Long-term antibacterial activity of a composite coating on titanium for dental implant application

2020 ◽  
Vol 35 (6) ◽  
pp. 643-654
Author(s):  
Yicheng Cheng ◽  
Shenglin Mei ◽  
Xiangwei Kong ◽  
Xianghui Liu ◽  
Bo Gao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dental Implants ◽  
Composite Coating ◽  
Porphyromonas Gingivalis ◽  
Microarc Oxidation ◽  
Polymerase Chain Reaction Analysis ◽  
Titanium Implants ◽  
Actinobacillus Actinomycetemcomitans ◽  
Burst Release

Dental implants are the most innovative and superior treatment modality for tooth replacement. However, titanium implants still suffer from insufficient antibacterial capability and peri-implant diseases remain one of the most common and intractable complications. To prevent peri-implant diseases, a composite coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) was fabricated on titanium. This study was designed to investigate the antibacterial activity of the composite coating against two common peri-implant pathogens ( Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans). The morphology of the composite coating showed that BBF-loaded poly(L-lactic acid) nanospheres were well-distributed in the pores of the microarc oxidation coating, and cross-linked with each other and the wall pores by gelatin. A release study indicated that the antibacterial coating could sustain the release of BBF for 60 d, with a slight initial burst release occurring during the first 4 h. The antibacterial rate of the composite coating for adhering bacteria was the highest (over 97%) after 1 d and over 90% throughout a 30-day incubation period. The total fluorescence intensity of the composite coating was the lowest, and the vast majority of the fluorescence was red (dead bacteria). Moreover, real-time polymerase chain reaction analysis confirmed that the relative gene expression of the adherent bacteria on the composite coating was down-regulated. It was therefore concluded that the composite coating fabricated on titanium, which showed excellent and relatively long-term antibacterial activity against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, is a potential and promising strategy to be applied on dental implants for the prevention of peri-implant diseases.

scholarly journals AntibacterialTiO2Coating Incorporating Silver Nanoparticles by Microarc Oxidation and Ion Implantation

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Zhang ◽  
Zhiguo Zhang ◽  
Wei Li
Keyword(s):  
Antibacterial Activity ◽  
Ion Implantation ◽  
Bacterial Colonization ◽  
Pure Titanium ◽  
Microarc Oxidation ◽  
Optimal Strategies ◽  
Titanium Implants ◽  
Silver Ion ◽  
Antibacterial Ability ◽  
Replacement Surgery

Infection associated with titanium implants remains the most common serious complication in hard tissue replacement surgery. Since such postoperative infections are usually difficult to cure, it is critical to find optimal strategies for preventing infections. In this study, TiO2coating incorporating silver (Ag) nanoparticles were fabricated on pure titanium by microarc oxidation and ion implantation. The antibacterial activity was evaluated by exposing the specimens toStaphylococcus aureusand comparing the reaction of the pathogens to Ti-MAO-Ag with Ti-MAO controls. Ti-MAO-Ag clearly inhibited bacterial colonization more than the control specimen. The coating’s antibacterial ability was enhanced by increasing the dose of silver ion implantation, and Ti-MAO-Ag20.0 had the best antibacterial ability. In addition, cytocompatibility was assessed by culturing cell colonies on the specimens. The cells grew well on both specimens. These findings indicate that surface modification by means of this process combining MAO and silver ion implantation is useful in providing antibacterial activity and exhibits cytocompatibility with titanium implants.

scholarly journals A Literature Review Study on Atomic Ions Dissolution of Titanium and Its Alloys in Implant Dentistry

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 368 ◽  
Author(s):  
Sammy Noumbissi ◽  
Antonio Scarano ◽  
Saurabh Gupta
Keyword(s):  
Dental Implants ◽  
Metal Ion ◽  
Titanium Implants ◽  
In Vitro Studies ◽  
Ion Release ◽  
Term Survival ◽  
Metal Ion Release

