scholarly journals Synchrotron IR-microspectroscopy-based visualization of molecular and chemical interactions between dental cement, biomimetic composite and native dental tissue

2019 ◽  
pp. 71-78 ◽  
Author(s):  
DL Goloshchapov ◽  
V.M. Kashkarov ◽  
Yu.A. Ippolitov ◽  
I.Yu. Ippolitov ◽  
Jitraporn Vongsvivut ◽  
...  
Keyword(s):  
Calcium Hydroxyapatite ◽  
Human Tooth ◽  
Hard Tissue ◽  
Dental Tissue ◽  
Human Teeth ◽  
Dental Cement ◽  
Biomimetic Material ◽  
Hard Dental Tissue ◽  
Calcium Source ◽  
Ir Microspectroscopy

The low affinity of composite materials for the hard tissue of human teeth poses a challenge to restorative dentists. This study was undertaken to explore molecular and chemical characteristics of the interface between the dental cement, the buffer layer formed from a next generation biomimetic material that mimics the organic mineral composition of human enamel and dentin, and the intact native hard dental tissue. Seven plane-parallel dental slices were analyzed using synchrotron IR microspectroscopy. The obtained absorption spectra of functional molecular groups were organized into cluster maps. This allowed us to identify the intact tissue, the adhesive agent and the biomimetic layer at their interface and to localize and measure concentrations of functional groups involved in the integration of the biomimetic composite into the hard tissue of the human tooth. The proposed biomimetic material is based on nanocrystal carbonate-substituted calcium hydroxyapatite synthesized from a biogenic calcium source and a complex of basic polar amino acids copying the composition of the human tooth and can form a functional bond with hard dental tissue.

Surface Micromorphology and Crystalline Structures of Hard Dental Tissue Treated by Atmospheric Pressure Plasma Jet

2019 ◽  
Author(s):  
Negin Beryani Nezafat ◽  
Slawomir Kulesza ◽  
Mahmood Ghoranneviss ◽  
Seyed Mohammad Elahi ◽  
Miroslaw Bramowicz ◽  
...  
Keyword(s):  
Rietveld Method ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
X Ray Diffraction ◽  
Dental Tissue ◽  
Human Teeth ◽  
Spectra Analysis ◽  
X Ray ◽  
Surface Micromorphology ◽  
Hard Dental Tissue

In this paper, the results of helium plasma jet treatment of hard tissues of natural human teeth including enamel, dentin, and cementum are presented. Changes in surface morphology and structural properties have been investigated using scanning electron microscopy (SEM) in connection with multiscale image analysis, X-ray diffraction (XRD) with the aid of the Rietveld method and Williamson-Hall plots, energy dispersive X-ray (EDX) and Raman spectra analysis. Despite initial differences in scaling behaviors of the surfaces of freshly extracted tissues, spatial characteristics of their plasma-treated counterparts appeared similar taking into account the fractal measures. On the other hand, despite improvement in crystalline structure of enamel due to the plasma treatments, the structure of cementum has been found intact.

scholarly journals Biomimetic nano-c-HAp hybrid layer engineering and determination of mechanisms of its integration with native hard dental tissue

2021 ◽  
pp. 100266
Author(s):  
Pavel Seredin ◽  
Dmitry Goloshchapov ◽  
Vladimir Kashkarov ◽  
YuYu Khudyakov ◽  
Ivan Ippolitov ◽  
...  
Keyword(s):  
Hybrid Layer ◽  
Dental Tissue ◽  
Hard Dental Tissue

scholarly journals The mineral content of the hard dental tissue of mesiodens

2018 ◽  
Vol 162 (2) ◽  
pp. 149-153 ◽  
Author(s):  
Sanja Tanaskovic-Stankovic ◽  
Irena Tanaskovic ◽  
Nemanja Jovicic ◽  
Marina Miletic-Kovacevic ◽  
Tatjana Kanjevac ◽  
...  
Keyword(s):  
Mineral Content ◽  
Dental Tissue ◽  
Hard Dental Tissue

scholarly journals Development of A Three-Dimensional Tissue Construct from Dental Human Ectomesenchymal Stem Cells: In Vitro and In Vivo Study

2012 ◽  
Vol 6 (1) ◽  
pp. 226-234 ◽  
Author(s):  
Daniela Guzmán-Uribe ◽  
Keila Neri Alvarado Estrada ◽  
Amaury de Jesús Pozos Guillén ◽  
Silvia Martín Pérez ◽  
Raúl Rosales Ibáñez
Keyword(s):  
Three Dimensional ◽  
Human Tooth ◽  
Animal Cells ◽  
Dental Tissue ◽  
Membrane Markers ◽  
Tissue Organization ◽  
Specific Membrane ◽  
Tissue Construct

