E6 and E7 oncogene expression by human papilloma virus (HPV) and the aggressive behavior of recurrent laryngeal papillomatosis (RLP)

2006 ◽  
Vol preprint (2007) ◽  
pp. 1
Author(s):  
Bahig Shehata ◽  
Kristen Otto ◽  
Steven Sobol ◽  
Christina Stockwell ◽  
Cora Foulks ◽  
...  
Keyword(s):  
Human Papilloma Virus ◽  
Aggressive Behavior ◽  
Laryngeal Papillomatosis ◽  
Papilloma Virus ◽  
Oncogene Expression ◽  
E6 And E7 ◽  
E7 Oncogene

E6 and E7 Oncogene Expression by Human Papilloma Virus (HPV) and the Aggressive Behavior of Recurrent Laryngeal Papillomatosis (RLP)

2008 ◽  
Vol 11 (2) ◽  
pp. 118-121 ◽  
Author(s):  
Bahig M. Shehata ◽  
Kristen J. Otto ◽  
Steven E. Sobol ◽  
Christina A. Stockwell ◽  
Cora Foulks ◽  
...  
Keyword(s):  
Human Papilloma Virus ◽  
Surgical Procedures ◽  
Clinical Course ◽  
Disease Onset ◽  
Malignant Degeneration ◽  
Laryngeal Papillomatosis ◽  
Papilloma Virus ◽  
Oncogene Expression ◽  
E6 And E7 ◽  
E7 Oncogene

Recurrent laryngeal papillomatosis (RLP), a chronic disease associated with human papilloma virus (HPV), requires serial surgical procedures for debulking, resulting in debilitating long-term dysphonia, laryngeal scarring, and rarely malignant degeneration. Human papilloma virus 11 tumors have been widely accepted as more aggressive than HPV 6 tumors; however, the clinical course has been difficult to predict at disease onset, and the biologic mediators of proliferation have not been well characterized. A retrospective case review of 43 patients (4 months to 10 years at diagnosis) was performed on children treated for recurrent laryngeal papillomatosis. Patient charts were reviewed for demographic information, age at RLP diagnosis, approximate frequency of surgical intervention, and absolute number of surgical procedures performed. Human papilloma virus subtyping was performed. Expression analysis of the HPV-encoded E6 and E7 oncogenes was performed by reverse-transcriptase polymerase chain reaction. Fourteen patients had subtype 11 (33%) and 29 patients had subtype 6 (67%). As expected, HPV 11 patients showed a more aggressive clinical course than HPV 6 patients. However, 38% of patients with subtype 6 (11 patients) followed a clinical course that mirrored the more severe subtype 11 patients. These patients expressed the disease at a younger age ( P < 0.0002) and showed higher levels of E6 and E7 oncogenes compared to the patients with the more indolent course. Although HPV subtype and early onset of RLP are well characterized prognostic factors, our study documents the significance of E6 and E7 oncogene expression as potential biologic mediators of proliferation and thereby clinical behavior.

Construction and characterization of recombinant fowlpox viruses expressing human papilloma virus E6 and E7 oncoproteins

2009 ◽  
Vol 158 (1-2) ◽  
pp. 184-189 ◽  
Author(s):  
Eleana Pozzi ◽  
Valeria Basavecchia ◽  
Carlo Zanotto ◽  
Sole Pacchioni ◽  
Carlo De Giuli Morghen ◽  
...  
Keyword(s):  
Human Papilloma Virus ◽  
Papilloma Virus ◽  
E6 And E7 Oncoproteins ◽  
E6 And E7

scholarly journals Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins

Oncotarget ◽  
2017 ◽  
Vol 8 (42) ◽  
pp. 72666-72675 ◽  
Author(s):  
Vladimir A. Mitkevich ◽  
Ksenia M. Burnysheva ◽  
Irina Yu Petrushanko ◽  
Alexei A. Adzhubei ◽  
Alexey A. Schulga ◽  
...  
Keyword(s):  
Human Papilloma Virus ◽  
Cervical Carcinoma ◽  
Carcinoma Cells ◽  
Papilloma Virus ◽  
E6 And E7

scholarly journals Involvement of p53-dependent apoptosis signal in antitumor effect of Colchicine on human papilloma virus (HPV)-positive human cervical cancer cells

2020 ◽  
Vol 40 (3) ◽  
Author(s):  
Luchun Yan ◽  
Hao Huang ◽  
Ying Zhang ◽  
Xinrong Yuan ◽  
Zhaohua Yan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papilloma Virus ◽  
Colchicine Treatment ◽  
Inhibitory Effect ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Papilloma Virus ◽  
Treatment And Prevention ◽  
E6 And E7 ◽  
Human Cervical Cancer

Abstract Colchicine, a plant-derived alkaloid with relatively low toxicity on normal human epidermal keratinocytes (HEKn), has selective inhibitory effect on the growth of CaSki (HPV16-positive) and HeLa (HPV18-positive) human cervical cancer cell lines via the induction of apoptosis. Colchicine (2.5, 5.0 and 10.0 ng/ml) significantly reduced the expression of human papilloma virus (HPV) 16 E6/E7 mRNA and protein in CaSki and HeLa cells. Moreover, reduced expression of E6 and E7 induced by Colchicine resulted in the up-regulation of tumor suppressor proteins, p53 and Rb, as well as down-regulation of phospho Rb (pRb) protein. In addition, Bax, cytosolic cytochrome c and cleaved caspase-3 protein were increased while Bcl-2 protein was decreased significantly by 48 h of Colchicine treatment. These results implied that Colchicine could be explored as a potent candidate agent for the treatment and prevention of HPV-associated cervical cancer without deleterious effects.

A Comprehensive Review on Crosstalk of Human Papilloma Virus oncoproteins and developmental/self-renewal pathways during the pathogenesis of uterine cervical cancer

2020 ◽  
Vol 20 ◽  
Author(s):  
Afza Ahmad ◽  
Irfan Ahmad Ansari
Keyword(s):  
Cervical Cancer ◽  
Human Papilloma Virus ◽  
Epithelial Mesenchymal Transition ◽  
Wnt Signaling Pathway ◽  
Hpv Infection ◽  
Uterine Cervical Cancer ◽  
Self Renewal ◽  
Papilloma Virus ◽  
Mesenchymal Transition ◽  
E6 And E7

: Cervical cancer, a cancer arising from the uterine cervix has been regarded as the fourth most frequent gynecological malignancy among females worldwide. Epidemiological reports have shown that uterine cervical cancer is a global health issue among womens of specially developing countries and consequently creates an economic and medical burden in the society. The main causative agent of cervical carcinoma is high risk human papilloma virus (HPV 16 and HPV 18). Molecular studies have revealed the expression two viral genes E6 and E7 after HPV infection in the epithelial cells of cervix. These gene products are known to inactivate the major tumor suppressors, p53 and retinoblastoma protein (pRB), respectively. Moreover, the role of self-renewal pathways such as Hedgehog, Notch and Wnt has also been linked with drug resistance in cancer cells and epithelial mesenchymal transition during metastasis in pathogenesis of cervical cancer. Although, the mechanism of interaction of HPV E6 and E7 with each and every component of above described developmental pathways is not elucidated yet, but preliminary reports of their crosstalk have begun to emerge. Understanding the interplay between these oncoproteins and developmental/self-renewal pathways is highly important in terms of designing new and targeted therapeutic approach against cervical cancer. Hence, this review cynosure the carcinogenesis of HPV with the brief description of its virology and also establishes the crosstalk between oncoproteins E6 & E7 and Hedgehog, Notch and Wnt signaling pathway.

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