scholarly journals BEBERAPA HASIL INOKULASI PADA CAKRAM DAUN KOPI DENGAN HEMILEIA VASTATRIX DI LABORATORIUM

2002 ◽  
Vol 2 (1) ◽  
pp. 26-31
Author(s):  
Cipta Ginting ◽  
Abdul Gafur ◽  
Rusdi Evizal
Keyword(s):  
Plant Disease ◽  
Disease Incidence ◽  
Disease Development ◽  
Distilled Water ◽  
Hemileia Vastatrix ◽  
Inoculum Sources ◽  
Plant Parts ◽  
Almost All ◽  
Important Disease ◽  
Leaf Disks

Some results of inoculations on coffee leaf disks with Hemileia vastatrix.  Leaf rust caused by Hemileia vastatrix B. et Br. is the most important disease on coffee worldwide.  The obligate nature of H. vastatrix limits research on the disease when inoculation or pathogen growth in laboratory is required.  Inoculation on detached plant parts such as leaf disks may become an alternative to solve the problems.  In this study, the procedure was tested using three coffee varieties (Kartika 1,  USDA, and Robusta) as inoculum sources, leaves of different age, and different uredospore concentrations (4 x 103, 4 x 104, and 4 x 105 uredospores per ml suspension).  The study was done in Laboratory of Plant Disease at University of Lampung from May to December 2001.  Uredospores developed on leaves under field condition were used as inoculum.  The uredospores were taken with sterilized scalpel and placed in sterilized distilled water.  Before doing the experiments, the uredospores were measured using microscope equipped with a micrometer.  The concentration of uredospore suspensions were prepared using hemacytometer.  Leaf disks made with diameter of 2 cm were used in all inoculations.  The results showed that the size of the uredospores varied greatly.  The variation occurred not only among uredospores taken from different lesions  or leaves but also among those taken from the same lesions.  The size of almost all uredospores was the same as that reported in the literature.  Uredospores taken from Kartika (but not from two other varieties) incited disease development and produced symptoms on leaf disks which were made from Kartika coffee.  The age of leaves used to make leaf disks did not influence disease incidence.  Uredospores concentration influenced ( = 0.05) disease incidence on leaf disks with higher concentration caused higher disease incidence .

scholarly journals Survival of Botrytis cinerea as mycelium in rose crop debris and as sclerotia in soil

2005 ◽  
Vol 30 (5) ◽  
pp. 516-521 ◽  
Author(s):  
Alderi E Araújo ◽  
Luiz A Maffia ◽  
Eduardo S. G Mizubuti ◽  
Acelino C Alfenas ◽  
Guy de Capdeville ◽  
...  
Keyword(s):  
Weight Loss ◽  
Botrytis Cinerea ◽  
Rosa Hybrida ◽  
Inoculum Sources ◽  
Disease Epidemiology ◽  
Plant Parts ◽  
Polyethylene Bags ◽  
Stems And Leaves ◽  
Important Disease

Botrytis blight caused by Botrytis cinerea is an important disease of rose (Rosa hybrida) grown in greenhouses in Brazil. As little is known regarding the disease epidemiology under greenhouse conditions, pathogen survival in crop debris and as sclerotia was evaluated. Polyethylene bags with petals, leaves, or stem sections artificially infected with B. cinerea were mixed with crop debris in rose beds, in a commercial plastic greenhouse. High percentage of plant parts with sporulation was detected until 60 days, then sporulation decreased on petals after 120 days, and sharply decreased on stems or leaves after 90 days. Sporulation on petals continued for 360 days, but was not observed on stems after 150 days or leaves after 240 days. Although the fungus survived longer on petals, stems and leaves are also important inoculum sources because high amounts of both are deposited on beds during cultivation. Survival of sclerotia produced on PDA was also quantified. Sclerotia germination was greater than 75% in the initial 210 days and 50% until 360 days. Sclerotia weight gradually declined but they remained viable for 360 days. Sclerotia were produced on the buried petals, mainly after 90 days of burial, but not on leaves or stems. Germination of these sclerotia gradually decreased after 120 days, but lasted until 360 days. Higher weight loss and lower viability were observed on sclerotia produced on petals than on sclerotia produced in vitro

scholarly journals Hops (Humulus lupulus L.) as a Novel Multipurpose Crop for the Mediterranean Region of Europe: Challenges and Opportunities of Their Cultivation

