were pelleted, the resulting supernatant was separated on a sucrose velocity gradient. Fractions were collected and assayed for centrosome-mediated microtubule -nucleating activity by incubating with fluorescently-labeled tubulin subunits. The resulting microtubule asters were then spun onto coverslips and viewed by fluorescence microscopy.

EMCAT: An automatic image-processing system for electron-microscopic tomography

1994 ◽  
Vol 52 ◽  
pp. 418-419
Author(s):  
Weiping Liu ◽  
John W. Sedat ◽  
David A. Agard
Keyword(s):  
Image Processing ◽  
Structural Information ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Processing System ◽  
Electron Microscopic ◽  
Data Set ◽  
Ordered Structures ◽  
Automatic Image Processing ◽  
Em Tomography

Any real world object is three-dimensional. The principle of tomography, which reconstructs the 3-D structure of an object from its 2-D projections of different view angles has found application in many disciplines. Electron Microscopic (EM) tomography on non-ordered structures (e.g., subcellular structures in biology and non-crystalline structures in material science) has been exercised sporadically in the last twenty years or so. As vital as is the 3-D structural information and with no existing alternative 3-D imaging technique to compete in its high resolution range, the technique to date remains the kingdom of a brave few. Its tedious tasks have been preventing it from being a routine tool. One keyword in promoting its popularity is automation: The data collection has been automated in our lab, which can routinely yield a data set of over 100 projections in the matter of a few hours. Now the image processing part is also automated. Such automations finish the job easier, faster and better.

Isolation Performance of Intelligent Seismic Isolation System Using Air Bearing

2009 ◽  
Author(s):  
Satoshi Fujita ◽  
Keisuke Minagawa ◽  
Mitsuru Miyazaki ◽  
Go Tanaka ◽  
Toshio Omi ◽  
...  
Keyword(s):  
Seismic Isolation ◽  
Seismic Waves ◽  
Three Dimensional ◽  
Vertical Motion ◽  
Air Bearings ◽  
Natural Period ◽  
Isolation System ◽  
Vertical Excitation ◽  
Long Period ◽  
Isolation Performance

This paper describes three-dimensional isolation performance of seismic isolation system using air bearings. Long period seismic waves having predominant period of from a few seconds to a few ten seconds have recently been observed in various earthquakes. Also resonances of high-rise buildings and sloshing of petroleum tanks in consequence of long period seismic waves have been reported. Therefore the isolation systems having very long natural period or no natural period are required. In a previous paper [1], we proposed an isolation system having no natural period by using air bearings. Additionally we have already reported an introduction of the system, and have investigated horizontal motion during earthquake in the previous paper. It was confirmed by horizontal vibration experiment and simulation in the previous paper that the proposed system had good performance of isolation. However vertical motion should be investigated, because vertical motion varies horizontal frictional force. Therefore this paper describes investigation regarding vertical motion of the proposed system by experiment. At first, a vertical excitation test of the system is carried out so as to investigate vertical dynamic property. Then a three-dimensional vibration test using seismic waves is carried out so as to investigate performance of isolation against three-dimensional seismic waves.

Three-Dimensional Nanoparticle Distribution and Local Curvature of Heterogeneous Catalysts Revealed by Electron Tomography

2007 ◽  
Vol 111 (31) ◽  
pp. 11501-11505 ◽  
Author(s):  
Edmund P. W. Ward ◽  
Timothy J. V. Yates ◽  
José-Jesús Fernández ◽  
David E. W. Vaughan ◽  
Paul A. Midgley
Keyword(s):  
Heterogeneous Catalysts ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Local Curvature ◽  
Nanoparticle Distribution

scholarly journals Capturing Enveloped Viruses on Affinity Grids for Downstream Cryo-Electron Microscopy Applications

2013 ◽  
Vol 20 (1) ◽  
pp. 164-174 ◽  
Author(s):  
Gabriella Kiss ◽  
Xuemin Chen ◽  
Melinda A. Brindley ◽  
Patricia Campbell ◽  
Claudio L. Afonso ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Measles Virus ◽  
Influenza A ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Nitrilotriacetic Acid ◽  
Dimensional Structure ◽  
Enveloped Viruses ◽  
Cryo Electron Microscopy ◽  
Human Immunodeficiency Virus Virus

AbstractElectron microscopy (EM), cryo-electron microscopy (cryo-EM), and cryo-electron tomography (cryo-ET) are essential techniques used for characterizing basic virus morphology and determining the three-dimensional structure of viruses. Enveloped viruses, which contain an outer lipoprotein coat, constitute the largest group of pathogenic viruses to humans. The purification of enveloped viruses from cell culture presents certain challenges. Specifically, the inclusion of host-membrane-derived vesicles, the complete destruction of the viruses, and the disruption of the internal architecture of individual virus particles. Here, we present a strategy for capturing enveloped viruses on affinity grids (AG) for use in both conventional EM and cryo-EM/ET applications. We examined the utility of AG for the selective capture of human immunodeficiency virus virus-like particles, influenza A, and measles virus. We applied nickel-nitrilotriacetic acid lipid layers in combination with molecular adaptors to selectively adhere the viruses to the AG surface. This further development of the AG method may prove essential for the gentle and selective purification of enveloped viruses directly onto EM grids for ultrastructural analyses.

The Prospect of Three-Dimensional Induction Mapping Inside Magnetic Nanostructures by Combining Electron Holography with Electron Tomography

2004 ◽  
Vol 10 (S02) ◽  
pp. 1010-1011 ◽  
Author(s):  
Rafal E Dunin-Borkowski ◽  
Takeshi Kasama
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Magnetic Nanostructures ◽  
Electron Holography

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

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