Influence of different concentrations of fibrinogen on the properties of a fibrin matrix for vascular tissue engineering

2021 ◽  
Vol 29 (1) ◽  
pp. 21-34
Author(s):  
Vera G. Matveeva ◽  
Mariam Yu. Khanova ◽  
Tatyana V. Glushkova ◽  
Larisa V. Antonova
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Metabolic Activity ◽  
Vascular Tissue ◽  
Physical And Mechanical Properties ◽  
Vascular Tissue Engineering ◽  
Test Machine ◽  
Fibrin Matrix ◽  
Before And After ◽  
The Difference

Aim. To evaluate the potential utility of fibrin matrices containing 10, 20, and 25 mg/ml of fibrinogen (fibrin-10, fibrin-20, and fibrin-30, respectively) in vascular tissue engineering (VTE). Materials and Methods. Fibrinogen was isolated using the method of ethanol cryoprecipitation and polymerized using a solution of thrombin and CaCl2. The fibrin structure was studied in a scanning electron microscope, and the physical and mechanical properties of the material were tested on a Zwick/Roell test machine. The metabolic activity of endothelial cells (EC) on the fibrin surface was evaluated by the MTT assay, and the viability of fibroblasts in the thickness of fibrin and possibility for migration by in fluorescent and light microscopy. Percent of fibrin shrinkage was determined from the difference in the sample volumes before and after removal of moisture. Results. The fiber diameter did not differ among all fibrin samples, but the pore diameter in fibrin-30 was smaller than those in fibrin-10 and fibrin-20. A possibility for migration of fibroblasts into the depth of the fibrin matrix and preservation of 97-100% viability of cells at a depth 5 mm was confirmed. The metabolic activity of EC on the surface of fibrin-20 and fibrin-30 exceeded that on collagen, fibronectin, and fibrin-10. All fibrin samples shrank in volume to 95.5-99.5%, and the highest shrinkage was seen in fibrin-10. The physical and mechanical properties of fibrin were inferior to those of human A. mammaria by a factor of 10. Conclusion. Fibrin with fibrinogen concentrations of 20 and 30 mg/ml maintains a high metabolic and proliferative activity of EC on the surface and also a high viability of fibroblasts in the matrix. Its availability, ease of preparation, and a number of other favorable properties make fibrin a promising material for VTE. However, the problem of insufficient strength requires further investigations.

scholarly journals Erratum to “Detergent-Enzymatic Decellularization of Swine Blood Vessels: Insight on Mechanical Properties for Vascular Tissue Engineering”

2014 ◽  
Vol 2014 ◽  
pp. 1-2 ◽  
Author(s):  
Alessandro F. Pellegata ◽  
M. Adelaide Asnaghi ◽  
Ilaria Stefani ◽  
Anna Maestroni ◽  
Silvia Maestroni ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering

scholarly journals Mathematical Modeling of Uniaxial Mechanical Properties of Collagen Gel Scaffolds for Vascular Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ramiro M. Irastorza ◽  
Bernard Drouin ◽  
Eugenia Blangino ◽  
Diego Mantovani
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Vascular Tissue ◽  
Nonlinear Models ◽  
Small Diameter ◽  
Collagen Gel ◽  
Vascular Tissue Engineering ◽  
Suitable Model ◽  
Collagen Gels ◽  
Hammerstein Models

Small diameter tissue-engineered arteries improve their mechanical and functional properties when they are mechanically stimulated. Applying a suitable stress and/or strain with or without a cycle to the scaffolds and cells during the culturing process resides in our ability to generate a suitable mechanical model. Collagen gel is one of the most used scaffolds in vascular tissue engineering, mainly because it is the principal constituent of the extracellular matrix for vascular cells in human. The mechanical modeling of such a material is not a trivial task, mainly for its viscoelastic nature. Computational and experimental methods for developing a suitable model for collagen gels are of primary importance for the field. In this research, we focused on mechanical properties of collagen gels under unconfined compression. First, mechanical viscoelastic models are discussed and framed in the control system theory. Second, models are fitted using system identification. Several models are evaluated and two nonlinear models are proposed: Mooney-Rivlin inspired and Hammerstein models. The results suggest that Mooney-Rivlin and Hammerstein models succeed in describing the mechanical behavior of collagen gels for cyclic tests on scaffolds (with best fitting parameters 58.3% and 75.8%, resp.). When Akaike criterion is used, the best is the Mooney-Rivlin inspired model.

