scholarly journals Lateral Flow Immunoassay Based Colloidal Gold Nanoparticles for the Detection of Organophosphorous Pesticide (Propanoic) in Cow’s Milk

2021 ◽  
Vol 17 (3) ◽  
pp. 1-14
Author(s):  
Azhar Haleem ◽  
Israa Haleem ◽  
Ruaa Abass
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Organophosphorus Pesticide ◽  
Lateral Flow ◽  
Cow’S Milk ◽  
Lateral Flow Immunoassay ◽  
Detection Zone ◽  
Cow's Milk ◽  
Milk Samples ◽  
Colloidal Gold Nanoparticles

Simple, inexpensive, and high sensitive Lateral Flow Immunoassay (LFI) based colloidal gold nanoparticles antigen-coated as the label was designed for the fast determination of organophosphorus pesticide in cow’s milk samples. The developed LFI detection strip consisted of four structural zones start from the sample pad, conjugate pad, detection zone, and finally absorbent pad. Immune-sensitive zone was synthesized and functionalized with Propanoic Hapten–OVA, Goat monoclonal-anti-Propanoic antibodies were produced. Propionic -cationized ovalbumin-HPR conjugates were fabricated and identified. The antibody optimal conditions were set and the influence of pH on the analytical properties of the antibody-based gold nanoparticles was also investigated. The detection limit of the LFI for Propanoic standard into milk samples were 0.5 and 100 ng/mL, respectively. The test development time for the LFI was less than 5 min, suitable for fast on-site measuring of Propanoic.

Development and Validation of a Lateral Flow Immunoassay Test Kit for Dual Detection of Casein and β-Lactoglobulin Residues

2016 ◽  
Vol 79 (3) ◽  
pp. 477-483 ◽  
Author(s):  
JONGKIT MASIRI ◽  
BRIANDA BARRIOS-LOPEZ ◽  
LORA BENOIT ◽  
JOSHUA TAMAYO ◽  
JEFFREY DAY ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Test ◽  
Lateral Flow ◽  
Test Method ◽  
Cow’S Milk ◽  
Lateral Flow Immunoassay ◽  
Screening Methods ◽  
Cow's Milk ◽  
Test Kit ◽  
Β Lactoglobulin

ABSTRACT Allergies to cow's milk are very common and can present as life-threatening anaphylaxis. Consequently, food labeling legislation mandates that foods containing milk residues, including casein and/or β-lactoglobulin, provide an indication of such on the product label. Because contamination with either component independent of the other can occur during food manufacturing, effective allergen management measures for containment of milk residues necessitates the use of dual screening methods. To assist the food industry in improving food safety practices, we have developed a rapid lateral flow immunoassay test kit that reliably reports both residues down to 0.01 μg per swab and 0.1 ppm of protein for foods. The assay utilizes both sandwich and competitive format test lines and is specific for bovine milk residues. Selectivity testing using a panel of matrices with potentially interfering substances, including commonly used sanitizing agents, indicated reduction in the limit of detection by one-to fourfold. With food, residues were easily detected in all cow's milk–based foods tested, but goat and sheep milk residues were not detected. Specificity analysis revealed no cross-reactivity with common commodities, with the exception of kidney beans when present at high concentrations (>1%). The development of a highly sensitive and rapid test method capable of detecting trace amounts of casein and/or β-lactoglobulin should aid food manufacturers and regulatory agencies in monitoring for milk allergens in environmental and food samples.

Adulteration of cow’s milk with buffalo’s milk detected by an on-site carbon nanoparticles-based lateral flow immunoassay

2021 ◽  
Vol 351 ◽  
pp. 129311
Author(s):  
Rajan Sharma ◽  
Archana Verma ◽  
Nitin Shinde ◽  
Bimlesh Mann ◽  
Kamal Gandhi ◽  
...  
Keyword(s):  
Carbon Nanoparticles ◽  
Lateral Flow ◽  
Cow’S Milk ◽  
Lateral Flow Immunoassay ◽  
Cow's Milk

Dense aqueous colloidal gold nanoparticles prepared from highly concentrated precursor solution

