scholarly journals Effect of Qianjinjiu on the Contraction of Gastrocnemius muscle of Isolated Sciatic Nerve

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Jianqing Zhu ◽  
Zhanying Qi ◽  
Chaohui Lin
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Treatment Time ◽  
Salt Water ◽  
Water Extract ◽  
Treatment Level ◽  
Muscle Contractility ◽  
Time Point

Objective: To investigate the water extract from Radix (Flemingia philippinensis Merr et Rolfe Decoction.) Effect of sciatic nerve gastrocnemius muscle Characteristics of isolated frog. Methods: The dissected lesion of sciatic nerve gastrocnemius muscle was infused with 0.9% saline, 0.125g / mL, 0.25g / mL, 0.5g / mL jelly extract, and the RM6240 bio-signal collector was used to measure each The infiltration depth of 5 minutes, infiltration for 10 minutes, infiltration of 15 minutes at each time point of the gastrocnemius muscle contraction threshold strength (measured at each time point 5 times) and complete single contraction curve (measured at each time point 25) Systolic rate, systolic rate, rate of contraction, and latency. Results: The systolic threshold intensity of the gastrocnemius muscle increased with the increase of the concentration of the extraction fluid, but less than the pre-treatment level (P <0.01, P <0.05). If the concentration and time (P <0.05). At the same time, the systolic amplitude, systolic rate and diastolic rate increased first and then decreased with the increase of the concentration of the extract (P <0.01, P <0.05). The latency of the sciatic nerve of the sciatic nerve was affected by the concentration of the drug in the infiltration solution, which decreased first and then increased with the increase of the drug concentration. Conclusion: The systolic characteristics of skeletal muscle are affected by the weight of the components. Low concentration of salt water extract in a short time to improve the ability of skeletal muscle contraction, treatment time is too long or too high concentration of crude drugs will make the skeletal muscle contractility weakened, and even inhibit the skeletal muscle contractility, and affect the nerve Muscle excitement signal transmission.

scholarly journals Changes in glucose 1,6-bisphosphate content in rat skeletal muscle during contraction

1986 ◽  
Vol 240 (3) ◽  
pp. 747-751 ◽  
Author(s):  
A M Bassols ◽  
J Carreras ◽  
R Cussó
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Lactate Concentration ◽  
Glycolytic Flux ◽  
Rat Skeletal Muscle ◽  
Contracting Muscle ◽  
Apparent Relationship

Glucose 1,6-bisphosphate, fructose 2,6-bisphosphate, glycogen, lactate and other glycolytic metabolites were measured in rat gastrocnemius muscle, which was electrically stimulated in situ via the sciatic nerve. Both the frequency and the duration of stimulation were varied to obtain different rates of glycolysis. There was no apparent relationship between fructose 2,6-bisphosphate content and lactate accumulation in contracting muscle. In contrast, glucose 1,6-bisphosphate content increased with lactate concentration during contraction. It is suggested that the increase in glucose 1,6-bisphosphate could play a role in phosphofructokinase stimulation and in the activation of the glycolytic flux during muscle contraction.

Modulation effects of cordycepin on the skeletal muscle contraction of toad gastrocnemius muscle

2014 ◽  
Vol 726 ◽  
pp. 9-15 ◽  
Author(s):  
Li-Hua Yao ◽  
Wei Meng ◽  
Rong-Feng Song ◽  
Qiu-Ping Xiong ◽  
Wei Sun ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Skeletal Muscle Contraction

Specificity of Muscle Reinnervation following Repair of the Transected Sciatic Nerve

1992 ◽  
Vol 17 (3) ◽  
pp. 257-261 ◽  
Author(s):  
Q. ZHAO ◽  
L. B. DAHLIN ◽  
M. KANJE ◽  
G. LUNDBORG
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Statistical Significance ◽  
Nerve Repair ◽  
Nerve Graft ◽  
The Other ◽  
Muscle Contractility ◽  
Tetanic Force ◽  
Rat Sciatic Nerve ◽  
Muscle Reinnervation

Specificity of muscle reinnervation and the recovery of muscle contractility were studied after repair of the transected rat sciatic nerve. Six different techniques were compared: epineurial suture, perineural suture, whole nerve graft, interfascicular grafts, skeletal muscle bridge and tubulization. Muscle tetanic force and specificity of reinnervation were evaluated 12 weeks after nerve repair. Recovery of tetanic force was superior after repair with epineurial sutures. There was no statistical significance between the other methods in respect of tetanic force. The specificity of muscle reinnervation was best after tubulization, repair with interfascicular grafts and perineurial suture.

Inflammatory cells in rat skeletal muscle are elevated after electrically stimulated contractions

2003 ◽  
Vol 94 (3) ◽  
pp. 876-882 ◽  
Author(s):  
Thomas J. McLoughlin ◽  
Eleni Mylona ◽  
Troy A. Hornberger ◽  
Karyn A. Esser ◽  
Francis X. Pizza
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Sciatic Nerve ◽  
High Frequency ◽  
Tibialis Anterior ◽  
Inflammatory Cells ◽  
Muscle Contractions ◽  
Rat Skeletal Muscle ◽  
Concentric Contractions ◽  
Time Point