scholarly journals Detergent-Enzymatic Decellularization of Swine Blood Vessels: Insight on Mechanical Properties for Vascular Tissue Engineering

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Alessandro F. Pellegata ◽  
M. Adelaide Asnaghi ◽  
Ilaria Stefani ◽  
Anna Maestroni ◽  
Silvia Maestroni ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Vascular Tissue ◽  
Complete Removal ◽  
Vascular Tissue Engineering ◽  
Promising Tool ◽  
Complete Cell ◽  
Synthetic Grafts

Small caliber vessels substitutes still remain an unmet clinical need; few autologous substitutes are available, while synthetic grafts show insufficient patency in the long term. Decellularization is the complete removal of all cellular and nuclear matters from a tissue while leaving a preserved extracellular matrix representing a promising tool for the generation of acellular scaffolds for tissue engineering, already used for various tissues with positive outcomes. The aim of this work is to investigate the effect of a detergent-enzymatic decellularization protocol on swine arteries in terms of cell removal, extracellular matrix preservation, and mechanical properties. Furthermore, the effect of storage at −80°C on the mechanical properties of the tissue is evaluated. Swine arteries were harvested, frozen, and decellularized; histological analysis revealed complete cell removal and preserved extracellular matrix. Furthermore, the residual DNA content in decellularized tissues was far low compared to native one. Mechanical testings were performed on native, defrozen, and decellularized tissues; no statistically significant differences were reported for Young’s modulus, ultimate stress, compliance, burst pressure, and suture retention strength, while ultimate strain and stress relaxation of decellularized vessels were significantly different from the native ones. Considering the overall results, the process was confirmed to be suitable for the generation of acellular scaffolds for vascular tissue engineering.

scholarly journals Biomechanically Tunable Nano-Silica/P-HEMA Structural Hydrogels for Bone Scaffolding

2021 ◽  
Author(s):  
Raffaella Aversa ◽  
Relly Victoria Petrescu ◽  
Florian Ion T. Petrescu ◽  
Davide Apicella ◽  
and Antonio Apicella
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bulk Modulus ◽  
Physical And Mechanical Properties ◽  
Sorption Kinetics ◽  
Swelling Kinetics ◽  
Swelling Rate ◽  
Biomimetic Scaffold ◽  
The Difference ◽  
Saline Solutions

Innovative tissue engineering biomimetic hydrogels based on hydrophilic polymers have been investigated for their physical and mechanical properties. 5% to 25% by volume loading PHEMA-nanosilica glassy hybrid samples were equilibrated at 37°C in aqueous physiological isotonic and hypotonic saline solutions (0.15 and 0.05 M NaCl) simulating two limiting possible compositions of physiological extracellular fluids. The glassy and hydrated hybrid materials were characterized both for dynamo-mechanical properties and equilibrium absorptions in the two physiological-like aqueous solutions. Mechanical and the morphological modifications occurring in the samples have been described. The 5% volume nanosilica loading hybrid nanocomposite composition showed mechanical characteristics in the dry and hydrated states that were comparable to those of cortical bone and articular cartilage, respectively, and then chosen for further sorption kinetics characterization. Sorption and swelling kinetics were monitored up to equilibrium. Changes in water activities and osmotic pressures in the water-hybrid systems equilibrated at the two limiting solute molarities of the physiological solutions have been related to the observed anomalous sorption modes using the Flory-Huggins interaction parameter approach. The bulk modulus of the dry and glassy PHEMA-5% nanosilica hybrid at 37°C has been observed to be comparable with the values of the osmotic pressures generated from the sorption of isotonic and hypotonic solutions. The anomalous sorption modes and swelling rates are coherent with the difference between osmotic swelling pressures and hybrid glassy nano-composite bulk modulus: the lower the differences the higher the swelling rate and equilibrium solution uptakes. Bone tissue engineering benefits of use of tuneable biomimetic scaffold biomaterials that can be “designed” to act as biocompatible and biomechanically active hybrid interfaces are discussed.