This review of literature paper was done in order to conduct a review of the literature and an assessment of the effects of titanium implant corrosion on peri-implant health and success in the oral environment. This paper evaluates and critically reviews the findings of the multiple in-depth in vivo and in vitro studies that are related to corrosion aspects of the titanium and its alloys. A literature survey was conducted by electronic search in Medline and studies that were published between 1940 and August 2018 were selected. The search terms used were types of corrosion, corrosion of titanium implants, titanium corrosion, metal ion release from the titanium implants, fretting and pitting corrosion, implant corrosion, peri implantitis, and corrosion. Both in vivo and in vitro studies were also included in the review. The search and selection resulted in 64 articles. These articles were divided on the basis of their context to different kinds of corrosion related to titanium dental implants. It is evident that metal ions are released from titanium and titanium alloy dental implants as a result of corrosion. Corrosion of implants is multifactorial, including electrical, chemical, and mechanical factors, which have an effect on the peri-implant tissues and microbiota. The literature surveyed showed that corrosion related to titanium and its alloys has an effect on the health of peri-implant soft and hard tissue and the long term survival of metal dental implants. It can be concluded that presence of the long-term corrosion reaction along with continuous corrosion leads to the release of ions into the peri-implant tissue but also to a disintegration of the implant that contribute to material fatigue and even fracture of the abutments and implant body or both. This combined impact of the corrosion, bacterial activity, chemical reactions, and functional stresses are to be looked at as important factors of implant failure. The findings can be used to explore the possible strategies of research to investigate the biological impact of implant materials.

scholarly journals Elevated Systemic L-Kynurenine/L-Tryptophan Ratio and Increased IL-1 Beta and Chemokine (CX3CL1, MCP-1) Proinflammatory Mediators in Patients with Long-Term Titanium Dental Implants

2019 ◽  
Vol 8 (9) ◽  
pp. 1368 ◽  
Author(s):  
José Joaquín Merino ◽  
María Eugenia Cabaña-Muñoz ◽  
Adolfo Toledano Gasca ◽  
Alba Garcimartín ◽  
Juana Benedí ◽  
...  
Keyword(s):  
Dental Implants ◽  
Dental Materials ◽  
Study Groups ◽  
Dental Amalgam ◽  
Titanium Implants ◽  
Monocyte Chemoattractant Protein 1 ◽  
Proinflammatory Mediators ◽  
Dental Amalgam Fillings ◽  
Titanium Dental Implants

Titanium is the mean biocompatible metal found in dental titanium alloys (Ti-6Al-4V). The safety of certain dental biomaterial amalgams has been questioned in patients. The levels of several systemic cytokines (interleukin (IL)-1 beta, IL-4: pg/mL) and chemokines (monocyte chemoattractant protein-1 (MCP-1), soluble fractalkine (CX3CL1: pg/mL) were determined using ELISA and compared between these study groups. The study included 30 controls without dental materials (cont), 57 patients with long-term titanium dental implants plus amalgams (A + I group) as well as 55 patients with long-term dental amalgam alone (A group). All patients (except controls) have had dental titanium implants (Ti-6Al-4V) and/or amalgams for at least 10 years (average: 15 years). We evaluated whether systemic levels of cytokines/chemokines, kyn/L-trp ratio and aromatic amino acid levels (HPLC: mM/L, Phe, L-Trp, His, Treo) could be altered in patients with long-term dental titanium and/or amalgams. These systemic markers were evaluated in 142 patients. The A + I group had higher L-Kynurenine/L-Tryptophan ratios than patients with long-term dental amalgam fillings alone (A). In addition, levels of IL-1 Beta cytokine, CX3CL1 and MCP-1 chemokines were higher in the A + I group than in the A group (A). The increased L-kyn/L-trp ratio and MCP-1 and fractalkine receptor (CX3CR1) elevations could suggest enhanced chemotactic responses by these chemokines in the A + I group.

scholarly journals Increased Systemic Malondialdehyde Levels and Decreased Mo/Co, Mo/Hg2+, Co/Fe2+ Ratios in Patients with Long-Term Dental Titanium Implants and Amalgams

2019 ◽  
Vol 8 (1) ◽  
pp. 86 ◽  
Author(s):  
María Cabaña-Muñoz ◽  
José Parmigiani-Izquierdo ◽  
Fabio Camacho Alonso ◽  
José Merino
Keyword(s):  
Oxidative Stress ◽  
Dental Implants ◽  
Dental Materials ◽  
Dental Amalgam ◽  
Titanium Implants ◽  
Systemic Oxidative Stress ◽  
Hair Samples ◽  
Dental Amalgams ◽  
Titanium Dental Implants