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 484
Author(s):  
Francesco Rossini ◽  
Giuseppe Virga ◽  
Paolo Loreti ◽  
Nicolò Iacuzzi ◽  
Roberto Ruggeri ◽  
...  
Keyword(s):  
Humulus Lupulus ◽  
Specific Reference ◽  
Research Activity ◽  
Plant Parts ◽  
Craft Beer ◽  
Recent Success ◽  
Natural Medicine ◽  
Humulus Lupulus L ◽  
Challenges And Opportunities ◽  
Almost All

The common hop (Humulus lupulus L.) is a dioecious perennial climbing plant, mainly known for the use of its female inflorescences (cones or, simply, “hops”) in the brewing industry. However, the very first interest towards hops was due to its medicinal properties. Actually, the variety of compounds present in almost all plant parts were (and still are) used to treat or prevent several ailments and metabolic disorders, from insomnia to menopausal symptoms as well as obesity and even cancer. Although hops are predominantly grown for hopping beer, the increasing interest in natural medicine is widening new interesting perspectives for this crop. Moreover, the recent success of the craft beer sector all over the world, made the cultivated hop come out from its traditional growing areas. Particularly, in Europe this resulted in a movement towards southern countries such as Italy, which added itself to the already existing hop industry in Portugal and Spain. In these relatively new environments, a complete knowledge and expertise of hop growing practices is lacking. Overall, while many studies were conducted globally on phytochemistry, bioactivity, and the genetics of hops, results from public research activity on basic hop agronomy are very few and discontinuous as well. The objective of this article is to provide an overview of possible uses, phenology, and agronomic aspects of hops, with specific reference to the difficulties and opportunities this crop is experiencing in the new growing areas, under both conventional and organic farming. The present review aims to fill a void still existing for this topic in the literature and to give directions for farmers that want to face the cultivation of such a challenging crop.

scholarly journals Pilonidal sinus disease on the rise: a one-third incidence increase in inpatients in 13 years with substantial regional variation in Germany

2021 ◽  
Author(s):  
Christina Oetzmann von Sochaczewski ◽  
Jan Gödeke
Keyword(s):  
Pilonidal Sinus ◽  
Regional Variation ◽  
Disease Incidence ◽  
Age Groups ◽  
Pilonidal Sinus Disease ◽  
National Guidelines ◽  
Reconstructive Procedures ◽  
Sinus Disease ◽  
The Mean ◽  
Almost All

Abstract Purpose Collective evidence from single-centre studies suggests an increasing incidence of pilonidal sinus disease in the last decades, but population-based data is scarce. Methods We analysed administrative case–based principal diagnoses of pilonidal sinus disease and its surgical therapy between 2005 and 2017 in inpatients. Changes were addressed via linear regression. Results The mean rate of inpatient episodes of pilonidal sinus disease per 100,000 men increased from 43 in 2005 to 56 in 2017. In females, the mean rate of inpatient episodes per 100,000 women rose from 14 in 2005 to 18 in 2017. In the whole population, for every case per 100,000 females, there were 3.1 cases per 100,000 males, but the numbers were highly variable between the age groups. There was considerable regional variation within Germany. Rates of inpatient episodes of pilonidal sinus disease were increasing in almost all age groups and both sexes by almost a third. Surgery was dominated by excision of pilonidal sinus without reconstructive procedures, such as flaps, whose share was around 13% of all procedures, despite recommendations of the national guidelines to prefer flap procedures. Conclusion Rates of inpatient episodes of pilonidal sinus disease in Germany rose across almost all age groups and both sexes with relevant regional variation. The underlying causative factors are unknown. Thus, patient-centred research is necessary to explore them. This should also take cases into account that are solely treated office-based in order to obtain a full-spectrum view of pilonidal sinus disease incidence rates.

