scholarly journals The Induction of High-Frequency Callus with an Elite Cotton (Gossypium Hirsutum L.) from Côte d'Ivoire

2019 ◽  
Vol 8 ◽  
pp. 1104-1117
Author(s):  
Affoué Sylvie Rachelle N'GUESSAN ◽  
Koutouan AYOLIE ◽  
Sopie Edwige Salomé YAPO ◽  
Yao Kouakou Francois KONAN ◽  
Achi Laurent N’CHO ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Callus Induction ◽  
High Frequency ◽  
Culture Medium ◽  
Carbon Sources ◽  
The Other ◽  
High Quality ◽  
Gossypium Hirsutum L ◽  
Hypocotyl Explants ◽  
Cotton Cultivar

In cotton, factors such as carbon sources, hormonal combination, and type of explant influence the responses to callus induction. In this study callus induction was optimized to get high-frequency callus in cotton. Different carbon sources were tested, and among them, glucose at 3% was found to be the best carbohydrate in callus induction. Culture medium supplemented with 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1) gave a high callogenesis response, unlike other hormonal combinations. With explants, hypocotyl (72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%). So, carbone sources, hormone, and their concentration affect significantly callus induction. The highest frequency of callus induction was then observed when hypocotyl explants were cultured on medium supplemented with 0.1 mg.L-1 of 2,4-D in combination with 0.5 mg.L-1 of KIN and 3% glucose. The induced-callus obtained were friable and of high quality. The other types of combinations resulted in the formation of compact callus with sometimes roots emerging from it. The present study may provide an efficient protocol to improve the callogenesis responses by adjusting the carbohydrates, the hormonal regime, and the type of explant to be cultured for a high-frequency callus induction of the Y331B-R5 cotton cultivar.  

scholarly journals Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2

2015 ◽  
Vol 7 ◽  
pp. 72-80 ◽  
Author(s):  
G. Prem Kumar ◽  
Sivakumar Subiramani ◽  
Siva Govindarajan ◽  
Vinoth Sadasivam ◽  
Vigneswaran Manickam ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Callus Culture ◽  
High Frequency ◽  
Carbon Sources ◽  
Gossypium Hirsutum L

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker�310�FR)

2003 ◽  
Vol 21 (10) ◽  
pp. 955-960 ◽  
Author(s):  
B. Chaudhary ◽  
S. Kumar ◽  
K. V. S. K. Prasad ◽  
G. S. Oinam ◽  
P. K. Burma ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
High Frequency ◽  
Somatic Embryos ◽  
Gossypium Hirsutum L

scholarly journals Antagonistic Potentiality of Some Soil Fungi Against Six Fungal Pathogens Isolated From Cotton (Gossypium Hirsutum L.) Seeds

2021 ◽  
Vol 46 (2) ◽  
pp. 143-153
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
Abul Bashar
Keyword(s):  
Gossypium Hirsutum ◽  
Growth Inhibition ◽  
Radial Growth ◽  
Fungal Pathogens ◽  
Soil Fungi ◽  
The Other ◽  
Dual Culture ◽  
Gossypium Hirsutum L ◽  
Volatile Metabolites ◽  
Other Hand

