scholarly journals Reuse of polluting agroindustrial waste for ethanol production by Kluyveromyces marxianus

2019 ◽  
pp. 195-199 ◽  
Author(s):  
Joselma Ferreira da Silva ◽  
Naiara Priscila Silva Reis Barbosa ◽  
Matheus Tavares do Nascimento França ◽  
Laureen Michelle Houllou ◽  
Carolina Barbosa Malafaia
Keyword(s):  
Ethanol Production ◽  
Kluyveromyces Marxianus ◽  
Crude Glycerol ◽  
Cheese Whey ◽  
Low Cost ◽  
Dairy Industry ◽  
Alternative Source ◽  
Agroindustrial Residues ◽  
Agroindustrial Waste ◽  
Low Cost Substrate

The development of research for the production of biofuels using low cost substrate has become more relevant in recent years. These include reuse of residues such as crude residual glycerol from biodiesel (CRG) and cheese whey (CW) from the dairy industry. The present work evaluated the ethanol production by isolates of the yeast Kluyveromyces marxianus using agroindustrial residues as an alternative source of carbon. The cultures were rotated 100 rpm at 30 ° C for 24 h. The ethanol production was observed in both media, however, in the CW higher values of ethanol were observed in relation to the CRG. The results showed that K. marxianus isolates were adapted to the use of lactose present in cheese whey as a source of carbon for the production of ethanol with concentrations ranging from 11.41 to 19.9 g.L-1, but did not demonstrate efficiency in the use of crude glycerol for this purpose

scholarly journals Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

2020 ◽  
Author(s):  
Feng Zhao ◽  
Chao Guo ◽  
Qingfeng Cui
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Practical Significance ◽  
Glycerol Metabolism ◽  
Metabolic Intermediate ◽  
Spectra Analysis ◽  
Low Cost Substrate ◽  
Anaerobic Production

Abstract Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

scholarly journals Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

2021 ◽  
Author(s):  
Feng Zhao ◽  
Mengyao Zheng ◽  
Qingfeng Cui
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Practical Significance ◽  
Glycerol Metabolism ◽  
Metabolic Intermediate ◽  
Spectra Analysis ◽  
Low Cost Substrate ◽  
Anaerobic Production

Abstract Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

Ethanol production from deproteinized cheese whey fermentations by co-cultures of Kluyveromyces marxianus and Saccharomyces cerevisiae

2013 ◽  
Vol 7 (13) ◽  
pp. 1121-1127 ◽  
Author(s):  
Karina Teixeira Magalhães-Guedes ◽  
◽  
Ana Karla Rodrigues ◽  
Ivani Maria Gervasio ◽  
Ivaldesa Gervasio ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Kluyveromyces Marxianus ◽  
Cheese Whey ◽  
Deproteinized Cheese Whey

Biodiesel derived crude glycerol and tuna condensate as an alternative low-cost fermentation medium for ethanol production by Enterobacter aerogenes

2019 ◽  
Vol 138 ◽  
pp. 111451 ◽  
Author(s):  
Juli Novianto Sunarno ◽  
Poonsuk Prasertsan ◽  
Wiriya Duangsuwan ◽  
Benjamas Cheirsilp ◽  
Kanokphorn Sangkharak
Keyword(s):  
Ethanol Production ◽  
Crude Glycerol ◽  
Low Cost ◽  
Enterobacter Aerogenes ◽  
Fermentation Medium

Cellulase Enzyme Production from Agricultural Waste by Acinetobacter sp. KKU44

2014 ◽  
Vol 931-932 ◽  
pp. 1106-1110 ◽  
Author(s):  
Nutt Poomai ◽  
Wilailak Siripornadulsil ◽  
Surasak Siripornadulsil
Keyword(s):  
Ethanol Production ◽  
Cellulose Degradation ◽  
Low Cost ◽  
Agricultural Waste ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Agricultural Wastes ◽  
Optimal Temperature ◽  
Cellulase Enzyme ◽  
Low Cost Substrate

Due to a high ethanol demand, the approach for effective ethanol production is important and has been developed rapidly worldwide. Several agricultural wastes are highly abundant in celluloses and the effective cellulase enzymes do exist widely among microorganisms. Accordingly, the cellulose degradation using microbial cellulase to produce a low-cost substrate for ethanol production has attracted more attention. In this study, the cellulase producing bacterial strain has been isolated from rich straw and identified by 16S rDNA sequence analysis as Acinetobacter sp. KKU44. This strain is able to grow and exhibit the cellulase activity. The optimal temperature for its growth and cellulase production is 37 °C. The optimal temperature of bacterial cellulase activity is 60 °C. The cellulase enzyme from Acinetobacter sp. KKU44 is heat-tolerant enzyme. The bacterial culture of 36 h. showed highest cellulase activity at 120 U/mL when grown in LB medium containing 2% (w/v). The capability of Acinetobacter sp. KKU44 to grow in cellulosic agricultural wastes as a sole carbon source and exhibiting the high cellulase activity at high temperature suggested that this strain could be potentially developed further as a cellulose degrading strain for a production of low-cost substrate used in ethanol production.

A critical review on microalgae as an alternative source for bioenergy production: A promising low cost substrate for microbial fuel cells

2016 ◽  
Vol 154 ◽  
pp. 104-116 ◽  
Author(s):  
Z. Baicha ◽  
M.J. Salar-García ◽  
V.M. Ortiz-Martínez ◽  
F.J. Hernández-Fernández ◽  
A.P. de los Ríos ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Critical Review ◽  
Microbial Fuel Cells ◽  
Low Cost ◽  
Alternative Source ◽  
Bioenergy Production ◽  
Low Cost Substrate
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