scholarly journals Bacterial Leaf Spot of Celery in California: Etiology, Epidemiology, and Role of Contaminated Seed

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 892-896 ◽  
Author(s):  
E. L. Little ◽  
S. T. Koike ◽  
R. L. Gilbertson
Keyword(s):  
Pseudomonas Syringae ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Field Tests ◽  
Hot Water ◽  
Disease Development ◽  
Bacterial Leaf Spot ◽  
Overhead Irrigation ◽  
Celery Seed ◽  
Salinas Valley

Pseudomonas syringae pv. apii, causal agent of bacterial leaf spot (BLS) of celery, was first identified in California in 1989. By 1991, BLS was apparent in all celery-growing areas of the state. Greenhouse-produced transplants were affected most severely, and disease incidence approached 100% in some greenhouses. In this study, sources of inoculum and factors contributing to disease development were investigated in three Salinas Valley greenhouse operations during the 1991, 1992, and 1993 celery transplant seasons (January to August). Epiphytic P. syringae pv. apii was not detected on celery transplants until April or May of each year. Increased epiphytic populations preceded BLS outbreaks, and high-pressure, overhead irrigation favored bacterial infiltration and disease development. In seed-wash assays, P. syringae pv. apii was recovered from 5 of 24 commercial celery seed lots. In field tests, epiphytic P. syringae pv. apii was found on umbels of inoculated celery plants, and seeds from these plants were heavily contaminated with P. syringae pv. apii. Contaminated seed produced seedlings with large epiphytic P. syringae pv. apii populations. Hot-water treatment (50°C for 25 min) eliminated >99.9% of seed contamination. Based on these results, disease management techniques are proposed.

scholarly journals Cultivar and Spacing Effects on Transmission of Phytophthora parasitica in an Ebb-and-Flow Subirrigation System

Plant Disease ◽  
1997 ◽  
Vol 81 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Stephen S. Strong ◽  
Bridget K. Behe ◽  
C. Fred Deneke ◽  
Kira L. Bowen ◽  
Gary J. Keever
Keyword(s):  
Catharanthus Roseus ◽  
Disease Incidence ◽  
Severe Disease ◽  
Disease Development ◽  
Phytophthora Parasitica ◽  
Spacing Effects ◽  
Ebb And Flow

Phytophthora parasitica was transmitted within 6 weeks from vinca (Catharanthus roseus) plants growing in infested potting mix, on the drain end of ebb-and-flow benches, to plants in noninfested potting mix. Transmission of Phytophthora was very low when potting mix was not pasteurized. When potting mix was steam pasteurized, infection of plants, disease incidence, and severity increased with time and decreased with distance from plants in infested pots. The cultivar Pretty in Pink was more susceptible to infection by P. parasitica than cv. Peppermint Cooler, allowing more rapid and severe disease development as well as pathogen dissemination and transmission. Pot spacing did not significantly affect transmission of P. parasitica on an ebb-and-flow bench.

STUDIES ON THE SEEDLING DISEASE OF BARLEY CAUSED BY HELMINTHOSPORIUM SATIVUM P.K. & B.

1956 ◽  
Vol 34 (4) ◽  
pp. 653-673 ◽  
Author(s):  
R. A. Ludwig ◽  
R. V. Clark ◽  
J. B. Julien ◽  
D. B. Robinson
Keyword(s):  
Disease Incidence ◽  
Infested Soil ◽  
Disease Development ◽  
Toxic Activity ◽  
Factors Affecting ◽  
Seedling Disease ◽  
Nutrient Salt ◽  
Helminthosporium Sativum ◽  
Treatment Comparisons

