Sero-prevalence of bovine Leukemia virus in cattle from Caquetá state, Colombia

Bovine Leukemia Virus (BLV) is the agent of enzootic bovine leukosis (EBL), this disease is a neoplasm of lymphatic tissue in bovine species. The aim of this article was determinate the prevalence of bovine leukemia virus in dual-purpose cattle from nine municipalities that produce the 98% of milk in the Caquetá state, Colombia. Blood samples were collected in 100 herds dedicates to dual purpose cattle, obtained blood serum from 1000 animals, of which 893 corresponding to cows. Indirect Elisa test for detection of antibodies anti-GP51 of BLV was performance and the positive cases were considered if the serum-to-positive ratio with percentages of M/N lower than 40%. Categorized data were analyzed by contingency tables and ANOVA at the significant level of p<0.05 by DGC test was performed. The overall sero-prevalence of BLV in Caquetá state was of 25.18% (95%, CI: 21.9-28.46%), in males 26.25% and females 25.37% respectively. At municipal level the sero-prevalence varied of 7.12 to 41.81%. The prevalence of BLV at herd level was of 67% (95%, CI: 57.24-76.76%). In conclusion, the sero-prevalence of BLV in the dual-purpose livestock system over 36 months of age in Caquetá state is moderate, do not exist statistical difference between sero-prevalence of cows and bulls. At level of herds the prevalence of BLV is high. Improving strategies of control and managements in the herds, as well as implement policies of sanitary management are necessary.

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Distribution of bovine leukemia virus proviral DNA sequences in tissues of animals with enzootic bovine leukosis

Leukemia Research ◽

10.1016/0145-2126(78)90004-8 ◽

1978 ◽

Vol 2 (1) ◽

pp. 23-32 ◽

Cited By ~ 26

Author(s):

Richard Kettmann ◽

Arsène Burny ◽

Yvette Cleuter ◽

Jacques Ghysdael ◽

Marc Mammerickx

Keyword(s):

Dna Sequences ◽

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Enzootic Bovine Leukosis ◽

Proviral Dna ◽

Bovine Leukosis

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Enzootic Bovine Leukosis and Bovine Leukemia Virus

Gene Expression in Normal and Transformed Cells ◽

10.1007/978-1-4684-4541-1_11 ◽

1983 ◽

pp. 231-245

Author(s):

G. Marbaix ◽

R. Kettmann ◽

J. Deschamps ◽

D. Couez ◽

M. Mammerickx ◽

...

Keyword(s):

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Enzootic Bovine Leukosis ◽

Bovine Leukosis

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Bovine leukemia virus tax gene/Tax protein polymorphism and its relation to Enzootic Bovine Leukosis

Virulence ◽

10.1080/21505594.2019.1708051 ◽

2019 ◽

Vol 11 (1) ◽

pp. 80-87 ◽

Cited By ~ 3

Author(s):

Irina M. Zyrianova ◽

Svetlana N. Kovalchuk

Keyword(s):

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Protein Polymorphism ◽

Enzootic Bovine Leukosis ◽

Tax Protein ◽

Bovine Leukosis

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MASKED GENOTYPE 6 OF BOVINE LEUKEMIA VIRUS IS FOUND IN COLOMBIAN CATTLE

10.21203/rs.2.11155/v1 ◽

2019 ◽

Author(s):

Adriana Patricia Corredor-Figueroa ◽

Nury Nathalia Olaya-Galán ◽

Sandra Patricia Salas ◽

Juan Sebastián Quintero ◽

Álvaro Fajardo ◽

...

Keyword(s):

Genetic Variability ◽

Bovine Leukemia Virus ◽

Phylogenetic Analyses ◽

Leukemia Virus ◽

Gene Analysis ◽

Economic Losses ◽

Envelope Gene ◽

Genotype 1 ◽

Global Epidemiology ◽

Bovine Leukosis

Abstract Background Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. Results A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. Conclusions This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities.