Introduction: the biological safety of dental biomaterials has been questioned in human studies. Material and Methods: Several heavy metals/oligoelements were compared by Inductive Coupled Mass Spectrometry (ICP-MS) in hair samples from 130 patients (n = 54 patients with long-term titanium dental implants and amalgams (A + I group), 51 patients with long-term dental amalgam alone (A group), as well as controls (n = 25: without dental materials) of similar age. All patients (except controls) had had titanium dental implants and/or dental amalgams for at least 10 years (average: 17). We evaluated whether A + I patients could present higher systemic malondialdehyde levels (MDA) as compared to the A group. Results: The A + I group have lower molybdenum levels (A + I) and reduced Mo/Co and Mo/Fe2+ ratios, which could predispose them to oxidative stress by raising MDA levels as compared to the A group alone; our findings suggest that higher Co levels could enhance oxidative stress in the A + I group. However, there were no differences on metals from titanium alloy (Ti-6Al), Cr from crowns or Hg2+, Sn, Zn2+, Cu2+ levels between the A + I and A groups. Conclusion: patients with long-term dental titanium and amalgams have systemic oxidative stress due to rising MDA levels and lower Mo/Co and Mo/Fe2+ ratios than those with amalgams alone.

scholarly journals Mobility of B.C.T. Dental implants inserted by one and two-phase surgical method: An experimental study

2006 ◽  
Vol 63 (10) ◽  
pp. 867-872
Author(s):  
Novak Stamatovic ◽  
Smiljana Matic ◽  
Zoran Lazic ◽  
Zoran Tatic
Keyword(s):  
Dental Implants ◽  
Titanium Implants ◽  
Phase Method ◽  
Average Weight ◽  
Crucial Importance ◽  
Two Phase ◽  
Term Survival ◽  
Surgical Method ◽  
The Difference

Background/Aim. Achievement of the osseointegration of dental implants is of crucial importance for their long-term survival. One of the factors that influence the osseointegration is a surgical method of implantation. The outcome of osseointegration can be evaluated on the basis of implant mobility in bone. The aim of this study was to investigate and compare the mobility of B.C.T. dental titanium implants inserted to experimental animals using an one and two-phase method. Methods. The investigation was performed using a split-mouth design on nine dogs, male german shephards, average age of 3.5 years and average weight of 32 kg. Extractions of the third and fourth lower premolars were performed under intravenous (i.v.) anaesthesia with 5% ketamine chloride. Eight weeks after the extractions, the implants, diameter of 4.5 mm each, with four threads 13.7 mm long, were inserted. Eighteen implants were inserted one side of the jaw using a one-phase method, and another 18 implants were inserted contralaterally using a two-phase method. Three months after the implantation, the implant mobility was evaluated. Three measurements were performed with a Periotest device, and average values were calculated. The implant mobility was classified according to the Periotest scale in four groups of Periotest values (PTV) and compared. A total of 36 implants were inserted in 9 experimental dogs. The PTV ranged from -7.666 to + 50. Results. According to the Periotest scale, 14 one-phase implants (78%) were classified into the 0 group of PTV, and 4 one-phase implants (22%) in the 3rd group. Thirteen two phase implants (72%) were classified in the 0 group, and 5 implants in the 3rd group of Periotest scale. The difference in the average values of PTV between the two methods was 0.879 which was not statistically significant. Conclusion. This study showed that the method of implant insertion had no influence on the implant mobility, i.e. satisfactory osseointegration could be achieved by both methods. Further clinical parameters, as well as pathohistological and histomorphometric ones, have to be evaluated in order to assess better outcome of a particular method.

scholarly journals Antibacterial Activity against Staphylococcus Aureus of Titanium Surfaces Coated with Graphene Nanoplatelets to Prevent Peri-Implant Diseases. An In-Vitro Pilot Study

2020 ◽  
Vol 17 (5) ◽  
pp. 1568 ◽  
Author(s):  
Nicola Pranno ◽  
Gerardo La Monaca ◽  
Antonella Polimeni ◽  
Maria Sabrina Sarto ◽  
Daniela Uccelletti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pilot Study ◽  
Dental Implants ◽  
Graphene Nanoplatelets ◽  
Titanium Implants ◽  
Titanium Surfaces ◽  
Biofilm Development ◽  
Titanium Disk