A standard sand – cornmeal – nutrient salt medium, for use in the production of artificial inoculum of Helminthosporium sativum, is described. This inoculum induces uniform plant disease development when thoroughly incorporated with the planting soil. The results presented clearly demonstrate the necessity of using a series of infestation levels in studies of factors affecting disease development in artificially infested soil. It is shown that considerable reliance can be placed on treatment comparisons within an experiment but that comparisons between experiments are much less accurate. The role of a toxin (or toxins) in disease development in barley seedlings has been demonstrated. The toxic activity was found to be distinct from that frequently encountered on addition of organic matter to soil. Results obtained suggest that toxin adsorption by the soil may play an important role in reducing disease incidence and severity.

scholarly journals First Report of Flyspeck Caused by Zygophiala wisconsinensis on Sweet Persimmon Fruit in Korea

Plant Disease ◽  
2011 ◽  
Vol 95 (5) ◽  
pp. 616-616 ◽  
Author(s):  
J. Kim ◽  
O. Choi ◽  
J.-H. Kwon
Keyword(s):  
Disease Incidence ◽  
Morphological Characteristics ◽  
Its Rdna ◽  
Control Treatment ◽  
Diospyros Kaki ◽  
Distilled Water ◽  
First Report ◽  
Persimmon Fruit ◽  
Fungal Isolates ◽  
Agar Disc

Sweet persimmon (Diospyros kaki L.), a fruit tree in the Ebenaceae, is cultivated widely in Korea and Japan, the leading producers worldwide (2). Sweet persimmon fruit with flyspeck symptoms were collected from orchards in the Jinju area of Korea in November 2010. The fruit had fungal clusters of black, round to ovoid, sclerotium-like fungal bodies with no visible evidence of a mycelial mat. Orchard inspections revealed that disease incidence ranged from 10 to 20% in the surveyed area (approximately 10 ha) in 2010. Flyspeck symptoms were observed on immature and mature fruit. Sweet persimmon fruit peels with flyspeck symptoms were removed, dried, and individual speck lesions transferred to potato dextrose agar (PDA) and cultured at 22°C in the dark. Fungal isolates were obtained from flyspeck colonies on 10 sweet persimmon fruit harvested from each of three orchards. Fungal isolates that grew from the lesions were identified based on a previous description (1). To confirm identity of the causal fungus, the complete internal transcribed spacer (ITS) rDNA sequence of a representative isolate was amplified and sequenced using primers ITS1 and ITS4 (4). The resulting 552-bp sequence was deposited in GenBank (Accession No. HQ698923). Comparison with ITS rDNA sequences showed 100% similarity with a sequence of Zygophiala wisconsinensis Batzer & Crous (GenBank Accession No. AY598855), which infects apple. To fulfill Koch's postulates, mature, intact sweet persimmon fruit were surface sterilized with 70% ethanol and dried. Three fungal isolates from this study were grown on PDA for 1 month. A colonized agar disc (5 mm in diameter) of each isolate was cut from the advancing margin of a colony with a sterilized cork borer, transferred to a 1.5-ml Eppendorf tube, and ground into a suspension of mycelial fragments and conidia in a blender with 1 ml of sterile, distilled water. The inoculum of each isolate was applied by swabbing a sweet persimmon fruit with the suspension. Three sweet persimmon fruit were inoculated per isolate. Three fruit were inoculated similarly with sterile, distilled water as the control treatment. After 1 month of incubation in a moist chamber at 22°C, the same fungal fruiting symptoms were reproduced as observed in the orchards, and the fungus was reisolated from these symptoms, but not from the control fruit, which were asymptomatic. On the basis of morphological characteristics of the fungal colonies, ITS sequence, and pathogenicity to persimmon fruit, the fungus was identified as Z. wisconsinensis (1). Flyspeck is readily isolated from sweet persimmon fruit in Korea and other sweet persimmon growing regions (3). The exposure of fruit to unusual weather conditions in Korea in recent years, including drought, and low-temperature and low-light situations in late spring, which are favorable for flyspeck, might be associated with an increase in occurrence of flyspeck on sweet persimmon fruit in Korea. To our knowledge, this is the first report of Z. wisconsinensis causing flyspeck on sweet persimmon in Korea. References: (1) J. C. Batzer et al. Mycologia 100:246, 2008. (2) FAOSTAT Database. Retrieved from http://faostat.fao.org/ , 2008. (3) H. Nasu and H. Kunoh. Plant Dis. 71:361, 1987. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, Inc., New York, 1990.