2011 ◽  
Vol 362 (2) ◽  
pp. 325-329 ◽  
Author(s):  
Tetsuro Soejima ◽  
Seisaku Oshiro ◽  
Yasuji Nakatsuji ◽  
Seishiro Ito
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Precursor Solution ◽  
Colloidal Gold Nanoparticles

An enhanced ELISA based on modified colloidal gold nanoparticles for the detection of Pb(II)

2011 ◽  
Vol 26 (8) ◽  
pp. 3700-3704 ◽  
Author(s):  
Yu Zhou ◽  
Xiang-Li Tian ◽  
Yan-Song Li ◽  
Feng-Guang Pan ◽  
Yuan-Yuan Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Colloidal Gold Nanoparticles

Effect of Voltage on Colloidal Gold (Au) Nanoparticles Produced Using Electro-Dissolution-Reduction Method

2015 ◽  
Vol 1115 ◽  
pp. 386-389
Author(s):  
Haroon Haiza ◽  
I.I. Yaacob ◽  
Ahmad Zahirani Ahmad Azhar
Keyword(s):  
Gold Nanoparticles ◽  
Electron Microscope ◽  
Colloidal Gold ◽  
Reduction Process ◽  
Chloride Ions ◽  
Complex Ions ◽  
Dc Power ◽  
Colloidal Gold Nanoparticles ◽  
Transmission Electron ◽  
The Mean

Colloidal gold nanoparticles have been successfully prepared using a simple two-electrode cells connected to a DC power supply. During the electro-dissolution-reduction process, the bulk gold at the anode oxidized into gold cations which then reacted with the chloride ions to form aurochloride complex. The complex ions were then reduced by the citrate ion to form colloidal gold nanoparticles. The size and shape of the nanoparticles were modulated by varying the terminal voltages. The colloidal gold nanoparticles obtained were characterized by field-emission scanning electron microscope (FESEM), transmission electron microscope (TEM) and ultraviolet-visible spectrophotometer (UV-Vis). From FESEM analysis, it was found that by increasing the voltage, the size of colloidal gold nanoparticles produced marginally decreased. The mean sizes of gold nanoparticles were roughly about 23.5 nm, 23.2 nm and 19.3 nm for 32 V, 36 V and 40 V, respectively. TEM micrograph showed that the shape of gold nanoparticles obtained is almost spherical. The characteristic peaks of UV-Vis spectra revealed that the suspension was indeed colloidal gold nanoparticles. Keywords: Gold, Nanoparticles, Electro-dissolution-reduction

Human Breast Milk Contains Bovine lgG. Relationship to Infant Colic?

PEDIATRICS ◽  
1991 ◽  
Vol 87 (4) ◽  
pp. 439-444
Author(s):  
Patrick S. Clyne ◽  
Anthony Kulczycki
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Milk Protein ◽  
Cow’S Milk ◽  
Human Breast Milk ◽  
Human Breast ◽  
Cow's Milk ◽  
Milk Samples ◽  
Infant Colic ◽  
Β Lactoglobulin

Previous studies have suggested that an unidentified cow's milk protein, other than β-lactoglobulin and casein, might play a pathogenetic role in infant colic. Therefore, a radioimmunoassay was used to analyze human breast milk and infant formula samples for the presence of bovine IgG. Milk samples from 88 of the 97 mothers tested contained greater than 0.1 µg/mL of bovine IgG. In a study group of 59 mothers with infants in the colic-prone 2- to 17-week age group, the 29 mothers of colicky infants had higher levels of bovine IgG in their breast milk (median 0.42 µg/mL) than the 30 mothers of noncolicky infants (median 0.32 µg/mL) (P < .02). The highest concentrations of bovine IgG observed in human milk were 8.5 and 8.2 µg/mL. Most cow's milk-based infant formulas contained 0.6 to 6.4 µg/mL of bovine IgG, a concentration comparable with levels found in many human milk samples. The results suggest that appreciable quantities of bovine IgG are commonly present in human milk, that significantly higher levels are present in milk from mothers of colicky infants, and that bovine IgG may possibly be involved in the pathogenesis of infant colic.

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