APLIKASI UV SPECTROSCOPY DAN METODE SIMCA UNTUK KLASIFIKASI KOPI LIBERIKA TUNGKAL JAMBI DAN KOPI LIBERIKA PROBOLINGGO

Tungkal Composite Jambi Liberika Coffee is one of the top qualities of Indonesian coffees that has received a geographic indication certificate (IGs). With its limited production and high prices, currently Tungkal Jambi Liberika coffee is one of the coffees that is prone to being counterfeited. The counterfeiting of Tungkal Jambi Liberika coffee is increasingly difficult to identify, especially in the form of ground roasted coffee. This study evaluated the potential application of UV spectroscopy technology to classify Tungkal Jambi Liberika coffee (with geographic indications) and normal Probolinggo Liberika coffee (non- geographic indications). A total of 120 samples for each Liberika coffee were prepared weighing 1 gram for each sample. Spectra measurements were carried out in the form of a coffee solution. Spectral data were taken using a UV-visible spectrometer with a wavelength interval of 200-400 nm (Genesys™ 10S UV-Vis, Thermo Scientific, USA). By using the average transformed spectra in the 250-350 nm interval, the differences between the two types of Liberika coffee can be clearly seen, especially at some wavelength peaks, namely 270 nm, 300 nm, 315 nm and 346 nm. The classification accuracy obtained for the SIMCA classification is 100% for both Tungkal Jambi Liberika and Probolinggo Liberika coffee. Keywords: authentication, classification accuracy, Tungkal Jambi Liberika coffee, SIMCA, UV Spectroscopy

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The Use of UV Spectroscopy and SIMCA for the Authentication of Indonesian Honeys According to Botanical, Entomological and Geographical Origins

Molecules ◽

10.3390/molecules26040915 ◽

2021 ◽

Vol 26 (4) ◽

pp. 915

Author(s):

Diding Suhandy ◽

Meinilwita Yulia

Keyword(s):

Spectral Data ◽

Uv Spectroscopy ◽

Moving Average ◽

Food Product ◽

Classification Method ◽

Economic Losses ◽

Food Fraud ◽

Authentication System ◽

Uv Visible

As a functional food, honey is a food product that is exposed to the risk of food fraud. To mitigate this, the establishment of an authentication system for honey is very important in order to protect both producers and consumers from possible economic losses. This research presents a simple analytical method for the authentication and classification of Indonesian honeys according to their botanical, entomological, and geographical origins using ultraviolet (UV) spectroscopy and SIMCA (soft independent modeling of class analogy). The spectral data of a total of 1040 samples, representing six types of Indonesian honey of different botanical, entomological, and geographical origins, were acquired using a benchtop UV-visible spectrometer (190–400 nm). Three different pre-processing algorithms were simultaneously evaluated; namely an 11-point moving average smoothing, mean normalization, and Savitzky–Golay first derivative with 11 points and second-order polynomial fitting (ordo 2), in order to improve the original spectral data. Chemometrics methods, including exploratory analysis of PCA and SIMCA classification method, was used to classify the honey samples. A clear separation of the six different Indonesian honeys, based on botanical, entomological, and geographical origins, was obtained using PCA calculated from pre-processed spectra from 250–400 nm. The SIMCA classification method provided satisfactory results in classifying honey samples according to their botanical, entomological, and geographical origins and achieved 100% accuracy, sensitivity, and specificity. Several wavelengths were identified (266, 270, 280, 290, 300, 335, and 360 nm) as the most sensitive for discriminating between the different Indonesian honey samples.

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Investigasi pengaruh pengenceran sampel madu pada proses klasifikasi madu menggunakan uv spectroscopy dan kemometrika

Jurnal Teknologi & Industri Hasil Pertanian ◽

10.23960/jtihp.v26i2.72-82 ◽

2021 ◽

Vol 26 (2) ◽

pp. 72

Author(s):

Diding Suhandy ◽

Meinilwita Yulia ◽

Kusumiyati Kusumiyati ◽

Siti Suharyatun ◽

Sri Waluyo

Keyword(s):