Dental implants are one of the most commonly used ways to replace missing teeth. Nevertheless, the close contact with hard and soft oral tissues expose these devices to infectious peri-implant diseases. To prevent such infection, several surface treatments have been developed in the last few years to improve the antimicrobial properties of titanium dental implants. In this in-vitro pilot study, the antimicrobial activity of titanium surfaces coated with different types of graphene nanoplatelets are investigated. Six different colloidal suspensions of graphene nanoplatelets (GNPs) were produced from graphite intercalated compounds, setting the temperature and duration of the thermal shock and varying the number of the exfoliation cycles. Titanium disks with sand-blasted and acid-etched surfaces were sprayed with 2 mL of colloidal GNPs suspensions. The size of the GNPs and the percentage of titanium disk surfaces coated by GNPs were evaluated through a field emission-scanning electron microscope. The antibacterial activity of the specimens against Staphylococcus aureus was estimated using a crystal violet assay. The dimension of GNPs decreased progressively after each sonication cycle. The two best mean percentages of titanium disk surfaces coated by GNPs were GNPs1050°/2 and GNPs1150°/2. The reduction of biofilm development was 14.4% in GNPs1150°/2, 20.1% in GNPs1150°/3, 30.3% in GNPs1050°/3, and 39.2% in GNPs1050°/2. The results of the study suggested that the surface treatment of titanium disks with GNPs represents a promising solution to improve the antibacterial activity of titanium implants.

The Effect of Microarc Oxidation Ceramic Coating on Sensory Reconstruction around Implants Using Cultured Schwann Cells

2007 ◽  
Vol 342-343 ◽  
pp. 697-700
Author(s):  
Quan Yuan ◽  
Zhen Tan ◽  
Ping Gong ◽  
Rong Rong Nie ◽  
Xiao Yu Li ◽  
...  
Keyword(s):  
Dental Implants ◽  
Schwann Cells ◽  
Ceramic Coating ◽  
Pure Titanium ◽  
Microarc Oxidation ◽  
Titanium Implants ◽  
Porous Coating ◽  
Enzyme Linked Immunosorbent Assay ◽  
Total Protein Content ◽  
Sensory Reconstruction

Osseointegrated titanium implants have been widely used in clinics for replacement of missing teeth. However, sensory perception thresholds of implants were 10 to 100 times higher than those of natural teeth. The purpose of this study was to evaluate the effect of the ceramic coating generated by microarc oxidation (MAO) on sensory reconstruction around dental implants. The MAO coating was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and scanning probe microscope (SPM). Moreover, the chemical composition was analyzed with an energy dispersive spectroscope (EDS). Using smooth titanium discs as a control, Schwann cell responses to the coating were evaluated by SEM, MTT assay, total protein content and leakage of cytosolic lactate dehydrogenase (LDH) activity. In addition, the amount of brain-derived neurotrophic factor (BDNF) secreted by Schwann cells was measured by Enzyme-Linked Immunosorbent Assay (ELISA). It was observed that the porous coating had separated and homogeneous micropores in the diameter of 1-2 μm. The concentrations of Ca and P in the layer were 8.63% and 5.23% respectively. The cell morphology, proliferation and secretion of BDNF were unaffected. The results indicated that the MAO ceramic coating developed on the surface of pure titanium had good biocompatibility with Schwann cells for sensory reconstruction around dental implants.

Long-Term Study of Dental Implants Placed into Alveolar Cleft Sites

2007 ◽  
Vol 44 (4) ◽  
pp. 444-447 ◽  
Author(s):  
Yoshiro Matsui ◽  
Kohsuke Ohno ◽  
Akiko Nishimura ◽  
Tatsuo Shirota ◽  
Syutaku Kim ◽  
...  
Keyword(s):  
Dental Implants ◽  
Bone Grafting ◽  
Survival Rates ◽  
Surface Characteristics ◽  
Titanium Implants ◽  
First Year ◽  
Marginal Bone Loss ◽  
Long Term Study

Objective: To evaluate the long-term prognosis of dental implants placed into cleft sites after bone grafting in a relatively large number of cases in order to clarify the usefulness of the modality. Patients: Forty-seven patients with unilateral (dental, n = 32) or bilateral (n = 15) clefts of the alveolar process were included in this study. Interventions: A total of 71 implants, including smooth- or rough-surface titanium, and hydroxyapatite (HA)-coated implants, were placed after bone grafting from the anterior iliac crest and/or mandible. The placed implants were 13 to 15 mm in length. Main outcome: The follow-up period was from 21 to 120 months (average = 60 months). Implant survival rates were calculated as cumulative survival. Marginal bone loss (MBL) from the implant shoulder was examined, with statistical analyses performed on the influence of simultaneous bone graft and surface characteristics. Results: At the end of the clinical follow-up period, all implants except one were in situ and stable. Thus, the overall survival rate was 98.6% at the end of the first year and remained the same until the end of observation. Titanium implants with smooth surfaces had the lowest MBL with almost negligible regression slope, although some implants had relatively high MBL by the end of the first year. Conclusions: Implant therapy in the cleft site offers a reliable option for patients. Particular attention should be focused preoperatively on whether bone volume can provide primary implant stability.

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