scholarly journals Detection of luxS gene expression under stressing factors for biofilm formation by Propionibacterium acidipropionici and Propionibacterium freudenreichii

2015 ◽  
Vol 5 (2) ◽  
pp. 604-613 ◽  
Author(s):  
Danitza Xiomara Romero ◽  
Oscar Víctor Cárdenas Alegría ◽  
Víctor Hugo Cavero Olguin ◽  
María Teresa Álvarez Aliaga
Keyword(s):  
Gene Expression ◽  
Biofilm Formation ◽  
Fluorescent In Situ Hybridization ◽  
Culture Conditions ◽  
Propionibacterium Freudenreichii ◽  
Pathogenic Microorganisms ◽  
Luxs Gene ◽  
Propionibacterium Acidipropionici ◽  
Biofilm Reactors

Gene expression constitutes an important role in cellular communication, setting mechanisms for biofilm formation. Genes can be used as molecular markers to monitor viability, stability and maintenance of biofilm eg. in biofilm reactors, bioremediation and biotransformation frequently under stressing conditions to enhance or limit the biofilm formation. In the present study, no pathogenic microorganisms of industrial interest were used. Propionibacterium freudenreichiisubsp. shermanii DSM 4902T and P. acidipropionici DSM 4900Tstrains cannot produce biofilm in culture conditions previously reported. In this regard, chemical culture conditions were modified to stimulate biofilm formation in both strains and determine that under stressing conditions such as 0.6 M NaCl, 1.8 M glucose and 10 gL-1 yeast extract both Propionibacterium produce biofilm. Finally, luxS expression was identified in biofilm of both strains by modified fluorescent in situ hybridization expression (FISH expression).

scholarly journals High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
V. Shablii ◽  
M. Kuchma ◽  
H. Svitina ◽  
I. Skrypkina ◽  
P. Areshkov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cells ◽  
Placental Tissue ◽  
First Trimester ◽  
Mesenchymal Cells ◽  
Culture Conditions ◽  
Multipotent Cells ◽  
Genes Expression

The purpose of this study was to investigate the immunophenotypes and gene expression profile of high proliferative placenta-derived multipotent cells (PDMCs) population at different stages of culture. We demonstrated that the colonies resulting from single cells were either positive or negative for CK7, whereas only PDMC clones with weak CK7 expression (CK7low-clones) were highly proliferative. Interestingly, vimentin positive (Vim+) placental stromal mesenchymal cells did not express CK7 in situ, but double CK7+Vim+ cells detection in tissue explants and explants outgrowth indicated CK7 inducible expression in vitro. PCNA presence in CK7+Vim+ cells during placental explants culturing confirmed belonging of these cells to proliferative subpopulation. Transcription factors CDX2 and EOMES were expressed in both CK7low-clones and subset of stromal mesenchymal cells of first-trimester placental tissue in situ. Meanwhile, CK7low -clones and stromal mesenchymal cells of full-term placental tissue in situ expressed ERG heterogeneously. SPP1, COL2A1, and PPARG2 mesodermal-related genes expression by CK7low-clones additionally confirms their mesenchymal origin. Inherent stem cell-related gene expression (IFTM3, POU5F1, and VASA) in CK7low-clones might indicate their enrichment for progenitors. Finally, in CK7low-clones we observed expression of such trophoblast-associated genes as CGB types I and II, fusogenic ERVW-1, GCM1, and GATA3. Thus, our results indicate that PDMCs acquired the representative immunophenotype signature under culture conditions.

scholarly journals Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Shyam Solanki ◽  
Gazala Ameen ◽  
Jin Zhao ◽  
Jordan Flaten ◽  
Pawel Borowicz ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization

ZebraFISH: Fluorescent In Situ Hybridization Protocol and Three-Dimensional Imaging of Gene Expression Patterns

Zebrafish ◽  
2006 ◽  
Vol 3 (4) ◽  
pp. 465-476 ◽  
Author(s):  
Monique C.M. Welten ◽  
Simon B. de Haan ◽  
Niels van den Boogert ◽  
Jasprien N. Noordermeer ◽  
Gerda E.M. Lamers ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Expression Patterns ◽  
Three Dimensional ◽  
Gene Expression Patterns ◽  
Three Dimensional Imaging ◽  
Hybridization Protocol

Prediction of response to bortezomib and dexamethasone resistance in myeloma (MM) by novel mutations in the NFKB pathway

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 8007-8007
Author(s):  
W. J. Chng ◽  
J. J. Keats ◽  
M. Chesi ◽  
A. Baker ◽  
L. K. Bruhn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fluorescent In Situ Hybridization ◽  
Randomized Study ◽  
Proteasome Inhibitors ◽  
Oligonucleotide Array ◽  
Comparative Genomic ◽  
Large Gene ◽  
Dexamethasone Resistance ◽  
Nfkb Pathway

8007 Background: We recently described mutations in multiple genes in MM patients that lead to the activation of the non- canonical NF-KB pathway. Among the mutated genes, TRAF3 is the most commonly affected, and is inactivated in about 20% of patients. In this study, we further defined the clinical significance of TRAF3 inactivation in MM. Methods: Deletion of TRAF3 was identified by array- based comparative genomic hybridization using Agilent Human Genome CGH 44k oligonucleotide array and confirmed by fluorescent in situ hybridization. Mutations were detected by sequencing. We use a TRAF3 gene expression cut-off (derived from 127 Mayo clinic patients) as a surrogate for TRAF3 inactivation to investigate its association with Translocations and Cyclin D genetic subtypes, response rates, and survival using 2 large gene expression dataset: UAMS dataset (559 newly diagnosed patients treated with Total therapy II and III) and Millenium datasets (213 relapsed patients entered into randomized study of bortezomib versus dexamethasone). Results: A normalized expression level of less than 0.6 identified all but three of the known TRAF3 inactivated cases in the Mayo dataset (p<0.0001). Using this cut-off, TRAF3 inactivated cases were enriched for t(4;14) (26%, p=0.00002) and rarely D1 (mostly hyperdiploid) (9%, p=9.6E-10) in all 3 datasets. TRAF3 inactivation did not affect survival of patients treated with transplant-based therapy (UAMS and Mayo dataset) but was strongly associated with bortezomib response (89% TRAF3 low versus 40% TRAF3 normal, p0.6 had similar PFS with the two treatment. Conclusion: Mutations resulting in TRAF3 inactivation are common, particularly in non-hyperdiploid MM, and predict for response to bortezomib and resistance to dexamethasone suggesting that inhibition of the non-canonical NF-KB pathway may be one of the principle mechanisms of action of proteasome inhibitors in MM. No significant financial relationships to disclose.

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