scholarly journals MORPHOLOGICAL CHARACTERISTICS OF THE ANTERIOR ABDOMINAL WALL TIS-SUES IN CASES OF IMPLANTATION OF POLYPROPYLENE MESH WITH PRF MEMBRANE

2018 ◽  
Vol 17 (3) ◽  
pp. 16-23
Author(s):  
V. I. Pyatnochka ◽  
I. Ya. Dzyubanovsʹkyy ◽  
K. S. Volkov
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Plasma Membranes ◽  
Anterior Abdominal Wall ◽  
Morphological Characteristics ◽  
Experimental Period ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Mesh Material ◽  
Favourable Environment

According to the experimental study on Vietnamese pigs after implantation of a polypropylene mesh with PRF membrane into retromuscular space, the results of ultrastructural changes in the cells of muscular aponeurotic layer of abdominal wall were presented. It was proved that on the 14th day of the experiment, inflammatory changes in tissues were expressed significantly less than in cases of the isolated implantation of the polypropylene mesh without PRF membrane. Activation of fibroblasts and signs of fiber structures development around the mesh material increased. In the late experimental period (28 days), at the microscopic and electron-microscopic levels the minor manifestations of inflammatory reaction, improved microcirculation in the area of implantation of the mesh with PRF membrane were found that in turn contributed to the increased activity of fibroblasts and development of collagen fibers around the mesh material. The use of PRF plasma membranes stimulated an active development of new capillaries, improved blood flow, accelerated metabolic processes in tissues, and suddenly increased development of collagen, hyaluronic acid that in turn created favourable environment for a complete integration of the polypropylene mesh into the muscular aponeurotic layer of anterior abdominal wall tissue.

scholarly journals COMPARATIVE ULTRASTRUCTURAL CHARACTERISTICS OF THE EFFECT OF “LIGHTWEIGHT” AND “HEAVYWEIGHT” POLYPROPYLENE MESHES ON THE PRO-CESSES OF ANTERIOR ABDOMINAL WALL TISSUE REGENERATION WHEN PERFORMING RETROMUSCULAR ALLOGENEOPLASTY

2018 ◽  
Vol 17 (2) ◽  
pp. 76-83
Author(s):  
V. I. Pyatnochk ◽  
I. Ya. Dzyubanovsʹkyy ◽  
K. S. Volkov
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Anterior Abdominal Wall ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Ultrastructural Changes ◽  
Lightweight Mesh ◽  
Aseptic Inflammation ◽  
Ultrastructural Studies ◽  
Tissue Reactions

The ultrastructural changes of the tissues of musculo-aponeurotic layer of anterior abdominal wall after implantation of the “lightweight” and “heavyweight” polypropylene meshes into the retrometallic space were studied in the experiment. Significant differences of the tissue reactions at different periods of the experiment on the implanted material were evidenced. The presence of a moderately pronounced inflammatory tissue reaction was noticed on the 14th day of the study in the area of the implantation of the “lightweight” polypropylene mesh compared to the “heavyweight” one: the inflammatory response included acute manifestations of aseptic inflammation with a significant tissue response. On the 28th day of the experiment, in cases of implantation of the “lightweight” mesh, the stabilization of tissue reaction to the implant with reduction of inflammatory changes was observed, as evidenced by a significantly smaller number of leukocyte infiltration sites, a significant improvement in vascularization and development of collagen fibres around the mesh material with fibroblasts, which was not observed in the area of implantation of the “heavyweight” polypropylene mesh. The conducted morphological and ultrastructural studies have prioritized the use of “lightweight” meshes when choosing material to perform allohernioplasty.

Two action sites of AM-toxin I produced by apple pathotype of Alternaria alternata in host cells: an ultrastructural study

1981 ◽  
Vol 59 (3) ◽  
pp. 301-310 ◽  
Author(s):  
Pyoyun Park ◽  
Syoyo Nishimura ◽  
Keisuke Kohmoto ◽  
Hiroshi Otani ◽  
Kazuyuki Tsujimoto
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Amorphous Materials ◽  
Plasma Membranes ◽  
Host Cells ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Electron Microscopic Autoradiography ◽  
Moderately Resistant ◽  
Membrane Cell

The localization of primary action sites of AM-toxin I in host cells was examined by ultrastructural investigation and electron microscopic autoradiography. In susceptible apple leaves, the first effect of the toxin appeared 1 h after treatment in the plasma membranes and chloroplasts of mesophyll and vascular bundle sheath cells and in the plasma membranes of phloem and epidermal cells. Membranes and vesicles which were stained positively with a specific staining solution for grana lamellae were found in the matrix of the chloroplasts, showing that the membranous materials were derived from the disrupted grana. Cell wall lesions were formed around plasmodesmata where plasma membranes were invaginated. The invaginated sites were filled with amorphous materials from degraded cell walls, including membranes derived from plasma membranes and the desmotubules extending from plasmodesmata. The modified chloroplasts and plasma membranes were observed more often as the time after the toxin treatment was prolonged. Modified plastids were not found in the leaf cells. The other cellular membranes appeared normal even 10 h after the treatment. Resistant leaf cells were rarely affected by the toxin. Not all tissues from susceptible apples were sensitive as the toxin caused no necrosis or ultrastructural changes in petal cells. Resistant petal cells were also insensitive to the toxin, but the toxin causes necrosis and ultrastructural changes in moderately resistant petal cells in which the primary effect of the toxin appeared as plasma membrane modifications. Plastids were not affected by the toxin. These results indicate that the action sites of the toxin may be located on the plasma membrane – cell wall association in susceptible leaf cells and in moderately resistant petal cells and also on the chloroplasts of susceptible cells. The results of electron microscopic autoradiography also provided evidence that the action sites of the toxin were present on chloroplasts and the plasma membrane –cell wall association of susceptible leaf cells.

