scholarly journals The Effect of Sperm Preparation Medium on Malondialdehyde Concentration in the Seminal Plasma of Normozoospermic and Oligoastheno- teratozoospermic patient

2009 ◽  
Vol 3 (2) ◽  
pp. 74-79
Author(s):  
Maha Khalid Abd-Al Kareem
Keyword(s):  
Seminal Plasma ◽  
The Other ◽  
Oxygen Species ◽  
Sperm Preparation ◽  
Fertilization Rates ◽  
Oxidative Damages ◽  
Infertile Men ◽  
Acrosomal Reaction ◽  
Before And After ◽  
Intra Uterine Insemination

Intra uterine insemination (IUI) has been widely used to treat infertility. Infertility may be due to increased levels of reactive oxygen species (ROS) in the seminal plasma. The goal of our study was to confirm that using sperm preparation medium to activate the spermatozoa in IUI might be helpful to decrease the levels of ROS in the seminal plasma, lowering the oxidative stress and the damage effects of the sperm and enhance a good quality sperm capable of capacitation, acrosomal reaction and fertilization. The levels of malondialdehyde (MDA) were measured in the seminal plasma in 40 infertile men of normozoospermia and oligoastheno-teratozoospermia before and after adding medium to the samples. Results showed that there was a significant (P<0.05) decrease in all parameters of semen samples in oligoastheno-teratozoosperia as compared with the other group, there was no significant increase in the level of MDA before adding the medium to the samples and a significant (P<0.05) increase after adding the medium to the same samples in the second group as compared with normozoospermic patients. Results reported in this study, clearly indicated that adding sperm preparation medium to the semen samples would improve the fertilization rates by decreasing the oxidative damages to the spermatozoa. Our results might be need a further studies to find a clue for the treatment of infertility in man.

UP-2.047: Reactive Oxygen Species (ROS) Level in Seminal Plasma of Infertile Men and Healthy Donors

Urology ◽  
2009 ◽  
Vol 74 (4) ◽  
pp. S246-S247 ◽  
Author(s):  
M. Moein ◽  
V. Dehghani ◽  
N. Tabibnejad ◽  
S. Vahidi
Keyword(s):  
Reactive Oxygen Species ◽  
Seminal Plasma ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Infertile Men ◽  
Healthy Donors

scholarly journals Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 539 ◽  
Author(s):  
Marion Papas ◽  
Jaime Catalán ◽  
Beatriz Fernandez-Fuertes ◽  
Laura Arroyo ◽  
Anna Bassols ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Seminal Plasma ◽  
Specific Activity ◽  
Routine Practice ◽  
Oxygen Species ◽  
Sperm Cryopreservation ◽  
Before And After ◽  
Specific Activities ◽  
Plasma Antioxidant

While the removal of seminal plasma is a routine practice prior to equine sperm cryopreservation, this fluid contains the main source of antioxidant enzymes able to scavenge these reactive oxygen species. Therefore, stallion seminal plasma components may have an impact on ejaculate freezability. Against this background, this study was designed to investigate whether the activities of the main stallion seminal plasma antioxidant enzymes are related to sperm cryotolerance. With this purpose, 16 ejaculates were collected from 14 healthy stallions, and each ejaculate was split into two aliquots. The first one was used to evaluate the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GSR) in seminal plasma. The second aliquot was extended and then processed for cryopreservation. Sperm motility and viability were evaluated before and after cryopreservation, and ejaculates were classified as of good (GFE) or poor freezability (PFE) based on total motile and viable spermatozoa at post-thaw. We observed that, while the specific activities of CAT, GPX, and GSR were similar between GFE and PFE, that of SOD was significantly (p < 0.05) higher in GFE than in PFE. We can thus conclude that, in stallions, the specific activity of SOD in the seminal plasma of a given ejaculate might be related to its freezability.

