scholarly journals PENGUMPULAN DAN BATAS PEMAKAIAN SAMPEL POPOK PADA PERBENIHAN URIN

2018 ◽  
Vol 12 (2) ◽  
pp. 68
Author(s):  
Rini Riyanti ◽  
Prihatini Prihatini ◽  
M.Y Probohoesodo
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Sample ◽  
Urine Culture ◽  
Practical Method ◽  
Urine Samples ◽  
Culture Result ◽  
Sample Collection ◽  
Infection Diagnosis

Urinary tract infection diagnosis is based on urine culture, taken from a midstream collection in the morning. Obtaining samplesin this manner is difficult in children less than 3 years. In children less than 3 years, urine is obtained by urine collectors. Using urinecollectors may cause discomfort, and the possibility that the urine collectors may not adhere resulting in contamination. (1) Developinga practical method for urine sample collection. (2) Comparing culture from diapers and urine collectors samples. (3) Knowing the limittime for using diapers acceptable for urine culture. Urine samples were obtained from 20 children less than 3 years, using urine collectorsand diapers used for 1 hour, 2 hours and 3 hours and then cultured. Majority of the urine culture from diapers used for 1 hour and 2hours showed the same result with the urine culture from urine collectors. Contamination was found in the urine culture result fromdiapers used for 3 hours. Urine samples from diapers used for 1 hour and 2 hours can be used as samples for urine culture. The techniqueis easy and can be done in children less than 3 years.

scholarly journals Evaluation of BacterioScan 216Dx in Comparison to Urinalysis as a Screening Tool for Diagnosis of Urinary Tract Infections in Children

2019 ◽  
Vol 57 (9) ◽  
Author(s):  
Ferdaus Hassan ◽  
Heather Bushnell ◽  
Connie Taggart ◽  
Caitlin Gibbs ◽  
Steve Hiraki ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Predictive Value ◽  
Screening Tool ◽  
Urine Culture ◽  
Antibiotic Use ◽  
Urine Samples ◽  
Maldi Tof ◽  
Tract Infections

ABSTRACTUrinalysis (UA) has routinely been used as a screening tool prior to urine culture set up. BacterioScan 216Dx is an FDA-cleared semiautomated system to detect bacterial growth in urine. The aim of this study was to evaluate 216Dx in comparison to UA for diagnosis of urinary tract infection (UTI) in children. Clean-catch, unpreserved urine samples from children aged <18 years were tested by 216Dx, and positive urine samples in media were processed for direct bacterial identification by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry. Sensitivity and specificity of 216Dx and urinalysis (UA) were determined against urine culture. Of 287 urine samples obtained from children (median age, 108 months), 44.0% and 56.0% were UA positive and negative, respectively, while 216Dx detected 27% and 73% as positive and negative, respectively. Compared to culture, the overall sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 216Dx versus UA were 92.1% versus 97.3%, 82.7% versus 63.8%, 44.8% versus 29.1%, and 98.6% versus 99.3%, respectively. Among 216Dx true-positive (TP) samples (n= 35), 77.0% were successfully identified directly from broth by MALDI-TOF. Among urine samples that were identified as contaminated by culture (n= 127; 44%), the 216Dx detected 93 (73.0%) as negative while UA detected 69 (54.0%) as negative. Although the sensitivities of 216Dx and UA are comparable, the specificity of 216Dx was higher than that of UA. The 216Dx can be used as an alternative/adjunct screening tool to UA to rule out urinary tract infection (UTI) in children. Compared to culture, the faster turnaround time (3 hours) of 216Dx has the potential to reduce unnecessary antibiotic use and improve patient management.

scholarly journals Microscopic Examination of Urine Samples as the Early Detection of Asymptomatic Urinary Tract Infection in Pregnant Women: A Cross-Sectional Study

2019 ◽  
pp. 208-212
Author(s):  
Yeva Rosana ◽  
Dwiana Ocviyanti ◽  
Rahmah Amran
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Early Diagnosis ◽  
Pregnant Women ◽  
Microscopic Examination ◽  
Urine Culture ◽  
Early Stage ◽  
Urine Samples ◽  
P Value