We determined the effect of muscle contractions resulting from high-frequency electrical stimulation (HFES) on inflammatory cells in rat tibialis anterior (TA), plantaris (Pln), and soleus (Sol) muscles at 6, 24, and 72 h post-HFES. A minimum of four and a maximum of seven rats were analyzed at each time point. HFES, applied to the sciatic nerve, caused the Sol and Pln to contract concentrically and the TA to contract eccentrically. Neutrophils were higher ( P < 0.05) at 6 and 24 h after HFES in the Sol, Pln, and TA muscles relative to control muscles. ED1+ macrophages in the Pln were elevated at 6 and 24 h after HFES and were also elevated in the Sol and TA after HFES relative to controls. ED2+ macrophages in the Sol and TA were elevated at 24 and 72 h after HFES, respectively, and were also elevated in the Pln after HFES relative to controls. In contrast to the TA muscles, the Pln and Sol muscles showed no gross histological abnormalities. Collectively, these data indicate that both eccentric and concentric contractions can increase inflammatory cells in muscle, regardless of whether overt histological signs of injury are apparent.

The α1- and α2-isoforms of Na-K-ATPase play different roles in skeletal muscle contractility

2001 ◽  
Vol 281 (3) ◽  
pp. R917-R925 ◽  
Author(s):  
Suiwen He ◽  
Daniel A. Shelly ◽  
Amy E. Moseley ◽  
Paul F. James ◽  
J. Howard James ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Muscle Contraction ◽  
Extensor Digitorum Longus ◽  
Extensor Digitorum ◽  
Wild Type ◽  
Muscle Contractility ◽  
Functional Roles ◽  
Skeletal Muscle Contraction ◽  
Contractile Parameters

The Na-K-ATPase, which maintains the Na+ and K+ gradients across the plasma membrane, can play a major role in modulation of skeletal muscle contractility. Although both α1- and α2-isoforms of the Na-K-ATPase are expressed in skeletal muscle, the physiological significance of these isoforms in contractility is not known. Evaluation of the contractile parameters of mouse extensor digitorum longus (EDL) was carried out using gene-targeted mice lacking one copy of either the α1- or α2-isoform gene of the Na-K-ATPase. The EDL muscles from heterozygous mice contain approximately one-half of the α1- or α2-isoform, respectively, which permits differentiation of the functional roles of these isoforms. EDL from the α1 +/− mouse shows lower force compared with wild type, whereas that from the α2 +/− mouse shows greater force. The different functional roles of these two isoforms are further demonstrated because inhibition of the α2-isoform with ouabain increases contractility of α1 +/−EDL. These results demonstrate that the Na-K-ATPase α1- and α2-isoforms may play different roles in skeletal muscle contraction.

Cordycepin depresses the skeletal muscle contraction of toad gastrocnemius muscle involving Ca2+-dependent mechanisms

2015 ◽  
Vol 19 (sup8) ◽  
pp. S8-705-S8-709
Author(s):  
W. Sun ◽  
L.-P. Huang ◽  
W. Meng ◽  
C. Chen ◽  
J. Chang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Skeletal Muscle Contraction

Lifting the Nebula: Novel Insights into Skeletal Muscle Contractility

Physiology ◽  
2010 ◽  
Vol 25 (5) ◽  
pp. 304-310 ◽  
Author(s):  
Coen A. C. Ottenheijm ◽  
Henk Granzier
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Muscle Weakness ◽  
Muscle Contractility ◽  
Regulation Of Muscle Contraction

Nebulin is a giant protein and a constituent of the skeletal muscle sarcomere. The name of this protein refers to its unknown (i.e., nebulous) function. However, recent rapid advances reveal that nebulin plays important roles in the regulation of muscle contraction. When these functions of nebulin are compromised, muscle weakness ensues, as is the case in patients with nemaline myoptahy.

Slow skeletal muscle myosin-binding protein-C (MyBPC1) mediates recruitment of muscle-type creatine kinase (CK) to myosin

2011 ◽  
Vol 436 (2) ◽  
pp. 437-445 ◽  
Author(s):  
Zhe Chen ◽  
Tong-Jin Zhao ◽  
Jie Li ◽  
Yan-Song Gao ◽  
Fan-Guo Meng ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Muscle Contraction ◽  
Binding Protein ◽  
High Energy ◽  
Functional Coupling ◽  
Muscle Type ◽  
Myosin Binding Protein ◽  
Slow Skeletal Muscle ◽  
Myosin Binding

Muscle contraction requires high energy fluxes, which are supplied by MM-CK (muscle-type creatine kinase) which couples to the myofibril. However, little is known about the detailed molecular mechanisms of how MM-CK participates in and is regulated during muscle contraction. In the present study, MM-CK is found to physically interact with the slow skeletal muscle-type MyBPC1 (myosin-binding protein C1). The interaction between MyBPC1 and MM-CK depended on the creatine concentration in a dose-dependent manner, but not on ATP, ADP or phosphocreatine. The MyBPC1–CK interaction favoured acidic conditions, and the two molecules dissociated at above pH 7.5. Domain-mapping experiments indicated that MM-CK binds to the C-terminal domains of MyBPC1, which is also the binding site of myosin. The functional coupling of myosin, MyBPC1 and MM-CK is further corroborated using an ATPase activity assay in which ATP expenditure accelerates upon the association of the three proteins, and the apparent Km value of myosin is therefore reduced. The results of the present study suggest that MyBPC1 acts as an adaptor to connect the ATP consumer (myosin) and the regenerator (MM-CK) for efficient energy metabolism and homoeostasis.

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