Spectral Data ◽

Analytical Methods ◽

Uv Spectroscopy ◽

Honey Sample ◽

Distilled Water ◽

Pca Analysis ◽

Spectra Analysis ◽

Uv Visible ◽

Sample Dilution

One form of honey adulteration is label adulteration for some premium honey such as uniflora honey from the honeybee species Trigona sp. One of the analytical methods that are currently developing and have the potential to perform the classification of premium honey in Indonesia is the UV spectroscopy method. In this study, an investigation was carried out on the effect of dilution on the performance of UV spectroscopy in the process of classifying Indonesian honey with different honeybees. A total of 4 types of honey samples with 10 samples each were used in this study. The honey sample was then diluted using distilled water. Each type of honey was given two dilution treatments, namely 1:20 (volume: volume) dilution of 5 samples and 1:40 (volume: volume) dilution of 5 samples. Spectral data were taken using a UV-visible spectrometer with a wavelength of 190-1100 nm (Genesys™ 10S UV-Vis, Thermo Scientific, USA) using the transmittance mode. The results of spectra analysis generally show that the sample with a 1:20 dilution has a higher absorbance intensity for both the original and modified spectra. The PCA results for each dilution showed that the honey samples could be separated into four different clusters for both 1:20 and 1:40 dilutions. The results of PCA analysis using all samples showed that the honey samples were classified into eight different clusters showing a significant effect of differences in honey sample dilution on the classification process of honey samples based on differences in the types of honeybees.

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VALIDATED RP-HPLC AND UV-SPECTROSCOPY METHODS FOR THE ESTIMATION OF DAPAGLIFLOZIN IN BULK AND IN TABLETS

INDIAN DRUGS ◽

10.53879/id.54.03.10721 ◽

2017 ◽

Vol 54 (03) ◽

pp. 44-51

Author(s):

B. Sabbagh ◽

B. V. S. Lokesh ◽

G. A. Akouwah ◽

Keyword(s):

Good Accuracy ◽

Uv Spectroscopy ◽

Linear Regression Analysis ◽

Molar Absorptivity ◽

Hplc Method ◽

Rp Hplc ◽

Accuracy And Precision ◽

Significant Difference ◽

Uv Visible

Two methods were developed for the determination of dapagliflozin (DAPA) in pure form and in tablets. The procedure utilized was UV-Visible Spectroscopy and RP-HPLC with PDA detector to quantify DAPA in bulk and tablets. The sensitive linear range was identified for both methods within 0.5-5.0μg/mL. The linear regression analysis was identified for both methods with correlation coefficient(r)>0.99. The LOD and LOQ values were found to be 0.05 μg/mL and 0.5 μg/mL for the method by UV-Spectroscopy. The molar absorptivity (ε) was calculated as 1.27 X 105 L.mol-1cm-1. The RP-HPLC method produced LOD and LOQ values of 1.0 ng/mL and 0.5 μg/mL. Both methods were simple, precise, reproducible to quantify the amount of unknown in bulk as well as in tablets and estimated accurately within the range of 100.0±0.5%. Statistical analysis was performed on the data obtained. There was no significant difference between the developed and reported methods with p>0.05. Both methods can be applied for routine analysis of DAPA in bulk and tablets with good accuracy and precision.

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Some complexes of N-aryl furfural nitrones with Co (II), Ni (II), Cu (II), Zn (II) and Cd (II) chlorides

International Journal of Advanced Chemistry ◽

10.14419/ijac.v4i1.6086 ◽

2016 ◽

Vol 4 (1) ◽

pp. 10

Author(s):

Amer A. Taqa

Keyword(s):

Spectral Data ◽

General Formula ◽

Elemental Analysis ◽

13C Nmr ◽

High Frequency ◽

Metal Ion ◽

Uv Spectroscopy ◽

Active Atom ◽

Tetrahedral Complexes

Some new metal(II) dichloride complexes with the ligands substituted nitrones of the general formula [ML2Cl2], where M= Co(II), Ni(II), Cu(II), Zn(II) and Cd(II), L=OCH=CHCH=C-CH=N(O)C6H4X (X=H,p-CH3,CH3O,CH3CO,F,Cl,and Br) have been prepared and characterized by elemental analysis, IR,1H,13C NMR and Vis/Uv spectroscopy. The IR spectral data showed that the nitrone ligands coordinated with the metal ion through the most active atom of the N-oxide to give square planner coordinate (Cu,Ni,) complexes and (Zn,Cd,Co) tetrahedral complexes. No correlation was observed between the N-O vibrations stretching high frequency ν (N-O) of the complexes and the Hammet (σ) constants.

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