Abstract Objectives:To investigate the role of microscopic examination of urine sample in supporting early diagnosis of asymptomatic urinary tract infection (UTI) in pregnant women. To compare correspondence between microscopic examination and urine culture result as a gold standard diagnostic modality to support the diagnosis of UTIs and as an evidence-based to start empirical therapy. Methods: Microscopic analysis was conducted in 74 centrifuged and non-centrifuged urine samples from 317 pregnant who came to six healthcare centres in Jakarta, which showed a positive result of nitrite examination.  The results of the microscopic examination of bacteriuria and leukocyturia were compared with a urine culture. Results: Sensitivity of centrifuged bacteriuria was the highest among the other microscopic parameters, which was 74% with the p-value of 0.009. Combination of bacteriuria and leukocyturia≥3/HPF dan≥5/HPF have increased the specificity with the value of 91.5% and 93.6% in non-centrifuged urine. Conclusion: This result showed that the best method of microscopic examination for early diagnosis of asymptomatic urinary tract infection in pregnant women is the detection of bacteriuria in centrifuged urine. Combination of bacteriuriaandleukocyturiatest, as well as leukocyturia≥3/HPF and≥5/HPF,  can be used to rule out the diagnosis of UTI at an early stage. Keywords: asymptomatic urinary tract infection, bacteriuria, leukocyturia,microscopic examination, urine culture.   Abstrak Tujuan: Mengetahui peran pemeriksaan mikroskopis sampel urin dalam mendukung diagnosis dini infeksi saluran kemih asimptomatik (ISK) pada perempuan hamil. Membandingkan kesesuaian antara pemeriksaan mikroskopis dan hasil kultur urin sebagai modalitas diagnostik standar emas untuk mendukung diagnosis ISK dan sebagai dasar bukti untuk memulai terapi empiris. Metode: Penelitian uji mikroskopik dilakukan pada 74 sampel urin disentrifugasi dan tidak sentrifugasi, dari 317 sampel urin perempuan hamil yang berobat ke-enam puskesmas di Jakarta dengan uji nitritpositif. Hasil uji mikroskopik bakteri uria dan leukosit uria dibandingkan dengan hasil kultururin. Hasil: Sensitivitas bakteriuria yang disentrifugasi menunjukan hasil yang paling baik dibandingkan dengan parameter uji mikroskopik lain, yaitu 74% dengan nilai p yang bermakna sebesar 0,009.  Kombinasi bakteri uria dan leukosituria ≥3/LPB dan ≥5/LPB dapat meningkatkan spesifisitas uji dengan nilai 91,5% dan 93,6% pada urin yang  tidak disentrifugasi. Kesimpulan: Hasil menunjukkan bahwa bakteri uria pada urin yang disentrifugasi, merupakan metode yang paling baik untuk membantu diagnosis dini ISK tidak bergejala pada perempuan hamil. Uji kombinasi bakteri uria dan leukosituria, serta uji leukosituria ≥3/LPB dan ≥5/LPB dapat dimanfaatkan untuk membantu secara dini menyingkirkan orang yang tidak mengalami  ISK. Kata kunci: bakteriuria, infeksi saluran kemih tidak bergejala, kultur urin,leukosituria, uji mikroskopik

scholarly journals Prevalence of urinary tract infection in febrile children of less than five years of age

2021 ◽  
Vol 7 (4) ◽  
pp. 205-211
Author(s):  
Mahishma K ◽  
Anil Kumar K
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Sample ◽  
Urine Analysis ◽  
Urine Culture ◽  
College Teaching ◽  
Febrile Illness ◽  
Research Centre ◽  
Upper Respiratory Tract

