scholarly journals تثبيط التغيرات الخلوية الوراثية المستحثة بعقار MMC في الفأر الأبيض باستخدام مستخلصي نبات القريص نوع Urtica pilulifera

2007 ◽  
Vol 1 (1) ◽  
pp. 37-51
Author(s):  
Ismail Kadhum Shubber ◽  
Laith Abdel Hassan ◽  
Saadi Hamad Hilal
Keyword(s):  
Bone Marrow ◽  
Cell Division ◽  
Plant Extract ◽  
Chromosomal Aberrations ◽  
Leaf Extract ◽  
Marrow Cell ◽  
Ethanolic Extract ◽  
Genotoxic Effects ◽  
Control Drug ◽  
Cytogenetic Effects

The aim of this study was to investigate the potency of aquatic & alcoholic extracts of the leaves of Urtica pilulifera plant against mutagenecity of Mitomycine C (MMC) drug in mice.The genotoxic effects of three different concentrations 0.01, 0.1 & 0.5 mg/kg from aqueous or ethanolic extract were tested in mice. 0.01 mg/kg bw from either types of extracts were selected as non-genotoxic in comparison to control. Drug-plant extract interaction was tested using mitotic index & Chromosomal aberrations as bioindicators for detection of the potency of the plant extract in reduction of MMC-induction of cytogenetic effects. Two types of treatments were followed, either plant extracts were given before (2, 4, 6) days, or after (2, 4, 6) days, of treatment with MMC. The results revealed that 0.2 mg/kg of MMC significantly inhibited bone marrow cell division and increased the spontaneous levels of chromosomal aberration up to 20 times. Treatment of mice with aqueous or alcoholoic leaf-extract reduced significantly (p < 0.01) the genotoxic effects of MMC. This reduction was more pronounced in animals which were given the extract after treatment with MMC comparing to its treatment before the MMC exposure. These results indicated that Utrtica pilulifera leaves extract behave as bio-anti mutagen first degree and acting indirectly in the 2nd degree.

scholarly journals Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

2019 ◽  
Vol 6 (01) ◽  
pp. e7-e14 ◽  
Author(s):  
Micena Alves e Silva ◽  
Cinthia Pacheco ◽  
Mila Madeira ◽  
Adriana Saraiva ◽  
Elisângela de Freitas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Ethyl Acetate ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Ethanolic Extract ◽  
Quinic Acid ◽  
Ethyl Acetate Fraction ◽  
Raw 264.7 ◽  
Hancornia Speciosa

Abstract Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-(+)-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis.

Bone marrow cell chromosomal aberrations and styrene biotransformation in mice given styrene on a repeated oral schedule

1983 ◽  
Vol 45 (3) ◽  
pp. 349-357 ◽  
Author(s):  
Isabella Sbrana ◽  
Donella Lascialfari ◽  
Anna Maria Rossi ◽  
Nicola Loprieno ◽  
Marina Bianchi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Chromosomal Aberrations ◽  
Marrow Cell

scholarly journals Evaluation of Some Hematological and Cytogenetic Effects of Ammi majus Seed Aqueous Extract in Albino Male Mice

2010 ◽  
Vol 4 (1) ◽  
pp. 104-110
Author(s):  
Rakad M. Kh. Al-Jumaily ◽  
Ali H. Ad'hiah ◽  
Mohammed M. F. Al. Halbosiy ◽  
Baraa A. Abdul Hameed
Keyword(s):  
Mitotic Index ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Chromosomal Aberrations ◽  
Post Treatment ◽  
Male Mice ◽  
Spontaneous Formation ◽  
Cytogenetic Effects ◽  
Ammi Majus ◽  
Mutagenic Effects

The present study was carried out with the aim to evaluate the hematological and cytogenetic effects of seed aqueous extract of the plant Ammi majus (0.5, 1.0, 1.5) mg/kg in albino male mice. The investigated parameters were total count of leucocytes (TLC), mitotic index (MI), micronucleus (MN) formation and chromosomal aberrations. The mitomycin C (MMC) was used as a mutagen in the interaction with the plant extract (pre- and post-treatment), with the aim to determine the antimutagenic efficiency of the plant extract, and in all cases, the materials were given orally. In the first treatment, the results indicated that the dose 1.5 mg/kg of the extract enhanced the parameters investigated and a significant increase was observed in TLC (10070 cells/cu.mm.blood) as compared to negative (7290 cells/cu.mm.blood) or positive (4910 cells/cu.mm.blood) controls, and such observation was positively correlated with the mitotic index. In contrast, the spontaneous formation of MN was significantly decreased in the three investigated doses of the extract. In pre- and post-treatment experiments, a similar picture was drawn, and the plant extract was able to modulate the mutagenic effects of MMC.

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