scholarly journals Exploiting Response Surface Methodology (RSM) as a Novel Approach for the Optimization of Phenolic and Antioxidant Activity of Date Palm Fruit

2020 ◽  
Vol 14 (4) ◽  
pp. 572-582
Author(s):  
Soumaya Hachani ◽  
◽  
Sarah Boukhalkhal ◽  
Ziyad Ben Ahmed ◽  
Mohamed Harrat ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Palm Fruit ◽  
Ultrasound Extraction ◽  
Solid Ratio ◽  
Methanolic Extracts ◽  
Novel Approach ◽  
Dpph Scavenging

The Box-Behnken design was used to investigate the effect of three independent variables – time, temperature and solvent-to-solid ratio on the responses of total phenolics, total flavonoids, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and cupric ion reducing antioxidant capacity (CUPRAC) of date fruit methanolic extracts. Response surface analysis showed that the optimal ultrasound extraction parameters that maximized the responses were 30 min, 298 K and 74.4 ml/g. Under optimum conditions, UHPLC-DAD-MS/MS was used to tentatively characterize 11 phenolic compounds. The experimental values for the quantification of phenolic compounds and antioxidant activities are in accordance with the predicted values, indicating the suitability of the model and the success of response surface methodology in optimizing the ultrasound extraction conditions.

Optimization of Supercritical Fluid Extraction of Phenolic Compounds from Peach Blossom (Amygdalus Persica) by Response Surface Methodology

2018 ◽  
Vol 17 (2) ◽  
pp. 180-187
Author(s):  
Yang Wen-Bo ◽  
Liu Jie-Chao ◽  
Liu Hui ◽  
Zhang Chun-Ling ◽  
Lv Zhen-Zhen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Fluid Extraction ◽  
Phenolic Contents ◽  
C 30

Supercritical fluid extraction (SFE) was used for the extraction of phenolic compounds from peach blossom (Amygdalus persica). The optimum conditions of SFE were optimized by response surface methodology and confirmed at 64°C, 30 MPa, 143 min, and 35 mL ethanol (100%) as modifier for maximum total phenolic contents (54.10 mg GAE g−1 DW), which was higher than the yield achieved by ultrasonic-assisted extraction (44.04 mg GAE g−1 DW). The antioxidant activities and tyrosinase inhibitory activities of extraction extracted by SFE were 79.55% and 14.42%. Furthermore, chlorogenic acid, quercetin-3-O-galactoside, kaempferol-3-O-galactoside, quercitrin-3-O-rhamnoside, and kaempferol 4′-glucoside were identified in this study.

OPTIMIZATION OF PHYTOESTROGENS EXTRACTION FROM SOY GERM USING RESPONSE SURFACE METHODOLOGY

2021 ◽  
pp. 70-78
Author(s):  
Chau Le Minh
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Extraction Time ◽  
Optimum Conditions ◽  
Potential Health ◽  
Box Behnken Design ◽  
Independent Variables ◽  
Solid Ratio

Phytoestrogens are phytochemicals with antioxidant activities and potential health benefits. Their contents in soy germ is the highest compared to that in parts of soy seed. Response surface methodology (RSM) using Box Behnken Design of four factors was employed to optimize the extraction conditions for phytoestrogens. The Box Behnken Design with five replicates at central point was applied. The four independent variables investigated in this experiment were extraction time (X1) 60-120 minutes, solvent/solid ratio (X2) 8-12, extraction pH (X3) 8-10, ethanol concentration (X4) 50-70%. The high coefficient values indicated that the variables were fitted to the regression for the total phytoestrogens (R2 = 0.9887). Optimum conditions for maximizing total crude phytoestrogen content were 90 minutes for the extraction time, 12/1 for the extraction ratio, 9 for the extraction pH, and 65%  for the ethanol concentration.

scholarly journals Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Soumaya Hasni ◽  
Ghayth Rigane ◽  
Hanene Ghazghazi ◽  
Hajer Riguene ◽  
Amir Bouallegue ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Eucalyptus Marginata ◽  
Esi Ms ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Eucalyptus marginata L. has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. The main goal of the present study was to optimize the extraction parameters of phenolic compounds from Eucalyptus marginata L. leaves using the extraction technique assisted by ultrasound in comparison with maceration using response surface methodology as a predicted tool. Therefore, total phenolic and flavonoid contents have been optimized, taking into account four variables: extraction time, temperature, liquid-to-solid ratio, and ethanol concentration. The optimum ultrasound-assisted extraction method for total phenolic and total flavonoid contents was obtained by ensuring the following parameters: t = 49.9 min, T = 74.9°C, liquid-to-solid ratio = 39.5 ml/g, and ethanol = 58.48%. The optimum extract has been subjected to LC-ESI-MS analysis. This technique allowed us to identify ten phenolic compounds: four phenolic acids mainly gallic acid (27.77 ± 0.06 µg/g DW) and protocatechuic acid (37.66 ± 0.04 µg/g DW) and six flavonoid compounds such as quercetrin (150.78 ± 0.02 µg/g DW) and hyperoside (39.19 ± 0.03 µg/g DW). These green and efficient procedures should be a promising option to guide industrial design for the production of phenolic-rich plant extracts.

scholarly journals Optimization of Ultrasonic Extraction of Phenolic Compounds fromEpimedium brevicornumMaxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Yan Zhao ◽  
Yingying Hou ◽  
Guosheng Tang ◽  
Enbo Cai ◽  
Shuangli Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Extraction Yield ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power

The ultrasound-assisted extraction of phenolic compounds fromEpimedium brevicornuMaxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X1), extraction time (X2), and ratio of aqueous ethanol to raw material (X3), for the achievement of high extraction yield of the phenolic compounds. The optimized conditions areX1of 50% (v/v),X2of 27.5 min, andX3of 250 mL/g. Under these conditions, the experimental yield is 4.29 ± 0.033%(n=3). The antioxidant activity was evaluated using the DPPH assay and ferric-reducing antioxidant power (FRAP). And it indicates that the phenolic compounds fromEpimedium brevicornuMaxim possess significant antioxidant activity. HPLC analysis reveals that the main phenolic compound in the extract product was identified as gallic acid, catechin (Cianidanol), p-hydroxybenzoic acid, vanillic acid, caffeic acid, ferulaic acid, rutin, benzoic acid, and quercetin.

OPTIMIZATION OF EXTRACTION CONDITIONS FOR ANTIOXIDANT PHENOLIC COMPOUNDS FROM MALT ROOTLETS USING RESPONSE SURFACE METHODOLOGY

2009 ◽  
Vol 33 (2) ◽  
pp. 291-305 ◽  
Author(s):  
DE-JING MENG ◽  
JIAN LU ◽  
WEI FAN ◽  
JIAN-JUN DONG ◽  
JIANHUA ZHANG ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface
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