Application of regenerative medicine technology provides treatment for patients with several clinical problems, like loss of tissue and its function. The investigation of biological tooth replacement, dental tissue engineering and cell culture, scaffolds and growth factors are considered essential. Currently, studies reported on the making of threedimensional tissue constructs focused on the use of animal cells in the early stages of embryogenesis applied to young biomodels. The purpose of this study was the development and characterization of a three-dimensional tissue construct from human dental cells. The construct was detached, cultured and characterized in mesenchymal and epithelial cells of a human tooth germ of a 12 year old patient. The cells were characterized by specific membrane markers (STRO1, CD44), making a biocomplex using Pura Matrix as a scaffold, and it was incubated for four days and transplanted into 30 adult immunosuppressed male Wistar rats. They were evaluated at 6 days, 10 days and 2 months, obtaining histological sections stained with hematoxylin and eosin. Cell cultures were positive for specific membrane markers, showing evident deviations in morphology under phase contrast microscope. Differentiation and organization were noted at 10 days, while the constructs at 2 months showed a clear difference in morphology, organization and cell type. It was possible to obtain a three-dimensional tissue construct from human dental ectomesenchymal cells achieving a degree of tissue organization that corresponds to the presence of cellular stratification and extracellular matrix.

scholarly journals Microleakage of glass ionomer cement restorations

2009 ◽  
Vol 56 (2) ◽  
pp. 78-85 ◽  
Author(s):  
Lado Davidovic ◽  
Slavoljub Tomic ◽  
Mihael Stanojevic ◽  
Slavoljub Zivkovic
Keyword(s):  
Glass Ionomer Cement ◽  
Sem Analysis ◽  
Glass Ionomer ◽  
Penetration Test ◽  
Dental Tissue ◽  
Class V ◽  
Oral Side ◽  
Dye Penetration ◽  
Hard Dental Tissue ◽  
Ionomer Cement

Introduction. A lack of appropriate adhesiveness is one of the biggest problems in the restorative dentistry today and the main cause of the microleakage between restorations and hard dental tissue. The aim of this study was to assess the adhesiveness of two different glass ionomer cement restorations class V on the hard dental tissue using the SEM analysis and dye penetration test. Material and methods. The study included 80 extracted teeth for orthodontic reasons (premolars and molars) in both genders and different age. On the vestibular and oral side of the teeth, adhesive preparations class V were done (size 3?2?2 mm). On the vestibular preparation, GC Fuji II was applicated and GC Fuji II LC-improved on the oral side. The quality of the adhesiveness between restorations and hard dental tissue was evaluated using the SEM analysis and dye penetration test (0.5% basic fuxsin). Linear penetration of the dye was observed using 10 times magnification. Results. The results showed that microleakage was presented with both materials but a ratio was less with Fuji II LC compared with Fuji II. Also, the microleakage was less on the occlusal parts of the restorations than on the gingival, with both materials. Microleakage was noticed in 93.44% teeth with Fuji II GJC restorations with index of microleakage of 148 on all edges. Fuji II LC GJC restorations showed microleakage in 68.4% teeth with index of microleakage 75 on all edges. The SEM analysis showed that both glass ionomer materials had better adhesiveness to the enamel than to the dentin. The average gap length between Fuji II LC and dentin was 9 ?m and Fuji II 17 ?m, respectively. Conclusion. Better adhesiveness to the hard dental tissue was achieved with materials of the newer generations, resin modificated glas ionomer cements.

Structural and microstructural characterization of bioapatites and synthetic hydroxyapatite using X-ray powder diffraction and Fourier transform infrared techniques

2006 ◽  
Vol 39 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Santu Chakraborty ◽  
Sandip Bag ◽  
Subrata Pal ◽  
Alok K. Mukherjee
Keyword(s):  
Fourier Transform ◽  
Powder Diffraction ◽  
Microstructural Characterization ◽  
Fourier Transform Infrared ◽  
Calcium Hydroxyapatite ◽  
Partial Replacement ◽  
Human Teeth ◽  
X Ray ◽  
Rabbit Bone ◽  
Synthetic Hydroxyapatite

Bioapatite, the main constituent of mineralized tissue in mammalian bones and teeth, is similar in structure and composition to calcium hydroxyapatite (HAP), Ca10(PO4)6(OH)2. The crystallographic analyses of four bioapatites of human-, goat- and rabbit-bone and human-teeth origin, and one synthetic HAP preparedviamicrowave irradiation, have been carried out using X-ray powder diffraction techniques. Fourier transform infrared spectra and Rietveld analyses indicate partial replacement of PO43−ions by CO32−ions in all bioapatites.