scholarly journals APPLICATION OF MODIFIED NICKELIDE TITANIUM IMPLANTS TO REPLACE ABDOMINAL WII DEFECTS IN EXPERIMENT

2019 ◽  
Vol 21 (1) ◽  
pp. 129-132
Author(s):  
T V Cherepanova
Keyword(s):  
Abdominal Wall ◽  
Titanium Nickelide ◽  
Anterior Abdominal Wall ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Nickel Titanium ◽  
Adhesive Properties ◽  
Mesh Material ◽  
Mesh Implant ◽  
High Degree

An important step towards the development of reticular endorotesis was the introduction and use of modern super-elastic material based on nickel-titanium. The article is devoted to a new way to solve one of the urgent problems of surgery. The experiment proved the possibility of an intraperitoneal method of disposing of a nickel-mesh titanium implant based on titanium nickelide modified by auto-coupling tissue in artificially created defects of the anterior abdominal wall, and anti-adhesive properties of the combined mesh material are shown. In the experiment, performed a series of experiments on 33 rabbits of the Chinchilla breed. Animals were divided into 3 groups of 11 animals each. The experiment included 2 stages. In the first part, netting of nickelide titanium implant was performed in the posterior pelvic limb of the animal. The second stage was carried out by replacing the artificially created defect of the anterior abdominal wall with a mesh implant based on titanium nickelide modified with connective tissue. The experiment proved the possibility of an intraperitoneal method of disposing of a nickel-mesh titanium implant based on titanium nickelide modified by auto-coupling tissue in artificially created defects of the anterior abdominal wall, and anti-adhesive properties of the combined mesh material are shown. The use of super-elastic reticular nickel-titanium implants in combination with an auto-connecting tissue will, with a high degree of effectiveness, replace the lost structures of the anterior abdominal wall in patients with postoperative ventral hernias.

scholarly journals Aloplasty of oncisional ventral hernias of using nanomodified polypropilene mesh

2019 ◽  
Keyword(s):  
Abdominal Wall ◽  
Operative Treatment ◽  
Polypropylene Mesh ◽  
Anterior Abdominal Wall ◽  
Long Term Results ◽  
Postoperative Wound ◽  
Group I ◽  
Method Of Placement ◽  
Group Ii ◽  
Ventral Hernias

Aloplasty of incisional ventral hernia (IVH), method of placement and fixation nanomodified polypropylene mesh retro muscular, buth this is accompanied by a fairly high freguency of postoperative complications from the postoperative wound. In our view, the use of a nanomodified polypropylene mesh modified by carbon nanotubes and an antiseptic of polyhexamethlene guanidme chloride in combination with the method of placement and fixation retro muscular the results of operative treatment of IVH. Aim – to improve the results of operative treatment of incisional ventral hernias in combination with the method of placement and fixation retro muscular nanomodified polypropylene mesh. Materials and methods. The analysis of operative treatment of 148 patients with IVH of has been performed. Depending on the type of mesh used during surgical treatment, patients were divided into 2 groups. In 74 (50%) of Group I patients, method of placement and fixation nanomodified polypropylene mesh retro muscular. In the 2nd group, 74 (50%) patients method of placement and fixation retro muscular of a classic polypropylene mesh. Results and discussion. Statistically significant results were obtained in patients of Group I compared to Group II: seroma was in 24 (32.4±1.2%) in Group II compared to 5 (6.8±0.5%) in Group I (p<0.05), respectively, the suppuration of the postoperative wound – 7 (9.5±0.5%) to 1 (1.4±0.2%) (p<0.05). The terms of stay of patients of group II on inpatient treatment – 12,1±2,3 days group II – 7,1±1,1 days. Long-term results: ligature fistulas of the anterior abdominal wall were detected in 5 (7.7±0.5%) patients of group II, in patients of group I of the ligature fistulas were not detected (p<0.05), meshoma – in 3 (4.6±0.3%) of patients in group II, in group I there was no stir (p>0.05). Chronic pain in the abdominal wall in 6 – 8 months after surgery was observed in 5 (7.7±0.6)% patients in group II and in 1 (1.5±0.2%) group I (p>0.05), recurrences of hernia were found in 6 (9.2±0.6%) patients of group II, in group I – in 1 (1.5±0.2)% (p<0.05). Conclusion. Operative treatment of IVH method of placement and fixation nanomodified polypropylene mesh retro muscular is more effective compared with the use of the classical polypropylene mesh, namely, reducing the freguency of seroma from 32.4±1.2% in the II group of patients to 6.8±0.5% in group I, respectively, suppurations of postoperative wounds – from 9.5±0.5% to 1.4±0.2%, inflammatory infiltrates – from 12.2±0.6% to 1.4±0.2%, ligaturial fistulas of the anterior abdominal wall – from 7.7±0.5% to 0%, meshoma – from 4.6±0.3% to 0%, chronic postoperative pain – from 7.7±0.6% to 1.5±0.2%, recurrence of hernia–from 9.2±0.6% to 1.5±0.2%.

