scholarly journals Long-term Clinical Observations of Periodontitis Patients Exhibited High Levels of Serum Antibody Titers to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis before Treatment.

1999 ◽  
Vol 41 (4) ◽  
pp. 461-468
Author(s):  
Takehisa Kojima ◽  
Yumiko Tominaga ◽  
Isao Ishikawa
Keyword(s):  
Porphyromonas Gingivalis ◽  
Serum Antibody ◽  
Actinobacillus Actinomycetemcomitans ◽  
Clinical Observations ◽  
Antibody Titers

scholarly journals A Novel Approach for Detecting an Immunodominant Antigen of Porphyromonas gingivalis in Diagnosis of Adult Periodontitis

1998 ◽  
Vol 5 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Toshihiasa Kawai ◽  
Hiro-O Ito ◽  
Nobuo Sakato ◽  
Hiroshi Okada
Keyword(s):  
Porphyromonas Gingivalis ◽  
Serum Antibody ◽  
Microscopic Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibody Response ◽  
Immunodominant Antigen ◽  
Crude Antigen ◽  
Novel Approach

ABSTRACT In the course of long-term infection with Porphyromonas gingivalis in adult periodontitis, a specific antibody response to this organism is generated. We describe a potential novel approach for identifying an immunodominant antigen in human periodontitis patients. First, various monoclonal antibodies (MAbs) were established from mice immunized with crude antigen preparations of P. gingivalis FDC 381. The antigen specificities of these MAbs were compared with those of serum antibodies of 10 periodontitis patients in a competitive enzyme-linked immunosorbent assay. The binding of one MAb (termed PF18) was readily inhibited by sera from all patients but not by sera from healthy volunteers. The antigen recognized by PF18 existed on the cell surface, presumably in the capsule layer, shown by immunoelectron microscopic analysis. Purification of the antigenic substance, termed PF18-Ag, was performed by immunoaffinity chromatography with the MAb. Characterization of PF18-Ag suggested that the epitope was composed of carbohydrates but not peptides and that the substance was different from lipopolysaccharide. Measurement of levels of serum antibody to PF18-Ag better discriminated periodontitis patients from healthy individuals than measurement of antibodies to crude antigen preparations of P. gingivalis. Immunoglobulin G2 was the predominant isotype among the antibodies to PF18-Ag in the patients’ sera. These results suggest that PF18-Ag, which is possibly a novel substance, is an important antigenic substance and is potentially useful for the clinical diagnosis of adult periodontitis. The approach that was used would also be relevant to detecting immunodominant antigens of other infectious microorganisms.

Long-term antibacterial activity of a composite coating on titanium for dental implant application

2020 ◽  
Vol 35 (6) ◽  
pp. 643-654
Author(s):  
Yicheng Cheng ◽  
Shenglin Mei ◽  
Xiangwei Kong ◽  
Xianghui Liu ◽  
Bo Gao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dental Implants ◽  
Composite Coating ◽  
Porphyromonas Gingivalis ◽  
Microarc Oxidation ◽  
Polymerase Chain Reaction Analysis ◽  
Titanium Implants ◽  
Actinobacillus Actinomycetemcomitans ◽  
Burst Release

Dental implants are the most innovative and superior treatment modality for tooth replacement. However, titanium implants still suffer from insufficient antibacterial capability and peri-implant diseases remain one of the most common and intractable complications. To prevent peri-implant diseases, a composite coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) was fabricated on titanium. This study was designed to investigate the antibacterial activity of the composite coating against two common peri-implant pathogens ( Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans). The morphology of the composite coating showed that BBF-loaded poly(L-lactic acid) nanospheres were well-distributed in the pores of the microarc oxidation coating, and cross-linked with each other and the wall pores by gelatin. A release study indicated that the antibacterial coating could sustain the release of BBF for 60 d, with a slight initial burst release occurring during the first 4 h. The antibacterial rate of the composite coating for adhering bacteria was the highest (over 97%) after 1 d and over 90% throughout a 30-day incubation period. The total fluorescence intensity of the composite coating was the lowest, and the vast majority of the fluorescence was red (dead bacteria). Moreover, real-time polymerase chain reaction analysis confirmed that the relative gene expression of the adherent bacteria on the composite coating was down-regulated. It was therefore concluded that the composite coating fabricated on titanium, which showed excellent and relatively long-term antibacterial activity against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, is a potential and promising strategy to be applied on dental implants for the prevention of peri-implant diseases.