scholarly journals First report of Colletotrichum siamense Causing Blossom Blight on Thai basil (Ocimum basilicum L.) in Malaysia

Plant Disease ◽  
2020 ◽  
Author(s):  
Siti Izera Ismail ◽  
Nur Adlina Rahim ◽  
Dzarifah Zulperi
Keyword(s):  
Disease Incidence ◽  
Its Region ◽  
Ocimum Basilicum ◽  
Distilled Water ◽  
Single Spore ◽  
First Report ◽  
Plastic Bags ◽  
Colletotrichum Siamense ◽  
Primer Sets ◽  
Acca Sellowiana

Thai basil (Ocimum basilicum L.) is widely cultivated in Malaysia and commonly used for culinary purposes. In March 2019, necrotic lesions were observed on the inflorescences of Thai basil plants with a disease incidence of 60% in Organic Edible Garden Unit, Faculty of Agriculture in the Serdang district (2°59'05.5"N 101°43'59.5"E) of Selangor province, Malaysia. Symptoms appeared as sudden, extensive brown spotting on the inflorescences of Thai basil that coalesced and rapidly expanded to cover the entire inflorescences. Diseased tissues (4×4 mm) were cut from the infected lesions, surface disinfected with 0.5% NaOCl for 1 min, rinsed three times with sterile distilled water, placed onto potato dextrose agar (PDA) plates and incubated at 25°C under 12-h photoperiod for 5 days. A total of 8 single-spore isolates were obtained from all sampled inflorescence tissues. The fungal colonies appeared white, turned grayish black with age and pale yellow on the reverse side. Conidia were one-celled, hyaline, subcylindrical with rounded end and 3 to 4 μm (width) and 13 to 15 μm (length) in size. For fungal identification to species level, genomic DNA of representative isolate (isolate C) was extracted using DNeasy Plant Mini Kit (Qiagen, USA). Internal transcribed spacer (ITS) region, calmodulin (CAL), actin (ACT), and chitin synthase-1 (CHS-1) were amplified using ITS5/ITS4 (White et al. 1990), CL1C/CL2C (Weir et al. 2012), ACT-512F/783R, and CHS-79F/CHS-345R primer sets (Carbone and Kohn 1999), respectively. A BLAST nucleotide search of ITS, CHS-1, CAL and ACT sequences showed 100% similarity to Colletotrichum siamense ex-type cultures strain C1315.2 (GenBank accession nos. ITS: JX010171 and CHS-1: JX009865) and isolate BPDI2 (CAL: FJ917505, ACT: FJ907423). The ITS, CHS-1, CAL and ACT sequences were deposited in GenBank as accession numbers MT571330, MW192791, MW192792 and MW140016. Pathogenicity was confirmed by spraying a spore suspension (1×106 spores/ml) of 7-day-old culture of isolate C onto 10 healthy inflorescences on five healthy Thai basil plants. Ten infloresences from an additional five control plants were only sprayed with sterile distilled water and the inoculated plants were covered with plastic bags for 2 days and maintained in a greenhouse at 28 ± 1°C, 98% relative humidity with a photoperiod of 12-h. Blossom blight symptoms resembling those observed in the field developed after 7 days on all inoculated inflorescences, while inflorescences on control plants remained asymptomatic. The experiment was repeated twice. C. siamense was successfully re-isolated from the infected inflorescences fulfilling Koch’s postulates. C. siamense has been reported causing blossom blight of Uraria in India (Srivastava et al. 2017), anthracnose on dragon fruit in India and fruits of Acca sellowiana in Brazil (Abirami et al. 2019; Fantinel et al. 2017). This pathogen can cause a serious threat to cultivation of Thai basil and there is currently no effective disease management strategy to control this disease. To our knowledge, this is the first report of blossom blight caused by C. siamense on Thai basil in Malaysia.

Sign in / Sign up

Export Citation Format

Share Document