232 INFLUENCE OF SEMINAL PLASMA ON THE SUSCEPTIBILITY OF DOG SPERM AGAINST DIFFERENT REACTIVE OXYGEN SPECIES

2011 ◽  
Vol 23 (1) ◽  
pp. 215
Author(s):  
A. Dalmazzo ◽  
P. A. A. Góes ◽  
M. Nichi ◽  
R. O. C. Silva ◽  
J. R. C. Gurgel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Hydroxyl Radical ◽  
Seminal Plasma ◽  
Superoxide Anion ◽  
The Other ◽  
Mitochondrial Activity ◽  
Oxygen Species ◽  
Acrosome Integrity

Due to the importance of dogs to humans, there is increasing interest in breeders in the use of reproductive biotechnologies. However, most of the biotechnologies would require the removal or dilution of the seminal plasma, which is known to exert both beneficial and deleterious effects on sperm quality. One of the beneficial effects of seminal plasma would be the antioxidant protection because sperm are particularly susceptible to oxidative stress, mainly due to the reduced cytoplasm and the high content of polyunsaturated fatty acids in their membrane. An alternative to overcome the injuries caused by oxidative stress is the antioxidant treatment, which requires the identification of those reactive oxygen species (ROS) that are the most deleterious. The aim of this study was to identify the most harmful ROS to dog semen. Semen samples from 6 adult dogs were collected and centrifuged. Seminal plasma (SP) was removed and samples were incubated (1 h, 37°C) with 4 ROS-inducing mechanisms: xanthine/xanthine oxidase (produces superoxide anion), hydrogen peroxide (4 mM), ascorbate and ferrous sulfate (4 mM; produces hydroxyl radical) alone or with additional SP. Samples were analysed for motility by computer assisted sperm analysis (CASA). The 3-3′ diaminobenzidine stain was used as an index of mitochondrial activity, the eosin nigrosin stain as an index of membrane integrity, the simple stain (fast green/Bengal rose) as an index of acrosome integrity, sperm chromatin structure assay (SCSA) as an index of DNA fragmentation, and measurement of thiobarbituric acid reactive substances (TBARS) as an index of lipid peroxidation. Statistical analysis was performed using the SAS System for Windows (SAS Institute Inc., Cary, NC, USA; least significant differences test and Spearman correlation; P < 0.05). Results showed that dog sperm is differentially modulated depending on the presence of SP. In addition, damage to the different sperm structures depended on the different ROS. Samples incubated with SP showed no differences concerning TBARS (1 233 in SP, 1 260 in Tris; P = 0.99). On the other hand, samples incubated without SP showed higher lipid peroxidation when treated with hydroxyl radical compared with the other ROS. Furthermore, although hydroxyl radical mostly altered mitochondrial activity in samples incubated with SP (DAB IV = 4.3%; P < 0.05 against all other ROS), the most significant ROS in samples incubated without SP was hydrogen peroxide (DAB IV = 4.7%; P < 0.05 against all other ROS). Superoxide anion was less harmful to acrosome integrity in samples incubated with SP and to motility in samples incubated without SP. The present results suggest that seminal plasma may play an important role in the susceptibility of dog sperm to oxidative stress. Moreover, the results indicate that different sperm compartments are susceptible to different ROS. It is concluded that the quality of frozen–thawed dog semen may be improved by treating with a combination of different antioxidants to destroy the chain reaction causing the oxidative stress. FAPESP is acknowledged for financial support.

scholarly journals Seminal Plasma Clusterin as a Biomarker for Spermatogenesis in Varicocele Patients before and after Varicocelectomy

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
H M A Saleh ◽  
A A Afify ◽  
W A Ahmed ◽  
M S Daruish
Keyword(s):  
Seminal Plasma ◽  
Sperm Count ◽  
Semen Analysis ◽  
Progressive Motility ◽  
Infertile Men ◽  
Pampiniform Plexus ◽  
Significant Difference ◽  
Before And After ◽  
Sperm Development ◽  
High Concentrations