The antagonistic potentials of four soil filamentous fungi viz., Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. against six pathogens isolated from 14 varieties of cotton (Gossypium hirsutum L.) were evaluated by "dual culture colony interaction”, volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride was found be the most effective to control the growth of the cotton pathogens. T. viride showed the highest growth inhibition against Curvularia lunata, Fusarium moniliforme, Mucor sp. and Rhizoctonia solani. On the other hand A. niger showed the highest growth inhibition against Fusarium nivale and A. fumigatus showed the maximum growth inhibition against C. gloeosporioides. The highest inhibition of radial growth of C. lunata, F. nivale and F. moniliforme was observed might be due to the secretion of volatile metabolites of T. viride whereas, the maximum inhibition of radial growth of C. gloeosporioides was observed because of the volatile metabolites of A. flavus. Mucor sp. and R. solani were inhibited due to the volatile metabolites of A. fumigatus. The greatest radial growth inhibition of C. lunata and F. moniliforme were noticed in case of T. viride owing to the effect of non-volatile metabolites. On the other hand, the radial growth of C. gloeosporioides and F. nivale were inhibited highest amount for the effect of non-volatile metabolites of A. niger, whereas highest growth inhibition of Mucor sp. and R. solani was observed due to the non-volatile effect of A. fumigatus. The present investigation suggests that the isolates of Aspergillus and Trichoderma may be further exploited as potential biocontrol agents against the fungal pathogens of cotton in field trial. Asiat. Soc. Bangladesh, Sci. 46(2): 143-153, December 2020

Aneuploids induced by deficiencies of chromosome 9 and analysis of the time of nondisjunction in cotton

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 12-18 ◽  
Author(s):  
E. L. Myles Jr. ◽  
J. E. Endrizzi
Keyword(s):  
Gossypium Hirsutum ◽  
High Frequency ◽  
Chromosome 9 ◽  
Paraffin Sections ◽  
Stages Of Development ◽  
Gossypium Hirsutum L ◽  
Genome Chromosome ◽  
Significant Difference ◽  
Chromosome Nondisjunction ◽  
Allotetraploid Cotton

Plants of allotetraploid cotton, Gossypium hirsutum L. (2n = 4x = 52), which are monosomic for chromosome 9 or haplodeficient for the long arm of the chromosome, an Ah genome chromosome, produce a high frequency of aneuploid progeny. The aneuploids include monosomes, trisomes, and multiple monosomic and trisomic combinations that are the result of chromosome nondisjunction, which is induced by the deficiency of the long arm of chromosome 9. Loss of chromosomes occurs far more frequently than additions, and chromosomes of the Ah genome undergo nondisjunction at a significantly greater rate than do chromosomes of the Dh genome. Stained paraffin sections of ovules at different stages of development of monotelodisomic 9 and normal plants (TM1) were analyzed to determine the time and probable causes of nondisjunction during megasporogenesis. The most significant difference between mono 9 and TM1 was observed in ovules in stages of development just before and at the time of flowering. The mono 9 female gametophytes had supernumerary nuclei, indicating that chromosome nondisjunction occurs at the mitotic divisions of the embryo sac.Key words: chromosome nondisjunction, aneuploids, megasporogenesis, cotton, Gossypium hirsutum cytogenetics.

Bemisia argentifolii Adult, Nymph and Egg Densities and Egg Distribution on Selected Upland Cottons

2000 ◽  
Vol 35 (1) ◽  
pp. 39-47 ◽  
Author(s):  
C. C. Chu ◽  
T. Freeman ◽  
E. T. Natwick ◽  
J. S. Buckner ◽  
D. R. Nelson ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Upland Cotton ◽  
The Other ◽  
Silverleaf Whitefly ◽  
Bemisia Argentifolii ◽  
Gossypium Hirsutum L ◽  
Egg Distribution ◽  
Leaf Surfaces

Five upland cotton, Gossypium hirsutum L., cultivars, Deltapine (DPL) 50, 5415 and 5432, Fibermax 832 and Siokra L23, were studied in relation to silverleaf whitefly, Bemisia argentifolii Bellows and Perring, oviposition and colonization. Deltapine 5415 and 5432 had the highest numbers of eggs and nymphs and Siokra L-23 the lowest. Siokra L-23 also had the lowest number of adults compared to the other four cultivars. Over 75% of eggs were oviposited on leaf surfaces between veins and 23% were oviposited in veins that were ≤4 cells wide. Few eggs were found on veins that were five or more cells wide. No eggs were inserted into leaf stomata.

Sign in / Sign up

Export Citation Format

Share Document