House dust mite trapping kit

2015 ◽  
Vol 11 (1) ◽  
pp. 2950-2953
Author(s):  
Jaw-Ji Tsai
Keyword(s):  
Risk Factors ◽  
Allergic Disease ◽  
House Dust ◽  
House Dust Mite ◽  
Dermatophagoides Pteronyssinus ◽  
Tyrophagus Putrescentiae ◽  
Asthmatic Patients ◽  
Dust Mite ◽  
Household Environment ◽  
Group 2

Examination of dust collected from the homes of asthmatic patients showed there were significant numbers of house dustmites (HDM). The allergens generated from HDM are considered to be among the risk factors for asthma development.HDM can be identified in mattresses, carpets, quilts and pillows. HDM are about 0.2 x 0.3 mm in size, so they are onlyclearly visible under microscopic magnification. Therefore, it is important to develop a trapping kit to capture and stain them.We devised a trapping kit that is comprised of an adhesive pad and a staining agent. The adhesive pad is made of cottoncloth and is coated with glue, which can attract and trap HDM to prevent them from further spreading. The staining agentcontains nanogold-coupled monoclone antibodies, which can react with HDM through binding with group 2 allergens on thebody surface of HDM. After staining, both Dermatophagoides pteronyssinus (Dp) and Tyrophagus putrescentiae (Tp)become visible to the naked eye.Our study showed that the HDM trapping pad can attract and trap HDM. The stainingagent makes them visible so they can be counted. This kit can help us to identify the predominant HDM infestation area inthe household environment. By identifying the infestation area, we can reduce HDM exposure and prevent the developmentof allergic disease.

scholarly journals Immunoglobulin E to allergen components of house dust mite in Korean children with allergic disease

2015 ◽  
Vol 5 (3) ◽  
pp. 156 ◽  
Author(s):  
Hwan Soo Kim ◽  
Sung Hee Kang ◽  
Sulmui Won ◽  
Eu Kyoung Lee ◽  
Yoon Hong Chun ◽  
...  
Keyword(s):  
Allergic Disease ◽  
House Dust ◽  
House Dust Mite ◽  
Immunoglobulin E ◽  
Korean Children ◽  
Allergen Components ◽  
Dust Mite

scholarly journals Association between serum zonulin level and severity of house dust mite allergic asthma

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Shereen A. Baioumy ◽  
Aya Elgendy ◽  
Shereen M. Ibrahim ◽  
Sara I. Taha ◽  
Shaimaa H. Fouad
Keyword(s):  
Allergic Asthma ◽  
Intestinal Permeability ◽  
House Dust ◽  
House Dust Mite ◽  
Control Group ◽  
Asthmatic Patients ◽  
Mite Allergy ◽  
Significant Difference ◽  
Dust Mite ◽  
Barrier Defect

Abstract Background Increased intestinal permeability, either due to the exposure to antigens in asthmatic patients or due to a barrier defect, plays a critical role in susceptibility to environmental allergens. House dust mite allergy occurs more commonly than any other type of allergy among Egyptian asthmatic patients. Aim To assess the relation between serum zonulin level as a marker of increased intestinal permeability and the severity of house dust mite allergic asthma. Methods A case–control study which included 48 patients with house dust mite allergic asthma and 48 healthy control subjects attending the Allergy and Immunology Unit, Microbiology and Immunology Department, Faculty of Medicine, Zagazig University. Results A statistically significant difference was detected between the two studied groups with respect to serum IgE and serum zonulin levels (p ˂ 0.001 and ˂ 0.001, respectively). The mean serum zonulin was equal to 258.3 ± 153.01 ng/ml in the asthmatic group and 80 ± 13 ng/ml in the control group. Serum zonulin level significantly increased with the increase of asthma severity (p ˂ 0.001). The cut off value of serum zonulin was ≥ 198 ng/ml, and the area under the curve was 0.76. It displayed sensitivity equal to 80% and specificity equal to 71.4%. Its negative predictive value was equal to 83.3%. Conclusion Intestinal barrier dysfunction contributes to the pathogenesis of allergic asthma. Serum zonulin level reflects an increase in intestinal permeability. Zonulin acts as prognostic factor of severity in asthma. Correction of the gut barrier defect may have a potential positive prognostic effect in asthma.

scholarly journals PI3Kδ Inhibition Prevents IL33, ILC2s and Inflammatory Eosinophils in Persistent Airway Inflammation

2021 ◽  
Author(s):  
Sorif Uddin ◽  
Augustin Amour ◽  
David J Lewis ◽  
Chris D Edwards ◽  
Matthew G Williamson ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Goblet Cell ◽  
House Dust ◽  
House Dust Mite ◽  
Inflammatory Responses ◽  
Lymphoid Cells ◽  
Antibody Treatment ◽  
Dust Mite ◽  
Group 2 ◽  
Goblet Cell Metaplasia

Abstract Background: Phosphoinositide-3-kinase-delta (PI3Kδ) inhibition is a promising therapeutic approach for inflammatory conditions due to its role in leucocyte proliferation, migration and activation. However, the effect of PI3Kδ inhibition on group-2-innate lymphoid cells (ILC2s) and inflammatory eosinophils remains unknown. Using a murine model exhibiting persistent airway inflammation we sought to understand the effect of PI3Kδ inhibition, montelukast and anti-IL5 antibody treatment on IL33 expression, group-2-innate lymphoid cells, inflammatory eosinophils, and goblet cell metaplasia. Results: Mice were sensitised to house dust mite and after allowing inflammation to resolve, were re-challenged with house dust mite to re-initiate airway inflammation. ILC2s were found to persist in the airways following house dust mite sensitisation and after re-challenge their numbers increased further along with accumulation of inflammatory eosinophils. In contrast to montelukast or anti-IL5 antibody treatment, PI3Kδ inhibition ablated IL33 expression and prevented group-2-innate lymphoid cell accumulation. Only PI3Kδ inhibition and IL5 neutralization reduced the infiltration of inflammatory eosinophils. Moreover, PI3Kδ inhibition reduced goblet cell metaplasia. Conclusions: Hence, we show that PI3Kδ inhibition dampens allergic inflammatory responses by ablating key cell types and cytokines involved in T-helper-2-driven inflammatory responses.

scholarly journals House dust mite sensitisation and association with atopic dermatitis in Brunei

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Haziq Emran ◽  
Christina Siew Eng Chieng ◽  
Surita Taib ◽  
Anne Catherine Cunningham
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Retrospective Cohort ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
Food Allergens ◽  
Dust Mite ◽  
Storage Mite ◽  
Tropical Areas

AbstractIgE sensitisation in tropical areas is under-reported. A 2 year retrospective cohort study of allergy data specific to aero and food allergens in Brunei demonstrated that specific IgE levels to house dust mite (Dermatophagoides pteronyssinus, D.farinae, Blomia tropicalis) were highest in this population and correlated with atopic dermatitis (p < 0.001). Shrimp and peanut were the most common food allergens. A dominance of house/storage mite sensitization is seen in Brunei which is consistent with other tropical countries.

scholarly journals Mechanisims of asthma and allergic disease – 1085. Safety and tolerability of escalating doses of house dust mite peptide antigen desensitisation

2013 ◽  
Vol 6 ◽  
pp. P81
Author(s):  
Roderick Peter Hafner ◽  
Mark Larché ◽  
Paul Laidler ◽  
Pascal Hickey ◽  
Jacques Hébert
Keyword(s):  
Allergic Disease ◽  
House Dust ◽  
House Dust Mite ◽  
Peptide Antigen ◽  
Dust Mite
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