Abstract Introduction a varicocele is an abnormally dilated pampiniform plexus of veins. Hyperthermia, oxidative stress, and elevated apoptotic index have been described in men with variocele and infertility. Clusterin (Clu) is a chaperone-like glycoprotein which is synthesized by Sertoli cells in high concentrations and is deposited onto the membranes of and mature spermatozoa, giving rise to the possibility that it has a role in sperm development. Secreted form of clusterin is a powerful anti-apoptotic. Aim To explore clusterin level in seminal plasma of infertile men with varicocele in comparison to normal healthy fertile subjects and to compare the seminal plasma clusterin level before and after varicocelectomy Methods The study included 25 patients suffering from varicocele with infertility (whether primary or secondary) for 12 months, and 10 healthy volunteers of matched age and sex as control. For all the patients Sub-inguinal varicocelectomy was done. Semen analysis, and seminal plasma clusterin assay were carried out before and three months after varicocelectomy. Results there was a statistically significant difference between patients and controls as regards sperm count, progressive motility, and seminal plasma clusterin (P &lt; 0.01). Three months after varicocelectomy, the sperm count had a highly significant improvement in the patient’s group (Mean= 17.90 + 8.86) compared to a preoperative count (Mean= 11.43 + 7.56). There was a highly significant improvement in post-operative progressive motility in the patient’s group (Mean= 34.5 + 20.2) compared to a preoperative progressive motility (Mean= 22.05 + 15.5). Post-operative clusterin showed a highly significant elevation in the patient’s group postoperative (Mean= 10.73 + 4.5) compared to a preoperative clusterin (Mean= 6.68 + 3.8). Conclusion Based on our study we could suggest another way of contribution of varicocele to male infertility by affecting seminal plasma clusterin level.

scholarly journals Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species

2015 ◽  
Vol 12 (1) ◽  
Author(s):  
Ashok Agarwal ◽  
Ahmet Ayaz ◽  
Luna Samanta ◽  
Rakesh Sharma ◽  
Mourad Assidi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Seminal Plasma ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Infertile Men

scholarly journals Abnormal expression of acid glycosidases in seminal plasma and spermatozoa from infertile men with varicocele

Reproduction ◽  
2002 ◽  
pp. 411-417 ◽  
Author(s):  
JJ Corrales ◽  
RM Burgo ◽  
P Galindo ◽  
I Munoz-Barroso ◽  
JM Miralles ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Soluble Fraction ◽  
The Other ◽  
Semen Parameters ◽  
Obstructive Azoospermia ◽  
Infertile Men ◽  
Normal Fertility ◽  
Beta Galactosidase ◽  
Infertile Patients

The activities of acid beta-glucuronidase, alpha-mannosidase, alpha-glucosidase, alpha-galactosidase, beta-galactosidase and beta-N-acetylglucosaminidase were analysed in seminal plasma and spermatozoa from 26 infertile men with varicocele and from 36 men of normal fertility. Semen samples from ten men with non-obstructive azoospermia were used as control specimens that contained the other components of semen. Spermatozoa were solubilized by both physical (homogenization) and chemical (Triton-X100) methods to obtain the soluble and non-soluble fractions. The activities of several glycosidases measured both in seminal plasma and spermatozoa were directly correlated with the numbers of spermatozoa and sperm motility, confirming previous studies. As some infertile patients with varicocele have normal semen parameters, whereas others have low numbers of spermatozoa and low sperm motility, the varicocele patients were prospectively divided into two groups: one (n = 15) with normal spermiograms and the other (n = 11) with abnormal spermiograms. The activities (expressed in mU ml(-1)) of alpha-mannosidase, beta-galactosidase and beta-N-acetylglucosaminidase in seminal plasma of normozoospermic infertile patients with varicocele were significantly higher than those of fertile controls, but not when expressed in U per 10(8) spermatozoa. The activities of beta-glucuronidase, alpha-mannosidase, beta-galactosidase and beta-N-acetylglucosaminidase in seminal plasma when expressed in U per 10(8) spermatozoa in varicocele patients with abnormal spermiograms were significantly higher than in those of men of normal fertility. The activity of alpha-mannosidase in the soluble fraction of sperm homogenates, expressed as U per 10(8) spermatozoa, was significantly higher in infertile patients with varicocele and abnormal spermiograms than in controls. In the non-soluble fraction of spermatozoa from infertile patients with varicocele, there was an increase in the expression of beta-galactosidase and beta-N-acetylglucosaminidase activities compared with the fraction of spermatozoa from fertile subjects. In summary, infertile patients with varicocele displayed an overexpression of acid alpha-mannosidase, beta-galactosidase and beta-N-acetylglucosaminidase activities in seminal plasma and spermatozoa that may be associated with functional defects in spermatozoa as these glycosidases play an important role in mammalian fertilization.

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