An electron microscopic study of the early changes proximal to a constriction in sympathetic nerves

1969 ◽  
Vol 172 (1026) ◽  
pp. 39-51 ◽  
Keyword(s):  
Plasma Membranes ◽  
Sympathetic Nerves ◽  
Ultrastructural Changes ◽  
Mechanical Trauma ◽  
Tubular Structures ◽  
Electron Microscopic ◽  
Axonal Swelling ◽  
Electron Dense Core ◽  
Myelin Figures

The ultrastructural changes, proximal to a constriction, in unmyelinated postganglionic sympathetic axons have been studied in the cat splenic and hypogastric nerves. They were con stricted with a fine ligature, which was left in situ , and examined at intervals up to 24h after operation. Immediately after tying the ligature there were definite changes in the morphology and organelle content of the axons adjacent to the constriction. These were due to the mechanical trauma produced by tying the ligature. From 10 min onwards there was a rapid accumulation of organelles in axons, which became progressively more swollen. Eventually the plasma membranes of both axons and Schwann cells broke down and later the Sclrwann cell basement membrane ruptured , allowing axonal organelles to escape into the interstitial spaces. The accumulating organelles included vesicles with an electron dense core, mitochondria, large vacuoles and myelin figures. Filamentous structures and both fine and wide tubules were more prominent and more numerous after operation. These were accompanied by a marked increase in the number of agranular vesicles. Tubular structures, morphologically similar to both the agranular vesicles and the vacuoles, were also very numerous. This similarity in appearance and the fact that many of these agranular vesicles, vacuoles and tubules were found joined together by either filaments or fine tubules suggests that they are part of the same system . At all times the greatest accumulation of organelles and the most marked axonal swelling was found in the first 0.5 to 1 mm segment of nerve immediately adjacent to the constriction. Less marked changes, affecting principally the agranular vesicles and finer tubules to gether with focal accumulations of mitochondria were seen at more proximal levels from 4h onwards. It is suggested that the axonal swelling is due to the accumulation of axoplasm and organelles. Two possible interpretations are discussed: (1) that this represents the effects of obstructing the normal flow of axoplasm from the cell body to the periphery, and (2) that some features, in particular the increase in the size and number of the various tubules may be indicative of an active reaction to the effects of axonal injury. These two phenomena probably take place simultaneously and are accompanied by degenerative changes.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

Morphologic alteration in the muscles of patients with hypokalemic periodic paralysis or myopathy

1990 ◽  
Vol 48 (3) ◽  
pp. 222-223
Author(s):  
T. Shimizu ◽  
Y. Muranaka ◽  
I. Ohta ◽  
N. Honda
Keyword(s):  
Clinical Manifestations ◽  
Quadriceps Muscle ◽  
Honeycomb Structure ◽  
Periodic Paralysis ◽  
Ultrastructural Changes ◽  
Hypokalemic Periodic Paralysis ◽  
Electron Microscopic ◽  
Tubular Aggregates ◽  
Hypokalemic Myopathy ◽  
Transmission Electron

There have been many reports on ultrastructural alterations in muscles of hypokalemic periodic paralysis (hpp) and hypokalemic myopathy(hm). It is stressed in those reports that tubular structures such as tubular aggregates are usually to be found in hpp as a characteristic feature, but not in hm. We analyzed the histological differences between hpp and hm, comparing their clinical manifestations and morphologic changes in muscles. Materials analyzed were biopsied muscles from 18 patients which showed muscular symptoms due to hypokalemia. The muscle specimens were obtained by means of biopsy from quadriceps muscle and fixed with 2% glutaraldehyde (pH 7.4) and analyzed by ordinary method and modified Golgimethod. The ultrathin section were examined in JEOL 200CX transmission electron microscopy.Electron microscopic examinations disclosed dilated t-system and terminal cistern of sarcoplasmic reticulum (SR)(Fig 1), and an unique structure like “sixad” was occasionally observed in some specimens (Fig 2). Tubular aggregates (Fig 3) and honeycomb structure (Fig 4) were also common characteristic structures in all cases. These ultrastructural changes were common in both the hypokalemic periodic paralysis and the hypokalemic myopathy, regardless of the time of biopsy or the duration of hypokalemia suffered.

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