To determine the prevalence of urinary tract infection in febrile children, less than 5 years of age. To assess the validity of microscopic urine analysis and urine culture in the diagnosis of urinary tract infection.Prospective study. Children attending   the department of paediatrics, Dr.V.R.K. Women’s Medical College, Teaching Hospital & Research Centre, with febrile illness during June 2019-June 2020.370 children between 1 month to 5 years of age.Data related to age, sex, nutritional status, socioeconomic status and predisposing risk factors like urethral instrumentation, bowel habits etc, were noted. A thorough physical examination with relevant investigations were carried out in all these patients. Routine urinary microscopy was done in all patients and urine culture was done in those who showed pyuria of &#62;5 pus cells/HPF in centrifuged urine sample.In our study, overall prevalence of UTI was 3.5% in children between 1 month to 5 yrs and 4.1% in children &#60;2yrs and 7% in Children &#60;1 year of age with M:F ratio of 1:1in children &#60;2yrs. Prevalence of culture positivity was 44% in those who showed &#62;10 pus cells/HPF in centrifuged sample of urine and 2.5% in those who showed &#62;5pus cells/HPF.The presence of obvious source of fever such as upper respiratory tract infection or otitis media is not reliable in excluding urinary tract infection. Overall prevalence of UTI in our study was low (3.5%) and prevalence among children &#60;2yrs was 4.1% and &#60;1 year of age was 7%. Pyuria of &#62;5pus cells/HPF (centrifuged urine sample) should be considered as significant and further evaluation should be done to initiate prompt treatment.

Antibiotic-Resistant Corynebacterium jeikeium Urinary Tract Infection in a Cat

2007 ◽  
Vol 43 (1) ◽  
pp. 61-64 ◽  
Author(s):  
Michelle Puskar ◽  
Carol Lemons ◽  
Mark G. Papich ◽  
Shelley L. Vaden ◽  
Adam Birkenheuer
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Sample ◽  
Urine Culture ◽  
Gene Sequencing ◽  
Antibiotic Resistant ◽  
Sample Contamination ◽  
History Of ◽  
Tract Disease

A 10-year-old, castrated male, domestic longhaired cat with a history of urinary tract disease and perineal urethrostomy was presented for evaluation of persistent urinary tract inflammation. Prior to referral, diphtheroid organisms had been cultured from a urine sample obtained by cystocentesis, and they were interpreted as sample contamination. Subsequent urine culture and gene sequencing identified Corynebacterium jeikeium, which was resistant to antibiotics and appeared to be the cause of the urinary tract infection.

scholarly journals Frequency of Urinary Tract Infection in Children with Diarrhea

2021 ◽  
Vol 15 (6) ◽  
pp. 1885-1887
Author(s):  
Ali Akbar ◽  
Abdul Moeed Khan ◽  
Asadullah Khan ◽  
Farhana Ahmad ◽  
Muhammad Akram ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Medical History ◽  
Study Design ◽  
Urine Culture ◽  
Urine Samples ◽  
Cross Sectional ◽  
Severe Diarrhea ◽  
Keywords Urinary Tract Infection

Aim: To determine the frequency of UTI in children presented with diarrhea. Study Design: Cross-sectional Place and Duration: Pediatrics department of Alkhidmat Hospital, Peshawar and Sharif Medical and Dental College, Lahore for six months duration from October 2020 to March 2021. Methodology: Total 120 patients of both gender with ages 2 months to 5 years were enrolled in this study. After taking written consent from parents/guardians, detailed demographics including age, sex, residence, severity and complete medical history were examined and recorded. Urine samples were obtained for examination of urine culture. Data was analyzed by SPSS 24.0. Results: There were 78 (65%) males while 42 (35%) patients were females. 58 (48.33%) patients were ages <1 years, 45 (37.5%) had ages between 1 to 3 years and 17 (14.17%) were ages of >3 years. 36 (30%) patients had mild, 50 (41.67%) had moderate and 34 (28.33%) had severe diarrhea. UTI was found in 40 (33.33%) patients. E.coli was the commonest organism found in 28 (70%) patients followed by K. pneumonia in 12 (30%) patients. Conclusion: Frequency of urinary tract infection was high among children with diarrhea. E.coli was the commonest organism associated with UTI. Keywords: Urinary Tract Infection, Diarrhea, Children