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Bovine leukosis and the possibility to cause cancer in humans: A scientific review

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v42i1.31 ◽

2018 ◽

Vol 42 (1) ◽

pp. 52-60

Author(s):

Huda Hameed Kadhim Alabbody

Keyword(s):

Bovine Leukemia Virus ◽

Human Cancer ◽

Leukemia Virus ◽

Cell Leukemia ◽

Scientific Review ◽

Enzootic Bovine Leukosis ◽

Human T Cell ◽

T Cell Leukemia Virus ◽

Bovine Leukosis ◽

Control And Eradication

This review was made to explore the recent multiple studies on enzootic bovine leukosis, focusing on its prevalence, economic impact, the link with public health and the possibility to cause cancer in humans. The causative agent of enzootic bovine leukosis is a virus closely related to human T- cell leukemia virus (HTLV-1). The closeness between the two viruses helps the progress of cancer research in diagnosis and treatment, also the development of a vaccine in both human and veterinary medicine .The enzootic bovine leukosis is widely spread in the continents. The economic loses of enzootic bovine leukosis is related to the lowered productivity of effected cattle, morbidity, mortality and cost of control and eradication. This review proved that bovine leukemia virus is innocent from human cancer infection and there is no proof of virus living in human tissues. But this subject needs a lot of research to know the mechanism of the virus and its affects in cellular content of the organism.

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First Report of Bovine Leukemia Virus Infection in Yaks (Bos mutus) in China

BioMed Research International ◽

10.1155/2016/9170167 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4 ◽

Cited By ~ 6

Author(s):

Jian-Gang Ma ◽

Wen-Bin Zheng ◽

Dong-Hui Zhou ◽

Si-Yuan Qin ◽

Ming-Yang Yin ◽

...

Keyword(s):

Risk Factors ◽

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Northwest China ◽

Gansu Province ◽

Serum Samples ◽

First Report ◽

Elisa Kit ◽

Bovine Leukosis ◽

Positive Results

Enzootic bovine leukosis (EBL) is a chronic lymphosarcoma disease of cattle caused by bovine leukemia virus (BLV). No information is available concerning the epidemiology of BLV infection in yaks (Bos mutus). One thousand five hundred and eighty-four serum samples from 610 black yaks and 974 white yaks from Gansu province, northwest China, were collected between April 2013 and March 2014 and tested for BLV antibodies using a commercially available ELISA kit. The overall BLV seroprevalence in yaks was 21.09% (334/1584), with 24.26% (148/610) black yaks and 19.10% (186/974) white yaks yielding positive results. Risk factor analysis indicated that with the exception of breed (OR = 1.36, 95% CI = 1.06–1.73,P<0.05), the age, region, gender, farm, and the numbers of pregnancies were not considered as risk factors for the presence of BLV in yaks included in this study. This is the first report of BLV infection in yaks in China, which provides information for controlling BLV infection in yaks.

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Molecular epidemiology of bovine leukemia virus associated with enzootic bovine leukosis in Japan

Virus Research ◽

10.1016/j.virusres.2010.11.005 ◽

2011 ◽

Vol 155 (1) ◽

pp. 343-348 ◽

Cited By ~ 26

Author(s):

Keiko Matsumura ◽

Emi Inoue ◽

Yoshiaki Osawa ◽

Katsunori Okazaki

Keyword(s):

Molecular Epidemiology ◽

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Enzootic Bovine Leukosis ◽

Bovine Leukosis

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Tumor necrosis factor-alpha (TNFα) gene polymorphism and expression of membrane-bound TNFα protein on CD11b+ and IgM+ cells in cows naturally infected with bovine leukemia virus

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2015-0069 ◽

2015 ◽

Vol 18 (3) ◽

pp. 533-539

Author(s):

B. Bojarojć-Nosowicz ◽

E. Kaczmarczyk ◽

A. Stachura ◽

M. Kubińska

Keyword(s):

Bovine Leukemia Virus ◽

Promoter Region ◽

Leukemia Virus ◽

Necrosis Factor Alpha ◽

Membrane Bound ◽

Factor Alpha ◽

Cell Subpopulations ◽

Bovine Leukosis ◽

First Time ◽

Necrosis Factor

Abstract The aim of this study was to determine whether SNP at position -824 (promoter region) of the TNFα gene significantly differentiates the size of IgM+, CD5+ and CD11b+ cell subpopulations and affects the expression of membrane-bound TNFα protein (mTNFα) on these cells and their susceptibility to BLV infections. In this study, significant differences were determined for the first time between TNFα genotypes and the percentage of cells with the CD11b+TNFα+p24+ immunophenotype. Furthermore, greater expansion of lymphocytes with the IgM+TNFα+p24+ immunophenotype was reported in cows with the G/G genotype than in A/A homozygotes. Cells with the above immunophenotype were more frequently observed in cows with persistent leukocytosis than in aleukemic cattle. Our results suggest that polymorphism of the TNFα-824 A>G gene and mTNFα protein expression play an important role in the pathogenesis of enzootic bovine leukosis.

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