Advances in Ablation Optics of Hard Dental Tissue

2012 ◽  
Vol 49 (10) ◽  
pp. 100005
Author(s):  
詹振林 Zhan Zhenlin ◽  
张先增 Zhang Xianzeng ◽  
郭文庆 Guo Wenqing ◽  
刘海山 Liu Haishan ◽  
谢树森 Xie Shusen
Keyword(s):  
Dental Tissue ◽  
Hard Dental Tissue

scholarly journals The Effect of Desensitizing Toothpastes and Coffee Staining on the Optical Properties of Natural Teeth and Microhybrid Resin Composites: An In-Vitro Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Sedanur Turgut ◽  
Hamiyet Kılınç ◽  
Kıvanç Utku Ulusoy ◽  
Bora Bagis
Keyword(s):  
Color Change ◽  
Cetylpyridinium Chloride ◽  
Human Tooth ◽  
Staining Procedure ◽  
Control Group ◽  
Human Teeth ◽  
Color Changes ◽  
Stannous Fluoride ◽  
Color Measurements ◽  
Natural Teeth

Objective. To evaluate the effect of different desensitizing toothpastes and coffee staining on the discoloration rate of natural human tooth and composite materials. Materials and Methods. A total of 56 human teeth and 56 composite specimens were used. After initial color measurements were taken, specimens and teeth were exposed to simulated toothbrushing with six desensitizing toothpastes containing different active ingredients: stannous fluoride, strontium acetate, potassium citrate and hydroxyapatite, cetylpyridinium chloride, arginine, and novamin. Specimens were then exposed to coffee staining. Throughout the staining procedure, the storing solution was refreshed every day and the specimens were brushed with the tested toothpastes. Color measurements and changes were recorded at each stage and analyzed with one-way ANOVA, Dunnett tests, and paired sample t-test (p<0.05). Results. The largest color change was obtained with the stannous fluoride toothpaste after coffee staining for natural teeth (2.6 ΔE00⁎) and composite specimens (3.1 ΔE00⁎). Coffee staining resulted in significant changes for ΔE00⁎ values of all groups, except for natural teeth brushed with a novamin-based (p= 0.06) toothpaste. For composite specimens, only the stannous fluoride and cetylpyridinium chloride-based toothpastes resulted in significant color changes relative to the control group. ΔL⁎ and Δb⁎ values were also increased after staining for all groups (p<0.05). Conclusions. Desensitizing toothpastes alone did not cause perceptible color changes; however, in combination with coffee staining, it tended to increased discoloration for both composites and natural teeth.

scholarly journals Comparative SEM evaluation of penetration of adhesive systems in human dentin with a non-rinse conditioner and a self-etching primer

2004 ◽  
Vol 15 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Julio Orrico de Aragão Pedra e Cal-Neto ◽  
Mauro Sayão de Miranda ◽  
Katia Regina Hostílio Cervantes Dias
Keyword(s):  
Penetration Depth ◽  
Third Molar ◽  
Adhesive System ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Human Tooth ◽  
Control Group ◽  
Human Teeth ◽  
Homogeneous Groups

The purpose of this study was to compare the effect of a self-etching primer and a non-rinse conditioner with the effect of a conventional adhesive system on the penetration depth in dentin of human teeth, using scanning electronic microscopy (SEM). Fifteen human third molar teeth were sectioned into 2 pieces. All pieces were flattened with grade 600 to 1200 silicon carbide paper and divided into 6 groups: group 1 - Prime & Bond NT (NT), negative control; group 2 - 37% phosphoric acid + Prime & Bond NT (PANT), positive control; group 3 - Non-rinse Conditioner (NRC) + Prime & Bond NT (NRCNT); group 4 - NRC + Prime & Bond 2.1 (NRCPB); group 5 - NRC + Scotchbond MP (NRCSB); group 6 - Prompt L-Pop (PLP). All teeth were covered with Dyract AP. The tooth fragments were decalcified, and its resin replicas were evaluated by SEM by three previously standardized examiners. The penetration was scored from 0 (no penetration) to 3 (maximum penetration). The Kruskal-Wallis and Mann-Whitney U tests (p<0.05) showed three statistically homogeneous groups: {NT, NRCPB, NRCSB and PLP}, {NRCNT} and {PANT}. The authors concluded that the self-etching primer and the non-rinse conditioner provide a lower penetration depth in human tooth dentin than the conventional adhesive system.

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