Assessment of Urine Gram Stain and Urine Culture in the Diagnosis of Urinary Tract Infection

2012 ◽  
Vol 3 (2) ◽  
pp. 472-473
Author(s):  
Dr G Sucilathangam Dr G Sucilathangam ◽  
◽  
Dr G Velvizhi Dr G Velvizhi
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Gram Stain

URINARY INFECTION AT DEPARTMENT OF UROLOGY OF HUE UNIVERSITY OF MEDICINE AND PHARMACY HOSPITAL

2018 ◽  
pp. 100-108
Author(s):  
Dinh Khanh Le ◽  
Dinh Dam Le ◽  
Khoa Hung Nguyen ◽  
Xuan My Nguyen ◽  
Minh Nhat Vo ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Tract Infections ◽  
Urine Culture ◽  
Clinical Manifestations ◽  
University Hospital ◽  
E Coli ◽  
Positive Urine ◽  
Tract Infections

Objectives: To investigate clinical characteristics, bacterial characteristics, drug resistance status in patients with urinary tract infections treated at Department of Urology, Hue University Hospital. Materials and Method: The study was conducted in 474 patients with urological disease treated at Department of Urology, Hue Universiry Hospital from July 2017 to April 2018. Urine culture was done in the patients with urine > 25 Leu/ul who have symptoms of urinary tract disease or infection symptoms. Patients with positive urine cultures were analyzed for clinical and bacterial characteristics. Results: 187/474 (39.5%) patients had symptoms associated with urinary tract infections. 85/474 (17.9%) patients were diagnosed with urinary tract infection. The positive urine culture rate was 45.5%. Symptoms of UTI were varied, and no prominent symptoms. E. coli accounts for the highest proportion (46.67%), followed by, Staphycoccus aureus (10.67%), Pseudomonas aeruginsa (8,0%), Streptococcus faecali and Proteus (2.67%). ESBL - producing E. coli was 69.23%, ESBL producing Enterobacter spp was 33.33%. Gram-negative bacteria are susceptible to meropenem, imipenem, amikacin while gram positive are vancomycin-sensitive. Conclusions: Clinical manifestations of urinary tract infections varied and its typical symptoms are unclear. E.coli is a common bacterium (46.67%). Isolated bacteria have a high rate of resistance to some common antibiotics especially the third generation cephalosporins and quinolones. Most bacteria are resistant to multiple antibiotics at the same time. Gram (+) bacteria are susceptible to vancomycin, and gram (-) bacteria are susceptible to cefoxitin, amikacin, and carbapenem. Key words: urinary tract infection

Urinary Tract Infection

1979 ◽  
Vol 1 (5) ◽  
pp. 133-136
Author(s):  
Richard H. Rapkin
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Underlying Disease ◽  
Chief Complaint ◽  
Voiding Cystourethrogram ◽  
Intravenous Pyelogram ◽  
Rapid Generation

The identification of urinary tract infection (UTI) is important in order to reduce its morbidity, to prevent its sequelae, and to identify underlying disease. This article will discuss methods of diagnosis and management of UTI, screening for UTI, and the importance of further evaluation and follow-up of children with UTIs. Much of what we know about UTI is controversial and rapid generation of new knowledge may make current recommendations passé. CASE V.M., a 4-year-old girl, was brought to the physician's office with the chief complaint of frequency of urination. Nine months before she had been seen because of frequency and dysuria and two consecutive midstream urine cultures grew &gt;100,000 colonies/ml of a Gramneative rod. Sulfisoxazole was begun and a urine culture was sterile 48 hours after therapy was begun. The dysuria and frequency disappeared; therapy was continued for ten days and a urine culture four days later was sterile. One week later a voiding cystourethrogram (VCU) and an intravenous pyelogram (IVP) were performed and were interpreted as normal. Repeat urine cultures at one, two, three, and six months after the episode were sterile. Two days before the child was seen, she had become irritable and wet the bed during sleep (she had been successfully trained at 27 months of age), and she began to void frequently during the next 24 hours.

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