scholarly journals Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Sébastien Meghezi ◽  
Frédéric Couet ◽  
Pascale Chevallier ◽  
Diego Mantovani
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Viscoelastic Properties ◽  
Saline Solution ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Tensile Tests ◽  
Biological Properties ◽  
Vascular Tissue Engineering ◽  
Collagen Gels

Vascular tissue engineering focuses on the replacement of diseased small-diameter blood vessels with a diameter less than 6 mm for which adequate substitutes still do not exist. One approach to vascular tissue engineering is to culture vascular cells on a scaffold in a bioreactor. The bioreactor establishes pseudophysiological conditions for culture (medium culture, 37°C, mechanical stimulation). Collagen gels are widely used as scaffolds for tissue regeneration due to their biological properties; however, they exhibit low mechanical properties. Mechanical characterization of these scaffolds requires establishing the conditions of testing in regard to the conditions set in the bioreactor. The effects of different parameters used during mechanical testing on the collagen gels were evaluated in terms of mechanical and viscoelastic properties. Thus, a factorial experiment was adopted, and three relevant factors were considered: temperature (23°C or 37°C), hydration (aqueous saline solution or air), and mechanical preconditioning (with or without). Statistical analyses showed significant effects of these factors on the mechanical properties which were assessed by tensile tests as well as stress relaxation tests. The last tests provide a more consistent understanding of the gels' viscoelastic properties. Therefore, performing mechanical analyses on hydrogels requires setting an adequate environment in terms of temperature and aqueous saline solution as well as choosing the adequate test.

scholarly journals Biomechanically Tunable Nano-Silica/P-HEMA Structural Hydrogels for Bone Scaffolding

2021 ◽  
Vol 8 (4) ◽  
pp. 45
Author(s):  
Raffaella Aversa ◽  
Relly Victoria Petrescu ◽  
Florian Ion T. Petrescu ◽  
Valeria Perrotta ◽  
Davide Apicella ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bulk Modulus ◽  
Physical And Mechanical Properties ◽  
Sorption Kinetics ◽  
Swelling Kinetics ◽  
Swelling Rate ◽  
Biomimetic Scaffold ◽  
The Difference ◽  
Saline Solutions

Innovative tissue engineering biomimetic hydrogels based on hydrophilic polymers have been investigated for their physical and mechanical properties. 5% to 25% by volume loading PHEMA-nanosilica glassy hybrid samples were equilibrated at 37 °C in aqueous physiological isotonic and hypotonic saline solutions (0.15 and 0.05 M NaCl) simulating two limiting possible compositions of physiological extracellular fluids. The glassy and hydrated hybrid materials were characterized by both dynamo-mechanical properties and equilibrium absorptions in the two physiological-like aqueous solutions. The mechanical and morphological modifications occurring in the samples have been described. The 5% volume nanosilica loading hybrid nanocomposite composition showed mechanical characteristics in the dry and hydrated states that were comparable to those of cortical bone and articular cartilage, respectively, and then chosen for further sorption kinetics characterization. Sorption and swelling kinetics were monitored up to equilibrium. Changes in water activities and osmotic pressures in the water-hybrid systems equilibrated at the two limiting solute molarities of the physiological solutions have been related to the observed anomalous sorption modes using the Flory-Huggins interaction parameter approach. The bulk modulus of the dry and glassy PHEMA-5% nanosilica hybrid at 37 °C has been observed to be comparable with the values of the osmotic pressures generated from the sorption of isotonic and hypotonic solutions. The anomalous sorption modes and swelling rates are coherent with the difference between osmotic swelling pressures and hybrid glassy nano-composite bulk modulus: the lower the differences the higher the swelling rate and equilibrium solution uptakes. Bone tissue engineering benefits of the use of tuneable biomimetic scaffold biomaterials that can be “designed” to act as biocompatible and biomechanically active hybrid interfaces are discussed.

Sign in / Sign up

Export Citation Format

Share Document