Periodontal status and serum antibody titers for Porphyromonas gingivalis fimbriae in a rural population in Japan

2001 ◽  
Vol 28 (3) ◽  
pp. 264-269 ◽  
Author(s):  
Y. Furuichi ◽  
H.-O. Ito ◽  
Y. Izumi ◽  
T. Matsuyama ◽  
Y. Yotsumoto ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Rural Population ◽  
Serum Antibody ◽  
Periodontal Status ◽  
Antibody Titers

scholarly journals Aiolos Is Required for the Generation of High Affinity Bone Marrow Plasma Cells Responsible for Long-Term Immunity

2004 ◽  
Vol 199 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Marta Cortés ◽  
Katia Georgopoulos
Keyword(s):  
Bone Marrow ◽  
Plasma Cell ◽  
Plasma Cells ◽  
Somatic Hypermutation ◽  
Serum Antibody ◽  
High Affinity ◽  
Bone Marrow Plasma ◽  
Antibody Titers ◽  
Cell Defect

Antigenic encounter generates long-term immunity sustained by long-lived high affinity plasma cells resident in the bone marrow (BM). Here we show that the Ikaros family member, Aiolos, is specifically required for the generation of these plasma cells. Failure to generate high affinity plasma cells in the BM and to sustain serum antibody titers is apparent after both primary and secondary immunization of Aiolos−/− mice with a range of hapten concentrations. Chimera reconstitutions demonstrate that the BM plasma cell defect is B cell intrinsic. Lack of Aiolos does not alter expression of any of the previously described factors required for general plasma cell differentiation. No defect in somatic hypermutation, the generation of memory B cells, or short-lived high affinity plasma cells in the spleen was observed upon rechallenge. These studies support a model by which the high affinity plasma cell population in the BM undergoes a unique differentiation program that is dependent on Aiolos.

Биофармацевтические исследования по выбору основы для стоматологического геля с экстрактом барбариса

2020 ◽  
pp. 50-54
Author(s):  
З. Хаджиева ◽  
С. Поройский ◽  
И. Фирсова ◽  
А. Струсовская ◽  
В. Бавлакова ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Prevotella Intermedia ◽  
Prevotella Nigrescens

Исследованы биофармацевтические свойства модельных гелевых композиций c экстрактом корня барбариса и использованием в качестве гелеобразователей метилцеллюлозы, натриевой соли карбоксиметилцеллюлозы, натриевой соли альгиновой кислоты и поливинилового спирта методами прямой диффузии в агаровый гель и диализа через полупроницаемую мембрану. Определены кинетические закономерности процесса и скорости набухания исследуемых гелевых основ. Показана целесообразность использования натриевой соли альгиновой кислоты для получения стоматологического геля с экстрактом корня барбариса, обладающего специфической бактериостатической активностью в отношении таких пародонтопатогенных микроорганизмов, как Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens и Actinobacillus actinomycetemcomitans, для профилактики и лечения воспалительных заболеваний пародонта.

Generalized mycobacteriosis with secondary lesions of the spine in HIV positive patients

MedAlliance ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 54-60
Keyword(s):  
Antibiotic Therapy ◽  
Granulomatous Inflammation ◽  
Hiv Positive ◽  
Hiv Patients ◽  
Histological Picture ◽  
Clinical Observations ◽  
Spinal Lesion ◽  
Secondary Lesion

2 clinical observations of generalized forms of mycobacteriosis in HIV (+) patients complicated by secondary spinal lesion are described. Anamnestic data on the presence of mycobacteriosis of the lungs allowed to suspect a secondary lesion of the spine. Verification of the diagnosis is possible only with bacteriological confirmation, since the histological picture is nonspecific and resembles tuberculous granulomatous inflammation. Treatment of spondylitis caused by non-tuberculous mycobacteria is complex and includes both surgical rehabilitation of the focus and long-term (at least 1 year) antibiotic therapy.

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