scholarly journals In-Vitro Evaluation of Trichoderma Isolates Against Major Soil Borne Pathogens in Groundnut (Arachis Hypogaea L)

2017 ◽  
Vol 7 (3) ◽  
pp. 319-326
Author(s):  
A. Ranga Rani et al., A. Ranga Rani et al., ◽  
Keyword(s):  
Arachis Hypogaea ◽  
In Vitro Evaluation ◽  
Soil Borne Pathogens ◽  
Arachis Hypogaea L ◽  
Trichoderma Isolates

Direct in vitro Regeneration of Arachis Hypogaea L.(Jl-24) and Study of Salt Tolerance

2011 ◽  
Vol 3 (8) ◽  
pp. 52-54 ◽  
Author(s):  
Bhagya Lakshmi Jyothi Kusuma ◽  
◽  
Sharmila Begum S
Keyword(s):  
Salt Tolerance ◽  
Arachis Hypogaea ◽  
In Vitro Regeneration ◽  
Arachis Hypogaea L

scholarly journals In Vitro Callus Culture and Plant Regeneration from Different Explants of Groundnut(Arachis hypogaea L.).

1996 ◽  
Vol 46 (4) ◽  
pp. 315-320
Author(s):  
Perumal Venkatachalam ◽  
Adaikalam Subramaniampillai ◽  
Narayanasamylpillai Jayabalan
Keyword(s):  
Plant Regeneration ◽  
Arachis Hypogaea ◽  
Callus Culture ◽  
Arachis Hypogaea L

scholarly journals In-vitro Evaluation of Bio-control agents against Soil Borne Plant Pathogens

2019 ◽  
Vol 5 ◽  
pp. 68-72
Author(s):  
Shrinkhala Manandhar ◽  
Bimala Pant ◽  
Chetana Manandhar ◽  
Suraj Baidya
Keyword(s):  
Plant Pathogens ◽  
Field Experiments ◽  
Colony Growth ◽  
Dual Culture ◽  
Vitro Assay ◽  
Trichoderma Species ◽  
Soil Borne Pathogens ◽  
Trichoderma Sp ◽  
Trichoderma Isolates

Biocontrol is an important aspect of disease management for plant pathogens, especially for the soil borne fungi. Trichoderma species is the most exploited biocontrol agent in recent years. The soil specific nature of Trichoderma species is a well-known fact and hence native Trichoderma isolates should be more emphasized for control of plant pathogens. Fifty soil samples from rhizosphere of various agricultural crops were collected for isolation of Trichoderma sp. Ten isolates of Trichoderma were tested in dual culture with soil borne pathogens Fusarium solani, Rhizoctonia solani and Sclerotinia sclerotiorum in an in vitro assay. All of the test isolates were found to be significant in terms of mycelial inhibition growth as compared to control. However, varying degrees of antagonism by different Trichoderma isolates were observed for above mentioned soil borne pathogens. The isolate (T363) was found to exhibit more than 80% inhibition of S. sclerotiorum while the isolate T357 was found to control F. solani by more than 80%.  For the control of R. solani, six of the tested Trichoderma isolates showed more than 80% inhibition of its radial colony growth. The Trichoderma isolates seen effective in this study need to be tested in pot and field experiments for exploiting the use and benefits of biocontrol.

Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves

1981 ◽  
Vol 59 (5) ◽  
pp. 826-830 ◽  
Author(s):  
L. A. Mroginski ◽  
K. K. Kartha ◽  
J. P. Shyluk
Keyword(s):  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Environmental Conditions ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Bud Regeneration ◽  
Arachis Hypogaea L ◽  
Murashige And Skoog Medium ◽  
Germinated Seeds

The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested.

In Vitro Culture of Arachis hypogaea Peg Tips1

1992 ◽  
Vol 19 (2) ◽  
pp. 78-82 ◽  
Author(s):  
Tallury P. S. Rau ◽  
H. T. Stalker ◽  
H. E. Pattee ◽  
P. Reece
Keyword(s):  
Arachis Hypogaea ◽  
Interspecific Hybrid ◽  
Embryo Rescue ◽  
Basal Medium ◽  
Early Growth ◽  
Growth Stages ◽  
Globular Stage ◽  
Arachis Hypogaea L ◽  
Indole 3 Acetic Acid

Abstract Arachis hypogaea L. cv. NC 4 was used as a model plant system in an effort to develop an in vitro embryo rescue protocol which could have application to interspecific hybrid embryos, which often abort at very early growth stages. Embryo growth and development was studied in 1- to 4-day-old peg tips containing proembryos equivalent to a stage where many interspecific hybrid embryos abort. Three independent experiments were conducted to 1) determine the most favorable basal media, 2) evaluate the effects of auxins and cytokinins on growth, and 3) determine a favorable combination of auxins and cytokinins for in vitro peanut embryo growth. The results indicated that MS (Murashige and Skoog) medium with 3% sucrose was the most favorable basal medium among seven media and two sucrose concentrations analyzed. IAA (indole-3-acetic acid) at 1.5 mg L-1 in combination with a range of KN (kinetin) levels from 0.5 to 1.25 mg L-1 were the growth regulator combinations of choice. Proembryo growth reached the multicellular globular stage, but differentiation into heart-shaped embryos did not occur.

scholarly journals Phytostimulation and growth promotion activities of Trichoderma spp. on groundnut (Arachis hypogaea L.) crop

2021 ◽  
Vol 13 (4) ◽  
pp. 1172-1179
Author(s):  
M. Ayyandurai ◽  
R. Akila ◽  
K. Manonmani ◽  
M. Theradimani ◽  
S. Vellaikumar
Keyword(s):  
Arachis Hypogaea ◽  
Growth Promotion ◽  
Pcr Amplification ◽  
Its Region ◽  
Germination Percentage ◽  
High Yield ◽  
Trichoderma Spp ◽  
Soil Borne Pathogens ◽  
Trichoderma Sp ◽  
Arachis Hypogaea L

Groundnut (Arachis hypogaea L.) suffers from many soil borne pathogens that deteriorate the quality of the seeds and are responsible for high yield loss. Practically Trichoderma sp. is used for seed treatment, it minimizes the seed and soil borne pathogens and supports plant growth promotion activities. In the present study, five different isolates of Trichoderma spp. were isolated from groundnut (A. hypogaea ) rhizosphere soil. All the five isolates were confirmed by morphological methods and using molecular tools through Polymerase Chain Reaction (PCR) amplification of Internal Transcribed Spacer (ITS) region of Trichoderma sp. and DNA gets amplified in 650 bp to 700 bp. Trichoderma spp. were molecularly identified as T(SP)-20 (Trichoderma longibrachiatum), T(AR)-10 (T. asperellum), T(VT)-3 (T. hamatum), T(BI)-16 (T. longibrachiatum), T(TK)-23 (T. citrinoviride). Phytostimulation activities of all the six isolates viz., phosphate solubilization, Ammonia production, IAA production, and Siderophore production, were evaluated. Among the six isolates, T(SP)-20, T(AR)-10, and TNAU-TA showed higher phytostimulation activities. The growth promotion of Trichoderma spp. on groundnut was assessed through the roll towel method. The isolate T(SP)-20 (T. longibrachiatum) produced the highest germination percentage of 93.33 and vigor index of 2246.2. This work developed a new isolate of T. longibrachiatum (T(SP)-20) which is a native isolate having significant  phytostimulation and growth promotion activities and it could be exploited for other soil borne disease managing successfully.

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