scholarly journals Pengaruh Pemberian Whey Protein Terhadap Pengurangan Gejala Kerusakan Otot Setelah Aktivitas Eksentrik

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Reno Siska Sari

Aktivitas eksentrik adalah salah satu jenis aktivitas resistance yang sering menimbulkan kerusakan otot, yang dimulai dari 24 jam setelah aktivitas dan mencapai puncaknya pada 48 jam setelah aktivitas. Kerusakan otot menyebabkan hilangnya kekuatan otot, menurunkan range of motion sendi, meningkatkan inflamasi dan konsentrasi protein miofibril dalam darah. Rangsangan sintesis protein dan meminimalisasi muscle protein breakdown (proteolisis) adalah dua proses seluler yang penting untuk pemulihan setelah kerusakan otot. Tujuan dari penelitian ini adalah untuk membuktikan pengaruh suplementasi whey protein terhadap pengurangan gejala kerusakan otot setelah aktivitas eksentrik. Subjek penelitian ini adalah mahasiswa PJKR IKIP Budi Utomo yang dibagi secara acak ke dalam dua kelompok, 22 orang coba kelompok kontrol dan 22 orang coba kelompok perlakuan. Desain penelitian ini adalah randomized group pretest and posttest design. Aktivitas eksentrik yang dilakukan adalah aktivitas Drop Jumps pada bangku dengan ketinggian 0.5 meter. Whey protein diberikan setelah aktivitas eksentrik sebanyak 20 gram/subjek. Dalam penelitian ini whey protein diberikan dalam bentuk serbuk yang dilarutkan dengan 240 ml air mineral. Waktu pemberian whey protein dilakukan segera setelah aktivitas eksentrik, 24 dan 48 jam setelah aktivitas eksentrik (selama 3 hari pemulihan). Pengukuran kekuatan otot tungkai menggunakan back and leg dynamometer sedangkan pengukuran ROM sendi menggunakan alat goniometer. Dari hasil penelitian diketahui bahwa pemberian asupan whey protein pada kelompok perlakuan setelah aktivitas eksentrik dapat meningkatkan kekuatan otot tungkai pada 48 jam (72,3±16,1kg) dengan nilai p=0,003 (p<0,05) dan pada 72 jam (80,3±17,2) dengan nilai p=0,00 (p<0,05). Pemberian asupan whey protein pada kelompok perlakuan setelah aktivitas eksentrik juga dapat meningkatkan ROM sendi lutut pada 72 jam (130,8±3,1) whey protein sebesar 20 gram /hari setelah aktivitas eksentrik dapat meningkatkan kekuatan otot tungkai lutut pada jam ke 48 dan 72 serta ROM sendi pada jam ke 72

1998 ◽  
Vol 275 (6) ◽  
pp. R1983-R1991 ◽  
Author(s):  
Arthur Williams ◽  
Jing Jing Wang ◽  
Li Wang ◽  
Xiaoyan Sun ◽  
Josef E. Fischer ◽  
...  

We tested the role of interleukin-6 (IL-6) in sepsis-induced muscle proteolysis by determining ubiquitin mRNA levels and protein breakdown rates in incubated extensor digitorum longus muscles from septic and sham-operated IL-6 knockout and wild-type mice. In addition, the effect of treatment of mice with human recombinant IL-6 on muscle protein breakdown rates was determined. Finally, protein breakdown rates were measured in myotubes treated for up to 48 h with different concentrations of IL-6. Sepsis in wild-type mice resulted in an approximately ninefold increase in plasma IL-6 levels, whereas IL-6 was not detectable in plasma of sham-operated or septic IL-6 knockout mice. Total and myofibrillar muscle protein breakdown rates were increased by ∼30% and threefold, respectively, in septic IL-6 wild-type mice with an almost identical response noted in septic IL-6 knockout mice. Ubiquitin mRNA levels determined by dot blot analysis were increased during sepsis in muscles from both IL-6 knockout and wild-type mice, although the increase was less pronounced in IL-6 knockout than in wild-type mice. Treatment of normal mice or of cultured L6 myotubes with IL-6 did not influence protein breakdown rates. The present results suggest that IL-6 does not regulate muscle proteolysis during sepsis.


Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1177
Author(s):  
Wataru Kume ◽  
Jun Yasuda ◽  
Takeshi Hashimoto

Background: Combining resistance exercise (RE) with nutrient intake stimulates muscle protein net balance. However, it is still unclear whether the optimal timing of nutrient intake is before or after RE, especially on muscle protein breakdown (MPB) for an augmented muscle anabolic response. The aim of this study was to investigate the effect of a substantial mixed meal (i.e., nutrient- and protein-dense whole foods) before or after RE, compared with RE without a meal on the acute response of MPB in a crossover-design study. Methods: Eight healthy young men performed three trials: (1) meal intake before RE (Pre), (2) meal intake after RE (Post), and (3) RE without meal intake (No). Plasma insulin and 3-methylhistidine (3-MH), an MPB marker, were measured. Results: Time course change in plasma insulin level after RE was significantly higher in the Post condition than in the Pre and No conditions. The area under the curve of 3-MH concentration was significantly lower in the Post condition than in the Pre and No conditions. Conclusions: These results suggest that a substantial mixed meal immediately after RE may effectively suppress MPB in the morning.


2018 ◽  
Vol 48 (S1) ◽  
pp. 53-64 ◽  
Author(s):  
Kevin D. Tipton ◽  
D. Lee Hamilton ◽  
Iain J. Gallagher

2010 ◽  
Vol 299 (2) ◽  
pp. R533-R540 ◽  
Author(s):  
Erin L. Glynn ◽  
Christopher S. Fry ◽  
Micah J. Drummond ◽  
Hans C. Dreyer ◽  
Shaheen Dhanani ◽  
...  

Muscle protein breakdown (MPB) is increased following resistance exercise, but ingestion of carbohydrate during postexercise recovery can decrease MPB with no effect on muscle protein synthesis (MPS). We sought to determine whether a combination of essential amino acids (EAA) with low carbohydrate or high carbohydrate could effectively reduce MPB following resistance exercise and improve muscle protein net balance (NB). We hypothesized that higher levels of carbohydrate and resulting increases in circulating insulin would inhibit MPB and associated signaling, resulting in augmented NB. Thirteen male subjects were assigned to one of two groups receiving equivalent amounts of EAA (∼20 g) but differing carbohydrate levels (low = 30, high = 90 g). Groups ingested nutrients 1 h after an acute bout of leg resistance exercise. Leg phenylalanine kinetics (e.g., MPB, MPS, NB), signaling proteins, and mRNA expression were assessed on successive muscle biopsies using stable isotopic techniques, immunoblotting, and real-time quantitative PCR, respectively. MPB tended to decrease ( P < 0.1) and MPS increased ( P < 0.05) similarly in both groups following nutrient ingestion. No group differences were observed, but muscle ring finger 1 (MuRF1) protein content and MuRF1 mRNA expression increased following resistance exercise and remained elevated following nutrient ingestion, while autophagy marker (light-chain 3B-II) decreased after nutrient ingestion ( P < 0.05). Forkhead box-O3a phosphorylation, total muscle atrophy F-box (MAFbx) protein, and MAFbx and caspase-3 mRNA expression were unchanged. We conclude that the enhanced muscle protein anabolic response detected when EAA+carbohydrate are ingested postresistance exercise is primarily due to an increase in MPS with minor changes in MPB, regardless of carbohydrate dose or circulating insulin level.


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